• Korean Journal of Agricultural Science
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• v.48 no.4
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• pp.729-738
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• 2021

The purpose of this study was to suggest an improvement plan ultimately to develop practical evaluation methods for national technical qualifications in the field of seeds and horticulture based on the National Competency Standards. Through this, national technical qualifications can be strengthened in terms of professionalism in the seeds and horticulture field. In order to conduct this study, the national technical qualification currently in use was matched to certain competency units and competency unit elements of National Competency Standards. We then visited an industrial site to understand the practical factors related to the technical, facility and equipment aspects of the seeds and horticulture industrial field, after which a practical evaluation exam was developed. Also, for a feasibility study, a pilot test assessment was conducted for students majoring in seeds and horticulture, with a survey also conducted with field experts and an advisory committee consisting of experts in seeds and horticulture. Based on the results, by suggesting an improvement plan for a practical evaluation method leading to better national technical qualifications in the field of seeds and horticulture, it was shown to be possible to improve knowledge, technical, and literacy aspects. This can lead to the training of outstanding technical professionals. Ultimately, the results here are expected to contribute to the improvement of the seeds and horticulture industry.

• Women's Health Nursing
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• v.6 no.3
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• pp.372-382
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• 2000

The purpose of this quasi-experimental study was to explore the differences in the effect of aromatherapy according to the type of Sasang constitution. The subjects were fifty-three nursing students experiencing clinical practice from March 10th. 2000 to April 22nd, 2000. For experiment, researcher used inhalation of 2% grape seed oil mixed with Rosemary oil for 2 minutes and massage with same oil for 20 minutes on the back of subjects in the speed of 20 strokes per minutes. The instruments used for this study were Questionnaire of Sasang Constitution Class II program for personal computer developed by Kim Sun Ho et al., (1996), and Mood Questionnaire developed by Ryman. Biersner, & Larocco (1974). The concentration of IgA within saliva was analyzed by immunoturbidimeter assay. The data were analyzed using SPSS and hypotheses were examined with paired t-test and ANCOVA. The results were as follows : 1) The mean score of mood was increased significantly after aromatherapy. 2) The mean concentration of salivary IgA was increased significantly after aroma therapy. 3) There was no significant difference in the mean score of mood after aromatherapy according to the type of Sasang constitution, 4) There was no significant difference in the mean concentration of salivary IgA after aromatherapy according to the type of Sasang constitution. In conclusion, the results suggest that aromatherapy with Rosemary oil have effect on the improvement of mood and IgA within saliva in the nursing students under stress caused by clinical practice regardless of the type of Sasang constitution.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.2
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• pp.43-52
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• 1999

This study was carried out to verify results of the nursery seedbeds. From November of 1997 to September of 1998, the artificial banking slopes in the greenhouse of the College of Agriculture and Life Sciences, Seoul National University were seeded with the mixtures of those species. Most of exotic species showed relatively poor development of root as short as 30cm. Also the green weight of root biomass of the native species was more than two times than that of the exotic species. On the other hand, it was found that the exotic species have relatively well-developed fine roots. Thus, it was concluded that the seed-mixture of the native species with long and thick roots and the exotic species with fine roots be the most effective method for topsoil erosion control on banking-slopes. The artificial rainfall system treatment(30mm/hr, 60mm/hr, 100mm/hr) on $30^{\circ}$ banking-slopes did not cause any significant change in the amount of soil loss by erosion. The root system was best developed in the plot of 1,000 seedlings per square meter and it performed well for soil erosion control. Consequently, in the case of seeding of single herbaceous species without mixing any woody seeds, the expected seedlings were 1,000 to 2,000 per square meter.

• Journal of the Korean Ceramic Society
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• v.31 no.9
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• pp.1076-1086
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• 1994

This study covers synthetic effect of the various hydrothermal treatments on formation of artificially made kaolinite mineral. The hydrothermal treatment includes the temperature treatment with time duration, addition of seeds, particle size of the starting material used, pH variation and the different types of organic acids. A colloidal silica and alumina sol which are commercially available are used for this study. A colloidal silica and alumina sol are mixed by the atomic ratio of Al/Si = 1, based on the theoretical kaolinite composition and calcined at $600^{\circ}C$ for 8 hours duration. It was found that the kaolinitic clay mineral was well developed; thereby, the different patterns of crystalline mineral are appeared. Spherical type as a crystal form was distinctively formed at the temperature of 20$0^{\circ}C$ to 25$0^{\circ}C$ with short duration time, while platy type as a crystal was highly yielded at 300~35$0^{\circ}C$. Moreover, by adding more than 20 wt% of seed as the natural kaolinitic clay to the starting material is widely distributed and developed when 2 ${\mu}{\textrm}{m}$ or less particle size of the starting material is used; also, when they are heat-treated at the temperature of 25$0^{\circ}C$ with 5 hours duration. With respect of the effect of pH variation on formation of the synthetic kaolinite minerals, the crystalline minerals are highly yielded at less than pH 2 and gradually diminished at more than pH9. Regarding to the effect of different acids on development of the kaolinite mineral, the organic acids with high chelating capacity produces good formation of crystalline minerals; whereas, amine radical-(NH2) is not an effective agent to generate the crystalline minerals.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.6
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• pp.531-536
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• 1993

Bulbosum method, which haploids are developed by pollinating with H. bulbosum have been productive to be recommended as an effective method for the production of genetically stable normal barley hybrids. The purpose of this study is to investigate several characters of H. bulbosum, seed set and embryo development rate pollinated with H. bulbosum in order to establish a method for improvement of embryo development and to find conditions favoring plant development from the embryos cultured in vitro. Three lines of H. bulbosum used as pollinators: GBC(2${\times}$), Spenish diploid(2${\times}$) and var. Jaaska (4${\times}$) were morphologically similar, having characteristically narrow leaves, narrow and long culms, long spikes and anthesis duration in comparison with H. vulgare. H. bulbosum, var. Jaaska(4${\times}$) on being pollinated to barley cultivars, increased embryo formation by 8% and plant development in vitro by three times compared to the other diploid lines. the plants developed were not normal barley hybrids but had some H. bulbosum chromosomes uneliminated, indicating that the line was unstable as a pollinator to induce barley haploids.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.1
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• pp.86-94
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• 1999

A set of rice recombinant inbred lines was developed from a cross between a Tongil type variety, Nonganbyeo, and an indica variety, BG276, by the single seed descent method. The number of the lines in the population was 272. All the agronomic characters studied except ADV (alkali-digestion value) showed continuous variation among the RILs, implying that their inheritance mode should be quantitative. The patterns of the variation in the RILs were either normal or skewed distribution. ADVs of RILs were segregated into two groups with 1:1 ratio, indicating that ADVs in this KIL population might be controlled by one major gene. Transgressive variations were also observed in all characters. Heritability values of the characters varied from 0.488 in brown/rough rice ratio to 0.895 in alkali-digestion value. In the analysis of genotypic and phenotypic correlations, the character of yield was positively correlated with 8 different agronomic characters. The number of panicles per hill was negatively correlated with culm length, panicle length, and number of spikelets per panicle. Grain length was positively correlated with grain width, grain thickness, grain length/width ratio, white belly, ADV, and amylose. However, grain length/width ratio was negatively correlated with grain width. White core was also negatively correlated with white belly and ADV.

• Research in Plant Disease
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• v.19 no.3
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• pp.220-225
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• 2013

For quarantine purpose, we developed the RT- and nested PCR module of Tomato black ring virus (TBRV), Arabis mosaic virus (ArMV), Cherry leafroll virus (CLRV) and Grapevine fanleaf virus (GFLV). The PCR modules, developed in this study make diagnosis more convenient and speedy because of same PCR condition. And also, the methods are more accurate because it can check whether the result is contamination or not using the mutation-positive control. We discard or return the 27 cases of Nepovirus infection seed by employing the module past 3 years. This study provides a rapid and useful method for detection of four quarantine plant viruses.

• Korean Journal of Medicinal Crop Science
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• v.11 no.2
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• pp.122-126
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• 2003

This study was conducted to investigate plant growth characteristics and anatomical studies of Korean mistel (Viscum album var. coloratum (Kom.) Ohwi). Parasite position was upper part that has a plenty of sunshine area 5 to 10m apart from the ground, regardless host tree. Stem developed two branch stems from the each basal stem, leaf was grown from the end stems as opposite. Fruit has a light yellow color and berry size was 7 to 8 mm long diameter and it contains seed. Floral bud differentiation initiated with forming rounded dome and arch-shaped floral apex at the beginning stage, and produced heart-shaped sepal following the differentiation of leaf primordia. Floral bud formation processed centeral part first and following bilateral sides were developed in order. Leaf was thick and stoma and guard cell were observed from the two or three inner layers from the epidermis. Mistletoe penetrated cork cambium of host plant and was growing by rooting on xylem. Korean mistel connected to host plant through parasitic organ, haustorium structurally and physiologically.

• Journal of Crop Science and Biotechnology
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• v.10 no.1
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• pp.13-18
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• 2007

Near-infrared spectroscopy(NIRS) was used as a rapid and nondestructive method to determine the fatty acid composition in intact seed samples of rapeseed(Brassica napus L.). A total of 349 samples(about 2 g of intact seeds) were scanned in the reflectance mode of a scanning monochromator, and the reference values for fatty acid composition were measured by gas-liquid chromatography. Calibration equations for individual fatty acids were developed using the regression method of modified partial least-squares with internal cross validation(n=249). The equations had low SECV(standard errors of cross-validation), and high $R^2$(coefficient of determination in calibration) values(>0.8) except for palmitic and eicosenoic acid. Prediction of an external validation set(n=100) showed significant correlation between reference values and NIRS estimated values based on the SEP(standard error of prediction), $r^2$(coefficient of determination in prediction), and the ratio of standard deviation(SD) of reference data to SEP. The models developed in this study had relatively higher values(> 3.0 and 0.9, respectively) of SD/SEP(C) and $r^2$ for oleic, linoleic, and erucic acid, characterizing those equations as having good quantitative information. The results indicated that NIRS could be used to rapidly determine the fatty acid composition in rapeseed seeds in the breeding programs for high quality rapeseed oil.

• KSBB Journal
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• v.24 no.2
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• pp.170-176
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• 2009

Lectin was finally isolated on Sephadex G-100 from Korean soybean cultivars developed for soy source and investigated its some biochemical properties. Native PAGE pattern of this lectin revealed a molecular weight of 108 kDa as tetramer. The molecular weight of this lectin isolated as double protein band by SDS-PAGE was calculated to be 32 and 22 kDa from the relative mobilities compared with those of the standard proteins. Among the tested red blood cell, the isolated lectin agglutinated rabbit red blood cell treated with trypsin, but did not agglutinated human red blood cells (A, B, AB, O), rat, and untreated rabbit red blood cell. The optimal temperature and thermal stability of isolated lectin was at 20-$50^{\circ}C$ and 10-$60^{\circ}C$, respectively. This lectin was stable at 7.2, and showed complete loss in its activity below pH 6.2 and above pH 8.0.