• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.123-123
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• 2017

In spite of the potential medicinal significance and a wide range of pharmacologic properties of Platycodon grandiflorum, the molecular mechanism of its roots is still unknown. The present study was conducted to profile proteins from 3, 4 and 5 months aged diploid and tetraploid roots of Platycodon grandiflorum using high throughput proteome approach. Two-dimensional gels stained with CBB, a total of 68 differential expressed proteins were identified from the diploid root out of 767 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 29 differential expressed protein spots (${\geq}2-fold$) were analyzed using LTQ-FTICR MS whereas a total of 24 protein spots were up-regulated and 5 protein spots were down-regulated. On the contrary, in the case of tetraploid root, a total of 86 differential expressed proteins were identified from tetraploid root out of 1033 protein spots of which a total of 39 differential expressed protein spots (${\geq}2-fold$) were analyzed using LTQ-FTICR MS whereas a total of 21 protein spots were up-regulated and a total of 18 protein spots were down-regulated. It was revealed that the identified proteins from the explants were mainly associated with the nucleotide binding, oxidoreductase activity, transferase activity. Taken together, the identified proteins may be helpful to identify key candidate proteins for genetic improvement of plants.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.286-286
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• 1996

In order to elucidate the mechanism of action of transdermal absorption-enhancing compounds, i.e., pyrrolidone derivatives (2-pyrrolidone, 1-methyl-2-pyrrolidone, 1-ethyl-2-pyrrolidone, 1,5-dimethyl-pyrrolidone and 5-methyl-2-pyrrolidone), multilamellar liposome was prepared from the simulated stratum corneum lipid and employed as a model system for the barrier function of the stratum corneum. The liposomal membrane of the stratum corneum lipid liposome (SCLL) behaves as an osmometer and has an excellent barrier function. In addition, its phase transition temperatures are similar to those of human stratum corneum intercellular lipid region. Therefore, SCLL seems to be a useful skin model. To estimate the barrier function of SCLL, the osmotic behavior of SCLL was measured in the presence of pyrrolidone derivatives and the effect on the phase transition temperature of SCLL was also investigated using differential calorimetry. Above a certain concentration (MLAC), enhances perturb the barrier function of the liposome. The relationship between MLACs and the partition coefficient of the pyrrolidone derivatives was observed; the greater the partition coefficients, the smaller the MLAC. This suggests that the more hydrophobic enhancers penetrate into the lipid layer more easily and reduce the barrier function of membrane more effectively. The results of differential scanning thermograms of the SCLL suggest that the pyrrolidone derivatives had incorporated into the lipid layer in the liposome and increased the fluidity of the lipid layer in the liposome. Such activity might have some correlation with the transdermal absorption-enhancing activity these compounds.

• The Plant Pathology Journal
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• v.19 no.1
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• pp.34-42
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• 2003

The ascomycete Magnaporthe grisea is a pathogen of rice blast and is known to form specialized infection structures called appressoria for successful infection into host cells. To understand the molecular mechanism underlying infection process, appressorium-related genes were identified through global approaches including EST sequencing, differential hybridization, and sup-pression subtractive hybridization. EST database was generated on >2,000 cDNA clones randomly selected from appressorium stage cDNA library. Large number of ESTs showed homology to known proteins possibly involved in infection-related cellular development (attachment, germination, appressorium formation, and colonization) of rice blast fungus. The 1051 ESTs showing significant homology to known genes were assigned to 11 functional categories. Differential hybridization and suppression subtractive hybridization were applied to identify genes showing an appressorium stage specific expression pattern. A number of genes were selected as up-regulated during appressorium formation compared with the vegetative growing stage. Clones from various cDNA libraries constructed in different developmental stages were arrayed on slide glass for further expression profiling study. functional characterization of genes identified from these global approaches may lead to a better understand-ing of the infection process of this devastating plant disease, and the development of novel ways to protect host plant.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.11
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• pp.5574-5591
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• 2017

Inspired by the idea of Artificial Immune System, many researches of wireless sensor network (WSN) intrusion detection is based on the artificial intelligent system (AIS). However, a large number of generated detectors, black hole, overlap problem of NSA have impeded further used in WSN. In order to improve the anomaly detection performance for WSN, detector generation mechanism need to be improved. Therefore, in this paper, a Differential Evolution Constraint Multi-objective Optimization Problem based Negative Selection Algorithm (DE-CMOP based NSA) is proposed to optimize the distribution and effectiveness of the detector. By combining the constraint handling and multi-objective optimization technique, the algorithm is able to generate the detector set with maximized coverage of non-self space and minimized overlap among detectors. By employing differential evolution, the algorithm can reduce the black hole effectively. The experiment results show that our proposed scheme provides improved NSA algorithm in-terms, the detectors generated by the DE-CMOP based NSA more uniform with less overlap and minimum black hole, thus effectively improves the intrusion detection performance. At the same time, the new algorithm reduces the number of detectors which reduces the complexity of detection phase. Thus, this makes it suitable for intrusion detection in WSN.

• The KIPS Transactions:PartC
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• v.11C no.1
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• pp.115-122
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• 2004

Diff-Serv is a mechanism by which network service providers can offer differing levels of network service to different traffic, in so providing quality of service (QoS) to their customers. Because this mechanism has been deployed just for fixed hosts with the Token Bucket mechanism, DiffServ have been suggested can not satisfy the mobility service or the differential serrlce for Individual traffics. In this paper, we suggest WFQ mechanism for traffic conditioner and scheduling method for monitoring the AggF(Aggregate Flow) which will be controlled in edge nodes and border routers. So it will control traffic rate dynamically and suggest efficient usability of bandwidth.

• Restorative Dentistry and Endodontics
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• v.23 no.2
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• pp.676-681
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• 1998

The purpose of this study is to explain theoretically the mechanism of the frequency-dependent apex locator which can detect the apex of the root in moist canals with blood or other conductible canal irrigants. The frequency-dependent apex locator is based on measuring the ratio of the two impedances of the two alternating currents with different frequencies. We analyzed the changes of the ratio between the two impedances by using differential calculus. Our analysis shows that : 1. When the file is in the moist canal, the ratio between the two impedances is almost constant. 2. As the file approaches the apex of the root, the ratio decreases sharply. By this mechanism, the frequency-dependent apex locator can detect the apex of the-root quite accurately in moist canals.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.41 no.2
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• pp.31-40
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• 2004

Diff-Serv(DS) is a mechanism by which network service providers can offer differing levels of network service to different traffic, in so providing quality of service(QoS) to their customers. Because this mechanism has been deployed just for fixed hosts with the Token Bucket mechanism according to AggF(Aggregate Flow) instead of each flow, DS can not suggest effective usability of traffic resources. In this paper, we use WFQ mechanism for traffic conditioner and scheduling method monitoring the AggF and the results will be used to control the next flows coming in TC. So it will control traffic rate dynamically and suggest efficient usability of bandwidth.

• Earthquakes and Structures
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• v.24 no.4
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• pp.247-258
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• 2023

Multiple pounding tuned rolling mass damper (MPTRMD) distributed in the cavity of voided slabs is proposed to passively control multi-story frame structures, which disperses the mass of the oscillator to multiple dampers so that the control device can be miniaturized without affecting the vibration control performance. The mechanism and the differential motion equations of the MPTRMD-controlled multi-degree-of-freedom system are derived based on the Lagrange principle. Afterward, this advanced RMD is applied to a simplified 20-floor steel frame to evaluate the seismic control performance in the numerical analysis. A four-storey frame structure equipped with MPTRMD is then taken for a shaking table test to verify its effectiveness of control performance. The pounding mechanism has been detailed studied numerically and experimentally as well. The numerical and experimental results show that the proposed damper is practically promising not only for its prominent control performance but also for its lightweight and space-saving. Additionally, the pounding mechanism influenced by the variable impact parameters exhibits a balance between the two effects of motional limitations and energy dissipation.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2002.05a
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• pp.162-168
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• 2002

This paper shows the failure(wear) phenomena of differential bearings in the transaxle of passenger cars and investigate their characteristics. It was found that the wear mechanism was mild abrasive wear caused by the presence of particles in the gear box. The sides of the outer raceway was more neared than center of it, so it is showed as if the crowning of outer raceway are increased. With close examination of the failed bearing, various countermeasures could be suggested.

• International Journal of Precision Engineering and Manufacturing
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• v.3 no.3
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• pp.28-36
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• 2002

The continuously variable transmission (CVT) mechanism considered here is the output coupled type which combines the functions of a 2K-H I type differential gear unit and a V-belt type continuously variable unit (CVU). One shaft of the CVU is connected directly to the output shaft and another shaft of it is linked to the differential gear unit. It is shown that some fundamental relations (speed ratios, power flows and efficiencies) for twelve mechanisms previously described are valid by various experimental studies, six of them produce a power circulation and the others produce a power split. Parametric analysis is carried out in relation to the efficiency, speed ratio and power ratios in order to assist in the design of an optimum configuration. Some useful properties associated with power flow modes also are discussed in the output coupled type continuously variable transmission.