• Biotechnology and Bioprocess Engineering:BBE
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• 제3권2호
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• pp.109-111
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• 1998

The effect of dissolved oxygen concentration on the metabolism of glucose in Pseudomonas putida BM014 was investigated. Glucose was completely converted to 2-ketogluconate via extracellular oxidative pathway and then taken up for cell growth under the condition of sufficient dissolved oxygen concentration. On the other hand, oxygen limitation below dissolved oxygen tension (DOT) value of 20% of air saturation caused the shift of glucose metabolism from the extracellular oxidative pathway to the intracellular phosphorylative pathway. Specific activities of hexokinase and gluconate kinase in intracellular phosphorylation pathway decreased as the DOT increased, while 2-ketogluconokinase activity in extracellular oxidative pathway increased under the same condition. This result can be usefully applied to microbial transformation of glucose to 2-ketogluconate, the synthetic precursor for iso-vitamine C, with almost 100% yield via extracellular oxidation by simple DOT control.

• 환경위생공학
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• 제15권1호
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• pp.7-13
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• 2000

To develop the removal technique of algal bloom the efficiencies of algal flocculation/ autoflotation by the kinds of coagulant and oversaturated oxygen concentration were investigated. The summarized results are as follow. 1. In the algae flocculation test with alum[$Al_2(SO_4)_3{\cdot}18H_2O$], optimum pH was 5.5 and, with chitosan optimum pH was 7.0. 2. Chitosan which was natural polymer showed the 5~10 times higher algal biomass flocculation efficiency than alum in the condition of same algal concentration. 3. For the each coagulant, the higher ${\Delta}DO$(oversaturated dissolved oxygen concentration - saturated dissolved oxygen) was, the faster the rising velocity of the algal floc was. 4. In the condition of about 4mg/L ${\Delta}DO$, the rising velocity of chitosan is about 2 times higher than that of alum, and chitosan formed the stronger algal floc.

• KSBB Journal
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• 제15권3호
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• pp.280-284
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• 2000

The simple imcroprocessor device was provided for the control of dissolved oxygen(DO) level. The DO analog signal output from fementer was used as a measurement variable and the agitation speed of fermenter was used as a manipulatio variable. Agitation speed was manipulated to maintain DO level above 10% of saturation condition during cell growth period with microprocessor. Since the experimental resultsshowed that the DO level was maintained above 10% and agitation speed was inverse proportional to DO level ithis simple control device can be used for the DO control of fermenter.

• 한국농공학회논문집
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• 제52권5호
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• pp.47-52
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• 2010

The purpose of this study is to investigate characteristics of total phosphorus (TP) and phosphate phosphorous ($PO_4$-P) concentrations in ponded water and redox potential (Eh) in paddy soil during the growing season. The TP and $PO_4$-P concentrations showed twice peak values after basal dressing and tillering fertilization. The ratio of $PO_4$-P to TP showed low values (0.07~0.18), indicating that most of phosphorus is particlulate. The $PO_4$-P concentrations significantly decreased with dissolved oxygen (DO) concentrations. The Eh showed high values (179~636 mV) under non-ponded aerobic condition, but low values (74~112 mV) under ponded anaerobic condition The TP and $PO_4$-P concentrations in ponded water increased shortly after tillering fertilization even if phosphorus was not applied. This may be due to the release of dissolved phosphorus from the bottom sediment and its associated algal and water flea blooms under anaerobic condition. Therefore, proper water management should be needed shortly after tillering fertilization.

• 한국미생물·생명공학회지
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• 제20권3호
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• pp.348-354
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• 1992

SUC2 유전자를 가진 재조합 Saccharomyces cerevisiae의 invertase 생산성을 향상시키기 위하여 균체 생육과 유전자 발현에 미치는 아미노산과 용존산소 농도의 영향을 연구하였다. 균체 생육과 invertase 발현에 적합한 leucine과 histidine의 최적 첨가량은 탄소원인 포도당에 대한 비율로서 나타내어 0.03g/g glucose와 0.04g/g glucose였다. 회분배양에서는 통기량이 적을수록 invertase 비활성이 증가하였다. 희석율 0.09h에서 용존산소농도를 조절한 연속배양에서는 요존산소농도가 감소함에 따라 유전자 발현이 잘되어 5 포화 이하일때 invertase 비활성이 급격히 증가하여 통기를 하지 않은 조건에서 125.54KU/g cell의 최고 비활성을 얻었다.

• KSBB Journal
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• 제13권6호
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• pp.675-680
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• 1998

In both 7L and 20L fermentor experiments the level of dissolved oxygen (D.O) strongly affected growth and PHBV production of Azotobacter vinelandii UWD. A higher D.O. increased carbon substrate consumption rate and cell growth rate with a similar residual biomass production. However, a lower D.O. was a much better condition for PHBV production. In a 20L fermentor experiments controlled at 5% D.O. cell growth rate was about twice faster(0.555 hr-1 and 0.260 hr-1 at the acid and the glucose phase, respectively) with an equal amount(4.5 g/L) of residual biomass production. However, PHBV content in the cell(62.3 wt%) increased 17.3 times at 1% D.O.

• Journal of Microbiology and Biotechnology
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• 제23권1호
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• pp.99-105
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• 2013

In this study, nitrous oxide ($N_2O$) emission was compared between the operations of two different sequencing batch reactors, conventional sequencing batch reactor (CNVSBR) and simultaneous nitrification and denitrification sequencing batch reactor (SND-SBR), using synthetic wastewater. The CNV-SBR consisted of anoxic (denitrification) and aerobic phases, whereas the SND-SBR consisted of a microaerobic (low dissolved oxygen concentration) phase, which was achieved by intermittent aeration for simultaneous nitrification and denitrification. The CNV-SBR emitted 3.9 mg of $N_2O$-N in the denitrification phase and 1.6 mg of $N_2O$-N in the nitrification phase, resulting in a total emission of 5.5mg from 432mg of $NH_4^+$-N input. In contrast, the SND-SBR emitted 26.2mg of $N_2O$-N under the microaerobic condition, which was about 5 times higher than the emission obtained with the CNV-SBR at the same $NH_4^+$-N input. From the $N_2O$ yield based on $NH_4^+$-N input, the microaerobic condition produced the highest yield (6.1%), followed by the anoxic (0.9%) and aerobic (0.4%) conditions. It is thought that an appropriate dissolved oxygen level is critical for reducing $N_2O$ emission during nitrification and denitrification at wastewater treatment plants.

• 한국해안·해양공학회논문집
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• 제33권6호
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• pp.238-245
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• 2021

본 연구에서는 LSTM 모델을 활용하여 낙동강 하구역의 DO 농도 예측을 위한 최적 모델 조건과 적합한 예측변수를 찾기 위한 Case study를 수행하였다. 모델 매개변수 case study 결과, Epoch = 300과 Sequence length = 1에서 상대적으로 높은 정확도를 보였다. 예측변수 case study 결과, DO와 수온을 예측변수로 했을 때 가장 높은 정확도를 보였으며, 이는 DO 농도와 수온의 높은 상관성에 기인한 것으로 판단된다. 상기 결과로부터 낙동강 하구역의 DO 농도 예측에 적합한 LSTM 모델 조건과 예측변수를 찾을 수 있었다.

• 대한토목학회논문집
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• 제41권5호
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• pp.493-504
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• 2021

와류에 의해 물과 산소의 충돌을 반복적으로 일으켜 산소를 용존시키는 Multistage Vortex Aerator를 하천 정화에 적용하였다. 본 연구의 대상 현장인 괴정천은 부산시 사하구 도심을 흘러 낙동강 하구로 연결되는데, 우천 시에는 비점 오염물질이 유입되고 평시에도 하수 슬러지가 일부 유입되어 퇴적을 반복함에 따라 하천수의 용존산소 농도가 혐기성 상태에 가까웠다. 산소농도가 최소 0.1 ppm인 하천수를 흡입하여 22 ppm 이상으로 산소를 용존시켜 분당 1 m³을 순환시켰을 때, 하천에 나타나는 수질과 악취 변화를 2개월간 모니터링하였다. 운영 결과, 용존산소가 하천 전체적으로 약간 나쁨(4)~나쁨(5)에서, 좋음(1b)~보통(3) 등급으로 개선되었고, 총인 농도는 평균 76 % 감소되었다. 복합악취는, 하천 전체가 혐기화된 측정일에 최대 84.5 % 저감을 관찰할 수 있었다.

• 한국물환경학회지
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• 제21권6호
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• pp.595-601
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• 2005

In this research, effects of water circulation on phosphorus release from sediment into water body were studied. Sediments sampled at the Daechung Lake were used for the column experiments with circulation and non-circulation conditions. Deaeration coefficient, $K_1$ and reaeration coefficient, $K_2$ of non-circulation condition were 0.133 and 0, respectively, while $K_1$ and $K_2$ for circulation condition were 0.46 and 0.018, respectively. Oxidation Reduction Potential (ORP) showed a linear relationship with dissolved oxygen (DO) when DO is over 2 mg/L. Phosphorus concentration induced by phosphorus release from sediment was highly dependent upon DO, ORP, and pH. Under anaerobic condition, phosphorus release rate was higher for $Fe^{2+}$-bounded phosphorus compared to that of $Ca^{2+}$-bounded phosphorus.