• Title/Summary/Keyword: density of media

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재조합 Escherichia coli 시스템을 이용한 폐흡충 cystein proteinase의 생산 연구

  • Hong, Seong-Hui;Lee, Gil-Hwan;Jeon, Hui-Jin;Hwang, Hyeon-A;Park, Seong-Ryeol;Hwang, Yeong-Bo;Park, Hyeon
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.651-654
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    • 2000
  • A fermentation study of the recombinant Escherichia coli system has been tired to overproduce a recombinant Paragonimus westermani cysteine proteinase, rPwCP1. Using the modified LB media, main cultures and chemical induction with IPTG were carried out to examine the possibility of secretory protein overproduction at high cell density culture. As a result, the target protein of rPwCP1, purified by metal affinity chromatography and gel filtration, has been shown at 50.8 kDa on SDS-PAGE, and its final concentration turns out to be 350mg/L.

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Axiomatic Approach for Design Appraisement and Development DVD (I) (DVD 설계평가 및 개선을 위한 공리적 접근 (I))

  • Moon, Yong-Rak;Cha, Sung-Woon;Kim, Young-Kyu
    • Journal of the Korean Society for Precision Engineering
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    • v.16 no.5 s.98
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    • pp.124-131
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    • 1999
  • DVD(Digital Versatile Disk) is the data storage media devised to make high storage density and high speed data input/output possible. For example, a whole length movie can be stored in a disk as large as a CD(Compact Disk). Therefore higher rotational speed and better accuracy in optical pick-up are required compared with the existing optical storage device. Without these operational functional requirements being satisfied DVD will lose its place as the high capacity storage device. This paper evaluated whether the currently developed DVD satisfies the requirements and provides improved methods with the Axiomatic Approach which is superb in evaluating and designing newly concepted designs.

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차세대 고정 및 이동 무선랜의 기술 동향

  • 민승욱;최은영;류득수;이석규
    • Information and Communications Magazine
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    • v.22 no.9
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    • pp.175-186
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    • 2005
  • 향후 수년 내에 흠, 오피스 및 핫스팟 영역에서 초고속 멀티미디어의 서비스 수요가 폭발적으로 증가할 것으로 전망됨에 따라 IEEE 802.11에서는 200-500Mbps급의 차세대 초고속 무선랜 표준으로서 IEEE 802.11n의 표준안을 제정하는 작업을 진행하고 있다. 새로운 표준에는 무선통신의 다양한 기술들이 표준으로 채택될 전망이다. 논의되고 있는 기술들로는 물리계층에서의 다중 안테나의 사용, 송신 빔 형성, 듀얼 밴드 그리고 LDPC (Low Density Parity Check) 등의 기술과 MAC (Media Access Control) 계층에서의 집합 전송, 블록 전송, 링크 적응 기법 등의 채택이 논의되고 있다. 기존의 무선랜 시스템에서 널리 사용되어온 IEEE 802.11a/b/g와의 호환성을 보장한다. 또한 차세대 무선랜은 차량용 통신 장치를 위한 고속 이동성을 보장하는 표준인 IEEE 802.11p에 대한 논의도 함께 이루어지고 있다. 본 고에서는 표준회의에서 논의되고 있는 후보 기술들과 차세대 무선랜의 기술동향을 살펴본다.

Estimation of the Chitinolytic and Antifungal Activity of Streptomyces sp. CA-23 and AA-65 isolates Isolated from Waste Mushroom Media (버섯 폐배지로부터 분리한 방선균 균주 CA-23과 AA-65균주의 키틴 분해능력과 항균력 검정)

  • Shim, Chang-Ki;Kim, Min-Jeong;Kim, Yong-Ki;Jee, Hyeong-Jin;Hong, Sung-Jun;Park, Jong-Ho;Han, Eun-Jung;Kim, Seuk-Chul
    • The Korean Journal of Pesticide Science
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    • v.19 no.4
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    • pp.402-410
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    • 2015
  • The purpose of this study was to estimate the chitinolytic and antifungal activity of Actinomycetes sp.isolated from waste mushroom media. In five kinds of waste mushroom media, Sinyeong mushroom and Yangsongi were the order of the population density of actinomycetes. Totally 91 chitinolytic isolates of Actinomycetes sp. were obtained from waste mushroom media. The isolates were categorized into 3 groups based on chitinolytic activity and antagonisms against Phytophthora capsici, Rhizoctonia solani, Sclerotinia sclerotiorum, Collectotrichum gloeosporioides, and Cladosporium cucumerinum in vitro. CA-23 was selected as a representative isolate of a group showing strong chitinolytic and antagonistic activities to all of the plant pathogens, while AA-65 was selected as a representative isolate showing no chitinolytic activities but strong antagonistic activities to the pathogens. CA-23 and AA-65 were highly effective on control of Phytophthora blight of hot-pepper, powdery mildew and scab of cucumber in a greenhouse tests. Among the isolates tested, CA-23 showed highest control efficacy, while AA-65 not only effectively controlled the diseases but also consistently increased plant growth and yield. Although the isolates are similarly affected on suppression of plant pathogens, the isolates could be differ from each other in modes of action. Further studies on mechanisms and practical applications are being progressed.

The Characteristics of Manufacture Filter Media for Water Treatment Using Mixture Response with Ash and Food Waste (연소재 및 식품폐기물의 혼합 반응에 따른 수처리 여과재 제조 특성)

  • Park, Seung-Do;Lee, Won-Ho;Lee, Min-Hee
    • Journal of the Korean GEO-environmental Society
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    • v.19 no.5
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    • pp.5-12
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    • 2018
  • The porosity formation by the addition of additives was found to be the highest in the case of aluminum powder 3% and $Ca(OH)_2$ 2% under the condition that strength was maintained. The optimum mixing ratio of the binder was shown to be the most effective at (Ash+Food waste+clay):(water glass+colloidal silica) 7:3, and the temperature response is most economical and effective at $1,000^{\circ}C$. The optimal mixing ratio is the strength in 30% of ash, 30% of clay and 10% of food waste, which is the effective in non-point pollution water treatment. Filter media produced under optimal mixing conditions were analyzed as $SiO_2$ 65.8%, density $1.4g/cm^3$, porosity 25.6%, pH 9.8, and no hazardous substances were detected. As a result of the filtration of the water treatment, the mean concentration of the filtered SS was $14.06mg/{\ell}$, and the removal efficiency of SS was 90%, the recovery rate of the reversal is 97.1%. This enables the development of filter media considering economic efficiency and efficiency as well as the utilization of waste resources, enabling high value added of waste resources.

Feasibility Evaluation for Remediation of Groundwater Contaminated with Heavy Metal using Calcium Polysulfide in Homogeneous media (균질한 매질 내 Calcium polysulfide 주입에 따른 고농도 중금속 오염 지하수 정화 타당성 검토)

  • Hyeon Woo Go;Jin Chul Joo;Kyoungphile Nam;Hee Sun Moon;Sung Hee Yoon;Dong Hwi Lee;So Ye Jang
    • Journal of Soil and Groundwater Environment
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    • v.28 no.1
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    • pp.1-14
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    • 2023
  • In this study, column tests using relatively uniform Jumunjin sand media were conducted to evaluate the feasibility of calcium polysulfide (CaSx, CPS) in removing high concentration of Zn2+ in groundwater. The injected CPS solution reacted rapidly with Zn2+ in artificial groundwater and effectively reduced Zn2+ by more than 99% through metal sulfide precipitation. Since the density (d = 1.27 g/cm3 ) of CPS solution was greater than that of water, CPS solution settled down rapidly while capturing Zn2+ and formed stable CPS layer similar to dense nonaqueous phase liquid. Mass balance analysis on Zn2+ in CPS solution suggested that CPS solution effectively reacted with Zn2+ to form metal sulfide precipitates except for high groundwater seepage velocity of 400 cm/d. With greater groundwater seepage velocity, injected CPS did not completely dissolve at the CPS-water interface, but a partially-misible CPS layer continuously moved and reacted with Zn2++ in the direction of groundwater flow. Since hydraulic conductivity (Kh) decreased slightly due to the generated metal precipitates in the inter-pores of media, injection of CPS solution should be optimized to prevent clogging. As evidenced by both XRF and SEM/EDS results, ZnS precipitates were clearly observed through the reaction between the CPS solution and Zn2+. Further study is warranted to evaluate the feasibility of CPS to remove high-concentration heavy metalcontaminated groundwater in complex and heterogeneous media.

Soil Media and Seeding Rates for the Establishment of Kentucky bluegrass Carpet-type Sod over a Plastic Sheet (플라스틱 시트 위에 재배한 켄터키 블루그래스(Poa pratensis) 카펫형 뗏장의 배양토 및 파종량)

  • Shim, Sang-Ryul;Jeong, Dae-Young
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.2 no.1
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    • pp.20-28
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    • 1999
  • Nine soil media when placed over a plastic sheet with three seeding rates were evaluated for influence on covering rate, height, growth, surface hardness, tear strength and sod establishment of Kentucky bluegrass. 1. Bark, peat and vermiculite over a plastic sheet had good effect in terms of the establishment of Kentucky bluegrass sod. 2. The good establishment of Kentucky bluegrass sod grown on bark, peat and vermiculite over a plastic sheet seemed to be caused by physical and chemical properties of each soil medium. 3. Sand, sandy loam, perlite and peatmoss caused poor effects on the covering rate, the growth, and the tear strength of Kentucky bluegrass. 4. Optimum seeding rate was $10g/m^2$ in terms of density and competition. 5. Good quality sod more depended on soil media than on seeding rates in this study.

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Development of Hydroponic Media Using Fly Ash and Clay System Cultures (양액재배용 석탄회-점토계 배지 개발)

  • 김일섭;강위수;신대용;류근창
    • Journal of Bio-Environment Control
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    • v.9 no.1
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    • pp.47-59
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    • 2000
  • In order to investigate the physical and chemical properties of artificial culture media, the specimens were substituted with 5~20% clay, 10~30%(w) quick lime, 5~l5%(w) burnt plaster and 10%(w) sawdust. Fly ash-clay bodies were sintered at 1,050~1,20$0^{\circ}C$ and then their properties were determined. It was found that 90FA10JC(fly ash +clay(90:10, %(w)) specimen sintered at 1,15$0^{\circ}C$ for 10 min. had good physical and chemical properties. When this composition was supplement with 10%(w) sawdust, bulk density water absorption, apparent porosity, compressive strength and pH after 240 hrs curing time were 1.14, 54.4%, 39.5%, 54 kgf.cm$^{-2}$ and 7.1 respectively. The physical properties of fly ash-quick lime-burnt plaster system specimens were superior to FAJC systems. However, this composition we not suitable as a artificial culture media because of its high pH. In this study, it was shown that 90FA10JC10SD(90FA10JC +10%(w) sawdust) system exhibited the best physical properties.

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Effect of Seeding Rates on Turf Vegetation of Creeping Bentgrass (파종량이 Creeping Bentgrass 잔디초지의 식생에 미치는 영향)

  • Cho, Nam-Ki;Kang, Young-Kil;Song, Chang-Khil;Cho, Young-Il;Park, Sung-Jun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.2
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    • pp.131-136
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    • 2005
  • This study was conducted from March 21 to July 7 in 2004 at JeJu Island to investigate the influences of seed-ing rates (4, 6, 5, 10 and 12 kg/10a) on creeping bentgrass vegetation. The result obtained were summarized as follows; plant height was getting longer as seeding rate increased from 4 to 12 kg/10a, Although it was no significance from 10 to 12 kg/10a. Root length, Minolta SPAD-502 chlorophyll reading value, leave and root weight increased as the plant height increased. The degree of land cover and density of creeping bentgrass also increased as seeding rate increased from 4 to 12 kg/10a, and the degree of land cover and density of weed decreased. The number of weed species on decreased as increasing of seeding rate. Then ranking of the dominant weeds were Digitaria adscendens, Chenopodium album var. centrorubrum md Poa annua (at 4 kg/10a seeding rate), Digitaria adscendens, Chenopodium album var. centrorubrum and Stellaria media (at 6 kg/10a seeding rate). Chenopdium album var. centrorubrum, Poa annua and Digitaria adscendens (at 8 kg/10a seeding rate), Digitaria adscendens, Chenopdium album var. centrorubrum and Steilaria media (at 10 kg/10a seeding rate), Chenopdium album var. centrorubrum, Digitaria adscendens and Stellaria media (at 12 kg/10a seeding rate). These results showed that the optimum seed-ing rate is 10 kg/10a for growth of creeping bentgrass in volcanic ash soils of Juju island.

Changes in Magnetic Properties When Manufacturing Cobalt-substituted Barium Ferrite Powder (Cobalt가 치환된 Barium Ferrite 분말 제조 시 자기적 특성변화)

  • Um, Myeong-Heon;Yeon, Je-Uk;Lee, Cha-Jin;Ha, Beom-Yong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.10
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    • pp.30-39
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    • 2020
  • Single-phase barium ferrite powder was synthesized using the sol-gel method. At this time, an attempt was made to find the optimal experimental conditions for the production of single-phase barium ferrite by varying the Fe to Ba molar ratio (Fe/Ba) and the heat treatment temperature. In addition, cobalt-substituted barium ferrite particles were prepared using cobalt, which has an excellent effect on coercivity control for the production of ferrite fine particles having a coercivity of 2.5 to 5.5 kOe for use in high-density magnetic recording media. The changes in the magnetic properties of these were investigated. X-ray diffraction (XRD), thermogravimetric-differential thermal analysis (TG-DTA), and field emission scanning electron microscopy (FE-SEM) were used to observe the synthesis of single-phase, and Fourier transform infrared spectroscopy (FT-IR) and energy dispersive X-ray spectrometry (EDS) were used to analyze the chemical structure and composition. The coercivity of the cobalt-substituted barium ferrite powder was measured by vibrating sample magnetometry (VSM). As a result, single-phase Barium ferrites were synthesized when the Fe/Ba molar ratio was 10, and the heat treatment temperature was 900 ℃. The coercivity decreased with increasing the amount of Co added. Barium ferrite, having a coercivity of 2.5 to 5.5 kOe for use in high-density magnetic recording media, was synthesized when the Co to Fe(Co/Fe) molar ratio was less than 0.16.