• Journal of Embryo Transfer
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• v.15 no.3
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• pp.271-277
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• 2000

This study was conducted to establish the optimal culture conditions for in vitro production of bovine embryos derived from slaughter house ovaries. Cumulus-oocyte- complexes (COCs) collected by aspiration from follicles of 2~7 mm in diameter were matured in Ham's F-10 medium supplemented with 0.01 $\mu\textrm{g}$/m1 epidermal growth factor (EGF) at 39$^{\circ}C$ in a humidified atmosphere of 5% $CO_2$in air. After 24 hrs of culture, the oocytes were co-cultured with epididymal sperm selected off by Percoll-density gradient in TALP medium for 24 hrs. The presumptive zygotes were cultured in HECM-6 medium for 3 d post-insemination, and followed by cultured in TCM199 medium until 7 to 10d post-insemination. The cultures were compared of their cleavage and development into later stage in culture medium by additions of different protein sources (PVA, BSA and BCS) and by different embryo density. The rates of cleavage and development rates into blastocyst were not significantly (P<0.05) different among the culture media containing with BSA (75.0% and 40.5%), BCS (76.7% and 38.0%) and PVA (72.5% and 42.2%), respectively. Significantly (P<0.05) higher blastocysts rates were obtained in culturing of 30 and 40 embryos in each 50$\mu$l droplets of culture medium than in 5, 10 and 20 embryos. These results indicate that the optimal density of embryos is 30~40 embryos in a 50$\mu$l droplet of culture medium. Furthermore there is no effect of different protein sources on early embryonic development.

• Journal of the Korean Society of Propulsion Engineers
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• v.22 no.6
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• pp.84-93
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• 2018

Three-dimensional (3D) numerical simulations have been carried out to study how coolant injection conditions influence the cooling efficiency and projectile ejection performance in a mixture-gas ejection system (or gas-steam launch system). The 3D single-phase computational model was verified using a 1D model constructed with reference to the previous research and then a two-phase flow computation simulating coolant injection on to hot gas was performed using a DPM (Discrete Phase Model). As a result of varying the coolant flow rate and number of injection holes, cooling efficiency was improved when the number of injection holes were increased. In addition, the change of the coalescence frequency and spatial distribution of coolant droplets caused by the injection condition variation resulted in a change of the droplet diameter, affecting the evaporation rate of coolant. The evaporation was found to be a critical factor in the design optimization of the ejection system by suppressing the pressure drop while the temperature decreases inside the breech.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.12
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• pp.555-562
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• 2019

This study was undertaken to develop a stable self-microemulsifying drug delivery system (SMEDDS) for trans-cinnamaldehyde, a known antibacterial and antifungal agent. A simultaneous analytical method was established for quantification of trans-cinnamaldehyde and its degradant, cinnamic acid. Various surfactants were applied to assess their effect on the aqueous solubility of trans-cinnamaldehyde, and pseudo-ternary phase diagrams were plotted. Of the various formulations tested, the liquid SMEDDS composed of trans-cinnamaldehyde (oil), Cremophor EL (surfactant) and Transcutol P (cosurfactant) at a volume ratio of 10/70/20, produced the smallest emulsion droplet size (around 23 nm). The stability test determined the superior stability of the trans-cinnamaldehyde SMEDDS with constant trans-cinnamaldehyde content and z-average diameter of emulsion, under accelerated and heat stressed condition. Thus, we believe that this novel trans-cinnamaldehyde SMEDDS formulation has the potential to be applied for the development of trans-cinnamaldehyde medicines in the pharmaceutical industry.

• Journal of the Society of Naval Architects of Korea
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• v.35 no.4
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• pp.65-75
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• 1998

The $CO_2$ welding with 100% $CO_2$ gas is commonly used because of its cost and efficiency. Arc phenomena and spattering ratio of the $CO_2$ welding are influenced by various factors such as chemical compositions of welding wire, shielding gas, welding condition and welding power source etc.. Spattering ratio is predominantly influenced by the welding condition which determines a droplet transfer rode. In this study, arc phenomena and spattering ratio are investigated by using two type of FCW(titania type, semi-metal type). Then, the welding quality and optimum welding condition can be selected. From this study, the following results ware obtained; 1) In low current range(140A), FCW up to welding voltage(22V) resulted in a typical short circuit transfer, increase of spattering ratio and growth of spatter diameter. 2) In high current range(320A), the arc stability in titania FCW of a typical globular transfer is better than that of semi-metal FCW.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.12
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• pp.1473-1482
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• 2013

A disc-type rotary atomizer affords advantages such as superior paint transfer efficiency, uniformity of paint pattern and particle size, and less consumption of compressed air compared to a spray-gun-type atomizer. Furthermore, it can be applied to all types of painting materials, and it is suitable for large-scale processes such as car painting. The painting quality, which is closely related to the atomizer performance, is determined by the uniformity and droplet size in accordance with the design of the bell disc surface. This study establishes the basics of how to design a surface by modeling the operating bell disc's RPM, diameter, surface angle, and film thickness considering dye characteristics such as the viscosity, density, and surface affinity.

• Journal of Digital Convergence
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• v.13 no.4
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• pp.417-423
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• 2015

Oil-in-water(o/w) nanoemulsions were prepared in the system of water/Span 80-Tween 80/long-chain paraffin oil via PIC method. With the increase of preparation temperature from 30 oC to 80 oC, the diameter of emulsion droplets decreased from 150 nm to 40 nm. By varying the HLB of mixed surfactants, we found that there was an optimum HLB around 13.0~14.0 corresponding to the minimum droplet size. The size of emulsion droplets increased upon increasing the ratio of oil/emulsifying agent. At $f{\leq}0.15$, the size of nanoemulsions could be kept constant more than 2 months. The increase in preparation temperature makes it possible for producing monodisperse nanoemulsions. Once the nanoemulsion is produced, the stability against Ostwald ripening is outstanding due to the extremely low solubility of the liquid paraffin oil in the continuous phase.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.20 no.2
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• pp.624-631
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• 1996

This study describes extinguishment mechanism of the purely buoyant diffusion flame using the water spray. Experiments are systematically carried out for the oul pool fire with the six different atomizing nozzles. From the measurement of burning rate which represents the combustion intensity of fire, it is observed that the water spray is able to act to enhance fire rather than to extinguish fire. The air entertainment due to the water spray is visualized to understand this phenomenon, acting to enhance fire. In order to observe effects of droplet size on fire extinguishment, and amount of water which reaches the flame base, fuel surface, and mean diameter of droplets are measured. When water droplets are too small, they do not reach the flame base because they can the water spray having too small doplets is ineffective for extinguishment of the oil fire.

• Fire Science and Engineering
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• v.23 no.1
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• pp.48-54
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• 2009

The present study has been performed to investigate the effect of statistical number of droplets on the simulation of the sprinkler spray using fire field model. In order to simulate the sprinkler spray characteristics, the present study uses NIST Fire Dynamics Simulator version 5.2. A group of Lagrangian particles with similar droplet characteristics, such as diameter, velocity, temperature and so on, is represented by parcel concept to decrease the total number of droplets tracked in the simulation. The present study introduces a new parameter to represent the ratio between real number of droplets and computational parcels. The dependency of the number of parcels on the fire suppression characteristics and spray patterns is quantitatively examined for different ratio between the real number of droplets and computational parcels.

• Journal of Adhesion and Interface
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• v.15 no.2
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• pp.57-62
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• 2014

Pythagolas rule was used for investigation of static contact angle in particular figures. Static contact angle measurement was important to evaluate the wettability between solid and liquid. Optimum measurement method and standardization of calculation for static contact angle were investigated for practical application. Optimum diameter of droplet for static contact angle measurement was confirmed as 1 mm. Contact angle measurement using Pythagolas rule was also used to calculate advancing, receding angle and wettability of different surface condition. At last, it was concluded that the Pythagolas rule method was more accurate than general lineation method for static contact angle measurement.

• Journal of Digital Convergence
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• v.10 no.5
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• pp.283-288
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• 2012

This study was undertaken to investigation the spray characteristics of the twin fluid atomization nozzle system. The light oil was injected at the normal temperature and injection pressure was 5 bar - 10 bar by 1 bar and volume flow was 0.5, 1.0 and 2,0 mmH2O(X10-2). We measured the SMD of sprayed droplet to study spray characteristics. The following conclusions were reached from the results of these study. 1. The more injection pressure increased, the more SMD decreased. 2. The more measuring distance between pressure nozzle tip and analyser beam increased, the more SMD increased. 3. SMD of the blower-air-added injection system were shown, increasing volume flow decreased respectively. The result of this study indicated the blower-air-added injection system induced beneficial changes in SMD. And it will be considered important indicator for spray characteristics design and performance evaluation of twin fluid atomization nozzle system.