• Journal of Microbiology and Biotechnology
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• 제5권5호
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• pp.245-249
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• 1995

Expression of the adjacent but divergently transcribed glpD and glpE gene is positively regulated by cAMP-CRP. In this study, we constructed several mutants in which a CRP-binding site is placed at different distances upstream of the glpD promoter. The effect of the spacer length on transcription activation by cAMP-CRP was tested in vivo by $\beta$-galactosidase. The cAMP-CRP complex activated transcription from glpD when bound at a number of positions, all of which lay on the same face of the DNA helix, although the degree of activation varied with the length of the spacer. By contrast, the insertion of spacer length with non-integral turns of the DNA helix extremely inhibited the activation of transcription. The observed transcription activation by cAMP of the glpD promoter was influenced by the distance between the CRP binding site and the transcription start point.

• 약학회지
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• 제45권6호
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• pp.656-663
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• 2001

The quality of an intravenous immunoglobulin preparation (IVIG) is reflected by the degree of nonspecific activation of complements, the so-called anticomplementary activity (ACA). ACA of aggregates in IVIG was investigated using method by the European Pharmacopoeia and Clq-coated microtiter enzyme-linked immunosorbent assay (ELISA). Both the EP method and the ELISA method showed a dose response curve with the amount of complements bound increasing with the percentage content of aggregates in immunoglobulin standard. The correlation between the two tests was good (r=0.96, r=0.99). However, the correlation was not found when the ACA (EP method) of IVIG product was compared with its aggregate percentage. These results emphasize that the method of aggregate formation affects ACA and that estimation of the percentage distribution of aggregates by HPLC may not reflect ACA. In analysing WIG product for Clq binding activity test with the ELISA, the result by using Protein A-HRP correlated with aggregate percentage (r=0.84). But the correlation decreased (r=0.48) when the result used Protein A-AP(having poorer sensitivity than HRP) was compared with aggregate percentage. As a result, some variation between the two methods, due to differences in assay principles, is to be expected. However, ELISA technique has the advantage in that it is easier to perform, more precise and less subject to reagent variability, and is the more suitable screening method than HPLC analysis.

• 로봇학회논문지
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• 제15권3호
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• pp.212-220
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• 2020

This paper deals with a strategy of gain optimization for the kinematic control algorithm of a wire-driven surgical robot. The proposed controller consists of the closed-loop inverse kinematics with the back-calculation method. The closed-loop inverse kinematics has 18 PID control gains, and the back-calculation method has 6 gains. An efficient strategy is designed to optimize 18 values first and then the remaining 6 values. The optimal gain sets are searched under the step input with performance indices. In this gain optimization, the objective function is defined as the minimum value of signal-to-noise ratio of the performance indices for 6 DoF (Degree-of-Freedom) motion that is based on the Taguchi method, and the constraints are applied to obtain stable responses for each motion evenly. The gain sets obtained are verified by simulations using the test trajectories. In comparative results, the optimal gain value based on the performance index combined with ISE (integral of square error) and settling time showed the best control performance.

• 미생물학회지
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• 제27권3호
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• pp.297-303
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• 1989

Attempts to isolate the naturally occurring ultra-violet resistant bacteria from environmental sources were made. The isolates, designated No.29, 100, and 107, among numbers of bacterial isolates revealed a remarkable resistance to UV ray, whose degree of resistance in dose/response kinetics was comparable to that of an endospore-former, Bacillus subtilis. In a range of 100-300 $Jm^{-2}$/min of UV irradiation, the isolates exhibited 500-1000 fold resistance compated with E. coli. The isolated appeared to possiss cell-bound pigment of organge or crimson-red. The isolate 29 is spherical in pairs or tetrads, whereas the isolates 100 and 107 are rod. All are Gram-gositive bacteria and seemed to be non-endospore-bearer. A number of biochemical studies pursued on the isolates suggested that they are quite different to each other. Electron microscopic examination and the physiological characters of the isolate 29 suggested that this UV resistant spherical bacterium might be one species of Deinococcus, probably Deinococus radiophilus. Since there is no documents on UV resistant, Gram-positive, non-sporeformer bacillus so far, the isolates 100 and 107 might be turned out as new kinds of UV resistant bacteria occurring in nature by further investigation.

• 대한수학회논문집
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• 제33권1호
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• pp.85-101
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• 2018

Let S be a polynomial ring over a field K, with a monomial order ${\prec}$, and let I be an unmixed graded ideal of S. In this paper we study two functions associated to I: The minimum distance function ${\delta}_I$ and the footprint function $fp_I$. It is shown that ${\delta}_I$ is positive and that $fp_I$ is positive if the initial ideal of I is unmixed. Then we show that if I is radical and its associated primes are generated by linear forms, then ${\delta}_I$ is strictly decreasing until it reaches the asymptotic value 1. If I is the edge ideal of a Cohen-Macaulay bipartite graph, we show that ${\delta}_I(d)=1$ for d greater than or equal to the regularity of S/I. For a graded ideal of dimension ${\geq}1$, whose initial ideal is a complete intersection, we give an exact sharp lower bound for the corresponding minimum distance function.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권1호
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• pp.291-304
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• 2019

This paper proposes a dynamic-tiling-based bandwidth-efficient (DTBE) virtual reality (VR) streaming scheme. We consider 360-degree VR contents with multiple view points such as the front, back, upper, and bottom sides. At a given time, the focus of a client is always bound to a certain view among multiple view points. By utilizing this perspective, under our proposed scheme, tiles with high encoding rates are selectively assigned to the focused view where multiple view points consist of multiple tiles with different encoding rates. The other tiles with low encoding rates are assigned to the remaining view points. Furthermore, for reducing view adaptation delay, we design a novel rapid view adaptation mechanism that selectively delivers an I-frame during view point updates by using frame indexing. We implement the proposed scheme on a commercial VR test bed where we adopt the MPEG media transport (MMT) standard with high-efficiency video coding (HEVC) tile modes. The measurement-based experiments show that the proposed scheme achieves an average data usage reduction of almost 65.2% as well as average view adaptation delay reduction of almost 57.7%.

• 한국항공운항학회지
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• 제31권4호
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• pp.126-132
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• 2023

During the COVID-19 pandemic, there have been many changes in the aviation industry as a whole. Passenger traffic has decreased, but on the contrary, the international transport demand for cargo planes has never been higher. Due to their geopolitical location when flying to major cities around the world, Korea's international pilots are bound to have relatively more jet lag-causing routes than their American or European-based pilots. Through this study, we tried to analyze the degree of fatigue and fatigue weighting factors according to operating conditions for domestic pilots. As a result of the study, the following conclusions were drawn. Despite the variety of models, routes, and fatigue between individuals, it was found that the 3 Pilot operation route was concentrated as a representative fatigue route. Most of the individual fatigue improvement request sections showed higher fatigue as the night flight section was longer among the 3Pilot routes for more than 12 hours, and 43 out of 90 participants (Korean Air pilots) focused on 3Pilot (two captains and one pilot) as fatigue-intensive operating conditions. Therefore, it proposes an improvement in the system of applying 12-hour unification standards according to Korea's geopolitical international operating conditions and time difference standards.

• 한국ITS학회 논문지
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• 제19권6호
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• pp.163-179
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• 2020

플랫폼 기반의 택시서비스의 확대로 이용자의 이동성 및 편의성이 개선되고 있으나, 수익성의 문제로 공급 취약지역이 발생할 것으로 예상된다. 이에 따라 본 연구에서는 대중교통이 종료되는 심야시간을 중심으로 수요 공급의 불균형에 따른 택시 서비스의 공간적 취약성을 분석하였다. Hot-spot 분석결과 승차대비 공차량 및 승차대비 하차량의 경우 도시 외곽지역 및 주거밀집 지역에서 높게 도출되었고, 반대로 도심 및 부도심에서 낮게 나타나는 것을 확인하였다. 중심성 분석 결과 또한 Hot-spot 분석결과와 유사한 패턴으로 분석되었다. 이는 운전자의 관점에서 내향 대비 외향중심성이 낮은 지역은 다음 영업에 한계로 간접승차 거부의 문제가 발생할 수 있으며, 승객의 입장에서는 대기시간 증가로 이어질 것으로 예상된다. 이에 향후 수요공급의 불일치에 의한 공간적 취약성 개선이 필요할 것으로 판단되며, 본 연구의 결과를 기초자료로 활용 할 수 있을 것으로 기대된다.

• 한국산림과학회지
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• 제90권4호
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• pp.505-512
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• 2001

본 연구에서는 차기 임산물협상(林産物協商)의 관세인하(關稅引下)가 임산물시장에 미치는 영향을 검토하였고 그 영향을 최소화하기 위한 방안을 제시하였다. UR협상 결과와 뉴라운드협상 준비과정에서 나타난 양허범위(讓許範圍), 협상기준세율(協商基準稅率), 관세인하폭 등 쟁점사항에 기초하여 차기 임산물협상(林産物協商)의 관세인하(關稅引下)에 대한 시나리오를 작성하였고 관세인하(關稅引下)에 의한 시나리오별 영향은 임업연구원에서 개발한 국내임산물시장분석모델을 이용하여 추정하였다. 차기 임산물협상(林産物協商)의 관세인하(關稅引下)는 국내소비량 및 생산량보다 상대적으로 순수입량에 더 큰 영향을 마치는 것으로 추정되었다. 차기협상의 관세인하(關稅引下)가 합판시장 및 제품의 순수입량에 미치는 영향이 상대적으로 클 것으로 나타났으나 원목시장과 제품의 생산량 및 소비량에 미치는 영향은 1% 미만일 것으로 분석되었다. 관세인하(關稅引下)로 1차 가공제품의 국내생산량이 감소됨에 따라 원목의 국내소비량이 감소되고 이에 따라 원목의 국내생산량 및 수입량은 모두 감소될 것으로 추정되었다. 차기협상의 관세인하(關稅引下)에 대한 영향을 최소화하기 위해서는 협상기준세율(協商基準稅率)을 양허품목(讓許品目)의 경우에는 양허세율(讓許稅率)을, 미양허품목(未讓許品目)의 경우에는 현행실행세율(現行實行稅率)로 하고, 이행기간을 연장하고, 개도국의 지위를 유지하도록 노력하여야 할 것이다. 또한 WTO 규범내 합법적인 반덤핑, 상계관세제도(相計關稅制度) 및 긴급수입제한조치(緊急輸入制限措置) 등을 활용하는 것도 하나의 방안이 될 것이다.

• 생명과학회지
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• 제14권5호
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• pp.770-777
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• 2004

a-Tropomyosin (TM)의 아미노(N) 말단 구조의 중요성을 확인하기 위하여 N 말단에 알라닌 아미노산 잔기 하나를 첨가한 재조합 Ala-TM을 제조하였다. Ala-TM을 대장균에서 대량발현 시켜 정제한 후, N 말단이 아세틸화된 근육TM및 N말단에 알라닌-세린 잔기를 첨가한 AS-TM과 N말단이 비아세틸화된 TM등의 재조합 TM과 기능을 비교하였다. Ala-TM은 비아세틸화된 TM보다 액틴친화력이 현저히 증가했으나, 근육 및 AS-TM 보다는 약 3배정도 약하게 액틴에 결합하였다. 근육 TM, AS-TM,그리고 Ala-TM모두가 myosin 51의 농도가 낮을 때 ATPase 활성을 억제하였고 농도가 높을 때 촉진하였으나, 억제와 촉진의 정도는 서로 차이가 있었으며 비아세틸화된 TM은 억제하지 않았다. 이들 결과는 N말단 구조가 TM의 기능을 결정하는 중요한 요소임을 나타내며 TM의 온전한 기능을 위해서는 아세틸화된 N 말단이 필요하다는 것을 의미한다.