• Macromolecular Research
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• v.15 no.4
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• pp.370-378
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• 2007

Mechanical stimulation is known to activate several cellular signal transduction pathways, leading to the induction of signaling molecules and extracellular matrix (ECM) proteins, thereby modulating cellular activities, such as proliferation and survival. In this study, primary human dermal fibroblasts (HDFs) were seeded onto chitosan-based scaffolds, and then cultured for 3 weeks in a bioreactor under a cyclic strain of 1 Hz frequency. Compared to control samples cultured under static conditions, the application of a cyclic strain stimulated the proliferation of HDFs in I week, and by week 3 the thickness of the cell/scaffold composites increased 1.56 fold. Moreover, immunohistochemical staining of the culture media obtained from the cell/scaffold samples subjected to the cyclic strain, revealed increases in the expression and secretion of ECM proteins, such as fibronectin and collagen. These results suggest that the preconditioning of cell/scaffold composites with a cyclic strain may enhance the proliferation of HDFs, and even facilitate integration of the engineered artificial dermal tissue into the host graft site.

• Korean Journal of Optics and Photonics
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• v.13 no.2
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• pp.155-161
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• 2002

The nail bed is located under the finger nail. The arched portions of the nail bed, which contain a large number of capillary loops, are separated by the valley of the nail bed. The valley of the nail bed does not contain capillary loops. Light is scattered when it propagates through the dermis of skin, and human blood strongly absorbs the light with proper wavelength. By use of the optical properties of the nail bed, we propose an optical technique which extracts the nail bed image of the finger nail. After achieving nail bed images of each individual, we correlated between them. The correlation outputs show that we can identify individuals by comparing the peak heights of the correlation outputs.

• Archives of Plastic Surgery
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• v.32 no.3
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• pp.357-362
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• 2005

The aim of this study is to elucidate the effect of keratinase on epidermis of rat skin. Twenty-five male Sprague-Dolly rats were used. The hair on the back were removed and $2{\times}2cm$ area was marked. The rats were divided five groups; 1) Control group(Co), 2) Cleansing gel group(Cl), 3) Cleansing gel+keratinase group, 4) Exfoliant gel group(Ex), and 5) Exfoliant gel+ keratinase group(Ex+K). The solutions were applied to the back area twice a day for five days. On fifth day, the skins were harvested, fixed and prepared for histologic sections. The thickness of keratin layer, living epidermis, dermis, and cell layer number of living epidermis were measured. In the group containing keratinase(Cl+K, Ex+K), the thickness of keratin layer and living layer were thinner than other groups. However, there were no significant differences of the cell layer number of living epidermis and thickness of the dermis among the five groups. We think the keratinase may have the effect thinning the keratin layer as well as the thickness of living epidermis, without effecting the living cell and dermal component. The keratinase containing soap may be of benefit to remove the excess keratin layers in human.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.3
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• pp.346-352
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• 2008

To investigate goose feather follicle development and difference among the dorsal, ventral, and thoracal tracts during embryonic stage, the present study was conducted on 180 embryos at different ages obtained from the Jilin White goose, a Chinese indigenous breed. The study indicated that the epidermis and dermis of goose embryo formed between embryonic day 10 (E10) and 12 (E12). The thickness of the epidermis remained unchanged until hatching; while the thickness of the dermis increased throughout embryonic development. The primary feather follicles formed around E13-E14 and there were no new primary feather follicles forming after E18. The secondary feather follicles formed coincidently at E18. The density of primary and secondary feather follicles on the ventral and thoracal tracts were significantly higher than those on the dorsal tract (p<0.05). For primary and secondary follicles, the diameter of the feather bulbs and the depth of the feather follicles on the dorsal tract were much greater than those on the thoracal and ventral tracts (p<0.01), respectively; while the difference between the ventral and thoracal tracts was not significant (p>0.05). It is concluded that the Jilin White goose is of a single-follicle group structure, differing from mammals which are of multiple-follicle group structure.

• Korean Journal of Ichthyology
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• v.13 no.1
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• pp.24-27
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• 2001

The barbel structure of the spined loach Iksookimia longicorpa were investigated histologically. Their barbels consist of epidermis, dermis, and a central rod. The epidermis contains mucous cells, terminal buds, granular cells, and epidermal cells. The mucous cells are thin rims of basophilic cytoplasm and are located at the surface of the epidermis. The terminal bud is basophilic and is situated at the distal portion of the epidermis. The dermis consists of loose connective tissue containing blood vessels, pigment cells, and nerve cells. The central region of cartilage is the innermost region and is enclosed within muscle layers.

• Parasites, Hosts and Diseases
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• v.48 no.3
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• pp.259-261
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• 2010

Human Gnathostoma hispidum infection is extremely rare in the world literature and has never been reported in the Republic of Korea. A 74-year-old Korean man who returned from China complained of an erythematous papule on his back and admitted to our hospital. Surgical extraction of the lesion and histopathological examination revealed sections of a nematode larva in the deep dermis. The sectioned larva had 1 nucleus in each intestinal cell and was identified as G. hispidum. The patient recalled having eaten freshwater fish when he lived in China. We designated our patient as an imported G. hispidum case from China.

• Applied Microscopy
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• v.38 no.2
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• pp.135-140
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• 2008

In this study, I investigated on the ultrastructure of scalp skin from full term baby mummy by using transmission and scanning electron microscopy. The baby mummy was found within the uterus of a 16th century (Joseon Dynasty) mummified woman aged 20${\sim}$30 years old. In scanning electron microscopic study, I found that the outer surface of scalp skin containing of sweat gland and stratum corneum are well preserved. The skin of the scalp measured about 1 mm in thickness and the epidermis was well distinguished from the dermis. In transmission electron microscopy, the shape and structure of the epithelial cells were not confirmed. I also observed well preserved collagen fibers composed of collagen fibrils with cross banding pattern ultrastructurally. But, the other connective cells were not observed due to decomposition of the dermis.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.38 no.3
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• pp.158-163
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• 2005

This paper deals with the histology of the barbels of striped sea catfish, Plotosus lineatus (Thunberg). This fish have eight noticeable barbels of two pairs on their maxillary and mandibular. Each barbel is composed of an epidermis, dermis and a central rod of cartilage. The epidermis in the middle part of the maxillary barbel is thicker than those on other parts, and formed of stratified epithelium which contains many cutaneous taste buds and a few small club cells. Number of taste buds increase on the middle and posterior part of each barbel. The dermis consists of loose connective tissue fibers which encloses blood vessels and bundles of nerve fibers. The barbels of this fish can be categorized into stiff and flexible types and are accessory, feeding and sensory structures. Thus we substantiate that they are gustatory receptor organs for this fish.

• Korean Journal of Veterinary Research
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• v.46 no.3
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• pp.275-278
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• 2006

A 7-month-old brown pig with a discoid and pedunculate mass measuring $14.0{\times}12.5{\times}2.5cm$ on the skin of the right shoulder was noted at the slaughter house in Jeju. The surface of mass approximately $7{\times}4cm$ was interfaced with skin. The color of mass with firm consistency was mainly black and partially white on cut surface. Histopathologically, numerous unencapsulated endocrine-like cellular nodules of epithelioid cell type with abundant intracytoplasmic black pigment, melanin, were occupied in dermis and subcutis. Most of nodules in deep dermis were surrounded by lightly pigmented spindle cells and loose fibrous tissues. Mitotic figures were infrequently observed. The overlying epidermis was hyperplastic due to the down-growth of rete peg. Based on the gross and histopathologic findings, this case was diagnosed as cutaneous melanoma. In our best knowledge, this is the first case of swine cutaneous melanoma in Korea.

• Korean Journal of Korean Medical Institute of Dermatology and Aesthetics
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• v.1 no.1
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• pp.16-40
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• 2005

Objective : To study the effectiveness of Yangkyuksanhawtang against Allergic Contact Dermatitis I observed the change of cutaneous shape, histochemistry, immunohistochemistry, and the distribution of apoptotic cells. materials and methods : I divided 4-month-old rats into three groups of 10, which are a contrastive group of having applied Acetone olive oil only, ACD group to have intentionally activated Allergic Contact Dermatitis by DNCB and YST group to give medication of Yangkyuksanhawtang extract. And I observed each group of mice after 24, 48 and 72 hours. results : 1. With the result of Contact hypersensitivity assay, YST group shows appreciably less ear swelling than ACD group. 2. Comparing YST and ACD groups to each other regarding general change of skin, YST group shows less hyperplasia of epidermis, less migration of inflammatory cells and less damage of epidermis than ACD group. 3. Regarding the change of collagen fiber, ACD group has appeared to be low in number of collagen fiber while YST shows similarity with the contrastive group. 4. In dermis YST group has showed lower number of mastocyte than ACD group and is granulated type. 5. In dermis YST group has showed less MAC-1, IL-1 , $IL-2R-\;{\alpha}$ G, ICAM-1 and VCAM-1 than ACD group. 6. The distribution of apoptotic cells has appeared littler in YST group than in ACD 7. Among signal molecule of apoptosis Bcl-2 has distributed more in YST group than ACD group and Bax and Fas has distributed less in YST group than ACD group.