• 한국자기공명학회논문지
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• 제17권1호
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• pp.59-66
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• 2013

BldD, a developmental transcription factor from Streptomyces coelicolor, is a homodimeric, DNA-binding protein with 167 amino acids in each subunit. Each monomer consists of two structurally distinct domains, the N-terminal domain (BldD-NTD) responsible for DNA-binding and dimerization and the C-terminal domain (BldD-CTD). In contrast to the BldD-NTD, of which crystal structure has been solved, the BldD-CTD has been characterized neither in structure nor in function. Thus, in terms of structural genomics, structural study of the BldD-CTD has been conducted in solution, and in the present work, mainchain NMR assignments of the recombinant BldD-CTD (residues 80-167 of BldD) could be achieved by a series of heteronuclear multidimensional NMR experiments on a [$^{13}C/^{15}N$]-enriched protein sample. Finally, the secondary structure prediction by CSI and TALOS+ analysis using the assigned chemical shifts data identified a ${\beta}-{\alpha}-{\alpha}-{\beta}-{\alpha}-{\alpha}-{\alpha}$ topology of the domain. The results will provide the most fundamental data for more detailed approach to the atomic structure of the BldD-CTD, which would be essential for entire understanding of the molecular function of BldD.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.89-89
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• 2017

A gene encoding squalene synthase from grain amaranth was cloned and characterized. The full-length cDNA was 1805-bp long and contained a 1248-bp open reading frame encoding a protein of 416 amino acids with a molecular mass of 47.6 kDa. Southern blot analysis revealed that the A. cruentus genome contained a single copy of the gene. Comparison of the cDNA and genomic sequences indicated that the amaranth SQS gene had 12 introns and 13 exons. All of the exons contributed to the coding sequence. The predicted amino acid sequence of the SQS cDNA shared high homology with those of SQSs from several other plants. It contained conserved six domains that are believed to represent crucial regions of the active site. We conducted qRT-PCR analyses to examine the expression pattern of the SQS gene in seeds at different developmental stages and in several tissues. The amaranth SQS gene was low levels of SQS transcripts at the initial stage of seed development, but the levels increased rapidly at the mid-late developmental stages before declining at the late developmental stage. These findings showed that the amaranth SQS is a late-expressed gene that is rapidly expressed at the mid-late stage of seed development. In addition, we observed that the SQS mRNA levels in stems and roots increased rapidly during the four- to six-leaf stage of development. Therefore, our results showed that the expression levels of SQS in stem and root tissues are significantly higher than those in leaf tissues. In present study provides useful information about the molecular characterization of the SQS clone isolated from grain amaranth. Finally, a basic understanding of these characteristics will contribute to further studies on the amaranth SQS.

• 정보관리학회지
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• 제27권2호
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• pp.173-199
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• 2010

이 연구는 어린이도서관의 물리적 공간이 어린이 발달에 영향을 미칠 수 있음을 인식하고 긍정적이고 발전적인 방향으로 어린이 발달을 지원할 수 있도록 평가기준을 수립하는 데 목적을 두고 있다. 이를 위해 먼저 이론적으로 어린이 발달 특성을 검토하고 단계별, 영역별 발달 특성을 적절하게 지원할 수 있는 공간요건의 토대인 이용자편익기준을 분석하였다. 다음으로 이용자편익에서 편익이라는 개념을 구체화하기 위해 어린이도서관 및 어린이공간을 주제로 진행된 선행연구에서 공통적으로 사용되고 있는 편익에 대응되는 개념어들을 추출했다. 그리고 추출된 항목들을 비교분석하고 본 연구의 제시 항목을 추가하여 12개의 개념어를 편익의 대체어로 제시하여 실제 도서관 공간에 적용시켰다. 마지막으로 어린이에게 제공되는 편익 차원에 초점을 맞춘 어린이도서관 공간기준 체크리스트를 제시하였다.

• BMB Reports
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• 제38권2호
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• pp.243-247
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• 2005

Dishevelled (Dvl) is a positive regulator of the canonical Wnt signaling pathway, which regulates the levels of $\beta$-catenin. The $\beta$-catenin oncoprotein depends upon the association of Dvl and Axin proteins through their DIX domains, and its accumulation directs the expression of specific developmental-related genes at the nucleus. Here, the $^1H$, $^{13}C$, and $^{15}N$ resonances of the human Dishevelled 2 DIX domain are assigned using heteronuclear nuclear magnetic resonance (NMR) spectroscopy. In addition, helical and extended elements are identified based on the NMR data. The results establish a structural context for characterizing the actin and phospholipid interactions and binding sites of this novel domain, and provide insights into its role in protein localization to stress fibers and cytoplasmic vesicles during Wnt signaling.

• 한국생활과학회지
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• 제20권1호
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• pp.57-70
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• 2011

The purpose of this study was to analyze the research trends on effortful control with a focus on those found in academic journals. This was done in an attempt to understand the conception of effortful control clearly and lay the foundations for future studies. An analysis was performed on frequencies by years, methodology, and content. Results showed that (1) 95 research articles on effortful control were published between January of 1999 and June of 2010. (2) Most research participants were school-aged children. (3) There were a number of ways that researchers have assessed the concept of effortful control, and the most frequently used measurement scale was the Rothbart's questionnaire for temperament. (4) The main content of the research was the relationships between effortful control and developmental domains (particularly, emotional and social development). More recently, researches examining environmental factors including family and peer environment have increased.

• Child Health Nursing Research
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• 제18권1호
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• pp.36-42
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• 2012

Purpose: The purpose of this study was to explore recognition of mother-child interaction by preschoolers' mothers. Methods: Data were collected through in-depth interviews with mothers of 12 preschoolers. The data were analyzed following Downe-Wamboldt's content analysis methodology. Results: From the data, 170 significant statements were selected and were classified into 7 categories and three domains (maternal, child, dyadic). First, the maternal domain consisted of maternal sensitivity, acceptable responsiveness, and child developmental encouragement. Second, the child domain consisted of child's sensitivity, responsiveness, and initiative. Third, the dyadic domain consisted of mutuality. Conclusion: These results suggest that preschoolers' mothers recognize the significant qualitative aspects of interaction with their children. Based on these results, instruments for preschooler-mother interaction need to be developed.

• International Journal of Computer Science & Network Security
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• 제23권9호
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• pp.91-94
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• 2023

This study aims to evaluate the Higher Diploma in English for the Primary Stage from the diploma students' perspectives. A questionnaire was designed consisting of 25 items distributed in two areas: cognitive/academic preparation and professional/skill preparation. The following statistical analyses were used: means, standard deviations, t-test, and one-way analysis of variance (ANOVA). The study results showed that the level of evaluation of the two domains in the program was low. The study also showed no statistically significant differences between the means of educational diploma students when evaluating the Higher Diploma in English for the Primary Stage due to their academic specialization (Arabic language, social sciences, and Islamic studies). In conclusion, the researcher suggested a developmental mechanism derived from the study results to improve the higher Diploma in English for the Primary Stage.

• 청소년시설환경
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• 제9권4호
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• pp.49-55
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• 2011

The purpose of this study is to investigate and analysis importance for evaluation indicators in youth centers. The sample of it is 138 youth centers. Method of this analysis of importance for its indicators is AHP(Analytic Hierarchy Process). Evaluation domains in youth center are process evaluation, product evaluation, input evaluation and specialized program in order of importance. Also, it is important to evaluate in needs assessment, recruitment of youth instructor, participation of youth in decision making, connective project with schools, sanitation level of youth center, satisfaction research, efforts of improvement of youth center staff in evaluation indicators. The result of this study is that importance for evaluation indicators suggests developmental directivity in youth center management.

• Clinical and Experimental Pediatrics
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• 제50권11호
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• pp.1061-1066
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• 2007

목 적 : 소아의 발달 장애는 조기에 발견하면 조기 치료가 가능하게 되어 치료 효과가 높아지고 후유 장애를 최소화할 수 있다. 발달 장애의 조기 발견을 위하여 기존에 사용해 오던 정식 발달 선별검사는 대부분 시간이 오래 걸리고, 복잡하며, 의료인력이 추가로 필요하기 때문에 바쁜 소아과 외래에서 모든 소아에게 적용하기는 어렵다. 기존의 발달검사 대신에 부모가 작성한 내용을 토대로 평가하는 발달 설문 양식들 중 Ages and Stages Questionnaire (ASQ)는 60개월 이전의 소아의 발달 선별을 목적으로 개발된 검사이다. 본 연구의 목적은 소아과 외래에서 시행하는 발달 지연의 선별 도구로서 ASQ의 타당성을 알아보기 위한 것이다. 방 법 : 가톨릭대학교 의과대학 부속 성모병원 소아과 외래를 방문한 소아의 부모에게 진료 전 대기 시간 동안 한국형 ASQ (K-ASQ)를 완성하도록 하였다. 설문지를 완성한 총 150명 중 만삭 출생아이며 이전에 발달지연의 진단을 받은 적이 없고, 기타 선천성 기형, 경련성 질환을 포함한 신경학적 이상, 유전성 질환의 경력이 없는 소아 67명을 대상으로 하였다. K-ASQ는 각 영역별로 평균에서 2 표준편차(SD) 아래 점수보다 낮으면 "실패"로 판정하며, 한 발달 분야 이상에서 "실패"가 있는 경우를 "선별검사 양성"으로 하였다. 최종 발달평가는 Bayley Scales of Infant Development-II (BSID-II)를 사용하여 판단하였으며, 지능 혹은 운동발달 지수가 평균보다 -1 SD 미만인 경우는 발달지연으로 판정하였다. K-ASQ의 결과를 BSID-II와 비교하여 분석하였다. 결 과 : 1) 대상아의 평균 연령은 $16.4{\pm}7.4$개월($6{\pm}30$개월 범위, 중앙치 14개월)이었다. 10명(14.9%)은 출생 시 부당경량아였으며, 평균 출생체중 $3.1{\pm}0.6kg$, 재태기간 $38.8{\pm}1.4$주이었다. 9명(13.4%)이 쌍생아였고, 33명(49.0%)이 남아였다. 대상 소아들의 모친의 평균 교육 기간은 $13.6{\pm}2.4$년, 53명(79.1%)이 고등학교 졸업 이상의 학력을 가졌고, 21명(31.3%)이 정규직 근무자였다. ASQ 설문지를 완성하는데 평균 $10.2{\pm}3.0$분이 소요되었다. (2) 17명(25.4%)이 ASQ 선별검사 양성이었는데, 그 중 4명은 발달이 지연되었고, 나머지 13명의 발달은 정상이었다. BSID-II로 판정한 발달 지연아 8명 중 4명은 ASQ 선별검사에서 양성이었고, 다른 4명은 선별검사 음성이었다. ASQ 선별검사 음성인 50명 중 4명이 발달지연으로 판단되었다. (3) BSID-II와 비교한 K-ASQ의 민감도(sensitivity)는 50.0%, 특이도(specificity) 78.0%, 양성 예측치 (positive predictive value) 23.5%, 음성 예측치(negative predictive value)는 92.0%로 나타났다. 결 론 : K-ASQ는 높은 음성 예측치를 보여 소아과 외래에서 소아 발달 지연의 선별 도구로서 사용하는 것이 타당한 것으로 판단되었다.

• Interdisciplinary Bio Central
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• 제4권1호
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• pp.1.1-1.7
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• 2012

Background: The F-box proteins represent one of the largest families of proteins in eukaryotes. Apart from being a component of the ubiquitin (Ub)/26 S proteasome pathways, their regulatory roles in other cellular and developmental pathways have also been reported. One interesting feature of the genes encoding the proteins of this particular family is their variable selection patterns across different lineages. This resulted in the presence of lineage specific F-box proteins across different species. Findings: In this study, 48 non-redundant F-box proteins in E. siliculosus have been identified by a homology based approach and classified into three classes based on their variable C-terminal domains. A greater number of the F-box proteins have domains similar to the ones identified in other species. On the other hand, when the proteins having unknown or no C-terminal domain (as predicted by InterProScan) were analyzed, it was found that some of them have the polyglutamine repeats. To gain evolutionary insights on the genes encoding the F-box proteins, their selection patterns were analyzed and a strong positive selection was observed which indicated the adaptation potential of the members of this family. Moreover, four lineage specific F-box genes were found in E. siliculosus with no identified homolog in any other species. Conclusions: This study describes a genome wide in silico analysis of the F-box proteins in E. siliculosus which sheds light on their evolutionary patterns. The results presented in this study provide a strong foundation to select candidate sequences for future functional analysis.