• Title/Summary/Keyword: detach core

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Process Induced Warpage Simulation for Panel Level Package (기판 소재에 따른 패널 레벨 패키지 공정 단계별 warpage 해석)

  • Moon, Ayoung;Kim, Sungdong
    • Journal of the Microelectronics and Packaging Society
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    • v.25 no.4
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    • pp.41-45
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    • 2018
  • We have simulated the process induced warpage for panel level package using finite element method. Silicon chips of $5{\times}5mm^2$ were redistributed on $122.4{\times}93.6mm^2$ size panel and the total number of redistributed chips was 221. The warpage at each process step, for example, (1) EMC molding, (2) attachment of detach core, (3) heating, (4) removal of a carrier, and (5) cooling was simulated using ANSYS and the effects of detach core and carrier materials on the warpage were investigated. The warpage behaved complexly depending on the materials for the detach core and carrier. However, glass carrier showed the lower warpage than FR4 carrier regardless of detach core material, and the minimum warpage was observed when the glass was used for the detach core and carrier at the same time.

Regulation of SPIN90 by Cell Adhesion and ERK Activation

  • Kim Sung Hyun;Kim Dae Joong;Song Woo Keun
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2004.05a
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    • pp.141-146
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    • 2004
  • SPIN90 was identified to farm molecular complex with $\betaPIX$, WASP and Nck. This complex shows that SPIN90 interacts with Nck in a manner dependent upon cell adhesion to extracellular matrix, but $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex was stable even in suspended cells. This suggests that SPIN90 serves as an adaptor molecule to recruit other proteins to Nck at focal adhesions. SPIN90 was phosphorylated by ERK1, which was, itself, activated by cell adhesion and platelet-derived growth factor. Such phosphorylation of SPIN90 likely promotes the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex and Nck. It thus appears that the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex with Nck is crucial for stable cell adhesion and can be dynamically modulated by SPIN90 phosphorylation that is dependent on cell adhesion and ERX activation. SPIN90 directly binds syndapin I, syndapin isoform II-1 and II-s via its PRD region in vitro, in vivo and also associates with endocytosis core components such as clathrin and dynamin. In neuron and fibroblast, SPIN90 colocalizes with syndapins as puntate form, consistent with a role for SPIN90 in clathrin-mediated endocytosis pathway. Overexpression of SPIN90 N-term inhibits receptor-mediated endocytosis. Interestingly, SPIN90 PRD, binding interface of syndapin, significantly blocks internalization of transferrin, demonstrating SPIN90 involvement in endocytosis in vivo by interacting syndapin. Depletion of endogenous SPIN90 by introducing $\alpha-SPIN90$ also blocks receptor-mediated endocytosis. Actin polymerization could generate farce facilitating the pinch-out event in endocytosis, detach newly formed endocytic vesicle from the plasma membrane or push out them via the cytosol on actin tails. Here we found that SPIN90 localizes to high actin turn over cortical area, actin-membrane interface and membrane ruffle in PDGF treated cells. Overexpression of SPIN90 has an effect on cortical actin rearrangement as filopodia induction and it is mediated by the Arp2/3 complex at cell periphery. Consistent with a role in actin organization, CFP-SPIN90 present in actin comet tail generated by PIP5 $kinase\gamma$ overexpression. Therefore this study suggests that SPIN90 is functional linker between endocytosis and actin cytoskeleton.

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Construction of Environmental Friendly Special-Purpose Ship for the Removal of Blue-green Algae (친환경적 녹조 제거용 특수선박 건조)

  • Shin, Jae-Ki;Yi, Hye-Suk;Jeong, Sun-A;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.42 no.3
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    • pp.404-406
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    • 2009
  • This study note wished to introduce special-purpose ship for algae removal that is developed by core technology of our country. The ship is consisted of main frame and adjuvant that can attach and detach as cross (+) shape of a character. The characteristics of ship are super light weight and low draft. That is consisted of four devices as suction, collection, filtration and recovering units. Among these, filtration used screen filter (mesh size 30 ${\mu}m$). Also, can separate and remove water and algae by compression air participle notion. Percentage of moisture content of concentrated algal particle was 85%. Water parted with algae finally is exhausted to water area. Removal efficiency that compare by chlorophyll-$\alpha$ concentration was about 57% (inflow: 83.2 ${\mu}g\;L^{-1}$, outflow: 35.8 $[\mu}g\;L^{-1}$) without physical and chemical pretreatment. Forward, need to achieve effect test in various conditions (algal biomass, flow etc.) for efficiency and technological elevation of exclusion device. We wished to contribute in presuppression system construction of massive algal development that manage blue-green algae occurrence area effectively, and prevents spread as lower part of reservoir.