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Electron Microscopic Study for the Influence of Soaking in Hot Water and Prochloraz Solution on Spore and Mycelium of Fusarium fujikuroi Infected in Rice Seed (온탕소독과 prochloraz 침지소독이 벼 종자에 감염된 Fusarium fujikuroi의 포자와 균사의 형태에 미치는 영향에 대한 전자현미경적 연구)

  • Park, Woo-Sik;Yeh, Wan-Hae;Lee, Se-Weon;Han, Seong-Suk;Lee, Jun-Seong;Lim, Chun-Keun;Lee, Yong-Hwan
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.176-181
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    • 2008
  • This experiment was conducted to find the causes of ineffective seed disinfection methods such as rice seeds soaking in hot water and prochloraz EC solution when the rice seeds were severely infected by Bakanae disease. In case of rice seeds collected from severely diseased field by Bakanae disease, the pathogen as the forms of spores and mycelium were infected in plumule and inner and outer integument of embryo, aleurone layer, and pericarp layer. When the rice seeds were soaked in hot water, the appearances of spores and hypha on the outer pericarp layer were severely disordered, however those of inner region of outer integument and aleurone layer were shown normal. The membrane of hypha on the outer pericarp layer was destroyed within 24 hours, while some spores were healthy and germinated 7 days after soaking, when the rice seeds soaked 24 hours in 125 ppm prochloraz solution at $30^{\circ}C$. These results indicated that the seed disinfection methods were ineffective on the Bakanae disease severely infected rice seed because the hot water did not transmit the pericarp layer of rice seed and also prochloraz solution did not effectively destroy the spore of pathogen.

Exclusive correlation analysis for algae and environmental factors in weirs of four major rivers in South Korea (4대강 주요지점에서의 조류 발생인자의 배타적 상관성분석에 대한 연구)

  • Lee, Eun Hyung;Kim, Yeonhwa;Kim, Kyunghyun;Kim, Sanghyun
    • Journal of Korea Water Resources Association
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    • v.49 no.2
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    • pp.155-164
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    • 2016
  • Algal blooms not only destroy fish habitats but also diminish biological diversity of ecosystem which results into water quality deterioration of 4 major rivers in South Korea. The relationship between algal bloom and environmental factors had been analyzed through the cross-correlation function between concentration of chlorophyll a and other environmental factors. However, time series of cross-correlations can be affected by the stochastic structure such auto-correlated feature of other controllers. In order to remove external effect in the correlation analysis, the pre-whitening procedure was implemented into the cross correlation analysis. The modeling process is consisted of a series of procedure (e.g., model identification, parameter estimation, and diagnostic checking of selected models). This study provides the exclusive correlation relationship between algae concentration and other environmental factors. The difference between the conventional correlation using raw data and that of pre-whitened series was discussed. The process implemented in this paper is useful not only to identify exclusive environmental variables to model Chl-a concentration but also in further extensive application to configure causality in the environment.

The Comparative Study of Flora around the Geumgang Area (금강 수계의 식물상 비교 연구)

  • Kim, Young-Hyun;Park, Jeong-Mi;Jang, Chang-Gee
    • Korean Journal of Environmental Biology
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    • v.27 no.2
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    • pp.216-229
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    • 2009
  • The flora of vascular plants in the Geumgang area (Mt. Guryongsan, Mt. Jeonwolsan, Mt. Samasan, Mt. Yongmeorisan and Mt. Hamrasan) was investigated to suggest strategies for conservation. This study was performed from May 2007 to April 2008, with total 17 times. The Korean endemic species, legally protected species and species to threaten domestic ecosystem species to threaten were grasped on this study. The flora consists of 334 taxa, 86 families 246 genera 297 species 34 varieies and 3 forms in total results from 5 sites in this study. Korean endemic species were confirmed 8 taxa. No rare and endangered species were found in this study. The 2 taxa of the fourth grade of specially designated plants announced the Ministry of Environment were investigated and 8 taxa of the third grade were confirmed. In totally, 24 taxa that are specially designated plants announced the Ministry of Environment including the first and the second grade taxa. 25 taxa of alien naturalized plants were confirmed. The degree of destroy of forest vegetation and reduction of plant diversity in the midstream of Geumgang were in proceeding by construction of multifunctional administrative city and cultivation. It is needed to set up strategies for conservation forest vegetation in this study area.

Seismic Performance Evaluation of Masonry Walls Retrofitted with Semi-buried Lattice Reinforcement (조적식 구조물의 부분 매입식 격자철근 보강기법의 내진 성능 평가)

  • Kim, Sang Hyo;Choi, Moon Seock;Park, Se Jun;Ahn, Jin Hee
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.15 no.3
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    • pp.88-98
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    • 2011
  • Masonry structure is a style of building which has been widely applied as residential facilities of low and middle stories, commercial and public facilities etc. But it is possible to destroy by loss of adhesive strength or sliding when lateral forces, such as earthquake, occurs. This study proposes a seismic retrofit method for masonry structure and its seismic performance is demonstrated by shaking table test. Two specimens per each shaking direction were made, having out-of-plane(weak axis) and in-plane(strong axis) direction. External load of 1 ton was also applied for each specimen during the test, to model the behavior of reinforced masonry wall. As a result of shaking table tests, it is shown that the specimen applying the proposed seismic retrofit method showed acceptable behaviors in both of Korea building design criteria(0.14g) and USA seismic criteria suggested by IBC(0.4g). However, it was observed that stiffness of the specimen toward out-of-plane was rapidly decreasing when seismic excitations over 0.14g were loaded. In comparison of relative displacements, maximum relative displacement of specimens which were accelerated toward out-of-plane with 0.4g at once was 29~31% of maximum relative displacement when specimens were gradually accelerated from 0.08g to 0.4g, while the maximum relative displacement of specimens accelerated toward in-plane has similar value in both cases. Therefore, it is concluded that the wall accelerated toward out-of-plane is more affected by hair crack or possible fatigues caused by seismic excitation.

Immediate Connection of Customized Zirconia Abutment Using Flapless Guided Surgery: A Clinical Report (무절개 수술을 이용한 맞춤형 지르코니아 지대주의 즉시 장착 증례)

  • Lee, Gyeong-Je;Choi, Byung-Ho;Kim, Hee-Jun;Jung, Seng-Mi
    • Journal of Dental Rehabilitation and Applied Science
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    • v.28 no.2
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    • pp.201-212
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    • 2012
  • There are some similar aspects at histological and morphological characteristics between the peri-implant tissue and periodontal tissue and the direct attachment between the titanium and soft tissue around the implant called as "Functional ankylosis" can prevent the apical infiltration of inflammatory and bone resorption around implant. But, the repeated connection and disconnection of the abutment can destroy the mucosal barrier of soft tissue around the implant and can cause the marginal bone resorption. The amount of marginal bone resorption may reduced if the prosthetic abutment is placed at that time of surgery. Connection of the prosthetic abutment at surgery was limited because the low accuracy of conventional method, but by using of Cone Beam Computed Tomography(CBCT) and guide surgery, the 3-dimensional accuracy of implant placement became much higher than before and it became possible. This is a clinical case of immediate connection of prosthetic abutment and provisional restoration by using of precise CBCT diagnosis and pre-fabricated zirconia customized abutment at surgery and the alternative method is described in this article because of the clinically contentable results.

A literatual study on the Ecthyma (렴瘡에 對한 文獻的 考察)

  • Kim, Hee-Taek;Oh, Young-Seon;Rho, Sek-Seon
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.10 no.1
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    • pp.209-246
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    • 1997
  • In the literatual study on the Ecthyma, the results were as follows. 1. The pathogenic factors of Ecthyma is divided three parts. One is exogenous pathogenic factors which including the wind evil, wind heat and toxic material produced by wetness evil. Another is endogenous pathogenic factors which including the declination of kidney-yang, impairment of the liver and kidney, the lower classes of yin-fire, accumulation of wetness-heat in the spleen asthenia, impairment of the liver and kidney, wetness-heat of three yang, asthenic heat-syndrome of three yin. The other is pathogenic factors neither endogenous nor exogenous which including the food and living, uncontrol sexual excess, anxiety and angry, injury of skin, injury of insects and animals. 2. Five viscera which was concerned with Ecthyma are liver, spleen and kidney. 3. Frequent region of Ecthyma are S-36(足三里) and C-7(陰交). External Ecthyma was rose to wetness-heat of three yang channel that cured easily. Internal Ecthyma was rose to asthenic heat-syndrome of three yin channel that cured hardly. 4. In the frequency of prescription, the most numerous prescription is Bojungikgitang(補中益氣湯) and the next are Kyukgigo(隔紙膏) and Yukmijihwanghwan(六味地滉丸). 5. In the frequency of medicine, the most medicine is Calomelas(輕粉) which included Hydrargyrum(水銀) and the next are Olibanum(乳香) and Resina Commiphorae Myrrhae(沒藥) which regulating vital energy and pain control medicine used that in order to destroy insects and remove polson. 6. In classification of the medical action, medicine of clearing away summer-heat and heat evil and activating blood circulation to dissipate blood stasis used to be very busy which in order to remove the disorder of vital energy for virulent heat-evil. 7. In classification of four characters, the most part is warm medicine, the next are cold and cool medicine and there is a few that is hot medicine. 8. In classification of five tastes, the most numerous tastes are bitter and acrid, the next are sweet, salty and sour tastes. 9. In classification of virulence of medicine, the most part is non-toxic, the next are weakly and deadly poison. 10. In classification of channel distribution, the most is the medicine that belongs to liver channel, the next are the lung, spleen, stomach and kidney channel.

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Solar Detoxification of Trichloroethylene in Waste Water with Slurry Batchtype Photoreactor (Slurry batch형 광화학 반응기를 이용한 폐수 내의 Trichlroethylene의 분해)

  • Lee, Tai-K.;Kim, Dong-H.;Cho, Sug-H.;Auh, Chung-Moo
    • Solar Energy
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    • v.12 no.3
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    • pp.10-20
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    • 1992
  • In this experiment, photochemical reaction has been applied to destroy TCE in water phase. The main target of this work is to investigate the technical feasibility of large scale of solar detoxification reactor for water treatment. The results have revealed that solar detoxification utilizing photon energy from the sun is the most attractive process to decompose organic toxins in water phase at room temperature. The detailed results from this work are as follows; (1) The highest conversion ratio of TCE was obtained by using $TiO_2$, annatase as a photocatalyst among $TiO_2$ anatase, $TiO_2$ rutile and $V_2O_5$ under the same experimental condition. The anatase crystal structure was confirmed with XRD analysis, and its surface area was 7.748 $m^2/g$ from the BET-$N_2$ measurement (2) 0.1 wt% of $TiO_2$ anatase has been adopted as optimal quantity for batch slurry reactor at this experimental conditions. (3) The effect of hydrogen peroxide on the conversion of TCE was investigated. Its optimal quantity was 0.06 vol. % under this experimental conditions. (4) The effect of oxygen on the conversion of TCE also was studied by controlling the head space in photoreactor. Results indicated that sufficient amount of oxygen should be supplied to accomplish the highest conversion rate of TCE in water phase.

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Physicochemical Characteristics and Antioxidant Activities of Freezing Pretreated Black Garlic (동결 전처리한 숙성 흑마늘의 이화학적 특성 및 항산화 활성)

  • Choi, Hye Jung;Lim, Bo Ram;Ha, Sang-Chul;Kwon, Gi-Seok;Kim, Dong Wan;Joo, Woo Hong
    • Journal of Life Science
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    • v.27 no.4
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    • pp.471-475
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    • 2017
  • Freezing pretreatment can destroy the cell membrane of garlic and may improve some food-quality of garlic. Therefore we investigated the effect of freezing pretreatment at $-20^{\circ}C$ and $-70^{\circ}C$ on quality of aged black garlic, compared with traditional processing methods. Our results showed that freezing pretreatment at $-70^{\circ}C$ had the greatest impact on qualities and antioxidant activities of black garlic. Browning degree and pH of black garlic after both the freezing pretreatment and aging process were 3.14 and 3.55, respectively. Furthermore, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and reducing power of aged black garlic can be enhanced by pre-freezing processing. Reducing sugar and 5-hydroxymethyl-2-furaldehyde (5-HMF) contents of freezing pretreated and aged black garlic were increased by 1.69 and 1.14 fold, respectively, compared with the control samples. The results indicated that freezing pretreatment had improved the overall qualities (such as browning degree, pH, reducing sugar) and functional materials of black garlic.

Characteristics of Stormwater Runoff with respect to Pavement Types (도로 포장방법에 따른 비점오염물질 유출특성 비교)

  • Kim, Cheolmin;Choi, Jiyeon;Lee, Jung Min;Cho, Hyejin;Kim, Lee-Hyung
    • Journal of Wetlands Research
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    • v.16 no.3
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    • pp.423-429
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    • 2014
  • Due to high imperviousness rates of the roads, various pollutants originated from vehicle activities and air depositions are accumulated on the road surfaces. The washed-off pollutants can deteriorate the water quality and destroy the aqua-ecosystems with their toxicity. Usually the roads are paved with asphalt and concrete, which can affect on the pollutant concentrations with different frictional forces. Therefore, this research is performed to evaluate the influences of different pavement type on discharged concentrations of pollutant. The results shows the first flush phenomenon was occurred on both pavement types. However, peak concentrations are higher in concrete pavement areas than asphalt pavement because concrete pavement has high contact area with vehicles. The EMCs(Event Mean Concentration) also shows high values in concrete paved roads. As a result of this research, it can be concluded the pavement type is also one of the important affecting factors on pollutant emissions from the roads.

pH-dependence in the inhibitory effects of Zn2+ and Ni2+ on tolaasin-induced hemolytic activity (Zn2+와 Ni2+에 의한 톨라신 용혈활성 저해효과의 pH 의존성)

  • Yun, Yeong-Bae;Choi, Tae-Keun;Kim, Young-Kee
    • Journal of Applied Biological Chemistry
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    • v.61 no.3
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    • pp.213-217
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    • 2018
  • Tolaasin secreted by Pseudomonas tolaasii is a peptide toxin and causes brown blotch disease on the cultivated mushrooms by collapsing cellular and fruiting body structure. Toxicity of tolaasin was evaluated by measuring hemolytic activity because tolaasin molecules form membrane pores on the red blood cells and destroy cell membrane structure. In the previous studies, we found that tolaasin cytotoxicity was suppressed by $Zn^{2+}$ and $Ni^{2+}$. $Ni^{2+}$ inhibited the tolaasin-induced hemolysis in a dose-dependent manner and its $K_i$ value was 1.8 mM. The hemolytic activity was completely inhibited at the concentration higher than 10 mM. The inhibitory effect of $Zn^{2+}$ on tolaasin-induced hemolysis was increased in alkaline pH, while that of $Ni^{2+}$was not much dependent on pH. When the pH of buffer solution was increased from pH 7 to pH 9, the time for 50% hemolysis ($T_{50}$) was increased greatly by $100{\mu}M$ $Zn^{2+}$; however, it was slightly increased by 1 mM $Ni^{2+}$ at all pH values. When the synergistic effect of $Zn^{2+}$ and $Ni^{2+}$ on tolaasin-induced hemolysis was measured, it was not dependent on the pH of buffer solution. Molecular elucidation of the difference in pH-dependence of these two metal ions may contribute to understand the mechanism of tolaasin pore formation and cytotoxicity.