• Journal of Plant Biotechnology
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• 제7권2호
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• pp.123-127
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• 2005

The evaluation of source potential and sink strength is the generally large and laborious sample size required to adequately assess anyone of the parameters in field-grown sweetpotato. For this purpose we used the rooted single-leaf cuttings with petioles, because the source and sink organs are restricted in this system. The rooted single-leaf cutting of sweetpotato provides a unique source-sink model system, and is established within about 50 days after planting. In this study, the sink potential of sweetpotato tubers was examined based on the expression of genes for starch synthesis (AGPase) and modification (SBEII and GBSSI) in single rooted leaf plant. The gene expression patterns of GBSSI, SBEII and AGPase at various developmental stages and in different types of root tissues presented. These results suggest that the rooted single-rooted method can be used an ideal model system to study physiological and biochemical mechanisms in sweetpotato.

• Journal of Plant Biotechnology
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• 제1권2호
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• pp.91-95
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• 1999

Genetic engineering of stone fruit species like apricot, plum, peach and cherry are hampered by the inefficient and low-level regeneration processes in tissue culture. The first transgenic stone fruit species have emerged from transformed hypocotyls. These great achievements were applauded by the scientific community contrary the fact that hypocotyl derived transgenic plants have no real brooding value. Tissue culture of different organs of valuable cultivars are recorded with an extremely low-level of regeneration in the literature. To improve the tissue culture basis of stone fruit plants an extensive tissue culture programme were launched and dozens of different media were compared including a series of hormone concentration in the tissue culture systems. Our continuous efforts were crowned by a very efficient method for achieving up to 30-40% regenerable petioles. Usually on a single petiole several well-separated meristems were induced. After 3-4 weeks of cultivation shoots were developed. The basic media $K_2$ were supplemented with 10g/L saccharose, 10g/L glucose and 10g/L maltose. The following plant hormones were used BAP 1mg/L, TDZ 1mg/L, 2-iP 1mg/L and IAA 0,1 mg/L concentrations. The Petri dishes were kept for 3 weeks in dark at a temperature 22$^{\circ}C$ for 8 hours and 22-24$^{\circ}C$ for 16 hours. The Petri dishes were sealed with Parafilm. The regeneration of the petioles were genotype independent and we were able to regenerate different plum cultivars with almost the same efficiency.

• 한국한의학연구원논문집
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• 제8권1호
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• pp.55-63
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• 2002

The plant origines of Atractylodis Rhizoma Alba and Atractylodis Rhizoma are genus Atractylodes*Compositae). Their origines in pharmacopoeia are slightly different among Korea, Japan, North Korea and China. The species of Atractylodis Rhizoma Alba are Atrctylodes japonica $K_{OIDZ}$. A. ovata $T_{HUNB}$.. Atractylodis Rhizoma are A. lancea DC., A. chinensis DC. and A. koreana $K_{ITM}$. But the texonomic genealogy of Atractylodis Rhizoma Alba is different from that of Atractylodis Rhizoma. The herbal origines of Atractylodis Rhizoma Alba and Atractylodis Rhizoma were not different at the early days. Since Tao-Hong-Jing(陶弘景) distinguished between Atractylodis Rhizoma Alba and Atractylodis Rhizoma at the very beginning, theri medicinal usages are different, Atractylodis Rhizoma have been used to remove pathogenic dampness in the digestive organs and Atractylodis Rhizoma Alba have been used to invigorate the digestive system and replenish qi. In additions, the figures and historical records of herbal appearance are different between Atractylodis Rhizoma Alba and Atractylodis Rhizoma. The diagnostics of them are in accord with the texonomic genealogies.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.25-25
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• 2017

Inflorescence architecture mainly contributes to final grain yield in crops. Sorghum inflorescence is basically composed of one fertile sessile spikelet (SS) and two infertile pedicellate spikelets (PS). To identify regulatory factors involved in the inflorescence architecture, we screened an EMS mutagenesis population from the pedigreed sorghum mutant library. We found inflorescent architecture mutants, named as multi-seed mutants, msd, with gained fertile ability in PS and also an increased number of floral branches. In natural sorghum populations, it is not common that are fertile. A detailed dissection of developmental stages of wild type and msd1 mutant described that the PS in wild type do not have floral organs, including ovary, stigma, filament and anther, while the msd1 mutants generate intact floral organ in the sessile spikelet. We found MSD1 encoded a TCP transcription factor using bulk segregant analysis (BSA) of F2 population, and was a strongly enriched expression during inflorescence developmental stages. We proposed that MSD1 functions to suppress floral organ maintenance at PS during inflorescence development in Sorghum. To explore the regulatory network associated with PS fertility, whole genome expression profiling was performed at 4 different developmental stages in 6 various tissue types between wild type and msd1. Taken together, we demonstrated that MSD1 was involved in the plant hormone and maybe influenced program cell death in PS via the activation of plant hormonal pathway.

• 식물조직배양학회지
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• 제24권6호
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• pp.329-334
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• 1997

미류나무의 잎절편들을 fructose를 첨가한 WPM에서 배양하였으나, TDZ와 BA 그리고 NAA의 여러 농도와의 조합에서 다른 형태의 기관들(뿌리나 줄기)이 관찰되었으며, 체세포 배와 유사한 기관들의 형태도 관찰되었다. 3계통 미류나무들의 잎절편들을 사이토카이닌(BA 또는 TDZ)와 오옥신(NAA)를 첨가한 WPM 배지에서 접종시켜, 재분화를 유도한 결과, BA (05 mg/L)나 TDZ (0.01 mg/L)와 NAA (0.05 mg/L 또는 0.1 mg/L)의 조합에서 재분화 율은 각각 63%에서 100%이고, 재분화 개체 수는 잎절편당 평균 2.5부터 20개, 그리고 부정줄기 형성체군(organogenic site) 들은 평균 1.8에서 4.5개로 높게 나타났다. 또한 세 계통의 미류나무들은 각기 유사한 처리 조합에서 높은 재분화율을 나타내었다.

• Journal of Plant Biotechnology
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• 제39권3호
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• pp.146-153
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• 2012

In this study, the antioxidant property of leaf and callus extracts of five selected in vitro grown Ocimum species (Ocimum sanctum, Ocimum kilimandscharicum, Ocimum gratissimum, Ocimum basilicum, and Ocimum americanum) and their respective callus extracts was investigated. The callus cultures were successfully initiated on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) (1mg L) combined with different concentrations (0.1-0.4 mg L) of kinetin as plant growth regulators. Total phenolic contents were estimated using the Folin-Ciocalteu reagent. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power, $Fe^{2+}$ chelating activity, and ${\beta}$-carotenelinoleic acid bleaching assays were used to determine the biological effects of the extracts. Interestingly, all the callus extracts exhibited significant (p<0.05) increase in phenolic contents and antioxidant activity. Furthermore, a liner correlation was obtained between the total phenolic contents and free radical scavenging activity ($R^2$ = 0.783). The extracts of leaves and calluses of Ocimum species exhibited activity in all the in vitro antioxidant assays, but its extent was less potent that the positive controls butylated hydroxyl anisole (BHA) and ascorbic acid. A higher accumulation of phenolics in the callus extracts suggests that isolation of high-concentration materials with antioxidant activivity is possible from in vitro callus cultures rather than field-grown plant organs. Furthermore, these extracts may be used as an effective preservative in the food industry.

• 원예과학기술지
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• 제31권5호
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• pp.565-572
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• 2013

본 연구의 목적은 고압나트륨등 광원(HPS)을 조사하여 파프리카의 동적생장과 기관별 건물분배율 및 광이용효율에 미치는 효과를 구명하고자 수행하였다. 파프리카 육묘는 2010년 9월 29일에 $m^2$당 3.75주를 정식하여 2010년 12월 14일부터 HPS 광원을 실험종료일까지 하루 16시간 조사하였다. HPS 처리는 엽수를 증가시켰으나, 절간수와 엽면적에는 미비한 영향을 미쳤다. HPS 처리구의 초장은 대조구에 비해 짧았으나, 줄기당 착과수의 증가를 나타냈다. 줄기와 과실 및 총건물중은 HPS 처리구가 대조구보다 각각 28.5%, 97.1%, 67.8%를 증가시켰다. 총 건물중의 증가율 대비 엽과 줄기 생장율을 비율로 나타낸 각 기관의 건물분배율은 생육초기에 45-47%로 동일하다가 과실의 생장이 시작되는 시점에서 급격히 감소하였다. 영양생장기관으로의 건물분배율은 대조구에서 HPS 처리구보다 4% 높았다. 과실로의 평균 건물 분배율을 HPS 처리구에서 대조구에 비해 14.2% 증가하였다. 동적생장량은 지수선형함수의 최대상대생장율, 최대 절대생장율 및 손실일수로 잘 묘사할 수 있었다. 대조구에 비해 처리구의 엽의 최대절대생장율은 18.6%, 줄기는 74.7%, 과실은 143.5%가 증가하는 것으로 추정되었다. 총 건물중에 대한 광이용효율(LUE)은 HPS 처리구에서 $4.90g{\cdot}MJ^{-1}$로 대조구의 $3.84g{\cdot}MJ^{-1}$ 높았으며, 영양생장기관의 LUE는 HPS 처리구가 $1.56g{\cdot}MJ^{-1}$로 대조구의 $1.61g{\cdot}MJ^{-1}$보다 약간 낮았으며, 과실 생산에 대한 LUE는 처리구가 $3.34g{\cdot}MJ^{-1}$로 대조구 $2.23g{\cdot}MJ^{-1}$보다 49.8% 증가하였다. 처리간 총 건물중의 LUE의 차이는 HPS 처리구의 과실생산에 대한 LUE가 높았기 때문에 발생되었다.

• 한국약용작물학회지
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• 제10권3호
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• pp.212-216
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• 2002

일일초의 vinblastine 함량과 항산화 및 항미생물의 품종별, 부위별 활성을 조사하였다. vinblastine은 식물체내에 극소량 존재하며 9매품종의 잎에서 대부분 검출이 되었다. 특히 Cooler Coconut의 잎에서의 검출량이 $134.08{\mu}g/ml$로 다른 분획물에 비해 10배 가량 높은 수치를 보였다. 항산화 활성 검증에서는 Stardust Orchid의 잎과 Cooler Rose의 잎의 추출물이 각각 $RC50=17,\;18\;{\mu}g/ml$로 항산화 효과가 높음을 알 수 있었다. 부위별로는 대체적으로 잎, 꽃, 뿌리의 순으로 나타났다. 항미생물 활성 검증에서는 모든 품종의 잎이 꽃보다 높은 활성을 보였으며, 특히 Cooler Rose와 Cooler Strawberry가 활성이 좋게 나타났다. 이상의 시험 결과 항산화 활성이 높은 품종이 항미생물 활성에서도 좋은 결과를 보여주었다. 항산화 활성과 항미생물 활성이 높은 품종을 이용하여 앞으로 이를 이용한 여러 약품이나 식품의 개발이 기대된다.

• 한국동물위생학회지
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• 제42권4호
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• pp.275-278
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• 2019

Blindness was observed in five calves born from cattle fed only a commercial feed of growing stage and dried rice straws for about two years in a farm in Gyeongsangbuk-do province. Three of them died within a month after birth, and a body and sera of his mother and other 19 cattle were submitted for diagnosis. At necropsy, the calf was very weak and filled with cerebrospinal fluid in the cerebrum. Any histopathological lesion including atrophy of death of optic nerve cells was not observed, but the irregular proliferation such as lace pattern of choroidal cells and lymphocytic infiltration just below choroid was observed. No pathogen was detected as a result of the etiological tests on the internal organs of calves and bloods. In addition, the levels of serum vitamin A in different affected and his mother cattle were all lower than normal. Finally, we determined this case as an encephalopathy caused by maternal vitamin A deficiency in a calf. This report is an extreme example of how important it is to supply adequate s diets and a good quality of hay for each stage of growth in cattle.

• 문화기술의 융합
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• 제5권3호
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• pp.327-332
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• 2019

현대 과학자들은 식물정화공정과 같은 새로운 기술로 중금속을 제거하려고 한다. 이런 최첨단 기술 중 하나는 토양의 특정 중금속을 제거하는 형질 전환 식물을 개발하는 것이다. 본 연구자는 T. goingense Metal Transport Protein 1 유전자와 TgMTP1 : GFP 유전자를 발현하는 형질 전환 벡터를 구축했다. 형질전환체 식물을 선택하여 형질 전환 된 유전자를 애기 장대 게놈에서 확인했다. 발현은 Arabidopsis 세포, 조직 및 기관의 여러 부분에서 확인되었다. Arabidopsis thaliana에서 TgMTP1 과발현하는 식물에 중금속이온이 처리되었을 때 형질 전환 식물체는 비 형질 전환 체보다 중금속 내성이 높았다. 추가 연구를 위해 4 (Zn, Ni, Co, Cd.)가지 중금속에 대한 내성이 향상된 형질 전환 식물을 선택했다. 선택된 T3 TgMTP1 과다 발현 애기 장대 식물은 중금속에 내성이 증가된다. 이 식물은 액포 내에 중금속을 축적하고 동시에 원형질막에 발현되는 MTP1 유전자의 발현을 특징으로 한다. 결론적으로, 이러한 식물은 식물 정화 응용 분야 및 내성이 증가 된 식물로 사용될 수 있다.