• Title/Summary/Keyword: developed seed

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Development of Gene-based Markers for the Allelic Selection of the Restorer-of-fertility Gene, Rfo, in Radish (Raphanus sativus)

  • Kim, Sunggil;Lim, Heerae;Cho, Kang-Hee;Park, Pue Hee;Park, Suhyung;Sung, Soon-Kee;Oh, Daegeun;Kim, Ki-Taek
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.194-204
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    • 2009
  • Cytoplasmic male sterility (CMS) and fertility restoration have been utilized as valuable tools for $F_1$-hybrid seed production in many crops despite laborious breeding processes. Molecular markers for the selection of CMS-related genes help reduce the expenses and breeding times. A previously reported genomic region containing the Ppr-B gene, which is responsible for restoration of fertility and corresponds to the Rfo locus, was used to develop gene-based or so-called "functional" markers for allelic selection of the restorer-of-fertility gene (Rfo) in $F_1$-hybrid breeding of radish (Raphanus sativus L.) Polymorphic sequences among Rfo alleles of diverse breeding lines of radish were examined by sequencing the Ppr-B alleles. However, presence of Ppr-B homolog, designated as Ppr-D, interferes on specific PCR amplification of Ppr-B in certain breeding lines. The organization of Ppr-D, resolved by genome walking, revealed extended homology with Ppr-B even in the promoter region. Interestingly, PCR amplification of Ppr-D was repeatedly unsuccessful in certain breeding lines implying the lack of Ppr-D in these radishes. Ppr-B could only be successfully amplified for analysis through designing primers based on the sequences unique to Ppr-B that exclude interference from Ppr-D gene. Four variants of Rfo alleles were identified from 20 breeding lines. A combination of three molecular markers was developed in order to genotype the Rfo locus based on polymorphisms among four different variants. These markers will be useful in facilitating $F_1$-hybrid cultivar development in radish.

Effect of Three Microalgal Species on Gonadal Development and Sex Maturation of Ark Shell, Scapharca broughtonii (미세조류 3종에 따른 피조개 Scapharca broughtonii의 생식소 발달과 성숙)

  • Min, Byeong-Hee;Kim, Byeong-Hak;Kim, Sung-Yeon;Shin, Yun-Kyung;Hur, Sung-Bum
    • The Korean Journal of Malacology
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    • v.27 no.2
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    • pp.143-148
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    • 2011
  • Even though ark shell, Scapharca broughtonii is commercially important species, its artificial seed production is not yet fully developed. This study was carried out to understand the effect of three microalgal species on gonadal development and sex maturation of S. broughtonii in terms of broodstock management and food organism. Isochrysis galbana, Phaeodactylum tricornutum and Tetraselmis tetrathele were supplied to S. broughtonii broodstock in single or mixed. And condition index, gonadal development, sexual maturation and survival of the broodstock were analysed. After 45 rearing days, frequency of ripe stage of gonadal phases, rate of induced sexual maturation and survival of S. broughtonii broodstock fed mixed diet with 3 microalgal species or single diet of T. tetrathele were the highest.

Morphology and Characteristics of Floral Organ in Highbush Blueberry (Vaccinium corymbosum) Cultivars (하이부쉬 블루베리의 품종별 화기 특성 및 형태적 관찰)

  • Kim, Su Jin;Bae, Kang Soon;Koh, Sang-Wook;Kim, Ho Cheol;Kim, Tae-Choon
    • Korean Journal of Plant Resources
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    • v.28 no.2
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    • pp.235-242
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    • 2015
  • Morphology and characteristics of floral organ in highbush blueberry cultivars were studied to select suitable cultivars of highbush blueberry for domestic cultivation. The stamen consists an anther and a tape-like hairy filament with well-developed trichomes. When the anther was opened, the wall of anther was not dehiscent, and pollen grains were discharged into two tubes. Pollen was mature tetrad type without being separated after meiosis (Late March). The number of pollen granules per anther was 400~1,300, the germination rate was higher in the cultivars having many pollen grains. Pistil was composed of five carpels and a shipper without separate part. The number of ovules per ovary was 39~67, therefore, the coefficient of ranged from 11.6 to 31.0%. The seed pod formation by combination of ‘Bluejay’ and ‘Sharpblue’ was higher in the cross-pollination than in the self-pollination.

Generation of Design Time History Complying With Japanese Seismic Design Standards for Nuclear Power Plants (일본 원전 내진설계 기술기준을 적용한 모의지진파(가속 도시간이력) 작성)

  • Gin, Seungmin;Kim, Yongbog;Lee, Yongsun;Moon, Il Hwan
    • Journal of the Earthquake Engineering Society of Korea
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    • v.25 no.2
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    • pp.83-91
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    • 2021
  • Seismic designs for Korean nuclear power plants (NPPs) under earthquakes' design basis are noticed due to the recent earthquake events in Korea and Japan. Japan has developed the technologies and experiences of the NPPs through theoretical research and experimental verification with extensively accumulated measurement data. This paper describes the main features of the design-time history complying with the Japanese seismic design standard. Proper seed motions in the earthquake catalog are used to generate one set of design time histories. A magnitude and epicentral distance specify the amplitude envelope function configuring the shape of the earthquake. Cumulative velocity response spectral values of the design time histories are compared and checked to the target response spectra. Spectral accelerations of the time histories and the multiple-damping target response spectra are also checked to exceed. The generated design time histories are input to the reactor building seismic analyses with fixed-base boundary conditions to calculate the seismic responses. Another set of design time histories is generated to comply with Korean seismic design procedures for NPPs and used for seismic input motions to the same reactor containment building seismic analyses. The responses at the dome apex of the building are compared and analyzed. The generated design time histories will be also applied to subsequent seismic analyses of other Korean standard NPP structures.

Fruit Yield and Quality Evaluation of Sweet Pepper (Capsicum annuum L.) F1 Hybrids Derived from Inbred Lines

  • Shrestha, Surendra Lal;Luitel, Binod Prasad;Lee, Taek Jong;Kang, Won Hee
    • Korean Journal of Breeding Science
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    • v.42 no.4
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    • pp.344-350
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    • 2010
  • Sweet pepper inbred lines (KNU1003, KNU1006, KNU1007, KNU1009, KNU1015, KNU1017 and KNU2006) developed at Kangwon National University (KNU) through conventional means, inbred lines (5AVS1, 5AVS2, 5AVS3, 5AVS5, 5AVS7 and 5AVS8) collected at Rural Development Administration (RDA) and inbred lines (SP12, SP27 and SP14) derived from anther culture were used as female parents and anther culture derived homozygous lines (SP9, SP10, SP14, SP24, SP25, SP27, SP30, SP32, SP34, SP38, SP43, SP45 and SP51) were used as male parents to produce $F_1$ hybrids. A total of 37 $F_1$ hybrids were evaluated for fruit yield and quality characters in summer season, 2007. Variation in fruit number, fruit weight, fruit yield per plant and fruit volume was observed among the $F_1$ hybrids. Superiority on yield over standard/commercial varieties were differed among $F_1$ hybrids. Hybrid $5AVS8{\times}SP45$ exhibited highest heterosis over Special (16.5%) and Fiesta (24.7%). Fruit quality characters (fruit length, fruit width, pericarp thickness, total soluble solid, fruit shape and fruit color) were varied among the $F_1$ hybrids. Fruit number, fruit weight and fruit volume per plant were correlated with fruit yield. Based on the standard heterosis expressed by the hybrids and quality characters evaluation, $KNU1017{\times}SP27$, $5AVS1{\times}SP43$, $5AVS5{\times}SP27$, $5AVS8{\times}SP45$, $SP12{\times}SP38$ and $SP27{\times}SP25$ hybrids were found to be superior over commercial cultivars and are selected. Inbred lines of these hybrid combinations can be used to produce $F_1$ hybrid seed for commercial production.

Characterization of a New High-lysine Mutant in Barley (Hordeum vulgare L.)

  • Kim, Hong-Sik;Kim, Dea-Wook;Kim, Sun-Lim;Baek, Seong-Bum;Park, Hyoung-Ho;Hwang, Jong-Jin;Kim, Si-Ju
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.375-382
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    • 2011
  • A chemical, MNU-induced hulless barley mutant line designated as 'Mutant 98 (M98)' was developed from a Korean hulless waxy barley cultivar, 'Chalssalbori'. The objective of the study was to determine the genetic basis of 'M98' and the possibility of using 'M98' as breeding parent to improve lysine level. Compared to 'Chalssalbori', 'M98' had large embryo and higher lysine content in both the embryo and endosperm. Significantly different lysine content in 'M98' and the other high-lysine barley mutant stocks was observed for two years. However, the genotype by year interaction was not significant. 'M98' was higher than the other high-lysine barley mutant stocks in the percentage of lysine of total amino acid composition (0.75%). The trait of shrunken endosperm of 'M98', which was typical in the high-lysine mutants, was inherited by a single recessive gene. Based on seed morphology and lysine content of $F_1$ seeds, 'M98' had a genetically different gene from the other high-lysine mutants for shrunken endosperm. Segregation of $F_2$ for plump/shrunken endosperm did not fit the expected ratio of Mendelian inheritance except for only one cross combination (GSHO1784 (lys1)/M98). The amino acid analysis of $F_5$ and $F_6$ progenies from the cross between 'M98' and 'Chalssalbori' revealed that the attempt to increase the range of lysine content of plump lines did not go beyond the limit of the average high-lysine barley germplasm.

Application of Near-Infrared Reflectance Spectroscopy to Rapid Determination of Seed Fatty Acids in Foxtail Millet (Setaria italica (L.) P. Beauv) Germplasm

  • Lee, Young Yi;Kim, Jung Bong;Lee, Sok Young;Lee, Ho Sun;Gwag, Jae Gyun;Kim, Chung Kon;Lee, Yong Beom
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.448-454
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    • 2010
  • The objective of this study was to rapidly evaluate fatty acids in a collection of foxtail millet (Setaria italica (L.) P. Beauv) of different origins so that this information could be disseminated to breeders to advance germplasm use and breeding. To develop the calibration equations for rapid and nondestructive evaluation of fatty acid content, near-infrared reflectance spectroscopy (NIRs) spectra (1104-2494 nm) of samples ground into flour (n=100) were obtained using a dispersive spectrometer. A modified partial least-squares model was developed to predict each component. For foxtail millet germplasm, our models returned coefficients of determination ($R^2$) of 0.91, 0.89, 0.98 and 0.98 for strearic acid, oleic acid, linoleic acid, and total fatty acids, respectively. The prediction of the external validation set (n=10) showed significant correlation between references values and NIRs values ($r^2=0.97$, 0.91, 0.99 for oleic, linoleic, and total fatty acids, respectively). Standard deviation/standard error of cross-validation (SD/SECV) values were greater than 3 (3.11, 5.45, and 7.50 for oleic, linoleic, and total fatty acids, respectively). These results indicate that these NIRs equations are functional for the mass screening and rapid quantification of the oleic, linolenic, and total fatty acids characterizing foxtail millet germplasm. Among the samples, IT153491 showed an especially high content of fatty acids ($84.06mg\;g^{-1}$), whereas IT188096 had a very low content ($29.92mg\;g^{-1}$).

A Study on the Development and Consumer Preference of the Soup·Stew HMR New Products (탕·찌개류 HMR 신제품 개발을 위한 소비자 기호도 연구)

  • Lee, Seung-Min;Choi, Eun-Kyoung;Cho, Mi-Sook;Oh, Ji-Eun
    • The Journal of the Korea Contents Association
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    • v.19 no.8
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    • pp.123-136
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    • 2019
  • This paper is a study on the development and consumer preference of the soup stew HMR new products, and the main contents are as follows. A total of nine products were studied, including three developed products and six market products. For this study, the demographic characteristics, the awareness and attitude of HMR products were investigated. In addition, the consumer panel evaluated overall liking, appearance, color, flavor, and taste using the nine-point recertification scale, while the strength assessed viscosity, sweetness, saltiness, sourness, and Umami. Familiarity, health degree, Purchase intention and Recommendation intention were investigated and the reasons for preference and non preference were analyzed by multi-response method. The consumer preference analysis indicates that the product is competitive among existing products and that it would be desirable to improve after identifying the causes of non-preferred factors.

Effect of pulverizing method on the particle size of matured silkworm powder

  • Kim, Su-Bae;Kim, Kee-Young;Ji, Sang-Deok;Kim, Seong-Wan;Kim, Nam-Suk;Jo, You-Young;Kim, Jong-Gil;Kim, Young-Guk;Koo, Hui-Yeon;Moon, Hyung-Chul;Seok, Young-Seek;Lee, Hyun-Tai
    • International Journal of Industrial Entomology and Biomaterials
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    • v.37 no.2
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    • pp.105-108
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    • 2018
  • Recently matured silkworm powder was developed by RDA. In this study, the effect of pulverizing method on the particle size of matured silkworm powder was examined. FESEM was performed to observe the morphology and to measure the particle size of silkworm powder. Particle morphology of air-jet mill pulverized powder was round and smooth, however, those of roller-mill and hammer-mill pulverized mature silkworm was more harsh and square. Particle size was varied with pulverizing technique as follows; $1.1{\mu}m$ (air-jet mill), $10{\mu}m$ (roller mill), and $120{\mu}m$ (hammer mill), respectively. A proximate analysis results of air-jet mill powder showed that crude protein, crude lipid, crude fiber, and ash was 73%, 12%, 1.95%, and 3.4%, respectively. According to our results, air-jet mell technique might be used to make a tiny matured silkworm powder.

First Report of Black Rot Caused by Diplodia seriata on Apple (Diplodia seriata에 의한 사과 검은썩음병 발생보고)

  • Kim, Young Soo;Yun, Yun Joo;Jeon, Yongho
    • Research in Plant Disease
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    • v.24 no.4
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    • pp.321-327
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    • 2018
  • In 2018, symptoms of black rot on apple (Malus pumila var. dulcissima KOIDZ) cv. Fuji were observed in Yeongcheon-si, Korea. The fruit decay symptoms consisted of purple pimples spots, black rot around the seed cavity (calyx end), mummified fruit. To isolate the causal agent, small fragment (2 to 3 mm) of decayed tissue from the lesion margin were placed on WA or PDA. Fungal colonies on PDA produced dense white aerial mycelium, becoming dark gray with age. Pycnidia and conidia were observed under a light microscopy. The shapes of conidia were aseptate, ovoid, rounded at both ends, and $21.7-28.3{\times}9.9-15.3{\mu}m$. Based on morphological and cultural characteristics, this fungus was identified as Diplodia seriata. To confirm its identity, two loci (ITS and ${\beta}$-tubulin) were sequenced for molecular identification. BLAST searches indicated 100% identity with D. seriata. A pathogenicity test was conducted with isolates on Fuji apples. The apples were inoculated with mycelial plugs (5 mm) from 7-day-old cultures of the putative pathogens. All inoculated apples developed rot symptoms identical to the original symptoms, from which D. seriata were reisolated, fulfilling Koch's postulates. This study is the first report of black rot caused Diplodia seriata on apple.