• 대한예방한의학회지
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• 제26권3호
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• pp.73-86
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• 2022

Objectives : The purpose of this study was to investigate the satisfaction of students who participated in a role play using the syndrome differentiation CPX (Clinical Performance Examination) scenario in Korean Medicine Classics class, and to find out whether the results were correlated with self-assessment of treatment skills and subject achievement. Methods : In the Korean Medicine Classics class in the first semester of 2022, 44 first-year students in the Department of Korean Medicine completed theoretical education and formative evaluation on the subject of internal damage fever, and then role-played using CPX scenarios. Among them, 41 students consented to the study. Students who agreed to the study answered the satisfaction questionnaire consisting of 13 questions in 2 areas on learning methods and effects, and self-evaluation was conducted according to the evaluation items of medical technology consisting of 23 questions. Satisfaction and self-assessment results were analyzed for correlation, and additionally, correlation with Korean Medicine Classics subject achievement was also analyzed. Results : The result of student satisfaction with the role play was 4.87±0.06 while the result of student self-assessment of clinical skill was 4.73±0.16. The student satisfaction with the role play showed statisticallysignificant correlations with the self-assessment of clinical skill and quiz score of Korean Medical Classics while it did not show correlations with the total score of Korean Medical Classics and paper test. Also, the self-assessment of clinical skill did not show correlations with the total score of Korean Medical Classics, paper test, and quiz. Conclusions : Even though the students who performed the role play using the syndrome differentiation based CPX scenario highly assessed their clinical skill and showed high satisfaction, it did not show significant correlation with the achievement of Korean Medical Classics.

• 아동학회지
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• 제20권4호
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• pp.195-211
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• 1999

This study investigated developmental trends and sex differences in the relation between children's self-esteem and relevant variables by proposing and testing the causal model. The 763 children who participated in the study were 3rd, 5th, and 7th grade students. Major findings were that physical appearance was the most powerful determinant of self-esteem. Students with high self-esteem were more learning oriented, used more motivational behaviors and had higher academic achievement. The findings from this analysis of the causal model revealed remarkable developmental differentiation and stability.

• International Journal of Stem Cells
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• 제17권1호
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• pp.59-69
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• 2024

Human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs), induced pluripotent stem cells, and somatic cell nuclear transfer (SCNT)-hESCs can permanently self-renew while maintaining their capacity to differentiate into any type of somatic cells, thereby serving as an important cell source for cell therapy. However, there are persistent challenges in the application of hPSCs in clinical trials, where one of the most significant is graft rejection by the patient immune system in response to human leukocyte antigen (HLA) mismatch when transplants are obtained from an allogeneic (non-self) cell source. Homozygous SCNT-hESCs (homo-SCNT-hESCs) were used to simplify the clinical application and to reduce HLA mismatch. Here, we present a xeno-free protocol that confirms the efficient generation of neural precursor cells in hPSCs and also the differentiation of dopaminergic neurons. Additionally, there was no difference when comparing the HLA expression patterns of hESC, homo-SCNT-hESCs and hetero-SCNT-hESCs. We propose that there are no differences in the differentiation capacity and HLA expression among hPSCs that can be cultured in vitro. Thus, it is expected that homo-SCNT-hESCs will possess a wider range of applications when transplanted with neural precursor cells in the context of clinical trials.

• Journal of Yeungnam Medical Science
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• 제26권1호
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• pp.1-14
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• 2009

Human bone marrow-derived mesenchymal stem cells (MSCs) are a rare population of undifferentiated cells that have the capacity of self renewal and the ability to differentiate into mesodermal phenotypes, including osteocytes, chondrocytes, and adipocytes in vitro. Recently, MSCs have been shown to reside within the connective tissue of most organs, and their surface phenotype has been well analyzed. Many reports showed that transplanted MSCs enhanced regeneration as well as functional improvement of damaged organs and tissues. The wide differentiation plasticity of MSCs was expected to contribute to their demonstrated efficacy in a wide variety of experimental animal models and in human clinical trials. However, new findings suggest that the ability of MSCs to alter the tissue microenvironment via secretion of soluble factors may contribute more significantly than their capacity for differentiation in tissue repair. This review describes what is known about the cellular characteristics and differentiation potential of MSCs, which represent a promising stem cell population for further applications in regenerative medicine.

• 한국생활과학회지
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• 제22권4호
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• pp.559-574
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• 2013

The purpose of this study was to develop the couple relationship improvement program for college students and to examine the effects of the program based on the family of origin and attachment. Does based on Bowen's theory of differentiation of self, adult attachment, group counseling for this and establishes program goals according to systematic group counseling program development model and composed program. In order to attain like this research objective, six male and female couples attending colleges were divided into the participating group and comparison group; the students were from H University in Daejeon, Korea, and they had the experience of participating in the comprehensive group program. The changes shown by the couples were studied, and the cases were analyzed by examining the minutes and reports, which supplemented the quantitative analysis of the effectiveness of the group counseling program. The analysis of the effectiveness of the program showed the following. The case showed that goals of the individuals and couples, enhancing the understanding of self and partners by understanding the self-differentiation and adult attachment while having a positive impact on understanding, acceptance, disclosure of self partner and couple relationship based on the social support of the cognitive emotion regulation strategies.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.80-104
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• 1996

The development of routine techniques for the isolation and in vitro maintenance of conducting airway epithelial cells in a differentiated state provides an ideal model to study the factors involved in the regulation of the expression of mucocilicary differentiation. Several key factors and conditions have been identified. These factors and conditions include the use of biphasic culture technique to achieve mucociliary differentiation and the use of such stimulators, the thickness of collagen gel substratum, the calcium level, and vitamin A, and such inhibitors, the growth factors EGF and insulin, and steroid hormones, for mucous cell differentiation. Using the defined culture medium, the life cycle of the mucous cell population in vitro was investigated. It was demonstrated that the majority of the mucous cell population in primary cultures is not involved in DNA replication. However, the mucous cell type is capable of self-renewal in culture and this reproduction is vitamin A dependent. furthermore, differentiation from non-mucous cell type to mucous cell type can be demonstrated by adding back a positive regulator such as vitamin A to the “starved” culture. Cell kinetics data suggest that vitamin A-dependent mucous cell differentiation in culture is a DNA replication-independent process and the process is inhibited by TGF-${\beta}$1.

• 한국동물생명공학회지
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• 제34권1호
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• pp.2-9
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• 2019

Because mesenchymal stem cells (MSCs) maintain distinct capacities with respect to self-renewal, differentiation ability and immunomodulatory function, they have been highly considered as the therapeutic agents for cell-based clinical application. Of particular, differentiation condition alters characteristics of MSCs, including cellular morphology, expression of gene/protein and cell surface molecule, immunological property and apoptosis. However, the previous results for differentiation-related apoptosis in MSCs have still remained controversial due to varied outcomes. Therefore, the present study aimed to disclose periodical alterations of pro- and anti-apoptosis in MSCs under differentiation inductions. The human dental pulp-derived MSCs (DP-MSCs) were differentiated into adipocytes and osteoblasts during early (1 week), middle (2 weeks) and late (3 weeks) stages, and were investigated on their apoptosis-related changes by Annexin V assay, qRT-PCR and western blotting. The ratio of apoptotic cell population was significantly (p < 0.05) elevated during the early to middle stages of differentiations but recovered up to the similar level of undifferentiated state at the late stage of differentiation. In the expression of mRNA and protein, whereas expressions of pro-apoptosis-related makers (BAX and BAK) were not altered in any kind and duration of differentiation inductions, anti-apoptosis marker (BCL2) was significantly (p < 0.05) elevated even at the early stage of differentiations. The recovery of apoptotic cell population at the late stage of differentiation is expected to be associated with the response by elevation of anti-apoptotic molecules. The present study may contribute on understanding for cellular mechanism in differentiation of MSCs and provide background data in clinical application of MSCs in the animal biotechnology to develop effective and safe therapeutic strategy.

• 대한가정학회지
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• 제41권3호
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• pp.31-44
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• 2003

The purpose of this study is to investigate the difference of maternal parenting stress through early childhood between urban(Seoul and Incheon) and rural area(Iksan and Kimpo). The subjects were 286 unemployed mothers who have less than 5-year-old children. The statistics used for this data were frequency, percentile, t-test, ANOVA, and multiple regression. The finding from the present study certainly suggested that mother's level of self-differentiation, marital satisfaction, father's parenting support turned out to a significant variables on maternal parenting stress. Results of the regression analysis that the etiologic model of this study was more proper to urban than rural area, and father's parenting support is the most effective variable in predicting maternal parenting stress in both area. Implications based on this study are as follows ; parenting education programs to encourage for father's parenting support with be developed, and various empirical studies about maternal parenting stress in with area will be needed.

• 한국IT서비스학회지
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• 제22권5호
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• pp.51-70
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• 2023

The Personal Information Protection Commission has been promoting the adoption of co-regulation to replace/improve the existing self-regulation programs since 2022. While the Commission's co-regulation framework has received positive feedback for its contribution to address the regulatory issues, it has also faced criticism for being seen as "co-regulation in appearance only without any real differentiation from existing self-regulation." This study aims to examine the case of industry-specific co-regulation proposed by the Personal Information Protection Commission, with the objectives of 1) determining whether their approach can be categorized as a type of co-regulation with differentiation from the existing self-regulation programs, and 2) proposing improvements for successful regulation. The 'co-regulation' of the Commission can be classified as a form of co-regulation that differs with traditional self-regulation, as it involves government and private organizations collaborating to establish self regulatory codes reflecting industry-specific characteristics as well as provides interest groups with incentives to comply with the codes. The co-regulation framework is evaluated to some extent as successful, but there is still room for improvement in three major aspects. (1) When selecting the areas for co-regulation, a focus should be placed on areas where technological changes are rapid, and government regulations should be applied in areas where they are not. (2) It is necessary to enhance the expertise of regulatory agencies, and (3) ensuring the democratic nature of regulation, such as encouraging the participation of civil organizations, is necessary.

• 대한치과교정학회지
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• 제42권5호
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• pp.249-254
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• 2012

Objective: To investigate the stem cell-like characteristics of human periodontal ligament (PDL) stromal cells outgrown from orthodontically extracted premolars and to evaluate the potential for myogenic differentiation. Methods: PDL stromal cells were obtained from extracted premolars by using the outgrowth method. Cell morphological features, self-replication capability, and the presence of cell-surface markers, along with osteogenic, adipogenic, and chondrogenic differentiation, were confirmed. In addition, myogenic differentiation was induced by the use of 5-aza-2'-deoxycytidine (5-Aza) for DNA demethylation. Results: PDL stromal cells showed growth patterns and morphological features similar to those of fibroblasts. In contrast, the proliferation rates of premolar PDL stromal cells were similar to those of bone marrow and adipogenic stem cells. PDL stromal cells expressed surface markers of human mesenchymal stem cells (i.e., CD90 and CD105), but not those of hematopoietic stem cells (i.e., CD31 and CD34). PDL stromal cells were differentiated into osteogenic, adipogenic, and chondrogenic lineages. Myotube structures were induced in PDL stromal cells after 5-Aza pretreatment, but not in the absence of 5-Aza pretreatment. Conclusions: PDL stromal cells isolated from extracted premolars can potentially be a good source of postnatal stem cells for oromaxillofacial regeneration in bone and muscle.