• Childhood Kidney Diseases
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• 제10권1호
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• pp.8-17
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• 2006

목적 : 생체 외 당뇨병 상태로서 고농도의 당을 포함하는 배양액과 후기당화합물을 적용하여 세포배양하고 이때에 나타나는 병리적 변화, 즉, 세포외 기질의 변화와 형태학적 변화와 함께 투과성(여과율)의 변화를 살펴보았고 동시에 당뇨병성 신증에서의 단백뇨의 기전을 설명하고자 하였다. 방법 : 후기당화합물의 준비를 위해 50mg/mL BSA(Fraction V, Sigma)와 pretense inhibitor를 포함한 PBS(pH 7.4)에 glucose-6-phosphate를 섞어 0.2 M의 용액을 만들었다. BSA를 대조군으로 하였으며, 후기당화합물과 BSA를 $5{\mu}g/cm^2$ surface area의 농도가 되도록 붓고 다음과 같은 비교 대상의 culture dishes를 만들었다(B5; BSA만 첨가 - 5 mM, B30; BSA만 첨가 - 30mM, A5; 후기당화합물만 첨가 - 5 mM, A30; 후기당화합물만 첨가 - 30 mM, A/B 25: osmotic control - 25 mM mannitol). 이틀 배양 후와 일 주 배양 후 각각의 culture dishes에 있는 heparan sulfate proteoglycan (HSPG)양을 ELISA를 이용하여 측정하고 B5를 대준군으로 하여 각각 비교하였다. 각각의 colture dishes에 있는 사구체 상피세포를 scanning EM(Hitachi S-570, Japan)을 이용하여 형태학적 관찰을 하였다. Cellulose semi-permeable membrane을 이용하여 각각의 culture dishes에서 두 시간 동안 apical chamber를 통해 여과되는 BSA양을 sandwich ELISA method로 측정하여 투과성에 대한 분석을 하였다. 결과 : 이틀 동안 배양 후 측정한 대조군을 포함한 다섯 culture dishes의 HSPG양은 통계학적 차이는 없었다. 일 주 배양 후에 이틀 동안 배양한 B5 dish에 비해서 일 주 배양한 A30 dish를 제외한 일 주 배양한 모든 dishes에서 10% 이상의 HSPG양의 증가를 보였다(P<0.05) 일 주 배양한 B5 dish에 비해선 일 주 배양한 A30과 B30 dish에서 각각 HSPG양이 각각 77.8%와 95.3%로 감소하였고(P>0.05), osmotic control group(A/B 25)에선 통계적으로 유의한 차이를 보이지 않았다(P>0.05). 후기당화합물이 첨가된 경우에 SEM상 분리된 세포사이이음(intercellular junction)과 융합된 미세융모를 관찰할 수 있었다. BSA의 투과성은 일 주 배양 후 A30 dish에서만 일 주 배양 후 B5 dish에 비해 19% 증가하는 소견을 보였으나 통계적인 유의성은 없었다(P>0.05). 결론 : 사구체 상피세포의 HSPG 형성의 감소에 고농도의 당과 후기당화합물은 서로 부가적인 역할을 하고 후기당화합물이 더 큰 역할을 함을 알 수 있다. HSPG 감소 소견과 더불어 SEM상 장기간 고혈당을 유지하면 사구체 여과기전에서 size-selective와 charge-selective 장벽에 결함을 유발할 수 있으며 당뇨병에서의 단백뇨의 기전 중 하나로 생각된다.

• 대한가정학회지
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• 제29권1호
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• pp.51-58
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• 1991

This study was conducted to investigate the dietary and nutritional status supplied by group feeding for blue color and white color in Seoul and Kyunggi area. The results are summarized as follows : 1. Blue color and white color of the average food supply per person per day was 1370.0g. Blue color of the total fool supply was consisted of 35.1% grain, 44.1% vegetables. White color of the total fool supply was consisted of 31.8% grain, 44.0% vegetable. The amount of milk product, fruit and fat supply were very small. 2. Blue color and white color of the ratio of main dish and side dish supplied was 69 : 31, 60.0 : 39.7. 3. Main dish all is rice. Side dish ard kinds of soupes and Jigeai Johngol in the two groups. In the kind of side dish white color much more than blue color. Dessert are scarcely supplied in the two groups. 4. The mean daily supplies of energy and 9 nutrients were above the level of the R.D.A. Blue color and white color of the mean daily amount of energy supply was 2653.0㎉ 3022.6㎉. Blue color and white color of the ratio of carbohydrate, protein, and fat supplied was 68.4%, 18.6%, 13.1%, and 62.1%, 20.9%, 17.0%. 5. Blue color and white color of the average feeding cost amounts to 460won, 812won per meal. 6. Blue color; There were high correlations in supply amoung energy, protein, fat. Carbohydrate, calcium, iron, riboflavin, niacin. White color; There were high correlation in supply amoung energy, protein, carbohydrate, calcium, iron, thiamin, riboflavin, niacin, vit-C.

• 한국식생활문화학회지
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• 제14권4호
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• pp.305-317
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• 1999

An attempt was made to provide valuable information to improve service quality and customer satisfaction for in-flight catering service. The customers' demographic characteristics that impact on service quality and customer satisfaction were examined for a group of customer. 474 customers who have been used the airplane within 1 year were participated in this study. Statistical analysis was performed using SAS software program (version 6.01) for descriptive analysis, factor analysis, t-test and ANOVA. The results of the study can be summarized as follows: 1. Service Quality in In-flight catering was assessed on the basis of 18 service quality attributes and 5 dimensions derived from a factor analysis. After comparing customers' perception to customers' expectation regarding in-flight service quality, the quality of dimensions for 'meals', and 'sanitation' were not showed any significant differences. However, the expectation for dimensions of 'diversity of menu', 'providing information', and 'the degree of concern to the customers' were rated significantly higher than the perception for those. 2. Among the demographic variables, age and gender had highly influences on the customers' expectation and perception regarding service quality and customer satisfaction. 3. The korean food that the customers wanted to have for in-flight meals was investigated. Among the kinds of gruel(jook), abalone gruel(jeonbok-jook), pumpkin gruel(hobak-jook), and pine-nut gruel(jat-jook) were chosen to have for in-flight korean meals. It was rated that the preferences for beef soup(sogogikook), hot spicy beef soup(youkgaejang), brown sea-weed soup(miyoukkook) were higher than others in soup group. The preferences for boiled rice with assorted mixture(bibimbab) showed the highest and beef and rice soup(seoleongtang), boiled rice with four the staple cereals(ogogbab) were preferred higher than the others in main dish group. Bulgogi was rated the most popular dish. Korean style punch with omiza(whachae), sweet rice dish(yaksik) and fried glutinous rice cake(gangjeong)showed high preferences in dissert.

• 대한영양사협회학술지
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• 제4권2호
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• pp.188-199
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• 1998

The menu planning is closely tied to the acceptability of the food and the satisfaction of the food service system to its client, therefore national school lunch program(NSLP) menu should consider the food preferences of the students. To provide appropriate information and guidelines about desirable NSLP menu planning, the comparison between the frequency of NSLP menus and food preferences of the students was performed, NSLP menus with cooking method of 776 meals were collected from 10 elementary NSLP schools in Seoul and Kyunggi province, and food preference data was surveyed with 1618 elementary school students from 4 NSLP schools in Korea in 1995. According to the cooking methods, NSLP menus served Kimchi(98.3%), rice(71.4%) and soup(71.1%) frequently. Based on the food groups, NSLP menus served vegetables most frequently(188%), followed by grains(101.4%), milk(100%), meat(57.3%), fruits(50.3%), and so on. By Spearman Rank Correlations analysis, the serving frequencies of vegetable side dishes(r=.33, p=.05) were positively correlated with the students' preference score. According to the cooking method, the serving frequencies of steamed dishes(r=1.0, p=.00), salads(r=1.0, p=.00), Kimchi(r=.85, p=.01) and rice one-dish meal(r=.80, p=.10) were positively correlated with the students' preference score. However, the serving frequencies of the main dishes(r=.13, p=.57) and soup group(r=.15, p=.57) were not significantly correlated with the students' preference score. Even though the serving frequency of fruit were negatively correlated with the students' preference score(r= -.80, p=.10). These results indicated that the NSLP menu planned the vegetable side dishes in accordance with the students' food preferences, but it planned the main dish, soup group, and meat side dish group discordantly. Therefore further efforts are needed to involve the preferred cooking methods for increasing the food acceptability. It could be used for the basic guidelines for menu planning of NSLP, and for the future improvement of NSLP in Korea.

• 대한영양사협회학술지
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• 제12권4호
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• pp.411-431
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• 2006

The purpose of this study was to assess the frequently consumed dish consumption frequencies of continuous ambulatory peritoneal dialysis(CAPD) patients with osteopenia and develop recipes to improve bone mineral density of CAPD. The subjects were 96 CAPD patients with osteopenia(male 39, female 57) (osteopenia group) and 45 CAPD patients with normal BMD(male 24, female 21), matched with key variables(normal group). Fifty dishes(foods) that most frequently consumed were determined and food consumption frequency for each dish(food) for two groups were compared. Osteopenia group showed lower consumption frequency for ice-cream but higher frequency in apple. Of the 50 most frequently consumed dishes(foods), 20 dishes assessed as safe and recommendable for CAPD patients with osteopenia based on the contents of protein and mineral were selected : white boiled rice, white gruel, beef soup, steamed cabbage, roasted dried laver, fried egg, roasted bean-curd, cooked and seasoned bean sprouts, corn-starch jelly, cheese, ice-cream, orange juice, apple, grape, peach, peanut, raw lettuce, raw cucumber, and injulmi rice cake. wenty eight new dishes with modified recipes were developed for CAPD patients. Protein and mineral contents were analyzed for frequently consumed 17 dishes, assessed as modification of recipes are needed. The recipes were modified to decrease P, Na and K contents and to increase protein and Ca contents. Twenty dishes(foods) selected as having reasonable protein and mineral contents ratio or 28 newly developed dishes modified with protein and mineral contents or ratio would be helpful for nutrition education or counseling for CAPD patients with osteopenia. Dishes(foods) suggested in this study would also be useful for all CAPD patients for preventing osteoporosis.

• 대한지역사회영양학회지
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• 제12권5호
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• pp.545-558
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• 2007

This study was performed to assess the sodium intakes of Korean adults using a 24-hr urine analysis and dish frequency questionnaire (DFQ) according to each dish group and the regional area. The subjects of this study were comprised of 522 adults (male : 267, female : 285), aged 20-59yr residing in the metropolitan area (N=200), Chungcheng-Do (N=117), Jeolla-Do(N=117), and Gueongsang-Do provinces (N=118). The subjects were recruited from the residents who once participated or are participating in the various health programs offered by the public health center. The number of subjects who completed the 24-hr urine collection was 205 (male : 110, female : 95). The mean age and BMI of the subjects were $39.0{\pm}$11.7y and $23.1{\pm}2.9 kg/m^2$, respectively. The mean systolic and diastolic blood pressure was $119.5{\pm}15.4 mmHg$, and $77.1{\pm}11.1 mmHg$, respectively. Eighteen percent of the subjects responded that they are currently smoking, 36% drinking and 50.4% exercising. Twenty point six percent of the subjects were assessed as having hypertension according to their systolic or diastolic blood pressure($SBP{\ge}140mmHg$ or $DBP{\ge}90mmHg$) measurements in the present study. Salt intake of the subjects estimated with 24-hr sodium excretion was 12.7g/d (male : 13.4g/d, female : 12.1g/d) based on the sodium excretion rate as 82%. Salt intake estimated with DFQ was 14.7g/d (male : 16.2g/d, female : 13.4g/d), 2 g more than the salt intake estimated with 24-hr urine analysis. The four dish groups that contributed most to the sodium intake in order were kimchi (11571.4mg), soup and stew (1260.5mg), fish and shellfish(706.3mg) and noodle and ramyeon(644.3mg). Salt intake estimated with DFQ was the highest in the subjects of Gueongsang-Do(17.0g/d), second highest Chungcheong-Do (16.4g/d) and the lowest in the metropolitan area (13.0g/d). Subjects of Gueongsang-Do showed the highest sodium intakes in most of the dish group, whereas subjects of the metropolitan area showed the lowest. Residents of Chungcheong-Do revealed the highest sodium intake with kimchi and of Jeolla-Do the higher sodium intake with main dish (meat, fish and beans). The highest salt percentage of kimchi ($3.0{\pm}0.8%$) and soybean paste ($14.5{\pm}5.1%$) were observed in Gueongsang-Do, whereas individuals of the metropolitan area were observed as having kimchi ($1.6{\pm}0.5%$) and soybean paste ($7.4{\pm}1.6%$) with the lowest salt percenage. Men were observed as having more salty kimchi ($2.4{\pm}0.1%$) than women ($2.1{\pm}0.1%$).

• 한국식품영양과학회지
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• 제32권5호
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• pp.764-778
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• 2003

본 연구는 주부(30~59세) 30명을 대상으로 하여 추정량기록법으로 연중 음식 및 식단 패턴을 조사하였으며, 그 결과를 요약하면 다음과 같다. 섭취 빈도를 기준으로 살펴본 상용 음식은 4계절 모두에서 쌀밥, 배추김치, 열무김치, 커피, 김구이, 된장찌개 등이었고, 음식군별 섭취빈도는 밥류, 김치류, 음료류, 과일 및 주스류, 국 및 탕류, 찌개류, 나물 및 무침류 순으로 섭취를 하고 있었다. 앞선 모든 연구에서와 마찬가지로 우리나라의 주요 식생활의 패턴인 밥을 주식으로 하는 전통 식생활을 보여 주고 있다. 음식 군별 섭취량을 보면 국.탕류, 찌개류는 가을, 겨울에, 전.부침류는 원료가 되는 채소류 등 농산물이 많이 생산되는 여름에 가장 많이 섭취하였다. 나물 무침류는 겨울에 가장 많이 섭취하였고 김치류는 4계절 모두 많이 섭취하고 있었다. 가장 자주 섭취한 음식 군은 밥류, 김치류, 음료류, 과일 및 주스류, 국 및 탕류 순이었다. 음식 군별 다빈도 음식을 살펴보면 밥류의 경우 모든 계절에서 쌀밥의 섭취 빈도가 다른 종류에 비해 월등히 높았다. 면.만두류의 경우 모든 계절에서 라면을 가장 많이 섭취하고 있었다. 국.탕류의 경우 봄, 여름, 겨울에서는 미역국, 가을은 배추된장국이었다. 찌개류는 봄과 여름, 겨울에서 된장찌개, 가을의 경우 김치찌개 등의 결과를 보여주었다. 계란찜은 모든 계절에서 섭취량이 가장 많은 것으로 나타났고, 구이류의 경우 김구이가 4계절 모두 1위를 보여주어 가장 많이 먹는 구이류임을 보여 주었다. 전.부침류의 섭취 빈도는 사계절 모두 계란 후라이가 1순위를 차지하였고, 볶음류의 경우 멸치볶음이 4계절 모두 가장 섭취 빈도가 높았고, 조림류는 봄의 경우 콩조림, 여름은 깻잎조림 , 가을과 겨울은 고등어조림을 자주 섭취하는 것으로 나타났다. 나물.무침류는 봄은 취나물, 여름은 콩나물, 가을은 무생채, 겨울은 시금치나물, 김치류는 4계절 모두 배추김치가 섭취 빈도가 높았고, 열무김치와 깍두기, 총각김치도 모든 계절에서 섭취 빈도가 높았다. 조사 대상자들이 가장 많이 섭취하는 상위 10위 권에 드는 대부분의 식단이 밥과 반찬으로 이루어진 식단이었다. 봄.여름.가을 겨울 모두 가장 많이 사용한 식단의 형태는 밥＋국＋김치로 각각 그 비율이 6.84%, 6.10%, 7.18%, 6.84%이었다. 2위는 봄이 밥＋김치(5.56%)인 반면, 나머지 여름, 가을, 겨울은 면(만두)＋김치형태였다. 본 연구는 조사 대상수가 다소 적다는 점이 있지만, 식사를 직접 준비하는 주부가 기록하였고 지금까지의 단기간 조사의 단점을 보완한 365일의 식품 및 음식섭취 자료라는 점에서 의미 있는 자료가 될 수 있을 것이다.

• Journal of Nutrition and Health
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• 제55권6호
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• pp.670-683
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• 2022

본 연구는 한국 비만 성인의 개인의 특성을 고려한 구체적인 가이드라인 마련을 위한 기초자료를 제공하고자, 2013-2018년 국민건강영양조사 자료를 분석하여 비만군을 대상으로 연령에 따라 식품군·음식군 섭취비율 분석하였으며, 그 결과는 다음과 같다. 비만 여부에 따른 총 에너지 섭취 대비 식품군의 섭취 비율을 분석한 결과 남성에서는 곡류, 고기·생선·달걀·콩류, 채소류, 기타로 나타났으며, 여성에서 곡류, 고기·생선·달걀·콩류, 채소류, 우유 및 유제품류, 유지 및 당류, 기타로 나타났다. 비만 여부에 따른 총 에너지 섭취 대비 음식군의 섭취 비율을 분석한 결과 남성에서는 면·만두류, 구이류, 볶음류, 나물·무침류, 회류, 장아찌류, 과일군, 밥류, 빵·과자류, 우유 및 유제품, 음료·차류였으며, 여성에서는 밥류, 면·만두류, 빵류, 구이류, 볶음류, 튀김류, 나물·무침류, 김치류, 회류, 과일군, 떡류, 우유 및 유제품, 음료·차류로 나타났다. 비만 성인에서 연령에 따른 총 에너지 섭취 대비 식품군 섭취비율을 분석한 결과 남성은 연령에 따라 곡류, 고기·생선·달걀·콩류, 채소류, 기타에 유의한 차이를 보이는 것으로 나타났으며, 여성은 곡류, 고기·생선·달걀·콩류, 채소, 우유 및 유제품류, 유지 및 당류에서 연령에 따라 유의한 차이를 보이는 것으로 나타났다. 비만 성인에서 연령에 따른 총 에너지 섭취대비 음식군 섭취비율을 분석한 결과 남성은 연령에 따라 빵·과자류, 면·만두류, 찌개류, 찜류, 구이류, 튀김류, 우유 및 유제품류, 음료·차류에 유의한 차이를 보이는 것으로 나타났으며, 여성은 밥류, 빵·과자류, 면·만두류, 구이류, 볶음류, 튀김류, 김치류, 나물·무침류, 음료·차류, 떡류, 기타에서 연령에 따라 유의한 차이를 보이는 것으로 나타났다. 본 연구 통해 비만 성인에서 연령별 식품군 및 음식군의 섭취 비율을 파악하였으며, 향후 비만 성인의 성별과 연령대를 고려한 식사 섭취 가이드라인의 기초자료로 활용이 기대되는 바이다.

• Reproductive and Developmental Biology
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• 제39권3호
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• pp.49-57
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• 2015

In order to achieve successful in vitro production of embryo, it is necessary to establish intrauterine environment during in vitro culture. Thus, this study was investigated to establish embryo culture system using co-incubated collagen matrix gel (CM) with endometrial epithelial cells (EC). Endometrial epithelial cells were isolated from porcine endometrium at follicular phase, the cells seeded in insert dish for co-incubation with CM-coated culture dish. Then, culture media treated with/without 2.0 IU/ml hCG or 10 ng/ml $IL-1{\beta}$. After incubation for 24 h, the co-incubated insert dishes were removed from CM-coated culture dish before embryo culture. Embryos at 48 h after in vitro fertilization (IVF) were cultured on the dish for 120 h with porcine zygote medium. We determined PTGS-2 expression in the ECs, VEGF protein in co-incubated CM with EC and observed cleavage rate and blastocyst development of embryos at 168 h after IVF. In result, expression of PTGS-2 was higher at co-incubated EC with hCG and $IL-1{\beta}$ groups than EC without hCG and $IL-1{\beta}$. The VEGF protein was detected at co-incubated CM with EC, EC treated with hCG and $IL-1{\beta}$ groups higher than CM group. Also, cleavage rate was no significantly difference among all group, however, blastocyst development was significantly higher in co-incubated CM with EC treated with hCG group than un-treated groups (p<0.05). Therefore, we suggest that novel embryo culture system using co-incubated collagen matrix gel with endometrial epithelial cells treated with $IL-1{\beta}$ is beneficial and useful for enhancing the production of porcine blastocysts in vitro.

• 대한지역사회영양학회지
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• 제13권3호
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• pp.346-358
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• 2008

The purpose of this study is to find differences in dietary patterns through menu analysis by economic status. The data was obtained from the 1998 and 2001 National Health and Nutrition Survey of Korea. The main variables were economic status, sex, and area by urbanization. The economic status was classified into low, middle, high, and top classes using a poverty line based on the 1998 and 2001 minimum standard cost of living. The areas were divided into metropolis, small city, and rural areas. The dishes of 3 meals were classified into 29 categories by cooking method. The most frequent pattern was "rice + soup + kimchi". The frequency of this Korean basic dietary pattern was the lowest in the top income class and metropolis areas, while the highest in the low income class and rural areas. The frequency of Korean recommended dietary pattern, that is, "rice + soup or stew + kimchi + side dish" was the highest in the top income class. The metropolis group preferred side dishes using meat and a cooking method that saved time, but the rural group preferred side dishes using vegetables and cooking methods that take a longer time. In comparison of dietary pattern between male and female by economic status, the higher economic status, the male's dietary patterns showed more side dishes than those of female. But the main side dish was kimchi in male low class. Consequently, the major dietary pattern in Korea is rice-style, though the western pattern is increasing in the top income class, especially in metropolis areas. Therefore, to make a better dietary pattern, we should develop and spread low-priced recipes of various side dishes and teach financial skills such as ability to make a food budget for lower income classes. Also, we should emphasize the importance of the balance between meat and vegetables and traditional diet and western diet for the top income class, especially in the metropolis areas.