• Journal of Ginseng Research
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• v.43 no.4
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• pp.572-579
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• 2019

Background: Panax ginseng has been used in traditional medicine to strengthen the body and mental well-being of humans for thousands of years. Many elite ginseng cultivars have been developed, and ginseng cultivation has become well established during the last century. However, heat stress poses an important threat to the growth and sustainable production of ginseng. Efforts have been made to study the effects of high temperature on ginseng physiology, but knowledge of the molecular responses to heat stress is still limited. Methods: We sequenced the transcriptomes (RNA-Seq) of two ginseng cultivars, Chunpoong (CP) and Yunpoong (YP), which are sensitive and resistant to heat stress, respectively, after 1- and 3-week heat treatments. Differential gene expression and gene ontology enrichment along with profiled chlorophyll contents were performed. Results: CP is more sensitive to heat stress than YP and exhibited a lower chlorophyll content than YP. Moreover, heat stress reduced the chlorophyll content more rapidly in CP than in YP. A total of 329 heat-responsive genes were identified. Intriguingly, genes encoding chlorophyll a/b-binding proteins, WRKY transcription factors, and fatty acid desaturase were predominantly responsive during heat stress and appeared to regulate photosynthesis. In addition, a genome-wide scan of photosynthetic and sugar metabolic genes revealed reduced transcription levels for ribulose 1,5-bisphosphate carboxylase/oxygenase under heat stress, especially in CP, possibly attributable to elevated levels of soluble sugars. Conclusion: Our comprehensive genomic analysis reveals candidate loci/gene targets for breeding and functional studies related to developing high temperature-tolerant ginseng varieties.

• Research in Plant Disease
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• v.22 no.4
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• pp.264-268
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• 2016

The nursery test against rice blast in Korea from 2014 to 2015 was analyzed. The average of disease severity of leaf blast in 12 sites showed $3.7{\pm}2.1$ in 2014 and $4.4{\pm}2.1$ in 2015. Disease severity of leaf blast in Icheon and Cheolwon was increased ranging from $2.8{\pm}2.2$ in 2014 to $6.3{\pm}1.8$ in 2015. Using a designation system, a total of 588 isolates collected those years were categorized into 34 races in 2014 and 51 races in 2015 based on the reaction pattern of Korean differential varieties. The blast isolates of 2015 were more diverse than those in 2014. The ratios of KI race to KJ race were 54:46 in 2014 and 70:30 in 2015; however, the predominant race population was KJ-301 as 16%, and KI-101 as 15% in 2014 and 2015, respectively. These results indicate that the distribution of the blast races is getting more diverse in Korea, therefore, this research would provide the possibility to predict race distribution and change to prevent the outbreak of rice blast and will also serve as a useful information for breeding of resistant rice cultivar against blast.

• Food Science and Preservation
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• v.19 no.4
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• pp.532-538
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• 2012

Seven rice varieties (Dasan, Keunseon, Goami, Baekjinju, Seolgaeng, Hangangchal and Heukseol) were used to study the physicochemical properties of dry milled (200-mesh) rice flour species. The moisture and crude protein contents of rice were 15.00-15.10% and 6.09-8.21%, respectively. The crude lipid and crude ash of rice were 0.21-1.02% and 0.37-1.62%, respectively. As for the Hunter's color value, the L value was highest in the Dasan flour (96.47); the a value was highest in the Heukseol flour (5.03); and the b value was highest in the Baekjinju flour (3.36). The water aborption index was highest in the Goami flour (1.45), and the water solubility index was highest in the Hangangchal flour (9.16%). The amylose contents of the rice flour species were highest in the Goami (26.42%) rice flour, followed by the Dasan (19.39%), Seolgaeng (19.24%), Keunseon (18.06%), Heukseol (15.52%), Baekjinju (9.16%), and Hangangchal (0.84%) rice flour. In the X-raydiffractin patterns of the diverse species, seven tice varieties showed A-type crystallinity. As for the amylogram properties, the initial pasting temperature was 58.00-$69.03^{\circ}C$. The maximum viscosity was highest in the Dasan flour. The Heukseol flour had the lowest maximum viscosity, breakdown, and setback. In terms of the thermal properties of the differential scanning calorimeter (DSC), the onset temperature was 59.03-$66.84^{\circ}C$; the peak temperature, 66-70-$72.82^{\circ}C$; and the end temperature, 74.06-$78.66^{\circ}C$. The enthalpy (${\Delta}H$) was lowest in the Heukseol flour (7.59 J/g) and highest in the Seolgaeng flour (11.36J/g).

• Korean Journal of Food Science and Technology
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• v.42 no.5
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• pp.613-619
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• 2010

Three varieties of taro flours (Altoran, Josaengjong, and Jaeraejong) were analyzed to evaluate their nutrient composition and physicochemical properties. Moisture contents of the three taro cultivars varied from 5.74-10.30%. Among the three cultivars, Altoran flour had higher protein and fat contents than other cultivars. There were 17 kinds of amino acids in the three taro flours. The major fatty acids in the three taro flours were linoleic acid (46.5-51.4%), palmitic acid (21.7-25.8%), and oleic acid (12.3-18.7%), and two thirds of the total fatty acids were unsaturated fatty acids. Potassium, phosphorus, magnesium, and calcium were the most plentiful minerals in the three taro flours, and the free sugars were glucose, fructose, and sucrose. The total dietary fiber contents of the three taro flours ranged from 12.97 to 17.60%. From these results, Altoran contained a high level of dietary fiber, minerals, essential amino acids, and free sugars. Jaeraejong showed the highest water absorption index. The water soluble index of Altoran was higher than that of the other cultivars. From the differential scanning calorimeter results, the gelatinization temperatures of the three taro flours were $84.98-89.91^{\circ}C$. The pasting temperature of the three taro flours was from 46.77 to $49.30^{\circ}C$ by rapid visco-analyzer. Among these varieties, Josaengjong had the highest peak viscosity and final viscosity, whereas Altoran had the lowest. These taro cultivars are expected to be potential material for application in taro processed foods, due to their gelatination and pasting properties.

• Korean journal of applied entomology
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• v.23 no.4 s.61
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• pp.203-208
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• 1984

Four varieties of tobacco, Nicotiana tabacum var. NC 2326. NC 95, NC 744 and Havana. and two plant species, Physalis floridana Rydb., N. repanda Wild. were used for classification of ten isolates of potato virus Y(PVY) obtained Iron potato or tabacco plants showing various symptoms. Each of the 10 isolates could be distinguished by the host reactions showing necrosis, vein banding and/or mottling, or no symptom on these hosts. Five isolates of PVY, PVY-VN, PVY-N, PVY-NSNR, PVY-Chile, and PVY-Argentina, showed necrotic symptom on NC 2326, but others showed vein handing and/or mottling symptom. On NC95, each of the isolates tested showed similar symptons as on NC2326, except necrotic symptom by the isolate PVY-MSNR. Havana showed mottling reaction to the PVY-NSNR, PVY-MSNR, PVY-MSMR, PVY-VB, and necrosis to PVY-Chile, PVY-Argentina, but showed no symptom to the others. NC744 showed mottling symptom to the PVY-MSNR and PVY-MSMR, necrosis to the PVY-Chile and PVY-Argentina, and no symptom to the others. On N. repanda, necrotic reaction to PVY-Argentina. no symptom to PVY-VN and PVY-C, and nettling to others were observed.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.286-293
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• 2012

The resistance to Tobamovirus in Capsicum spp. has been known to be controlled by five different alleles ($L^0$, $L^1$, $L^2$, $L^3$, and $L^4$) of L locus on the telomere of long arm of pepper chromosome 11. To develop a set of molecular markers differentiating all the alleles of L locus, we used five pepper differential hosts including Capsicum annuum Early California Wonder (ECW, $L^0L^0$), C. annuum Tisana ($L^1L^1$), C. annuum Criollo de Morelos 334 (CM334, $L^2L^2$), Capsicum chinense PI 159236 ($L^3L^3$), and Capsicum chacoense PI 260429 ($L^4L^4$). Developing a series of CAPS or SCAR markers specifically linked to the alleles was allowed by the sequence comparison of PCR amplicons of the $L^3$-linked markers (189D23M, A339, and 253A1R) and BAC sequences (FJ597539 and FJ597541) in the pepper differentials. Genotypes deduced by these markers in 48 out of 53 $F_1$ hybrids of commercial pepper varieties were consistent with their phenotypes by bioassay using Tobamovirus pathotypes ($P_0$, $P_1$, and $P_{1,2$). Consequently, these markers can be useful to differentiate L alleles and for breeding Tobamovirus resistance in pepper with marker-assisted selection.

• Korean journal of applied entomology
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• v.7
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• pp.69-75
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• 1969

These studies aimed to investigating the pathogenicity of Fusarium isolates from the wilted cucur bitaceous plants and the pathogenicity of 24 isolates to cucumber, oriental cantaloup, oriental pickling melon, sponge gourd. muskmelon and watermelon were as follows. 1) The isolates from wilted watermelon and melon severely and one of the isolates was slightly pathogenicon oriental pickling melon or oriental cantaloup. 2) The isolates from wilted cucumber were four types. some of the isolates were severely pathogenic on cucumber and infected oriental cantaloup. oriental pickling melon and melon. Some of the isolates were not pathogenic or slightly pathogenic on cucumber. but these were severely pathogenic on oriental pickling melon. One of the isolates was severely pathogenic on oriental cantaloup oriental pickling melon and melon, but no pathogenic on cucumber and one of the isolates was also severely pathogenic on oriental cantaloup. oriental pickling melon and melon and moderatly pathogenic' on water melon. 3) The isolates from wilted oriental cantaloup were four types some of the isolates infected oriental cantaloup, oriental pickling melon and melon severely and some of the isolates were pathogenic on oriental cantaloup. oriental pickling melon. melon and watermelon. One of the isolates, was slightly pathogenic on oriental pickling melon and melon but one of the isolates was highly pathogenic on melon and infected watermelon slightly. 4) Wilt Fusarium of the cucurbitaceous plants could divide into the group of wilt Fusarium of watermelon, cucumber and muskmelon according to the pathogenicity but it will have to rearrange to one form species from several form species of wilt Fusarium of the cucurbitaceous plants and ought to divide to races according to pathogenicity on severely fixed differential varieties which selected from the cucurbitaceous plants. because of the degree of pathognicity and host range are not surely fixed between isolates of wilt Fusarium of the cucurbitaceous plants tested.

• Korean journal of applied entomology
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• v.13 no.4 s.21
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• pp.227-231
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• 1974

During the period from 1971 to 73 physiologic races of potato late blight pathogen, Phytophthora infestans, causing severe losses of seed potato production at Alpine area, Korea, were investigated. In 1971, 68 isolates of the pathogen from various potato varieties were identified by the use of differential host Plants, and found that there were 10 races; 0, 1, 2, 3, 1.3, 1.4, 3.4, 1.3.4, 2.3.4. The most Predominant races for the year were 0 and 3. In 1972, the same procedure was repeated with 62 isolates of the pathogen, and found 5 different races; 0, 1, 4, 1.3 and 1.3.4. The most predominant race for the year was race 0. In 1973, six races; 0, 3, 4, 1.3, 3.4 and 1.3.4 were found from 76 isolates of the year, and the race 0 and 4 apperared as the most Predominant races for the year. Total of 11 races; 0, 1, 3, 4, 1.3, 1.4, 3.4, 1.3.4, 2.3.4 and 1.2.3.4, were found during the period from 1971 to 73 at Alpine area, among which the most predominant races were race 0, 3, and 4. The investigation indicated that the anual incidence of these races largely depended on the climatic conditions of the year.

• Research in Plant Disease
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• v.18 no.2
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• pp.86-92
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• 2012

Clubroot caused by Plasmodiophora brassicae Woron. is one of the most important diseases in Brassica crops worldwide. To establish more simple and reliable screening method for resistant cabbage and broccoli to P. brassicae, the development of clubroot on the plants according to inoculum concentration and incubation period after inoculating with the pathogen was investigated using P. brassicae GN1 isolate (race 9). To facilitate and acquire precise result of resistance screening of cabbage and broccoli to clubroot, 14-day-old seedlings were inoculated by drenching roots with the spore suspension of P. brassicae to give inoculum density of $2.5{\times}10^9$ spores/pot. To develop the disease, the inoculated seedlings were incubated in a growth chamber at $20^{\circ}C$ for 3 days, and then cultivated in a greenhouse ($20{\pm}5^{\circ}C$) for five weeks. Under the optimum conditions, 16 cabbage and 17 broccoli cultivars were tested for resistance to four field isolates (GN1, GN2, GS and YC) of P. brassicae collected from four regions in Korea. Among them, some cabbage and broccoli cultivars showed different resistance response to three isolates (GN1, GN2 and GS) determined as race 9 by using the differential varieties of Williams. On the other hand, all the tested cultivars were highly susceptible to YC isolate (race 2). The results suggest that this method is efficient screening method of cabbage and broccoli for resistance to P. brassicae.

• Korean Journal of Food Science and Technology
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• v.30 no.4
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• pp.787-793
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• 1998

Physicochemical properties of starch of three cultivars of Korean kidney Bean Starches, Pink (PKB), Red (RKB) and White (WKB) were studied. Starch granule was oval/round and smooth in all samples. The amylograms showed a continuous increase of viscosity without peak during heating. The water-binding capacities of starches of PKB, RKB and WKB were 102.1%, 94.7% and 106.9%, respectively. The swelling powers were rapidly incresed in all samples. The amylose content, blue value and relative viscosity of kidney bean starches were $31.1{\sim}32.8%,{\;}0.64{\sim}0.66$ and $2.27{\sim}2.61{\;}mlg^{-1}$, respectively. The transmittance of starch suspension was linearly increased as the temperature raised from $65^{\circ}C{\;}to{\;}85^{\circ}C$. The gelatinization temperature ranges determined by differential scanning calorimetry (DSC) were $71.1{\sim}86.9^{\circ}C for PKB, $71.1{\sim}86.0^{\circ}C$ for RKB and $60.8{\sim}77.9^{\circ}C$ for WKB.