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Electron Microscopic Study for the Influence of Soaking in Hot Water and Prochloraz Solution on Spore and Mycelium of Fusarium fujikuroi Infected in Rice Seed

온탕소독과 prochloraz 침지소독이 벼 종자에 감염된 Fusarium fujikuroi의 포자와 균사의 형태에 미치는 영향에 대한 전자현미경적 연구

  • Park, Woo-Sik (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Yeh, Wan-Hae (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Lee, Se-Weon (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Han, Seong-Suk (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Lee, Jun-Seong (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Lim, Chun-Keun (Division of Bio-Resources Technology, College of Agriculture and Life Science, Kangwon National University) ;
  • Lee, Yong-Hwan (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science)
  • 박우식 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 예완해 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 이세원 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 한성숙 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 이준성 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 임춘근 (강원대학교 농업생명과학대학 응용생물공학과) ;
  • 이용환 (농촌진흥청 국립농업과학원 농업미생물과)
  • Published : 2008.12.01

Abstract

This experiment was conducted to find the causes of ineffective seed disinfection methods such as rice seeds soaking in hot water and prochloraz EC solution when the rice seeds were severely infected by Bakanae disease. In case of rice seeds collected from severely diseased field by Bakanae disease, the pathogen as the forms of spores and mycelium were infected in plumule and inner and outer integument of embryo, aleurone layer, and pericarp layer. When the rice seeds were soaked in hot water, the appearances of spores and hypha on the outer pericarp layer were severely disordered, however those of inner region of outer integument and aleurone layer were shown normal. The membrane of hypha on the outer pericarp layer was destroyed within 24 hours, while some spores were healthy and germinated 7 days after soaking, when the rice seeds soaked 24 hours in 125 ppm prochloraz solution at $30^{\circ}C$. These results indicated that the seed disinfection methods were ineffective on the Bakanae disease severely infected rice seed because the hot water did not transmit the pericarp layer of rice seed and also prochloraz solution did not effectively destroy the spore of pathogen.

본 연구는 키다리병에 심하게 감염된 종자를 온탕침법과 prochloraz 유제로 종자소독 시 그 효과가 떨어지는 원인을 규명하기 위해 시행하였다. 키다리병이 발병한 포장에서 수확한 종자의 경우 병원균이 벼 종자의 배와 배유 안쪽까지 감염되어 있었다. $60^{\circ}C$에서 10분 동안 온탕 소독한 종자의 경우 종피 표면의 균사는 파괴되어 비정상적인 반면 배 주피 안쪽에 존재하는 균사는 정상적인 형태로 존재하였다. 포자는 종피 표면에서 비정상적인 포자와 정상적인 포자를 모두 확인하였다. prochloraz 유제로 $30^{\circ}C$ 24시간 침지소독 한 경우 종피의 균사는 완전히 파괴되었으나 포자는 정상과 비정상 포자 모두 확인할 수 있었다. 이러한 결과를 통해 온탕소독의 경우 종피 같은 물리적 한계로 인해 고열이 병원균에 전달되지 못하고, prochloraz의 경우 병원균 포자를 완전히 사멸시키기 못하기 때문에 종자소독 효과가 떨어짐을 알 수 있었다.

Keywords

References

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