• Molecular & Cellular Toxicology
• /
• v.4 no.4
• /
• pp.293-299
• /
• 2008

To screen the differentially expressed genes in cadmuim-exposed marine medaka fish (Oryzias javanicus), a candidate marine test fish for ecological toxicity, the differential display polymerase chain reaction (DD-PCR) was carried out, since the genome-wide gene expression data are not available in this fish species yet. A total of 35 clones were isolated from cadmium-exposed fish and their nucleotide sequences were analyzed. The differentially expressed gene candidates were categorized to response to stimulus (3); ion binding (3); DNA binding (1); protein binding (6); carbohydrate binding (1); metabolic process (4); biological regulation (3); cellular process (2); protein synthesis (2); catalytic activity (2); sense of sight (1); immune (1); neurohormone (1); signaling activity (1); electron carrier activity (1) and others (3). For real-time quantitative RT-PCR, we selected catalase, glucose-6-phosphate dehydrogenase, heat shock protein 70, and metallothionein and confirmed that cadmium exposure enhanced induction of these four genes.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2009.10a
• /
• pp.1081-1085
• /
• 2009

We have already developed EMI reducing techniques using lossless compression by vertically differential EMI suppression method (VDE[1]). It applies lossless modulo reduction and data bit mapping optimization for low voltage differential signaling (LVDS) transmission lines, that reduces the probability of transient bit and EMI by 12 dB [6][7]. We also improved and optimized the VDE for low power LCD interface. With this modified VDE algorithm[8], the developed FPGA was measured the reduction of the power consumption of LCD circuit by more than 15 % compared to the conventional methods in the case of 14-in LCD with SXGA resolution. The VDE algorithm is based on the total image systematic approach. In the VDE method, the present image signals are subtracted for the 1H delayed image signals and transferred to a column driver through a PCB. As the vertical correlations for image signals are very high, we expected that most of the vertically subtracted image signals remain 0 level and transient cycles become very long. As a result, the power consumption and EMI are extremely reduced for the transferred image signals on a PCB. In this paper, we discussed our proposed method by emphasizing the fact that systematic approach are important based on not only display point of view but also total system point of view.

• Biomedical Science Letters
• /
• v.19 no.2
• /
• pp.118-123
• /
• 2013

Tumor necrosis factor-alpha (TNF-${\alpha}$) is a proinflammatory cytokine that mediates the inflammatory response and immune functions, and modulates the proliferation, differentiation and cell death of cancer cells. The differential functions of TNF-${\alpha}$ in various human cells due to the formation of different stimulating pathway upon the binding of TNF-${\alpha}$ to its receptors. In the present study, we examined the different effects of TNF-${\alpha}$ on the survival and apoptosis between normal granulocytes and human myeloid leukemia HL-60 cells. Although TNF-${\alpha}$ did not affect on the constitutive apoptosis of granulocytes, TNF-${\alpha}$ strongly induced the apoptosis of HL-60 cells in a dose- and a time-dependent manner. TNF-${\alpha}$-induced apoptosis was occurred via the activation of caspase 8, caspase 9 and caspase 3/7 and the induction of ROS production in HL-60 cells. Also, BAY-11-7085, a NF-${\kappa}B$ inhibitor, blocked the TNF-${\alpha}$-induced apoptosis in HL-60 cells. NF-${\kappa}B$ may be involved in TNF-${\alpha}$-induced apoptotic signaling pathway in HL-60 cells. These results suggest that TNF-${\alpha}$ activates apoptotic pathways and its process depends on cell type and many cellular factors. A better understanding of the differential effect of TNF-${\alpha}$ on cell apoptosis and survival may provide important information that can be used to elucidate the specific inhibitory effect of TNF-${\alpha}$ on the cancer dis.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.14 no.6
• /
• pp.1061-1068
• /
• 2019

Cooperative networks transmit signals form the source node to the destination node via several relay node where the combining and demodulation of relay aided signals provide the benefit of performance enhancement and data rate increment. In general, a repetitive manner transmission scheme in which the received signal from the source node is amplified/re-generated and forward to the destination node is widely used. In this paper, we analyzed the performance of cooperative networks using the mixed transmission scheme. The conventional modulation scheme is used in the source-relay links, and space-time code is applied in the relay-destination links. To reduce the complexity of the overall system, we adopt differential modulation which bypasses channel state information. We analyze bit error rate (BER) of the proposed system by considering the number of relay nodes, and the performances depending on the strength of transmission signal in the source-relays and rely-destination links are compared. In addition, we also discuss the system performance with the signal strength and the number of relay nodes simultaneously.

• Animal cells and systems
• /
• v.14 no.2
• /
• pp.83-89
• /
• 2010

Gangliosides are a ubiquitous component of the membranes of mammalian cells that have been suggested to play important roles in various cell functions such as cell-cell interaction, adhesion, cell differentiation, growth control and signaling. However, the role that gangliosides play in the immune rejection response in xenotransplantation is not yet clearly understood. In this study, differential expression patterns of gangliosides in HEK293 (human embryonic kidney cells), PK15 (porcine kidney cells), NIH-kd (NIH-mini pig kidney cells, primary cultured) and the cortex, medulla and calyx of the NIH-mini pig kidney were investigated by high-performance thin-layer chromatography (HPTLC). The results revealed that HEK293, PK15 and NIH-kd contained GM3, GM2 and GD3 as major gangliosides. Moreover, GM3, which are the gangliosides of NIH-kd, were expressed at higher levels than HEK293 and PK15. Especially, GT1b were expressed in HEK293 and NIH-kd but not in PK15. Finally, GM1 and GD1a were expressed in NIH-kd, but not in HEK293 or PK15. These results suggest that differential expression patterns of gangliosides from HEK293, PK15 and NIH-kd are related to the immune rejection response in xenotransplantation.

• Biomolecules & Therapeutics
• /
• v.7 no.4
• /
• pp.307-314
• /
• 1999

The human muscarinic acetylcholine receptor (mAChR) subtypes Hml, Hm2 and Hm3 have been expressed in insect cells (Spodoptera frugiperda, Sf9) using the baculovirus expression system. Expression of relevant DNA, transcript and receptor proteins was identified by PCR, Northern blotting and [$^{3}H$]QNB binding, respectively. As assessed by [$^{3}H$]QNB binding sites, yields of muscarinic receptors in membrane preparations in this study were as about 5-20 times high as those in mammalian cells reported in previous studies. The [$^{3}H$]QNB competition binding studies with well-known subtype-selective mAChR antagonists showed that the receptors expressed in Sf9 cells retain the pharmacological characteristics expected for the ml , m2 and m3 muscarinic receptors. The ml-selective antagonist, pirenzepine, displayed a considerably higher affinity for Hml by 110-fold and 35-fold than for Hm2 and Hm3, respectively, The m2-selective methoctramine displayed a significantly higher affinity for Hm2 than for Hml and Hm3 (10- and 26-fold, respectively). p-F-HHSiD exhibited high affinity for Hm3 that is not significantly different from those for Hml, but 66-fold higher than its affinity for Hm2. The functional coupling of the recombinant receptors to second messenger systems was also examined. While both Hml and Hm3 stimulated phosphoinositide hydrolysis upon activation by carba-chol, Hm2 produced no response. On the other hand, activation of mAChRs induced the inhibition of forsko-lin-stimulated cyclic AMP formation in Hm2-expressing cells, whereas the significant dose-dependent increase in or poor response on cyclic AMP formation were produced in Hml or Hm3-expressing cells, respectively. These results indicate the differential coupling of recombinant Hml, Hm2 and Hm3 receptors expressed in SF9 cells to intracellular signalling system.

• Journal of Biomedical and Translational Research
• /
• v.19 no.4
• /
• pp.116-124
• /
• 2018

Korean Native Pig (KNP) has a uniform black coat color, excellent meat quality, white colored fat, solid fat structure and good marbling. However, its growth performance is low, while the western origin Yorkshire pig has high growth performance. To take advantage of the unique performance of the two pig breeds, we raised crossbreeds (KNP ${\times}$ Yorkshire to make use of the heterotic effect. We then analyzed the liver transcriptome as it plays an important role in fat metabolism. We sampled at two stages: 10 weeks and at 26 weeks. The stages were chosen to correspond to the change in feeding system. A total of 16 pigs (8 from each stage) were sampled and RNA sequencing was performed. The reads were mapped to the reference genome and differential expression analysis was performed with edgeR package. A total of 324 genes were found to be significantly differentially expressed (${\left|log2FC\right|}$ > 1 & q < 0.01), out of which 180 genes were up-regulated and 144 genes were down-regulated. Principal Component Analysis (PCA) showed that the samples clustered according to stages. Functional annotation of significant DEGs (differentially expressed genes) showed that GO terms such as DNA replication, cell division, protein phosphorylation, regulation of signal transduction by p53 class mediator, ribosome, focal adhesion, DNA helicase activity, protein kinase activity etc. were enriched. KEGG pathway analysis showed that the DEGs functioned in cell cycle, Ras signaling pathway, p53 signaling pathway, MAPK signaling pathway etc. Twenty-nine transcripts were also part of the DEGs, these were predominantly Cys2His2-like fold group (C2H2) family of zinc fingers. A protein-protein interaction (PPI) network analysis showed that there were three highly interconnected clusters, suggesting an enrichment of genes with similar biological function. This study presents the first report of liver tissue specific gene regulation in a cross-bred Korean pig.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.12 no.1
• /
• pp.10-17
• /
• 2012

This paper presents a clock regenerator using two $2^{nd}$ order ${\sum}-{\Delta}$ (sigma-delta) modulators for wide range of dividing ratio as defined in HDMI standard. The proposed circuit adopts a fractional-N frequency synthesis architecture for PLL-based clock regeneration. By converting the integer and decimal part of the N and CTS values in HDMI format and processing separately at two different ${\sum}-{\Delta}$ modulators, the proposed circuit covers a very wide range of the dividing ratio as HDMI standard. The circuit is fabricated using 0.18 ${\mu}m$ CMOS and shows 13 mW power consumption with an on-chip loop filter implementation.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2002.11a
• /
• pp.74.1-74
• /
• 2002

• Proceedings of the IEEK Conference
• /
• 2006.06a
• /
• pp.621-622
• /
• 2006

This paper presents the design of LVDS(Low-Voltage-Differential-Signaling) driver circuit for Gb/s-per-pin operation using BiCMOS process technology. To reduce chip area, LVDS driver's switching devices were replaced with lateral bipolar devices. The designed lateral bipolar transister's common emitter current gain($\beta$) is 20 and device's emitter size is 2*10um. Also the proposed LVDS driver is operated at 2.5V and the maximum data rate is 2.8Gb/s approximately.