International Journal of Stem Cells

Korean Society for Stem Cell Research (한국줄기세포학회)
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Expression of Major Histocompatibility Complex during Neuronal Differentiation of Somatic Cell Nuclear Transfer-Human Embryonic Stem Cells

  • Jin Saem Lee (Graduate School of Biomedical Science and Engineering, Hanyang University) ;
  • Jeoung Eun Lee (CHA Advanced Research Institute, CHA University) ;
  • Shin-Hye Yu (Graduate School of Biomedical Science and Engineering, Hanyang University) ;
  • Taehoon Chun (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Mi-Yoon Chang (Department of Premedicine, College of Medicine, Hanyang University) ;
  • Dong Ryul Lee (CHA Advanced Research Institute, CHA University) ;
  • Chang-Hwan Park (Graduate School of Biomedical Science and Engineering, Hanyang University)
  • Received : 2023.03.27
  • Accepted : 2023.09.08
  • Published : 2024.02.28
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Abstract

Human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs), induced pluripotent stem cells, and somatic cell nuclear transfer (SCNT)-hESCs can permanently self-renew while maintaining their capacity to differentiate into any type of somatic cells, thereby serving as an important cell source for cell therapy. However, there are persistent challenges in the application of hPSCs in clinical trials, where one of the most significant is graft rejection by the patient immune system in response to human leukocyte antigen (HLA) mismatch when transplants are obtained from an allogeneic (non-self) cell source. Homozygous SCNT-hESCs (homo-SCNT-hESCs) were used to simplify the clinical application and to reduce HLA mismatch. Here, we present a xeno-free protocol that confirms the efficient generation of neural precursor cells in hPSCs and also the differentiation of dopaminergic neurons. Additionally, there was no difference when comparing the HLA expression patterns of hESC, homo-SCNT-hESCs and hetero-SCNT-hESCs. We propose that there are no differences in the differentiation capacity and HLA expression among hPSCs that can be cultured in vitro. Thus, it is expected that homo-SCNT-hESCs will possess a wider range of applications when transplanted with neural precursor cells in the context of clinical trials.

Keywords

Acknowledgement

This research was supported by grants from the Basic Science Research Program (NRF-2019R1A2C2005681) and the Bio & Medical Technology Development Program (NRF-2022M3A9I2082319) funded by the Ministry of Science and ICT, Republic of Korea.

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