• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.748-755
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• 2014

In the genome annotation of Escherichia coli MG1655, the orf382 (1,149 bp) is designated as a gene encoding an alcohol dehydrogenase that may be Fe-dependent. In this study, the gene was amplified from the genome by PCR and overexpressed in Escherichia coli BL21(DE3). The recombinant $6{\times}$His-tag protein was then purified and characterized. In an enzymatic assay using different hydroxyl-containing substrates (n-butanol, $\small{L}$-threonine, ethanol, isopropanol, glucose, glycerol, $\small{L}$-serine, lactic acid, citric acid, methanol, or $\small{D}$-threonine), the enzyme showed the highest activity on $\small{L}$-threonine. Characterization of the mutant constructed using gene knockout of the orf382 also implied the function of the enzyme in the metabolism of $\small{L}$-threonine into glycine. Considering the presence of tested substrates in living E. coli cel ls and previous literature, we believed that the suitable nomenclature for the enzyme should be an $\small{L}$-threonine dehydrogenase (LTDH). When using $\small{L}$-threonine as the substrate, the enzyme exhibited the best catalytic performance at $39^{\circ}C$ and pH 9.8 with $NAD^+$ as the cofactor. The determination of the Km values towards $\small{L}$-threonine (Km = $11.29{\mu}M$), ethanol ($222.5{\mu}M$), and n-butanol ($8.02{\mu}M$) also confirmed the enzyme as an LTDH. Furthermore, the LTDH was shown to be an ion-containing protein based on inductively coupled plasma-atomic emission spectrometry with an isoelectronic point of pH 5.4. Moreover, a circular dichroism analysis revealed that the metal ion was structurally and enzymatically essential, as its deprivation remarkably changed the ${\alpha}$-helix percentage (from 12.6% to 6.3%).

• 한국콘텐츠학회논문지
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• 제17권11호
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• pp.303-311
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• 2017

국내의 철도작업자의 안전사고는 열차 운행 기관사의 부주의, 선로변 작업자의 감각차단 현상 및 신호수의 실수 등으로 인해 추돌사고가 주요 원인으로 매년 꾸준히 발생하고 있으며 이러한 안전사고를 예방할 수 있는 대책 마련이 시급하다. 따라서 철도 작업 시, 선로변 작업자가 청각과 시각적으로 열차접근을 검지하여 열차 접근으로부터 대피할수 있는열차접근경보시스템을 개발하였다. 이 시스템은 철도 작업장에서 1.5km 이상 전후방 선로에 열차자동겸지장치를 설치하여 자동으로 열차 진입을 검지하도록 하고 작업장에 설치된 실시간 경보장치에 유무선으로 검지 신호를 전달하도록 하며, 검지신호를 수신한 실시간 경보장치는 LED 경보등 및 사이렌 등으로 작업자에게 경보신호를 주는 방식으로 작동된다. 이전의 시스템에 비해 원거리에 있는 열차접근의 검지가 가능하고 주로 무선통신방식을 채택하여 통신배선작업이 불필요하며 충전식 배터리와 태양광 충전장치를 통해 외부 전원 공급이 어려운 현장의 상황에 적합한 이점이 있다. 시스템의 현장검증을 통하여 열차자동검지장치와 실시간 경보장치의 성능평가를 실시하였고 100%구동을 확인하여 신뢰성을 검증하였다.

• 만화애니메이션 연구
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• 통권40호
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• pp.315-336
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• 2015

그 시대의 대중에게 설득력을 갖기 위해서는 허구의 세계 역시 관객과 공유할 수 있는 가치관을 가져야하므로, 여러 번 영화화된 이야기의 주인공 캐릭터들은 당대 관객의 최대공감을 얻기 위해 시대에 따라 조금씩 변화를 거친다. 본 논문은 다양한 신데렐라 동화와 영화에 등장하는 주요 캐릭터들이 시대에 따라 어떻게 변천되어 왔는가를 살펴보았다. 20세기 중반까지 제작된 신데렐라 영화의 주인공은 페로 버전의 동화 원전이 그랬듯 유약하며 소극적이고 순종적 성격으로 묘사되다가 70년대가 넘어가면서 자신의 감정과 의도를 가지고 움직이는 신데렐라가 등장하기 시작한다. 1990년대 이후에는 자신에게 닥친 상황을 책임질 수 있도록 강인하게 자신의 능력을 키우는 주인공을 볼 수 있으며 최근에는 부족한대로의 자기 자신을 받아들이고 운명을 스스로 개척해나가는 주인공을 만나게 된다. 왕자 캐릭터 역시 비슷한 변천을 겪는다. 비교적 초기에 만들어진 신데렐라 버전들에서 왕자는 그저 왕자라는 이유로 모든 여성이 선망하고 있다는 것이 당연시 될 뿐 거의 실체가 없는 막연한 존재다. 70년대 이후 만들어진 영화들에서는 나름의 고민과 감정을 가진 왕자가 묘사되기 시작하며 최근에는 점차 철부지 도련님에서 자신의 의무와 책임을 아는 성인으로 성장해가는 모습이 영화에 담기고 있다. 현대 사회에 들어와 상대적으로 여성들에게 사회적 기회가 늘어나면서 더 이상 여성의 신분상승이 남성과의 관계에서만 얻어지지는 않는다. 그에 따라 신데렐라 이야기는 과거만큼의 인기를 누리고 있지는 않으며, 누군가가 신데렐라를 꿈꾼다고 하면 사회적 질타의 대상이 되기 쉬운 것이 사실이다. 그러나 성의 역할을 떠나 신데렐라는 현 상태에서 자신에게 성공의 가능성이 없다는 무력감을 느끼는 사람이 꿈꿀 수 있는 희망이고, 따라서 그러한 사회적 박탈감을 느끼는 사람들이 있는 한 신데렐라 이야기의 변주는 계속될 것이다.

• 한국수산과학회지
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• 제30권1호
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• pp.46-54
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• 1997

분열효모 S. pombe의 포자형성은 배지상의 질소원의 고갈에 의하여 유도되어진다. 감수분열로부터 포자형성에 도달하는 과정에는 다수의 특이적인 유전자가 기능을 하고 있다. 본 연구에서는, 전포자막 구축에 필수적인 유전자 spo 5의 발현조절과 유전자의 메커니즘에 관하여 조사하였다. spo 5 유전자를 보유하는 약 5kb의 Hind III DNA 단편을 cloning 하였다. 이 단편으로부터 제한효소지도를 작성하여 얻어진 DNA 단편을 probe로 하여, RNA blot-hybridization를 이행하였다. 이 결과, 최소배지의 hetro matting-type 균주 (CD16-1)로 부터 조제한 mRNA가 검출되었다. 그리고 이 전사산물을 전사레밸에서 해석하기 위하여, homo matting-type (CD16-3) 균주를 질소원이 함유되지 않은 포자형성배지에서 배양한 후, 동일한 방법으로 mRNA를 조제하여 Northern hybridization으로 조사하였다. 그 결과, 이들 세포에서는 3.2kb에서만 전사산물이 검출되었으며, 2.5kb의 mRNA는 검출되지 않았다. 이상의 결과로 부터 spo 5 유전자를 coding하는 전사산물인 2.5kb의 mRNA는 질소원의 고갈된 상태하에서, 접합형 유전자좌의 hetro 접합성을 요구하는 것으로 입증하였다. spo 5 유전자의 전사발현은 질소원이 결핍과 접합형 유전자좌의 구성에 따른 환경요인과 유전적 요인에 의해서 제어되어지고 있다는 것을 입증하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제17권4호
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• pp.275-281
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• 2013

Astrocytes are reported to have critical functions in ischemic brain injury including protective effects against ischemia-induced neuronal dysfunction. Na-K ATPase maintains ionic gradients in astrocytes and is suggested as an indicator of ischemic injury in glial cells. Here, we examined the role of the Na-K ATPase in the pathologic process of ischemic injury of primary cultured astrocytes. Chemical ischemia was induced by sodium azide and glucose deprivation. Lactate dehydrogenase assays showed that the cytotoxic effect of chemical ischemia on astrocytes began to appear at 2 h of ischemia. The expression of Na-K ATPase ${\alpha}1$ subunit protein was increased at 2 h of chemical ischemia and was decreased at 6 h of ischemia, whereas the expression of ${\alpha}1$ subunit mRNA was not changed by chemical ischemia. Na-K ATPase activity was time-dependently decreased at 1, 3, and 6 h of chemical ischemia, whereas the enzyme activity was temporarily recovered to the control value at 2 h of chemical ischemia. Cytotoxicity at 2 h of chemical ischemia was significantly blocked by reoxygenation for 24 h following ischemia. Reoxygenation following chemical ischemia for 1 h significantly increased the activity of the Na-K ATPase, while reoxygenation following ischemia for 2 h slightly decreased the enzyme activity. These results suggest that the critical time for ischemia-induced cytotoxicity of astrocytes might be 2 h after the initiation of ischemic insult and that the increase in the expression and activity of the Na-K ATPase might play a protective role during ischemic injury of astrocytes.

• 한국콘텐츠학회논문지
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• 제18권10호
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• pp.217-228
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• 2018

본 연구의 목적은 귀농귀촌에 대한 원주민의 인식과 욕구를 살펴보고, 그 결과를 기초로 원주민의 입장이 고려된 귀농귀촌정책 사업들을 제언하는 데에 있다. 본 연구는 귀농귀촌에대한 원주민의 경험을 심층적으로 파악함으로써 귀농귀촌에 대한 원주민의 인식과 욕구를 면밀히 알아보기 위해 초점집단인터뷰 방법을 활용하였다. 연구결과, 원주민이 느끼는 '상대적 박탈감', '귀농귀촌의한계와 어려움', '성공적인 정착을 위한 방안'과 같이 총 3개의 상위범주로 분류되었다. 본 연구의 결과를 통한 구체적인 함의는 첫째, 원주민이 인식하는 귀농귀촌은 정부정책의 불만과 귀농귀촌인의 태도가 귀농귀촌에 대한 부정적 인식을 조성하는 것으로 나타났다. 둘째, 귀농귀촌의 한계와 어려움으로서 경제적 어려움과 안정적인 농작물 경작 및 농사기술 습득의 어려움 등이 나타났다. 셋째, 원주민이 생각하는 귀농귀촌인과의 관계형성 및 소통과 화합에 대한 인식으로는 원활한 관계형성을 위해 귀농귀촌인들과 직접적인 소통을 하고 정보를 교환할 수 있는 모임의 활성화와 소통과 협업을 위해 귀농귀촌인들이 농촌문화를 이해하고 배우려는 자세와 노력이 필요하다는 것으로 나타났다. 넷째, 귀농귀촌과관련된 정보제공 및 지원을 위한 구심점 역할을 하는 기관이 부족함을 알 수 있다. 다섯째, 원주민이 생각하는 귀농귀촌인의정착을 위한 방안들이 도출되었다. 여섯째, 귀농귀촌인의정착과 원주민과의 관계증진이 함께 이루어질 수 있게끔 원주민들이 귀농귀촌인의 멘토가 되는 것이 필요한 것으로 보인다.

• The Korean Journal of Physiology
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• 제10권2호
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• pp.39-45
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• 1976

This study was conducted to see whether the hippocampectomy exerted facilitatory influence upon gastric ulceration in animals, and if so, whether the effect of hippocampectomy could be suppressed by adrenalectomy. 107 male rats were divided into 5 groups: rats that had over 90% of their hippocampal tissue removed through an opening on each side of the cerebral cortex(hippocampal group, N=21), rats that received bilateral adrenalectomy(adrenal group, N=29), rats that received adrenalectomy as well as hippocampectomy(hippocampo-adrenal group, N=10), rats that received damage to each side of the cortex over the hippocampus(cortical control group, N=20), and rats that had solely their head skin incised(normal control group, N=27). All rats were kept without restraint or food deprivation until on the 25th day after surgery, the stomach of each rat was inflated with 7ml of physiological saline and then removed under deep anesthesia. The mucosal surface was sketched under dissecting microscope, and enlarged photographs$(4{\times})$ were taken. The percentage of animals developing gastric ulcer in each animal group was calculated, the number of ulcer in each stomach was counted, and the total area of ulceration per stomach was measured on the Photograph with the aid of superimposed graph paper and expressed as permillage of total area of the glandular mucosa. Results obtained were as follows: 1. The percentage of animals developing gastric ulcer was significantly larger in the hippocampal group than they were in the hippocampo-adrenal, the adrenal, the cortical, and the normal control groups. 2. The mean number of ulcer per stomach was significantly larger in the hippocampal group than they were in the adrenal, the cortical control, and the normal control groups, while no significant difference existed between the hippocampal and the hippocampo-adrenal groups. 3. Total area of ulcer per stomach was significantly larger in the hippocampal group than they were in the cortical control and the normal control groups, but no significant differ-ence existed among the hippocampal, the adrenal, and the hippocampo·adrenal groups. 4. All measured values of the adrenal group were not significantly different from those of the hippocampo-adrenal, the cortical control, and the normal control groups. It is inferred from the above results that the hippocampus exerts an inhibitory influence upon gastric ulceration and that the hippocampal influence is mediated only partly through suppression of pituitary·adrenal activity.

• 한국동물위생학회지
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• 제24권4호
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• pp.379-382
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• 2001

To confirm the effect of food and water deprivation prior to Newcastle disease(ND) virus vaccination, three hundred chicks were divided into five groups with three replications. ND vaccine were sprayed to at 1 -day old chicks at commercial hatchery. Secondary and third vaccination was conducted at 2-week old and 24-day old chicks by LaSota strain. Control was conventionally vaccinated without withdrawing the food and water before or after vaccination. In group 2(G2) and 3(G3), LaSota strain was vaccinated to chicks before and after fasting the food and water for 3 and 2 hours, respectively. Group 4(G4) has the same fasting time of group 2, but supplemented the skim milk in vaccin dilution water. In group 5(G5), skim milk was added into group 3. Weight gain, feed intake and feed conversion were weekly measured for 5 weeks. Blood was collected from wing vein at 24 and 35 days of age. Each serum antibody level were measured by hemagglutination inhibition(HI) test. The average weight gain, feed intake, feed conversion of all group were not significantly different. Weight gain of each groups was 1910.30(control), 1875.28(G2), 1952.12(G4) and 1896.05(G5), respectively. Feed intake of all group was recorded at 3160.67(control), 3167.07(G2), 3189.48(G3), 3157.85(G4) and 3178.16(G5), respectively. The feed conversion of each groups was 1.655(control), 1.688(G2), 1.633(G3), 1.699(G4) and 1.676(G5), respectively. The HI titer of G4 was $ 5.50{\Pm}$1.40 and significantly higher than the other groups (p<0.05)(control : $4.36{\Pm}$1.87 , G2 : $5.18{\Pm}$2.14, G3 : $4.51{\Pm}$2.19, G : $5.28{\Pm}$1.58 at 35 days old. The results of this experiment indicated that two or three hours of fasting time before or after vaccination would be able to show the higher antibody level against ND virus.

• Radiation Oncology Journal
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• 제32권3호
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• pp.187-197
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• 2014

Purpose: We aimed to analyze the treatment outcome and long-term toxicity of 70 Gy hypofractionated intensity-modulated radiotherapy (IMRT) for localized prostate cancer using a customized rectal balloon. Materials and Methods: We reviewed medical records of 86 prostate cancer patients who received curative radiotherapy between January 2004 and December 2011 at our institution. Patients were designated as low (12.8%), intermediate (20.9%), or high risk (66.3%). Thirty patients received a total dose of 70 Gy in 28 fractions over 5 weeks via IMRT (the Hypo-IMRT group); 56 received 70.2 Gy in 39 fractions over 7 weeks via 3-dimensional conformal radiotherapy (the CF-3DRT group, which served as a reference for comparison). A customized rectal balloon was placed in Hypo-IMRT group throughout the entire radiotherapy course. Androgen deprivation therapy was administered to 47 patients (Hypo-IMRT group, 17; CF-3DRT group, 30). Late genitourinary (GU) and gastrointestinal (GI) toxicity were evaluated according to the Radiation Therapy Oncology Group criteria. Results: The median follow-up period was 74.4 months (range, 18.8 to 125.9 months). The 5-year actuarial biochemical relapse-free survival rates for low-, intermediate-, and high-risk patients were 100%, 100%, and 88.5%, respectively, for the Hypo-IMRT group and 80%, 77.8%, and 63.6%, respectively, for the CF-3DRT group (p < 0.046). No patient presented with acute or late GU toxicity ${\geq}$grade 3. Late grade 3 GI toxicity occurred in 2 patients (3.6%) in the CF-3DRT group and 1 patient (3.3%) in the Hypo-IMRT group. Conclusion: Hypo-IMRT with a customized rectal balloon resulted in excellent biochemical control rates with minimal toxicity in localized prostate cancer patients.

• Radiation Oncology Journal
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• 제31권4호
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• pp.199-205
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• 2013

Purpose: To assess the clinical efficacy and toxicity of whole pelvic intensity-modulated radiotherapy (WP-IMRT) for high-risk prostate cancer. Materials and Methods: Patients with high-risk prostate cancer treated between 2008 and 2013 were reviewed. The study included patients who had undergone WP-IMRT with image guidance using electronic portal imaging devices and/or cone-beam computed tomography. The endorectal balloon was used in 93% of patients. Patients received either 46 Gy to the whole pelvis plus a boost of up to 76 Gy to the prostate in 2 Gy daily fractions, or 44 Gy to the whole pelvis plus a boost of up to 72.6 Gy to the prostate in 2.2 Gy fractions. Results: The study cohort included 70 patients, of whom 55 (78%) had a Gleason score of 8 to 10 and 50 (71%) had a prostate-specific antigen level > 20 ng/mL. The androgen deprivation therapy was combined in 62 patients. The biochemical failure-free survival rate was 86.7% at 2 years. Acute any grade gastrointestinal (GI) and genitourinary (GU) toxicity rates were 47% and 73%, respectively. The actuarial rate of late grade 2 or worse toxicity at 2 years was 12.9% for GI, and 5.7% for GU with no late grade 4 toxicity. Conclusion: WP-IMRT was well tolerated with no severe acute or late toxicities, resulting in at least similar biochemical control to that of the historic control group with a small field. The long-term efficacy and toxicity will be assessed in the future, and a prospective randomized trial is needed to verify these findings.