• Title/Summary/Keyword: degree of contamination

Search Result 337, Processing Time 0.023 seconds

Application of ATP Bioluminescence Assay for Measurement of Microbial Contamination in Fresh-cut Produce Processing Lines (신선편이 농식품 생산라인의 환경미생물 오염도 측정을 위한 ATP 검사법의 이용)

  • Choi, Ji-Weon;Lee, Hye-Eun;Kim, Chang-Kug;Kim, Won-Bae;Kim, Ji-Kang
    • Food Science and Preservation
    • /
    • v.19 no.1
    • /
    • pp.62-66
    • /
    • 2012
  • With the rapid growth of fresh-cut produce market, the South Korean fresh-cut industry is facing the challenge of ensuring food safety. As the estimation of the microbial numbers in fresh-cut produce processing lines (tools, and equipment) using the conventional microbiological techniques takes days, so there is a need for faster and easier monitoring methods. This study was conducted to investigate the use of ATP bioluminescence assay to measure the degree of microbial contamination from three actual fresh-cut processing lines. The samples collected from frech-cut vegetables, and fresh-cut fruits processing plants were tested for the estimation of the bacterial number, using the ATP bioluminescence and microbiological methods. The result of former was transferred to log RLU/100 $cm^2$, and that of the latter was transferred to log CFU/100 $cm^2$. A positive linear correlation between the ATP bioluminescence assay value and aerobic-plate count was found for fresh-cut processing lines, with a correlation coefficient of 0.8772 (n=50). The results of this study indicate that ATP bioluminescence assay can be used to monitor microbial contamination in fresh-cut produce processing plants, and can help improve the hygiene therein.

Bacterial Contamination in Disposable Wet Wipes in General Restaurants 2nd (대중음식점 일회용 물티슈의 세균 오염도 조사(2))

  • Chong, Moo-Sang
    • Korean Journal of Clinical Laboratory Science
    • /
    • v.54 no.1
    • /
    • pp.23-27
    • /
    • 2022
  • Disposable wet wipes provided by general restaurants in the Jeju Special Self-Governing Province were evaluated for compliance with the labeling requirements and the degree of bacterial contamination set by the Ministry of Food and Drug Safety. Of the 32 types of disposable wipes, five types of disposable wipes did not indicate the name and location of the business office of the manufacturer. Only 12 types of wet wipes were marked with the date of manufacture and expiration date. Bacterial contamination was seen in 9 types of wet wipes through bacterial culture but these were absent in the remaining 23 types (71.9%). The results of the antimicrobial susceptibility test for the 9 identified strains showed no methicillin-resistant coagulase-negative staphylococci (MRCNS) or multidrug-resistant bacteria. Through this study, it is recommended that the date of manufacture and expiration date must be indicated on the surface of disposable wet wipes designated as hygiene products, and clean uncontaminated wet wipes must comply with storage standards. In addition, the results are expected to contribute to the enhancement and improvement of hygiene management supervision, personal hygiene management, and an improvement in national health.

Characteristics of the Microbial Community Responding to the Vertical Distribution of TPH Concentrations in the Petroleum-Contaminated Site (유류오염지역 부지 내 TPH 수직 농도 분포에 따른 미생물 생태 특성)

  • Song, Soo Min;Moon, Hee Sun;Han, Ji Yeon;Shin, Jehyun;Jeong, Seung Ho;Jeong, Chan-Duck;Cho, Sunghyen
    • Journal of Soil and Groundwater Environment
    • /
    • v.27 no.spc
    • /
    • pp.51-63
    • /
    • 2022
  • In this study, the TPH(Total Petroleum Hydrocarbon) contamination and microbial ecological characteristics in petroleum-contaminated site were investigated through the correlation among the vertical TPH contamination distribution of the site, the geochemical characteristics, and the indigenous microbial ecology. The high TPH concentration showed in the vicinity of 3~4 m or less which is thought to be affected by vertical movement due to the impervious clay layer. In addition, the TPH concentration was found to have a positive correlation with Fe2+, TOC concentration, and the number of petroleum-degrading bacteria, and a negative correlation with the microbial community diversity. The microbial community according to the vertical distribution of TPH showed that Proteobacteria and Firmicutes at the phylum level were dominant in this study area as a whole, and they competed with each other. In particular, it was confirmed that the difference in the microbial community was different due to the difference in the degree of vertical TPH contamination. In addition, the genera Acidovorax, Leptolinea, Rugoshibacter, and Smithella appeared dominant in the samples in which TPH was detected, which is considered to be the microorganisms involved in the degradation of TPH in this study area. It is expected that this study can be used as an important data to understand the contamination characteristics and biogeochemical and microbial characteristics of these TPH-contaminated sites.

Assessing Groundwater Vulnerability Using DRASTIC Method and Groundwater Quality in Changwon City (DRASTIC과 지하수 수질에 의한 창원시 지하수 오염취약성 평가)

  • Hamm Se-Yeong;Cheong Jae-Yeol;Kim Moo-Jin;Kim In-Soo;Hwang Han-Seok
    • Economic and Environmental Geology
    • /
    • v.37 no.6 s.169
    • /
    • pp.631-645
    • /
    • 2004
  • This study assesses groundwater vulnerability to contaminants in industrial and residential/commercial areas of the city of Changwon, using DRASTIC technique and groundwater data. The DRASTIC technique was originally applied to situations in which the contamination sources are at the ground surface, and the contaminants flow into the groundwater with infiltration of rainfall. Mostly the industrial area has higher DRASTIC indices than the residential/commercial area. However, a part of the residential/commercial area having much groundwater production and great drawdown is more contaminated in groundwater than other industrial and the residential/commercial areas even if it has lowest DRASTIC indices in the study area. It indicates that groundwater contamination in urban areas can be closely related to excessive pumping resulting in a lowering of the water level. The correlation coefficient between minimum DRASTIC indices and the degree of poor water quality for 10 districts is as low as 0.40. On the other hand, the correlation coefficients between minimum DRASTIC indices and the groundwater discharge rate, and between minimum DRASTIC indices and well distribution density per unit area are 0.70 and 0.87, respectively. Thus, to evaluate the potential of groundwater contamination in urban areas, it is necessary to consider other human-made factors such as groundwater withdrawal rate and well distribution density per unit area as well as the existing seven DRASTIC factors.

Investigation of Use of Potable Water in Pusan and Kyung nam Area (부산 . 경남 일원에서의 음용수 이용 실태조사)

  • 김용관
    • Journal of Environmental Health Sciences
    • /
    • v.17 no.2
    • /
    • pp.27-34
    • /
    • 1991
  • This study was carried out to investigate the use habit of drinking water. The survey data were collected from 1,282 of students and 175 of officers from September 1990 to June 1991 in Pusan city and the South of Kyung sang-do. The use rate of tap water among respondents was 33.5%, and the reasons that the others avoid using tap water were having the risk of heavy metals contamination and unsanitary. The 80% of respondents have drunk spring water or bottled water within two or three years. The keeping method of them were 53% in the refrigerator, and 33% in the indoor. The use rate of water vessels what were equipped in mineral springs was 85%. It's advisable that the picking of mineral water be cautious within two or three days after rain. The holding rate of water purifier in respondents was about 20%, and it was purchased within one or two years and the reliance degree of that in users was below 50%.

  • PDF

Estimation of Effective Dose to Residents Due to Hypothetical Accidents During Dismantling of Steam Generator

  • Kyeong-Ju Lee;Chang-Lak Kim
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
    • /
    • v.21 no.2
    • /
    • pp.183-191
    • /
    • 2023
  • The potential impact of hypothetical accidents that occur during the immediate and deferred dismantling of the Kori Unit 1 steam generator has been comprehensively evaluated. The evaluation includes determining the inventory of radionuclides in the Steam Generator based on surface contamination measurements, assuming a rate of release for each accident scenario, and applying external and internal exposure dose coefficients to assess the effects of radionuclides on human health. The evaluation also includes calculating the atmospheric dispersion factor using the PAVAN code and analyzing three years of meteorological data from Kori NPP to determine the degree of diffusion of radionuclides in the atmosphere. Overall, the effective dose for residents living in the Exclusion Area Boundary (EAB) of Kori NPP is predicted, an it is found that the maximum level of the dose is 0.034% compared to the annual dose limit of 1 mSv for the general public. This implies that the potential impact of hypothetical accidents on human health discussed above is within acceptable limits.

A Study on the Degree of Contamination of Air and Materials in a Hospital (병원내 공기오염과 물품의 항균에 대한 상태조사)

  • 차옥주
    • Journal of Environmental Health Sciences
    • /
    • v.8 no.1
    • /
    • pp.25-30
    • /
    • 1982
  • The incidence of hospital infection has been seriously increased in the general hospital in recent years. This study was performed on hospital air and materials in a General Hospital in Seoul from June to December in 1980. The results were as follows: 1. Air sampling was done in multiple strategic areas by exposing standard petridishes for 5 minutes. There was a significant difference of airborne microbe between places. ($F._{99}$ = 3.2, p < 0.01). 2. The mean colony count was 8.6$\pm$6.2 colonies / plate / 5 minutes. 3. Gram stains of colony in air sampling were Gram (+) cocci 66.5%. Gram (+) rod 18.4%, Gram (-) cocci 1.3%, Gram (-) rod 8.7% Fungus 4.5%. 4. For the evaluation of sterilization of steam sterilizer and ethylene oxide gas sterilizer, biological monitoring were done by commercial spore strip. Positive culture was obtained in 2 out of 41 tests on 3 steam sterilizers, and in 3 out of 13 tests on ethylene oxide gas sterilizer. 5. Product sampling and culture were done for 2 kinds of disinfectants and 30 sets of various operation package or dressing materials. Positive culture was obtained in one disinfectants.

  • PDF

Harmonics Analysis of Leakage Current Waveforms and Surface Discharge for EPDM Polymer Insulator (EPDM 고분자 애자에 대한 표면방전 및 누설전류파형의 고조파분석)

  • 박재준;양태규
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.17 no.9
    • /
    • pp.1013-1021
    • /
    • 2004
  • This paper presents the results of spectrum analysis of leakage current waveforms on contaminated polymer insulators under fog conditions. This is a basic study on development of a monitoring for aging degree and surface pollution state using distortion rate(%) of 3rd and 5th harmonics according fundamental frequency[60Hz] under environment conditions. Arcing on the surface was always associated with distortion in the leakage current ; hence the low frequency components of the leakage current can be used as a means to evaluate the surface degradation. The results indicate that the distortion rate by spectrum analysis is available for the monitoring of contamination condition of EPDM Polymer insulators.

On the Design of the Latch Mechanism for Wafer Containers in a SMIF Environment

  • Lee, Jyh-Jone;Chen, Dar-Zen;Pai, Wei-Ming;Wu, Tzong-Ming
    • Journal of Mechanical Science and Technology
    • /
    • v.20 no.12
    • /
    • pp.2025-2033
    • /
    • 2006
  • This paper presents, the design of a latch mechanism for wafer containers in a standard mechanical interface environment. For an integrated circuits fabrication factory, the standard mechanical interfaced wafer container is an effective tool to prevent wafers from particle contamination during wafer storage, transporting or transferring. The latch mechanism inside the container door is used to latch and further seal the wafer container for safety and air quality. Kinematic characteristics of the mechanism are established by analyzing the required functions of the mechanisms. Based on these characteristics, a methodology for enumerating feasible latch mechanisms is developed. New mechanisms with one degree-of-freedom and up to five links are generated. An optimum design is also identified with respect to the criteria pertinent to the application. The computer-aided simulation is also built to verify the design.

Evaluation of the Speckle Noise in Optical Scanning Holography (광 스캐닝 홀로그래피와 스펙클 잡음에 의한 오염도 평가)

  • Kim, You Seok;Kim, Taegeun
    • Korean Journal of Optics and Photonics
    • /
    • v.25 no.3
    • /
    • pp.142-145
    • /
    • 2014
  • In this paper we record the complex hologram of a real object with optical scanning holography (OSH). We reconstruct the complex hologram using a numerical process, and then we evaluate the degree of contamination by speckle noise between the reconstruction of the complex hologram and the image captured by a CCD camera. We use the contrast of the speckle pattern for quantitative evaluation.