• 한국결정학회지
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• 제8권2호
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• pp.75-80
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• 1997

PbMg1/3Mb2/3Oc(PMN)은 대표적인 완화형 강유전체로 완만형상전이 거동을 연구하는데 중요한 재료로 이용되고 있다. 본 연구에서는 양질의 PMN 단결정을 성장시킬수 있는 조건을 결정하고 미세구조를 관찰하고자 하였다. PMN 단결정을 융제법 중 자발핵생성법과 상단종자정법을 이용하여 성장하였다. PbO융제를 이용하여 2-5mm의 결정을 성장시켰고, MgO를 100% 이상 융제로 첨가하였을 때 PMN 결정만이 성장됨을 관찰하였다. Pbo-B2O3 융제를 이용하여 (001)면이 잘 발달한 결정을 얻을 수 있었다. PbO-B2O3 융제를 이용하여 상단종자정법에 의해 1cm이상의 큰 단결정을 성장하였다. 안정된 성장조건을 마련하기 위해서는 종자정의 회전속도와 냉각속도, 종자정의 방향 등의 변수를 최적화하기 위한 계속적인 실험이 필요하다. 박편으로 가공한 결정의 전자선 회절패턴을 통해 미소부위에서 Mg2+와 Nb5+가 1:1의 규칙배역을 하고 있음을 확인하였다.

• 한국약용작물학회지
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• 제28권4호
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• pp.298-309
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• 2020

Background: Safflower (Carthamus tinctorius L.) is a useful medicinal and oil crop in Korea. However, when safflower is cultivated, the flowering period overlaps with the rainy season, and seed maturation is poor. Therefore, this study aimed to use basic research data to develop superior varieties using agronomic characteristics and crossing method. Methods and Results: A total of 34 safflower germplasms were sown and their agronomic characteristics were investigated. Based on these investigations, the cultivar 'ui-san-hong-hwa' was selected as the mother plant, and 'Myanmar safflower' (Hsu Pan) was selected as the father plant. In addition, we developed a floret-protecting cap to cover florets after emasculation during the artificial crossing. When florets were protected by the cap, the seed setting rate increased in comparison to that in the non-covered florets. Conclusions: Agronomic characteristics can contribute to developing suitable varieties. The results suggest that the protection cap will be helpful in breeding without the floral organ drying. This study contributes an efficient breeding method to develop new safflower varieties.

• Journal of Plant Biotechnology
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• 제29권3호
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• pp.185-188
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• 2002

유자의 성숙종자를 MS 기본배지에 치상하여 종자의 내피에서 주심조직 유래의 희고 부서지기 쉬운 배발생캘러스가 1.2%의 빈도로 형성되었다. 이 캘러스는 1 mg/L 2,4-D를 첨가한 MS배지에서 증식되었다. 증식된 캘러스를 0.1 mg/L kinetin을 첨가한 MS 배지에 옮겼을 때 많은 수의 체세포배가 형성되었다. 배발생캘러스를 1 mg/L 2,4-D를 첨가한 액체 배지에 넣어 배발생 현탁배양계를 확립하였다. 배양된 현탁배양세포를 0.5 mg/L ABA를 첨가한 고체배지에 평판하였을 때 높은 빈도로 체세포배로 발달하였으며 MS 기본배지 혹은 1 mg/L kinetin 첨가배지에서 소식물체로 발달하였다. 소식물체는 성공적으로 토양으로 옮겨서 온실에서 육성되었다.

• Nutrition Research and Practice
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• 제13권3호
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• pp.205-213
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• 2019

BACKGROUND/OBJECTIVES: Myocardial infarction (MI) is caused by extensive myocardial damage attributed to the occlusion of coronary arteries. Our previous study in a rat model of ischemia/reperfusion (I/R) demonstrated that administration of arabinoxylan (AX), comprising arabinose and xylose, protects against myocardial injury. In this study, we undertook to investigate whether psyllium seed husk (PSH), a safe dietary fiber containing a high level of AX (> 50%), also imparts protection against myocardial injury in the same rat model. MATERIALS/METHODS: Rats were fed diets supplemented with PSH (1, 10, or 100 mg/kg/d) for 3 d. The rats were then subjected to 30 min ischemia through ligation of the left anterior descending coronary artery, followed by 3 h reperfusion through release of the ligation. The hearts were harvested and cut into four slices. To assess infarct size (IS), an index representing heart damage, the slices were stained with 2,3,5-triphenyltetrazolium chloride (TTC). To elucidate underlying mechanisms, Western blotting was performed for the slices. RESULTS: Supplementation with 10 or 100 mg/kg/d of PSH significantly reduces the IS. PSH supplementation (100 mg/kg/d) tends to reduce caspase-3 generation and increase BCL-2/BAX ratio. PSH supplementation also upregulates the expression of nuclear factor erythroid 2-related factor 2 (NRF2), and its target genes including antioxidant enzymes such as glutathione S-transferase mu 2 (GSTM2) and superoxide dismutase 2 (SOD2). PSH supplementation upregulates some sirtuins ($NAD^+$-dependent deacetylases) including SIRT5 (a mitochondrial sirtuin) and SIRT6 and SIRT7 (nuclear sirtuins). Finally, PSH supplementation upregulates the expression of protein kinase A (PKA), and increases phosphorylated cAMP response element-binding protein (CREB) (pCREB), a target protein of PKA. CONCLUSIONS: The results from this study indicate that PSH consumption reduces myocardial I/R injury in rats by inhibiting the apoptotic cascades through modulation of gene expression of several genes located upstream of apoptosis. Therefore, we believe that PSH can be developed as a functional food that would be beneficial in the prevention of MI.

• 정보보호학회논문지
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• 제16권4호
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• pp.59-68
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• 2006

스마트카드에 내재된 암호 알고리즘이 이론적으로 안전하더라도 실제 구현 환경에 따라 사이드 채널 공격에 취약하다는 사실이 근래에 알려졌다. 본 논문에서는 스마트카드에 구현된 암호 알고리즘의 안전성을 분석할 수 있는 툴을 직접 개발하여 현재 상용 중인 칩을 탑재한 스마트카드에 사이드 채널 공격 중 가장 강력한 공격 방법으로 알려진 전력분석공격과 오류주입공격을 적용하여 안전성 분석을 하였다. 전력분석공격은 대칭키 암호 시스템에 적용하기 쉬운 차분전력분석 공격을 SEED와 ARIA에 대해서 적용하였고, 오류주입공격은 스마트카드의 동작 클럭과 전원을 차단하는 방법으로 CRT기반의 RSA에 적용하였다. 공격 결과 대상 대응책이 없는 경우의 전력분석공격은 가능하지만 오류주입공격은 칩 내부에 사전 방어대책이 마련되어 있어 사이드 채널 공격에 안전했다.

• Journal of Forest and Environmental Science
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• 제37권4호
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• pp.323-330
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• 2021

Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA₃ on the growth of plants germinated from zygotic embryos through GA₃ treatment, the best growth was shown in 0.5 mg/l GA₃ treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.

• 현장농수산연구지
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• 제25권3호
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• pp.35-46
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• 2023

It was in the 1982 that artificial seed production research for olive flounder (Paralichthys olivaceus) farming was first conducted in Korea (Currently, National Institute of Fisheries Science, Fish Breeding Research Center). In 1985, fertilized eggs were obtained from natural olive flounder adapted to land tanks, and artificial seed production technology was established and fertilized eggs were distributed. In the late 1980s, halibut aquaculture began to prosper in land-based tank farming in Jeju Island and Busan's Gijang region, where water temperatures are relatively high in winter. Currently, aquaculture is being carried out all over the country, centering on Jeju Island and Wando, Jeollanam-do. However, olive flounder farming, which started with a small group in the 1980s, reduced genetic diversity through inbreeding over generations, resulting in side effects such as slow growth, reduced resistance to disease and environmental conditions. In order to solve these genetic problems of farmed olive flounder in Korea, the Fish Breeding Research Center of the National Institute of Fisheries Science introduced a wild-caught parent fish group to the existing aquaculture group from 2003 to 2004. Genetic diversity was secured and KingNupchi with fast growth and improved body shape was developed. In this study, the current status of breeding technology development of olive flounder, a major aquaculture breed in Korea, is reviewed and future research directions are suggested.

• Food Quality and Culture
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• 제2권2호
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• pp.73-79
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• 2008

Recently, increasing interests in local foods have been highlighted along with active efforts and activities from autonomy organizations to develop local cultures and promote local economies by generating value added products and profits through the development of local foods. Subsequently, useful effects might result by making local foods attractive to tourists, such as by using indigenous Gimpo products to make menu items that incorporate the social, economic, and cultural aspects of the Gimpo area, and contributing to its food culture, the development and use of its indigenous products, and promoting local restaurant businesses, etc. The items of the survey used to select the local food menu items to be developed were limited to ideas offered by expert groups, and were implemented by a new menu development process. In order to derive the menu items to develop, the following method was applied: a brainstorming session with experts to generate and draft ideas, a questionnaire to chefs and cooks in special grade hotels is Seoul to select the menu items to develop from those drafted, and sensory evaluations by experts to evaluate the developed items. Frequency analysis and technical statistical analysis were performed using the SPSS 12.0 program package, where 13 kinds of local foods were ultimately developed using indigenous products of Gimpo. The developed local food menu items were rice & grape sujaebi, grape sikhae, fried rice embryo bud, grape yanggaeng, rice pancakes, rice spaghetti, grape seed oil dressing, grape sauce, rice pizza, grape pie, rice & grape ice cream, grape chocolate, and rice roll cakes.

• 한국자원식물학회지
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• 제18권2호
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• pp.302-308
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• 2005

본 연구는 금낭화 배우체의 형성과 배 발달특성에 관한 기초 자료로 활용하기 위해 수행되었다. 시원세포로부터 발달된 소포자 모세포는 화뢰의 길이가 1 mm일 때 감수분열을 하여 4면체형(tetrahedral) 4분자가 형성되었다. 4개의 소포자는 분리되어 각각 웅성배우체로 발달하였다. 대포자 모세포는 화뢰의 길이 $4{\sim}5\;mm$에서 관찰되었다. 대포자의 발달유형은 정상형(polygonum)이었으며, 배낭의 형태는 굽어있는 곡생배주(amphitropous)였다. 3개의 뚜렷한 반족세포는 배낭이 성숙한 후에도 퇴화하지 않고 남아있었다. 개화 전 자웅배우체는 충분히 성숙하였다. 개화 시에 수술과 암술의 길이는 거의 비슷하거나 수술이 0.5 mm 짧아서 자화수정에 적당한 구조를 가지는 것으로 나타냈다. 수정 후 배는 구형, 심장형을 거쳐 자엽배까지 발달하였으며, 종자 산포시 초기 자엽배를 가지고 있음을 알 수 있었다.

• 인간식물환경학회지
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• 제24권6호
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• pp.595-608
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• 2021

Background and objective: For effective horticultural education, it is required to develop kits for practice. In this study, we developed a horticultural kit for germination that introduces storytelling, characterization, and assembly, and investigated the educational effects of using it to study its educational differentiation and usefulness. Methods: First, we developed a seed germination kit for learning. It consists of three components: the kit main frame for hydroponics, the activity sheet for storytelling, and the instruction manual. It is made into an acrylic assembly in consideration of the ease of delivery for contactless learning. Second, we developed two programs, each using a germination kit for horticultural education (experimental group) and a general germination kit program (control group). At this time, the class consists of pre-questionnaire, the 1st and 2nd classes, observation log writing (total six times for two weeks), the 3rd class, and post-questionnaire. The experimental group and control group were separately executed. Third, the application results were compared and analyzed before and after class. As a quantification questionnaire, the effects before and after class were compared through t-test with a questionnaire on program satisfaction and attitude change toward plants. The quantitative results were analyzed through observation activity sheets and effects before and after the class were analyzed through t-test with quantification and language coding. Results: The effect of horticulture education using the developed kit was estimated by a quantification questionnaire. Both the program satisfaction and the change in attitude toward plants showed high in the experimental group, and this difference was statistically significant (p < .05). As a result of analyzing the observation paper with a qualitative questionnaire, various cognitive and affective expressions appeared in the experimental group as well as the contents of the observation paper. The number of syllables and various qualitative expressions appeared, and these differences were also statistically significant (p < .05). Conclusion: Compared to general germination activities, it was found that the germination activities using storytelling-based horticultural kits would be effective for contactless classes in the post-COVID-19 era and in increasing the educational effect of horticultural activities.