• Journal of Plant Biology
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• v.36 no.4
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• pp.377-382
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• 1993

Rice (Oryza saliva L.) calli containing both embryogenic callus (EC) and non embryogenic callus (NEC) regions were initiated from the mature seed on MS medium supplemented with 2.0 mg/L 2,4-D, 0.5 mg/L kinetin. The calli were developed into two callus type which can be distinguished by visual examination depending on color and appearance. In order to illucidate the polypeptide composition between EC and NEC, the total protein extracted from two types of callus was analysed by electrophoresis. By one-dimesional anlaysis of SDS-PAGE and Isoelectric focusing, several protein bands showed quantitative and qualitative difference in each type of callus. The further analysis of the total protein with two-dimensional electrophoresis showed at least 20 EC specific protein and 10 NE specific protein. Also 3 specific protein spots showing micro heterogeneity of 90, 65, 50 kD were detected in EC, while a series of acidic heterologous protein spots were visualized in NEC.in NEC.

• Korean Journal Plant Pathology
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• v.11 no.4
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• pp.292-297
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• 1995

We developed an in vitro assay method for evaluating plant growth promotion and providing an evidence that the growth promotion is rendered by growth enhancing factors. The amendment of culture filtrates of Trichoderma harzianum T95 and Gliocladium virens G872 and G872B in Murashige and Skoog (MS) agar medium enhanced the cotyledon growth of cucumber in terms of fresh weight and primary leaf area of cucumber cotyledon cuttings, of which the treatment of G. virens G872B was the most effective. The mycelial culture filtrate of G872B was more effective in the growth promotion than its conidial germling filtrate. The addition of 1% sucrose in MS mineral medium with 0.1% culture filtrates of the antagonists (T95 and G872B) was optimum for enhancing the effect of the filtrates on the growth of cotyledon cuttings in vitro. When cucumber seeds treated with G872B, Pseudomonas putida Pf3 or the G872B-Pf3 mixture were planted in a greenhouse, the rate of seed germination, biomass of shoot and root, and yield of cucumber fruits were increased, especially by G872B or the G872B-Pf3 mixture. Correspondingly, cucumber fruit yields in early to middle-cycles of harvest were significantly greater in the plots of G872B than the control and Pf3-treated plots, and the final yield was highest in the plots of the G872B-Pf3 mixture applications.

• Journal of the Korean Society for Precision Engineering
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• v.26 no.3
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• pp.137-143
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• 2009

It has been reported that mechanical stimulation takes a role in improving eel/ growth in skeletal system. Various research groups have been showed their own bioreactors which stimulate cell-seed three-dimensional scaffold. In this study, we hypothesized that the various conditions of mechanical stimulation would affect cell growth and proliferation. To prove our hypothesis, we designed a custom-made bioreactor capable of applying controlled compression to cell-encapsulated scaffolds. This device consisted of a circulation system and a compression system. Each parts of the bioreactor was fabricated using the rapid prototyping technology By using the rapid prototyping technology, we can modify and improve the bioreactor very rapidly For dynamic cell-culture, cell-encapsulated agarose gel was fabricated in 2% concentration. We performed dynamic cell-culture using this agarose gel and developed bioreactor in 3 days.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.10
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• pp.3942-3949
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• 2010

Since existing Nate-on Messenger application stores users' personal information in the database of its server, it is extremely venerable to internal threats, not to mention the communication data being transmitted without any safety measures. To solve such problematic areas of the existing application, we have developed a safer messenger application. The messenger application proposed in this paper discloses only the least required personal information of its users and the rest of the personal information is safely encrypted in the database using private passwords. This protective measure prevents the administrator or a third party from misusing the information since he/she will not be able access the information. In addition, users will be able to freely and safely communicate using this new messenger since transmitted data will also be encrypted.

• Journal of fish pathology
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• v.6 no.2
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• pp.197-204
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• 1993

Flounder culture has been developed mainly in the western parts of japan, and, to date, following six bacterial diseases have been reported. Bacterial white enteritis occurs in 16 to 30-day-old flounder larvae and often causes mass mortality in seed production. Bacterium named Vibrio sp. INFL invades and multiplies in the mucosae of posterier part of intestine, and causes desquamative enteritis. Gliding bacterial disease occurs mostly in juvenile stage and in spring to summer. Diseased signs are partial discoloration and erosion of skin and fins. Histologically, epidermis are removed, and the causative bacterium, Flexibacter maritimus, multiplies on the surface of demis and invades into the muscular tissue. Vibriosis caused by Vibrio anguillarum and related organisum is one of the well-known diseases among marine fish. Outbreaks of the disease in flounder culture are relatively few, but mass mortalities in fingerlings due to the disease were reported. An outbreak of nocardiosis in the autumn of 1984 has been reported, but since then the disease scarcely occurred. The disease is characterized by formation of abscesses under the skin and white nodes in the gill, heart, spleen and kidney. Streptococcicosis occurs frequently in recent years. Beta-hemolytic streptococcus is the causative bacterium, which possesses the same biochemical and serological characteristics as $\beta$-streptococci isolated from some marine and freshwater fish, and is seemed to related to Streptococcus iniae. Edwardsiellosis is the disease that causes most damage in flounder culture in Japan. Characteristic symptoms are swelling of abdomen and intestinal protrusion from the anus due to accumulation of ascites. Edwardsiella tarda, a well-known pathogen of freshwater fish, is the causative bacterium of the disease.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.3
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• pp.495-508
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• 1994

A backcalculation procedure to determine the layer moduli of flexible pavement structure is developed using matrix solution technique. Forward calculation computer program adopted in this backcalculation procedure is SINELA which is one of layered elastic computer programs. Data base system is used as a tool for setting initial seed moduli and depth to virtual bedrock in backcalculation procedure. The validity and applicability of the proposed backcalculation procedure are verified through various numerical model tests. From the results of comparison analysis with FPEDD1, it is found that the proposed procedure gives more efficient and accurate results.

• Journal of The Korea Institute of Healthcare Architecture
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• v.21 no.3
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• pp.57-67
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• 2015

Purpose: As the concerns for the environment are gradually prioritized, increasing interests of environment-friendly buildings are drawn. Numerous researches about healthcare buildings have been performed however, they were mainly focused on convenience or healing condition for medical treatments. The hospitals consume energy and generate $CO_2$ as twice as the residential or commercial buildings do. Various green building certification systems are globally arranged. But, it isn't easy to find certification criteria for the green hospital besides the US and UK's ones which are specially designed to evaluate environment-friendly medical buildings(Lim&Yoo, 2010). Methods: In this study, we investigates current conditions of Tokyo Regional hospitals in environment-friendly standpoint and also investigates the foreign certification criteria and systems for the green healthcare building and finds the possibility to apply them to Korean one. Results: Through this study, we found that the Tokyo regional hospitals can be environmentally improved by management plans and programs. Implications: Based on this analysis, Korean Green Building Certification for healthcare facilities will be developed in near future.

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.2
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• pp.115-123
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• 2003

Oviposition and egg laying is an important physiological and behavioural event in the life cycle of the silkworm, Bombyx mori (L). Oviposition and egg laying is dependent on a number of intrinsic and extrinsic factors viz., neural, hormonal, environmental, physical, behavioral etc for the perpetuation of population. Although, the virgin female moths have fully developed embryos but active egg laying begins under the influence of mating which provides essential copulation stimulus for oviposition. After mating drastic biochemical changes occurred that incites egg laying under the influence of optimum environmental conditions. Weight of pupae as well as larval density has significant role on oviposition and egg laying behaviour in the silkworm wherein high pupal weight and inadequate rearing space affects not only the biology, morphology and physiology but also the oviposition and egg laying. Surface topography, plane of inclination, mating length, age of male moth, temperature and photoperiod etc has significant effect on reproductive physiology of silkworm. An attempt has been made in this review article to elucidate briefly the works carried out on mating behaviour, direct and indirect copulation stimulus, vitellogenesis, influence of environmental factors besides effect of weight of pupae and or pharate adult and larval density on oviposition and egg laying behavior in the silkworm, B. mori and its significance in silkworm seed production.

• Journal of Microbiology and Biotechnology
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• v.15 no.4
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• pp.701-709
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• 2005

An efficient method of selecting an antagonistic strain for use as a biological control agent strain was developed. In this improved method, the surface tension reduction potential of an isolate was included in the 'decision factor,' in addition to two other factors; the growth rate and pathogen inhibition. By using a statistically designed method, an isolate from the soil was selected and identified as Bacillus sp. GB 16. In the pot test, this strain showed the best performance among the isolated strains. The lowest disease incidence rate and fastest seed growth were observed when the Bacillus sp. GB 16 was used. The action of the surface tension reducing component was assumed to enhance the wetting, spreading, and residing of the antagonistic strain in the rhizosphere. This result showed that the improved selection method was quite effective in selecting the best antagonistic strain for the biological control of soilborne infectious plant pathogens.

• Journal of Biosystems Engineering
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• v.28 no.5
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• pp.395-402
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• 2003

Standard ginseng seedling, selected after one year's rearing in the seedling bed has been conventionally transplanted in Korea. Recently, the direct seeding areas have been increasing to product high quality ginsengs for red-ginseng processing. Reciprocating push-rod brush, holed-roller and vacuum suction seeders, planting with 30 ${\times}$ 30mm spacings and adjustable to the folds were designed and theirs performances were evaluated. The developed three types of seeders reliably performed at the success rate over 95% with less than 10% in slip rate, and could be used a seeder for the greater spacings by adjusting metering parts. The research concluded that the vacuum suction seeder was the most feasible and practical with the greatest metering success and the least slip among three type seeders of the reciprocating push-rod brush, holed-roller and vacuum suction seeders. The vacuum suction seeder holds a seed with each needle nozzle using the negative pressure created by a vacuum pump. The capacity was 24.5 times greater than a man power at the speed of 5 m/min.