• Title/Summary/Keyword: diagnostic key

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Development of a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay for the Detection of Haemonchus contortus in Goat Feces

  • Wu, Yao-Dong;Wang, Qi-Qi;Wang, Meng;Elsheikha, Hany M.;Yang, Xin;Hu, Min;Zhu, Xing-Quan;Xu, Min-Jun
    • Parasites, Hosts and Diseases
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    • v.59 no.2
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    • pp.167-171
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    • 2021
  • Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of Haemonchus contortus in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. We compared the performance of the LFS-RPA assay to a PCR assay. The LFS-RPA had a detection limit of 10 fg DNA, which was 10 times less compared to the lowest detection limit obtained by PCR. Out of 24 goat fecal samples, LFS-RPA assay detected H. contortus DNA with 95.8% sensitivity, compared to PCR, 79.1% sensitivity. LFS-RPA assay did not detect DNA from other related helminth species and demonstrated an adequate tolerance to inhibitors present in the goat feces. Taken together, our results suggest that LFS-RPA assay had a high diagnostic accuracy for the rapid detection of H. contortus and merits further evaluation.

Establishment of an Optimal Rehabilitation Process for Agricultural Public Wells by Applying Standardized Diagnostic Functions (농어업용 공공관정 기능진단 표준화(안)을 이용한 최적 사후관리)

  • Lee, Byung Sun;Song, Sung-Ho;Park, Jeong-Keun;Won, Young-Cheon;Kim, Wonsuck
    • Journal of Soil and Groundwater Environment
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    • v.21 no.4
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    • pp.1-9
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    • 2016
  • Rehabilitation for low-yielding wells resulting in improvement on groundwater quantity and quality is considered to be the most economic and ecofriendly method against the increasing demand to groundwater due to frequent drought and the increase in numbers of agricultural complex for growing horticultural crops. This study suggests standard, stepwise diagnostic fuctions consisting of four steps (Basic inspection, Specific inspection, Rehabilitation, and Management) for an optimal management to the wells. Basic inspection can provide information on current groundwater quantity and quality compared with those on its initial stage. Specific-inspection based on hydrogeology can scientifically demonstrate causes of deterioration on groundwater quantity and quality. Results of specific inspection can suggest an optimal rehabilitation method to solve deteriorating problems including clogging and corrosion for the wells. After rehabiliating the wells, an assessment on groundwater quantity and quality would be conducted to identify the suitability of the applied method and improvement of the wells. A short-term, periodic management to the wells is considered as the key to save a public management budget. Suggested diagnostic functions can possibly induce sustainable supply of agricultural groundwater to the farm land and finally contribute the increase on rural household income.

Oriental Pulse Diagnostic Apparatus with an emphasis on sense on fingers (수지부 감각정보에 중점을 둔 한의학적 전자 맥진 시스템에 대한 고찰)

  • Kim, Byoung-Chul;Chae, Han
    • Journal of the Institute of Convergence Signal Processing
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    • v.9 no.2
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    • pp.112-120
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    • 2008
  • This study aims to develop a system for diagnosing pulse and disease conditions based on oriental medical classics to collect objective quantitative information in the traditional pulse examination environment that uses fingers. For this purpose, the study suggested a thimble-type sensor unit as the most appropriate pulse analyzer and proposed a traditional method to measure the pulse pressure wave on the spots of cheok, gwan and chon by installing the pulse analyzer on the forefinger, middle finger and medical finger. Then, it was interpretively found that this pulse analyzer enables us to objectively recognize 28 pulses defined in Oriental medical classics including the yukjo pulse, described as the buchimjisak-heosil pulse, and the hong, yoo, hyeok and san pulses. Finally, the study proposed a method to extract key parameters essential to pulse condition diagnosis from the pulse pressure wave measured by this pulse analyzer.

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Comparision of I-131 Diagnostic Scan and Therapeutic Scan in Thyroid Carcinoma (갑상선암 환자에서 I-131의 진단적 전신스캔과 치료후 전신스캔의 비교)

  • Lee, Bum-Woo;Lee, Dong-Soo;Moon, Dae-Hyuk;Chung, June-Key;Lee, Myung-Chul;Cho, Bo-Youn;Koh, Chang-Soon
    • The Korean Journal of Nuclear Medicine
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    • v.24 no.1
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    • pp.80-86
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    • 1990
  • Fifty seven patients with differentiated thyroid carcinoma were performed radioactive iodine-131 whole body scans after administration of diagnostic dose $(2\sim10\;mCi)$ and therapeutic dose $(30\sim150\;mCi)$ within three months. We evaluated the state of radioactive iodine-131 uptakes in whole body scan to detect correct metastasis of thyroid carcinoma. The results are as follows: 1) In 20 of the 57 patients (35%), the therapeutic scan showed the additional uptakes that were not seen in the diagnostic scan. 2) In 9 (64.2%) of the 14 patients who had been received the thyroid ablation theraphy with I-131 previously, new additional lesions were found in the therapeutic scan but only 11 (25%) of the 32 patients who had not been received the thyroid ablation theraphy disclosed new uptake lesions (p < 0.01). 3) The additional uptake lesions of therapeutic scan were significantly more common in the bony metastatic foci (55.7%) than other areas (p < 0.01). In 11 (55%) of 20 patients, additional uptake regions were anterior neck areas (thyroid bed or regional lymph node). We conclude that diagnostic scan with $2\sim5$ mCi I-131 is inadequate in evaluating residual iodine avid tissues of patients with thyroid carcinoma. Also post-theraphy I-131 whole body scan would be important to evaluate the correct staging and prognosis of thyroid carcinoma, and to follow-up patients.

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Leadership Development in Students as Part of Attitude Development

  • Zhou Yongjun;Viktoriia O. Anishchenko;Olena V. Vasylenko;Nataliia V. Iaremenko;Mykhailo V. Fomin
    • International Journal of Computer Science & Network Security
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    • v.23 no.7
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    • pp.79-90
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    • 2023
  • Leadership development corresponds to the focus on the individual's success and competitiveness strategy. This is the optimal direction of the organization of attitude development because it covers two aspects of the student's personality development, professionally-oriented and self-centric. The aim of the study is to identify and compare the leadership level in second-and fourth-year students to see dynamics of development and implementation of the leadership phenomenon in the professional and personal making up of future specialists. Based on the theoretical analysis of the issue, the authors developed an objective and subjective diagnostic model for leadership skills. In this study, data of the objective diagnostic technique are the key. Subjective diagnostic technique for leadership skills provides insights for problem interpretation. At the level of the first group of respondents, the average Leadership Skills Level of the second-year students was quite low and was found within the medium level. The second group of respondents consisting of the fourth-year students showed a slight but effective improvement. The Leadership Skills of this group were found at a sufficient level. Positive dynamics was revealed for all criteria of leadership skills as a result of applying objective diagnostic methods: decreased percentage of students with negative and relatively low markers of Leadership Skills Level and corresponding increase in percentage of applicants with positive markers of Leadership Skills Level. Further research can be organized in the direction of identifying and developing successful universal and professionally-oriented tactics for leadership development in students as part of attitude development.

Key Technical Challenges for Integrated Sensors in Power Electronics and Motor Drives

  • Lorenz, Robert D.
    • KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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    • v.4B no.4
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    • pp.170-179
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    • 2004
  • The paper presents technical issues which integrated sensors must address to be implemented in the next generation of power electronics and motor drives. The underlying goal of the sensor integration will be to improve reliability of power conversion systems while making the power converter and motor drive become the primary source of diagnostic signals for the application. The paper focuses on design methodologies that will allow this integration to succeed in meeting the technical demands for both reliability and for application level diagnostics.

The Korean Species of the Genus Oxytelus Gravenhorst (Coleoptera: Staphylinidae: Oxytelinae)

  • Lee, Seung-Il;Ahn, Kee-Jeong
    • Animal Systematics, Evolution and Diversity
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    • v.23 no.1
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    • pp.21-33
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    • 2007
  • A taxonomic study of the genus Oxytelus Gravenhorst in Korea is presented. Seven species are recognized, three of which are identified for the first time in Korea (O. incisus Motschulsky, O. migrator Fauvel, and O. takahashii Ito). A key and diagnostic characters with illustrations are provided.

First Record of the Genus Kleidotoma (Insecta: Hymenoptera: Figitidae: Eucoilinae) from Korea

  • Choi, Won-Young;Lee, Jong-Wook;Suh, Kyong-In
    • Animal Systematics, Evolution and Diversity
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    • v.24 no.3
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    • pp.281-284
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    • 2008
  • The genus Kleidotoma (Figitidae: Eucoilinae) is reported for the first time in Korea. We here describe K. striaticollis Cameron (new to Korea) and K. pulchrinis Choi n. sp. A key, descriptions, illustrations of diagnostic characters and distributional data are provided.

First Record of the Genus Spilopteron (Hymenoptera: Ichneumonidae: Acaenitinae) from Korea with Description of a New Species

  • Lee, Jong-Wook;Jeong, Jong-Chul;Lee, Seol-Mae
    • Animal Systematics, Evolution and Diversity
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    • v.24 no.3
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    • pp.275-279
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    • 2008
  • An acaenitine genus, Spilopteron Townes, 1960, is reported for the first time in Korea with S. mucronatus Lee new species. A key to the Korean genera of the tribe Acaenitini Foerster, 1869, description of the a species and photographs of diagnostic characters are provided.

Bronchopulmonary Sequestration - Report of 2 cases and review of 19 cases published in the Korean Journal of Thoracic and Cardiovascular Surgery - (폐격리증 2례 보고 및 대한 흉부외과학회지에 발표된 19례의 문헌 고찰)

  • 곽영태
    • Journal of Chest Surgery
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    • v.20 no.4
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    • pp.829-838
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    • 1987
  • Bronchopulmonary sequestration is a rare congenital malformation of the lung, concerning about the abnormal feeding systemic artery, may happen a serious complication of heeding during operation if not recognized before operation. High index of suspicion of sequestration is the key to successful operative procedure with the aid of characteristic recurrent and long-standing symptoms, its location, and invasive or non-invasive diagnostic tools. We report 2 cases of intralobar type of bronchopulmonary sequestration and review 10 articles about the subject, totaling of 21 cases.

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