• The Korean Journal of Physiology and Pharmacology
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• 제20권2호
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• pp.161-168
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• 2016

Abnormal localization of tumor suppressor proteins is a common feature of renal cancer. Nuclear export of these tumor suppressor proteins is mediated by chromosome region maintenance-1 (CRM1). Here, we investigated the antitumor effects of a novel reversible inhibitor of CRM1 on renal cancer cells. We found that S109 inhibits the CRM1-mediated nuclear export of RanBP1 and reduces protein levels of CRM1. Furthermore, the inhibitory effects of S109 on CRM1 is reversible. Our data demonstrated that S109 significantly inhibits proliferation and colony formation of renal cancer cells. Cell cycle assay showed that S109 induced G1-phase arrest, followed by the reduction of Cyclin D1 and increased expression of p53 and p21. We also found that S109 induces nuclear accumulation of tumor suppressor proteins, Foxo1 and p27. Most importantly, mutation of CRM1 at Cys528 position abolished the effects of S109. Taken together, our results indicate that CRM1 is a therapeutic target in renal cancer and the novel reversible CRM1 inhibitor S109 can act as a promising candidate for renal cancer therapy.

• The Korean Journal of Physiology and Pharmacology
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• 제14권5호
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• pp.299-304
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• 2010

Losartan is a selective angiotensin II (Ang II) type 1 ($AT_1$) receptor antagonist which inhibits vascular smooth muscle cells (VSMCs) contraction and proliferation. We hypothesized that losartan may prevent cell proliferation by activating AMP-activated protein kinase (AMPK) in VSMCs. VSMCs were treated with various concentrations of losartan. AMPK activation was measured by Western blot analysis and cell proliferation was measured by MTT assay and flowcytometry. Losartan dose- and time-dependently increased the phosphorylation of AMPK and its downstream target, acetyl-CoA carboxylase (ACC) in VSMCs. Losartan also significantly decreased the Ang II- or 15% FBS-induced VSMC proliferation by inhibiting the expression of cell cycle associated proteins, such as p-Rb, cyclin D, and cyclin E. Compound C, a specific inhibitor of AMPK, or AMPK siRNA blocked the losartan-induced inhibition of cell proliferation and the $G_0/G_1$ cell cycle arrest. These data suggest that losartan-induced AMPK activation might attenuate Ang II-induced VSMC proliferation through the inhibition of cell cycle progression.

• 대한지역사회영양학회지
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• 제5권4호
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• pp.697-708
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• 2000

The purpose of this study is to analyze the contents of the questionnaires used in dietary surveys and to evaluate each item in relation to the item construction strategy. Articles of which the contents were related to food, nutrition, diet, dietary behavior, and related areas. Published from 1997 to 1999 were searched fir and a total of 121 questionnaires were collected and analyzed. The questions in the questionnaires were classified into related areas and sub-areas. Among the keywords in the title of the articles, the term 'nutritional status'(or 'dietary intake status') was most frequently used. The terms such as dietary status, obesity, health, food habit, and dietary behavior were also frequency used. Major topics of the items in the questionnaires varied according to the life cycle of the subjects of the study. The topics most frequently asked in each lift cycle were as follows : overeating, snack, and food preference for preschool- and school-aged children ; anthropometry, weight control, and snack for middle and highschool students : meal skipping, smoking, and drinking for college students : disease, smoking, drinking, and exercise for adults : and smoking, drinking, disease and perceived health for the elderly. Inappropriate questions with complicated language, typographic and grammatic errors, unnecessary words, and negative questions were found. Therefore, care should be taken to construct each question so as to avoid possible misinterpretation. Also, a standardized questionnaire be developed for survey researchers.

• 대한한방부인과학회지
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• 제18권1호
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• pp.156-168
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• 2005

Objective : The purpose of this study is to identify the clinical effects of Chiljehyangbuhwan in the treatment of dysmenorrhea patients. Methods : We studied fifty patients who visited Kyung Hee University Oriental Medical Center from July, 2004 to August, 2004. Women with organic disease such as ut. myoma, ov. cyst and pelvic inflammatory disease were excluded from this study. We treated them with Chiljehyangbuhwan for one menstrual cycle. The severity of dysmenorrhea were measured by MVRS (Multidimensional Verbal Rating scale), VRS (Verbal Rating scale) and VAS (Visual Analog Scale). Results : Chiljehyangbuhwan significantly decreased the severity of dysmenorrhea. Furthermore Chiljehyangbuhwan had an effect on dysmenorrhea for next menstrual cycle without taking. Chiljehyangbuhwan did not show hepatic and renal virulence. Conclusion : This study shows that Chiljehyangbuhwan has remarkable effects on dysmenorrhea patients and that effects continue to next menstrual cycle without taking medicine.

• 셀메드
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• 제12권3호
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• pp.10.1-10.12
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• 2022

Objective: The objective of the study was to evaluate the effect of Sharbat Afsantin in Polycystic Ovarian Disease. Methods: An open observational study was carried out in the Department of Ilmul Qabalat wa Amraze Niswan. Diagnosed cases(n=30) of PCOD were included in the study. Patients with thyroid dysfunction, systemic diseases, on hormonal treatment in last three months, pregnancy and lactation were excluded. Research drug (Sharbat Afsantin) was administered orally in a dose of 25ml twice daily for 15 days/cycle for three consecutive cycles. Outcome measures were, changes in subjective parameters (duration of cycle, duration and amount of flow and weight reduction) and objective parameters {pictorial blood loss assessment chart (PBAC) score, basal metabolic index (BMI), modified Ferriman Gallwey (mFG) score, acanthosis nigricans scale and pelvic ultrasonography}. Data were analyzed using paired Student 't' test. Results: Changes in duration of cycle, duration and amount of flow were achieved in 83.3%, 50% and 40% patients respectively and weight reduction in 30% patients. Changes in PBAC score and BMI were achieved in 50% and 30% patients respectively and 30% patients showed normal findings on pelvic ultrasonography. Conclusion: Sharbat Afsantin can be used as an alternate remedy in PCOD patients, as it has significant effect to regularize menstruation by reduction in BMI and probably by improving insulin resistance in PCOD. No adverse effect of Sharbat Afsantin was noted during the trial.

• 대한약침학회지
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• 제11권2호
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• pp.13-19
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• 2008

Objectives To investigate the therapeutic effects of intravenous cultivated wild ginseng(Panax ginseng C.A. Meyer) pharmacopuncture(CWGP) in treating patients with non-small cell lung cancer(NSCLC). Design Prospective case series. Setting This study was conducted at the East-West Cancer Center of Dunsan Oriental Hospital, Daejeon University. Patients Two non-small cell lung cancer patients. Intervention Two non-small cell lung cancer patients were injected CWGP(20mL/day) mixed with 0.9% normal saline(100mL) intravenously. Each patient received a total of 16 and 9 cycles, respectively. One cycle is composed of 14 days. Outcome Measures The effect of intravenous CWGP was measured by scanning with computed tomography(CT) after every 2 cycle and Positron emission tomography- computed tomography(PET/CT) after every 6 cycles. Response and progression was evaluated using the Response Evaluation Criteria in Solid Tumors(RECIST) Committee classification of complete response(CR), partial response(PR), progressive disease(PD) and stable disease(SD). Results They were treated with intravenous CWGP for 8 and 5 months respectively. time later, each tumor remains stable disease(SD). Conclusion These cases may give us a possibility that intravenous CWGP offers potential benefits for non-small cell lung cancer patients.

• Journal of Medicine and Life Science
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• 제20권3호
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• pp.107-114
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• 2023

Epidemiological control of coronavirus disease 2019 (COVID-19) is needed to estimate the infection period of confirmed cases and identify potential cases. The present study, targeting confirmed cases for which the time of COVID-19 symptom onset was disclosed, aimed to investigate the relationship between intervals (day) from symptom onset to testing the cycle threshold (CT) values of real-time reverse transcription-polymerase chain reaction. Of the COVID-19 confirmed cases, those for which the date of suspected symptom onset in the epidemiological investigation was specifically disclosed were included in this study. Interval was defined as the number of days from symptom onset (as disclosed by the patient) to specimen collection for testing. A locally weighted regression smoothing (LOWESS) curve was applied, with intervals as explanatory variables and CT values (CTR for RdRp gene and CTE for E gene) as outcome variables. After finding its non-linear relationship, a polynomial regression model was applied to estimate the 95% confidence interval values of CTR and CTE by interval. The application of LOWESS in 331 patients identified a U-shaped curve relationship between the CTR and CTE values according to the number of interval days, and both CTR and CTE satisfied the quadratic model for interval days. Active application of these results to epidemiological investigations would minimize the chance of failing to identify individuals who are in contact with COVID-19 confirmed cases, thereby reducing the potential transmission of the virus to local communities.

• The Plant Pathology Journal
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• 제23권1호
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• pp.3-6
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• 2007

Chickpea wilt caused by Fusarium oxysporum f. sp. ciceris(FOC) is the most destructive disease in India. It is seed-borne as well as soil-borne pathogen. The role of seed-borne FOC in introducing and establishing wilt in FOC free soils is unknown. Using seeds of FOC infected chickpea cultivar K 850, we provided an evidence of establishing wilt disease in the FOC free soils within three crop cycles or seasons. In the first cycle, typical wilt symptoms were observed in 24 pots in 41 days after sowing. These 24 pots were used for second and third cycles without changing the soil. These 24 pots were sown with seeds collected from healthy plants of a susceptible cultivar JG 62, one seed per pot and development of wilt symptom was recorded. Wilt symptoms appeared in all the pots 26 days after sowing in second cycle and in 16 days after sowing in third cycle. On selective medium, all of the wilted plants yielded FOC in all the three cycles indicating that the mortality was due to wilt. FOC propagules on selective medium were 172, 1197, and 2280 $g^{-1}$ soil at the end of the first, second, and third cycles, respectively. These studies indicated that Fusarium wilt of chickpea is seed-borne and seeds harvested from wilted plants when mixed with healthy seeds can carry the wilt fungus to new areas and can establish the disease in the soil to economic threshold levels within three seasons.

• IMMUNE NETWORK
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• 제11권5호
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• pp.227-244
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• 2011

MicroRNAs (miRNAs) are small noncoding RNA molecules that negatively regulate gene expression via degradation or translational repression of their target messenger RNAs (mRNAs). Recent studies have clearly demonstrated that miRNAs play critical roles in several biologic processes, including cell cycle, differentiation, cell development, cell growth, and apoptosis and that miRNAs are highly expressed in regulatory T (Treg) cells and a wide range of miRNAs are involved in the regulation of immunity and in the prevention of autoimmunity. It has been increasingly reported that miRNAs are associated with various human diseases like autoimmune disease, skin disease, neurological disease and psychiatric disease. Recently, the identification of miRNAs in skin has added a new dimension in the regulatory network and attracted significant interest in this novel layer of gene regulation. Although miRNA research in the field of dermatology is still relatively new, miRNAs have been the subject of much dermatological interest in skin morphogenesis and in regulating angiogenesis. In addition, miRNAs are moving rapidly center stage as key regulators of neuronal development and function in addition to important contributions to neurodegenerative disorder. Moreover, there is now compelling evidence that dysregulation of miRNA networks is implicated in the development and onset of human neruodegenerative diseases, such as Alzheimer's disease, Parkinson's disease, Huntington's disease, Tourette's syndrome, Down syndrome, depression and schizophrenia. In this review, I briefly summarize the current studies about the roles of miRNAs in various autoimmune diseases, skin diseases, psychoneurological disorders and mental stress.

• 디지털융복합연구
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• 제19권4호
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• pp.205-215
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• 2021

본 연구는 생애주기별 건강증진 및 만성질환 관리에 대한 교육요구도를 조사하여 웰에이징을 위한 건강증진 및 만성질환 관리의 교육 프로그램 및 교육안을 구축하는데 기초자료를 제공하고자 시행되었다. 서술적 상관관계 조사 연구로 한국갤럽에 등록된 만 19세 이상 성인 330명을 대상으로 구조화된 설문지를 통해 수집하고 분석하였다. 연구 결과 주관적 건강 상태는 3.11점(SD=.72), 건강증진행위는 2.27점(SD=.47), 건강 관련 삶의 질은 0.92점(SD=.10)이었으며, 건강 관련 삶의 질은 주관적 건강 상태(r=.472, p<.001), 건강증진행위(r=.122, p<.026)와 양의 상관관계가 있는 것으로 나타났다. 건강증진 및 만성질환 관리에 대한 교육요구도 및 내용의 우선순위는 만성질환 관리에 대한 부분이 가장 높았고, 생애주기에 따라 유의한 차이가 있었으며(p=0.001), 장년기가 청년기보다 지식 정도, 교육의 필요도에 대한 인식 및 교육 참여 의향이 높은 것으로 나타났다. 본 연구 결과를 통해 웰에이징을 위한 건강증진 및 만성질환 관리 교육 프로그램 개발 시 생애주기별에 따라 세분화 된 종합적인 맞춤형 프로그램을 개발할 필요가 있다.