• Parasites, Hosts and Diseases
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• 제56권2호
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• pp.189-194
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• 2018

To determine that Paragonimus sp. is actively transmitted in a tropical area of the Pacific region of Ecuador where human cases of pulmonary paragonimiasis have recently been documented, a total of 75 freshwater crabs were collected from 2 different streams in the Pedernales area of $Manab\acute{i}$ Province, Ecuador. All collected crabs were identified as Hypolobocera guayaquilensis based on morphological characteristics of the male gonopods. The hepatopancreas of each crab was examined by compressing it between 2 glass plates followed by observation under a stereomicroscope. Excysted Paragonimus metacercariae were detected in 39 (52.0%) crabs and their densities varied from 1 to 32 per infected crab. There was a positive relationship between crab size and metacercarial density. Sequences of the second internal transcribed spacer region of the ribosomal RNA gene of the Paragonimus metacercariae obtained in this study were identical to those of Paragonimus mexicanus deposited in the DDBJ/EMBL/GenBank database. Thus, the present study is the first to confirm that the crab species H. guayaquilensis is the second intermediate host of P. mexicanus in $Manab\acute{i}$ Province, Ecuador. Because this crab might be the possible source of human infections in this area, residents should pay attention to improper crab-eating habits related with a neglected parasitic disease, i.e., paragonimiasis.

• Parasites, Hosts and Diseases
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• 제55권3호
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• pp.333-336
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• 2017

Avian trichomoniasis caused by Trichomonas gallinae is a serious protozoan disease worldwide. The domestic pigeon (Columba livia domestica) is the main host for T. gallinae and plays an important role in the spread of the disease. Based on the internal transcribed spacers of nuclear ribosomal DNA of this parasite, a pair of primers (TgF2/TgR2) was designed and used to develop a PCR assay for the diagnosis of T. gallinae infection in domestic pigeons. This approach allowed the identification of T. gallinae, and no amplicons were produced when using DNA from other common avian pathogens. The minimum amount of DNA detectable by the specific PCR assay developed in this study was 15 pg. Clinical samples from Guangzhou, China, were examined using this PCR assay and a standard microscopy method, and their molecular characteristics were determined by phylogenetic analysis. All of the T. gallinae-positive samples detected by microscopic examination were also detected as positive by the PCR assay. Most of the samples identified as negative by microscopic examination were detected as T. gallinae positive by the PCR assay and were confirmed by sequencing. The positive samples of T. gallinae collected from Guangzhou, China, were identified as T. gallinae genotype B by sequencing and phylogenetic analyses, providing relevant data for studying the ecology and population genetic structures of trichomonads and for the prevention and control of the diseases they cause.

• 아시안잔디학회지
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• 제25권2호
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• pp.237-242
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• 2011

2011년 일본잔디학회 추계학술대회는 크게 일반 학술강연, 부회 및 소심포지움으로 나뉘어 총 55주제가 발표되었다. 일반 학술강연은 잔디, 병해충 및 잡초의 생리 생태분야, 병해 및 잡초방제분야 및 신규 잔디용 약제에 대한 약효특성 연구결과에 대해 발표되었다. 그중 잔디밭 최신 발생병해인 necrotic ring spot과 문제잡초인 파대가리, 새포아풀, 점나도나물에 대한 방제방안 및 UV를 이용한 살초스펙트럼을 조사한 연구발표가 있었다. 또한, 신규 등록 살균제 Furametpyr와 제초제인 S-metolachlor, Flucetosulfuron 및 Foramsulfuron의 방제효과에 대한 연구결과가 발표되었다. 부회에서는 골프장, 교정잔디, 공원녹지 및 지피식물 녹화의 4가지 주제와 잔디 유전자원 평가라는 소심포지움을 통해 다양한 정보교류가 있었다. 특히, 공원녹지 부회에서는 현재 일본의 사회적으로 가장 큰 이슈인 방사능오염물질 누출에 의한 공원, 교정 및 가정의 잔디밭의 방사능물질 오염 메카니즘과 오염물질을 효과적으로 제거하는 방법에 대하여 발표되었다.

• 생약학회지
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• 제49권2호
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• pp.182-187
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• 2018

Many plant derived phytochemicals have been considered as the main therapeutic strategy against Alzheimer's disease (AD). AD is a progressive neurodegenerative disorder, and the most predominant cause of dementia in the elderly. Cholinergic deficit, senile plaque/${\beta}$-amyloid ($A{\beta}$) peptide deposition and oxidative stress have been identified as three main pathogenic pathways which contribute to the progression of AD. We screened many different plant species for their effective use in both modern and traditional system of medicines. In this study, we tested that MeOH extract of the stem bark of Hopea chinensis (Merr.) Hand.-Mazz. (HCM) affects on the processing of Amyloid precursor portein (APP) from the APPswe over-expressing Neuro2a cell line. We showed that HCM reduced the secretion level of $A{\beta}42$ and $A{\beta}40$ in a dose dependent manner. We found that HCM increased over 1.5 folds of the secretion level of $sAPP{\alpha}$, a metabolite of ${\alpha}$-secretase. Furthermore, we found that HCM inhibited acetylcholinesterase activity in vitro. We suggest that the stem bark of Hopea chinensis may be a useful source to develop a therapeutics for AD.

• 보건행정학회지
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• 제10권4호
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• pp.1-19
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• 2000

We have witnessed several kinds of new discourses and practices in health and medicine since the 1970s, such as popular concerns with alternative or complementary medicine, inordinate attention to the promotion of 'healthy' living, rapid resurrection of traditional medicine and ecological management of health. Four structural and situational factors are discussed to underlie these new trends：(i) as 'crisis' in health care of the 1970s was translated into health care reform of the 1980s backed up by neo-liberal political philosophy, the state responsibility for nation's health is being transferred to the individual ；(ii) it resulted from the limits of biomedical paradigm in dealing with chronic diseases；(iii) medico-scientific knowledge of disease is transformed into the subjective discourses and technologies of health in postmodern society ； and (iv) it is deeply associated with the considerable increase in environmental risk perception of health and disease. There are some inherent countervailing forces in these new discourses and practices. First, while they derive from lifestyle-oriented behavioral change, medicalization of life and death is still consolidated in the new trends. Second, inasmuch as new tides are reliant upon science, they. are likely to be remote from techne that means not the practical application of theoretical knowing but a special form of practical knowing. Third, as new discourses and activities accomplished＇in the name of health＇increasingly occupy important strategies in forming the self-identity, they serve as moral apparatus which involves prescriptions about how we should live our lives and conduct our bodies, both individually and collectively. Therefore, two points are suggested to consider seriously whether these streams will succeed in improving the‘healthy’living of all the people. Instead of limiting tile perspective to medicine, healing and health care, a new matrix that interweave welfare, ecology and labor along with them is timely needed for enhancing the health for all. In addition, as the World Health Report fm strongly shows, inequality in health heavily depends upon socio-economic development of a society, and it is not the richest countries that have the best health status, but those that have the smallest income differences between rich and poor.

• 대한지리학회지
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• 제48권2호
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• pp.218-238
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• 2013

한국 지리학계에서 보건의료에 대한 지리학적 연구가 증가되고 있는 양상에 비추어 볼 때, 의료지리학이 개념적으로 어떻게 발달해왔는지에 대한 역사적인 인식이 요청된다. 이 글의 목적은 의료지리학의 개념적 역사를 탐구하고 향후 의료지리학의 역사적 전망을 제시하는 데 있다. 근대 의료지리학은 유럽이 열대 식민지를 통치하는 과정에서 필연적으로 직면하게 되었던 열대 질병을 통제하는 과정에서 의료지형학의 이름으로 형성되었다. 영국에서는 환경위생 개혁의 명분으로, 프랑스에서는 문명화 사명의 구호 아래, 독일에서는 지리의학의 개념으로 각각 발달해왔던 의료지리학은 20세기에 크게 질병 생태학과 의료체계의 두 가지 흐름으로 발달되었다. 본 논문은 질병의 지도에 함축된 권력의 의미를 강조하면서, 의료지리학, 보건지리학, 건강지리학의 공통 지점과 서로 다른 지향점을 제시하였다는 데 의의가 있다.

• 대한영양사협회학술지
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• 제17권1호
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• pp.58-71
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• 2011

The purpose of this study was to investigate the perceptions of an unbalanced diet by elementary school students using a qualitative approach to nutrition counseling. To achieve the purpose of this study, client-centered counseling was performed over one semester with four volunteer children in the fifth grade who admitted eating an unbalanced diet. Their perceptions of an unbalanced diet were examined while focusing on cause. The contents of the counseling sessions were recorded and their eating habits observed. The proposed perceptions of the clients of an unbalanced diet were as follows: regarding the causes of an unbalanced diet, there may have been a certain negative event or memory related to food as well as an individual disease or history of disease; regarding the meaning of an unbalanced diet, the clients had two kinds of time concepts (formal one and informal one) and defined the meaning of an unbalanced diet as a matter of being able to eat proper amounts of food within an inter-meal time; and regarding the results of an unbalanced diet, they based their results on the approval of their parents and teachers. Feeding at school was used as the background of this study, which examined the meanings and results of an unbalanced diet, and there existed a correlation between the meanings and the results.

• Parasites, Hosts and Diseases
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• 제56권6호
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• pp.619-623
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• 2018

Bovine coccidiosis is one of the most important parasitic diseases affecting calf productivity. Here, we investigated the prevalence of Eimeria spp. in pre-weaned native Korean calves and determined the correlation between diarrhea and Eimeria spp. Fecal samples were collected from individual calves (288 normal and 191 diarrheic) in 6 different farms. Of the 479 samples, Eimeria oocysts were detected in 124 calves (25.9%). Five Eimeria spp. were identified; E. zuernii (18.8%) was the most prevalent, followed by E. auburnensis (12.5%), E. bovis (7.5%), E. subspherica (5.8%), and E. bukidnonensis (1.0%). A significant correlation was observed between diarrhea and mixed infection with more than 2 Eimeria spp. (odds ratio [OR]=2.21; 95% confidence interval [CI]: 1.09-4.49; P=0.03) compared to single infection (OR=1.29; 95% CI: 0.77-2.15; P=0.33). Of the 5 Eimeria spp. identified, E. subspherica (95% CI: 1.24-5.61; P=0.01) and E. bukidnonensis (95% CI: 825.08-1,134.25; P=0.00) strongly increased the risk of diarrhea by 2.64-fold and 967.39-fold, respectively, compared to other species. Moreover, mixed infection with E. auburnensis and E. bukidnonensis was significantly associated with diarrhea (OR=2,388.48; 95% CI: 1,009.71-5,650.00; P<0.00) in pre-weaned native Korean calves. This is the first report to demonstrate the importance of E. bukidnonensis associated with diarrhea in pre-weaned native Korean calves. Further epidemiological studies should investigate the prevalence of E. bukidnonensis and the association between E. bukidnonensis and diarrhea.

• 한국환경농학회지
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• 제38권3호
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• pp.185-196
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• 2019

BACKGROUND: This study investigated the effects of rice genetically modified to be resistant against rice blast and rice bacterial blight on the soil microbial community. A comparative analysis of the effects of rice genetically modified rice choline kinase (OsCK1) gene for disease resistance (GM rice) and the Nakdong parental cultivar (non-GM rice) on the soil microbial community at each stage was conducted using rhizosphere soil of the OsCK1 and Nakdong rice. METHODS AND RESULTS: The soil chemistry at each growth stage and the bacterial and fungal population densities were analyzed. Soil DNA was extracted from the samples, and the microbial community structures of the two soils were analyzed by pyrosequencing. No significant differences were observed in the soil chemistry and microbial population density between the two soils. The taxonomic analysis showed that Chloroflexi, Proteobacteria, Firmicutes, Actinobacteria, and Acidobacteria were present in all soils as the major phyla. Although the source tracking analysis per phylogenetic rank revealed that there were differences in the bacteria between the GM and non-GM soil as well as among the cultivation stages, the GM and non-GM soil were grouped according to the growth stages in the UPGMA dendrogram analysis. CONCLUSION: The difference in bacterial distributions between Nakdong and OsCK1 rice soils at each phylogenetic level detected in microbial community analysis by pyrosequencing may be due to the genetic modification done on GM rice or due to heterogeneity of the soil environment. In order to clarify this, it is necessary to analyze changes in root exudates along with the expression of transgene. A more detailed study involving additional multilateral soil analyses is required.

• Journal of Ecology and Environment
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• 제44권4호
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• pp.222-228
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• 2020

Background: Parthenium hysterophorus is a noxious invasive weed in tropical and subtropical regions of the world, including Nepal. Among 11 species of biological control agents released to control P. hysterophorus in Ausrtalia, winter rust Puccina abrupta var. partheniicola arrived fortuitously and has established in Kathmandu Valley, Nepal, nearly a decade back. However, the prevalence and effectiveness of this rust as a biological control agent in Kathmandu remain unknown. To address this knowledge gap, a roadside survey was done at an interval of 2 ± 0.25 km in Kathmandu Valley to assess the P. abrupta var. partheniicola incidence and its impacts on P. hysterophorus. Infested individuals of P. hysterophorus were further divided into four severity classes (very low, low, medium, and high), and rust incidence was calculated. The impact of the winter rust on the growth of P. hysterophorus was assessed by comparing biomass and seed output of infested and non-infested individuals. Results: Among 81 locations where P. hysterophorus was present in the Kathmandu Valley, winter rust infestation was observed at 98% locations. At some locations within Kathmandu Valley such as Tinkune, Kirtipur, Chabahil, Buddha Chowk, and Dhobighat, the impacts of the rust on P. hysterophorus were medium to high. Aboveground biomass and seed output of P. hysterophorus were reduced by 47% and 73%, respectively, due to winter rust infestation. The study indicates that winter rust incidence is widespread in Kathmandu Valley with very low to high levels of damages to P. hysterophorus depending on the localities. Conclusion: The rust has, therefore, a potential to reduce the growth performance of P. hysterophorus, and it can be used as a component of integrated management of P. hysterophorus by introducing to other suitable areas in Nepal.