• 제목/요약/키워드: differentiated approach

검색결과 255건 처리시간 0.028초

젤 전기영동 및 액체 크로마토그래피 분리 방법을 이용하여 지방 세포로부터 분비되는 단백질들에 대한 프로테오믹스 연구 방법 (Intensive Proteomic Approach to Identify Secreted Peptides/Proteins from 3T3-L1 Adipocytes using Gel Electrophoresis and Liquid Chromatograph Separation Methods)

  • 황현호;백문창
    • 약학회지
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    • 제55권3호
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    • pp.203-212
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    • 2011
  • Adipocytes have been known to secrete a number of important proteins called adipokines with roles in energy metabolism, reproduction, cardiovascular function and immunity. In this study we have attempted to identify intensively secretory proteins from 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into mature adipocytes and then the cells were left in serum-free medium. The supernatant was filtrated and dialyzed. Lyophilized secretome was fractionated by two different methods, 1-D SDS PAGE and RP-FPLC. The tryptic peptides from the gel slices and the FPLC fractions were analyzed by nanoLC/ESI-MS/MS. We identified a total of 303 identical proteins from two methods, 251 proteins from 1-D gel and 184 proteins from RP-FPLC. 86 of them were listed as a secretory protein Finally, we identified many known or unknown secreted proteins existed in the low level including adiponectin, angiotensinogen, bone morphogenetic protein-1 (BMP-1), macrophage migration inhibitory factor (MIF), insulin like growth factor-II (IGF-II), interleukin-6 (IL-6), follistatin-related protein-1, minecan, and resistin. The existence of some of secreted proteins has been confirmed in RNA level. This proteomic experiment is useful for the intensive screening of secretory proteins in many kinds of other cells.

웨딩플래너 선택속성에 관한 에스노그라피적 연구 (Ethnographic study of the selection attributes for wedding planner)

  • 김하정;유지헌
    • 복식문화연구
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    • 제26권2호
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    • pp.217-232
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    • 2018
  • As the types of wedding ceremonies become diversified, and consumers' needs become more selective, greater importance is given to wedding planners' roles in the wedding industry, and consumers require differentiated types of wedding service. As a preliminary qualitative study in this field, an ethnographic study was conducted to identify factors valued by consumers when selecting a wedding planner in the wedding planner market, which has rapidly grown in recent years. An in-depth interview was performed with eight participants in terms of wedding planner utilization type and consumers' understanding of wedding preparation with a wedding planner. The collected data were analyzed through taxonomy, component analysis and decision table analysis; for validation, professional wedding planners were asked to review the items valued by consumers in selecting a wedding planner. Four factors - expense characteristic, wedding planners, wedding consulting companies, and customer characteristics - were identified, along with eight sub-factors (capability, service attitude, technical communication, personal factors, wedding planner encounter paths, spouse's satisfaction status, and companies). Out of these, price (within budget), wedding planners' capability, wedding planners' service attitude are expected to be meaningful in further research because they were found to be attributes commonly valued by every respondent. This study is significant in that it has made a new approach to understanding wedding planner selection attributes through ethnographic research and identified new wedding planner selection attributes.

Policy-Based QoS Management for SLA-Driven Adaptive Routing

  • Katsikogiannis, George;Mitropoulos, Sarandis;Douligeris, Christos
    • Journal of Communications and Networks
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    • 제15권3호
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    • pp.301-311
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    • 2013
  • This paper proposes a policy-based quality of service (QoS) management framework for adaptive routing decisions. We present an approach considering interior gateway protocol (IGP) for path discovery mechanisms and QoS-aware policies for configuring the network elements. The integration of the aforementioned modules into this policy-based network management (PBNM) system is demonstrated by conducting experiments in a real environment, the hellenic public administration network SYZEFXIS. These experiments combine different traffic conditioning mechanisms through event detectors, consider IP service level agreement mechanisms that interoperate with the PBNM system and analyze the enforcement of IGP and QoS policies. Finally, validation and measurement tools are used to prove the efficiency of this framework. It is shown that this architecture offers significantly increased performance and learning capabilities, while the PBNM system achieves adaptive QoS routing through automated configuration considering the avoidance of suboptimal routing issues or under-performance conditions of the network entities.

MANETs에서 차등서비스 제공을 위한 트래픽 관리 기법 (A Traffic Management Scheme for Service Differentiation over MANETs)

  • 김관웅;배성환;김대익
    • 정보처리학회논문지C
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    • 제13C권4호
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    • pp.455-460
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    • 2006
  • 인터넷에서 차등서비스에 관한 연구는 IETF 그룹에서 표준화되었으나, Ad-hoc 네트워크와 같은 무선 환경에서 인터넷의 차등 서비스는 MAC과 같은 하위레벨 지원 없이는 구현하기가 어렵다. 현재 IEEE 802.11 프로토콜은 무선 랜 환경에 광범위하게 쓰이며 사실상의 표준으로 자리 잡았다. 그러나 차등서비스 제공을 위한 모드를 지원하지만, 성능이 낮은 것으로 나타났다. 본 논문에서는 802.11 에서 QoS 제공을 위해 우선순위를 지원하는 다중 큐 기반의 새로운 알고리즘을 제안하고 시뮬레이션을 통해 IEEE 802.11의 큐영시스템과 성능을 비교, 분석하였다. 컴퓨터 시뮬레이션 결과 제안한 알고리즘 방식의 성능이 우수했음을 보였다.

캐릭터 패션 디자인 연구(硏究) - 국내(國內) 패션업체(業體) 캐릭터 활용(活用) 현황(現況)을 중심(中心)으로 - (A Study on the Character Fashion Designs - Focusing on the domestic fashion companies using characters -)

  • 채선주;조규화
    • 패션비즈니스
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    • 제4권1호
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    • pp.1-12
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    • 2000
  • The purposes of this thesis are to investigate characteristics, environment of characters through comprehensive approach to analyze its use and strategies in apparel brands. As a method to accomplish this research, fashion related articles, documents and magazines are used along with marketing references forecasting 21st century market changes. The character industry has diverse application to different medias and also intimacy and absorbtion beyond sex, age, generation, nationality. The cultural background of character fashion is based on casual clothing caused by wide spread pursuit of sports and leisure culture, indivisualization and diversification of clothing, customer-made marketing atmosphere and tendency of pursuit of fun and humor. In case of domestic young casual market, own characters are developed for creating differentiated it's own brand images. Characters are applied as an design details or cyber fashion models standing for the image of it's own brand and take a part in other events and visual parts as well. Characters not only limited to clothing items but also further usages of characters extended to stickers and other related stationary goods are necessary. A 'Character multi shop' can be one of good methods to maximize synergy effect.

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유아의 사회도덕성에 대한 유아 연령 및 아버지 양육참여의 영향 (The Effects of Children's Age and Fathers' Childrearing Involvement on the Social Morality of Young Children)

  • 이주연;문예은
    • 한국보육지원학회지
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    • 제17권5호
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    • pp.63-82
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    • 2021
  • Objective: The purpose of this study was to examine the impact of children's age and their fathers' childrearing involvement on the social morality of young children. Methods: The data were gathered from parents who had 3-5 year old children enrolled in daycare centers and from their daycare teachers. The parents answered questions about their children's socio-demographic background and the father's childrearing involvement. And the teachers rated children's social morality. The data from a total of 245 questionnaires were analyzed by SPSS 25.0. Results: First, there was a statistically significant difference between young children's morality score and convention score. Young children's morality scores were higher than their convention scores. Second, children's age was a significant influential factor on both their moral and conventional social morality. Third, father's care-discipline had the only impact on children's conventional social morality. That is, children's social morality was higher when their fathers gave them more care and guidance. Forth, an interaction effect between children's age and father's development-support on children's conventional social morality was found. Conclusion/Implications: The results from this study suggest that a differentiated approach is needed according to the types of childrearing involvement of fathers in order to improve children's social morality.

온라인 커머스 서비스 혁신을 위한 비즈니스 생태계적 접근 (A Business Ecosystem Approach for E-commerce Service Innovation)

  • 권혁인;박주연;김주호
    • 한국IT서비스학회지
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    • 제20권4호
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    • pp.1-21
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    • 2021
  • At a time when the e-commerce market is experiencing accelerated growth, with advancements in information and communications technology (ICT), the problems of distribution of counterfeit products and consumer confusion caused by non-face-to-face purchases have increased. Hence, amid intensifying competition, it has become important for e-commerce companies deliver product information more efficiently, provide differentiated services, and secure credibility for consumers by reducing consumer damage from buying counterfeit products. However, even though consumer confusion and the inadvertent purchase of counterfeit products are intensifying in such a market scenario, there are no services that aim to solve such problems. This study examines the conventional e-commerce industry in South Korea through a political, economic, social, and technological (PEST) analysis, based on in-depth interviews with consumers, to derive the pain and gain points of the industry. As a result, the inherent problems of the e-commerce industry were revealed. Through a service value network perspective, services aimed at resolving such issues were derived, and the e-commerce business ecosystem needed to solve this problem was deduced. The findings revealed that the artificial intelligence-based service support platform has become a major driving force within the e-commerce innovation ecosystem by enabling a new way to create and secure value using ICT. This entails a new exchange mechanism and transaction architecture and a new organizational structure that breaks the barriers between industries.

Service Satisfaction and Continuous Use Intention on Omnichannel-Based Pickup Service

  • LEE, Kyoung-Hee;KIM, Bo-Young
    • 유통과학연구
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    • 제19권10호
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    • pp.5-15
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    • 2021
  • Purpose: This study aims to present individual motivation and channel characteristics affecting omnichannel service use, as well as the effect relationship of the brand factor on consumption value, service satisfaction, and continuous usability based on convenience store pickup service, one of online shopping customers' omnichannel use services. Primarily, this study divided consumption value into emotional value and functional value and examined the consumption value characteristics of omnichannel-based pickup service customers. Research design, data and methodology: A questionnaire survey was carried out targeting 324 consumers having the omnichannel-based pickup service user experience in online shopping in Korea. A confirmatory factor analysis and path analysis were carried out based on the structural equation to verify hypotheses. Results: According to the analysis result, individual motivation affected the emotional value, and the omnichannel characteristics affected functional value. The brand effect influenced both emotional and functional values. The emotional value affected continuous use intention, and the functional value affected service satisfaction. Conclusions: Therefore, consumers' emotional and functional values showed differences in consumption behavior. In online shopping companies' marketing strategy construction for omnichannel, it was confirmed that a differentiated approach is needed depending on the strategic goal of satisfaction improvement and continuous use intention consolidation.

Evaluation of Benthic Macroinvertebrate Diversity in a Stream of Abandoned Mine Land Based on Environmental DNA (eDNA) Approach

  • Bae, Mi-Jung;Ham, Seong-Nam;Lee, Young-Kyung;Kim, Eui-Jin
    • 생태와환경
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    • 제54권3호
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    • pp.221-228
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    • 2021
  • Recently, environmental DNA (eDNA)-based metabarcoding approaches have been proposed to evaluate the status of freshwater ecosystems owing to various advantages, including fast and easy sampling and minimal habitat disruption from sampling. Therefore, as a case study, we applied eDNA metabarcoding techniques to evaluate the effects of an abandoned mine land located near a headwater stream of Nakdonggang River, South Korea, by examining benthic macroinvertebrate diversity and compared the results with those obtained using the traditional Surber-net sampling method. The number of genera was higher in Surber-net sampling (29) than in the eDNA analysis (20). The genus richness tended to decrease from headwater to downstream in eDNA analysis, whereas richness tended to decrease at sites with acid-sulfated sediment areas using Surber-net sampling. Through cluster analysis and non-metric multidimensional scaling, the sampling sites were differentiated into two parts: acid-sulfated and other sites using Surber-net sampling, whereas they were grouped into the two lowest downstream and other sites using eDNA sampling. To evaluate freshwater ecosystems using eDNA analysis in practical applications, it is necessary to constantly upgrade the methodologies and compare the data with field survey methods.

애슬레저 웨어의 무역 네트워크 변화 - 사회연결망 분석 - (Changes in athleisure wear trade networks - A social network approach -)

  • 주나안;이현정;이규혜
    • 복식문화연구
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    • 제27권3호
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    • pp.251-263
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    • 2019
  • As the spread of the health and wellness trend continues worldwide, many consumers are spending much time on sports activities and expressing their individuality through sportswear. This study analyzes the trade networks of major exporters and importers of athleisure wear to provide an exporting policy for Korean apparel companies. As a result, The USA was found to import the most athleisure wear. On the other hand, China had the largest number of athleisure wear exports, and India's exports, which are becoming increasingly important as apparel producers were notable. Next, using the concept of the centrality of social network analysis, it was found that the USA was the largest importer and the center of athleisure wear's export network, but its influence has decreased gradually since 2010. China has the highest out-degree and betweenness centrality and center in the export of athleisure wear. The centrality of Asian countries such as India and Vietnam has increased. In Korea, the import of athleisure wear has increased greatly, but the export of athleisure wear has continuously decreased. Korea has less price competitiveness than other developing countries in Asia, but many Korean athleisure wear clothing brands are now attracting popularity not only in Korea but also in other countries with their excellent technology and design. In the future, the exporting policy of Korea's athleisure wear should focus on high value-added and differentiated products.