• Mycobiology
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• v.42 no.1
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• pp.46-51
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• 2014

A degenerated strain of Pleurotus eryngii KNR2312 was isolated from a commercial farm. Random amplified polymorphic DNA analysis performed on the genomic DNA of the normal and degenerated strains of this species revealed differences in the DNA banding pattern. A unique DNA fragment (1.7 kbp), which appeared only in the degenerated strain, was isolated and sequenced. Comparing this sequence with the KNR2312 genomic sequence showed that the sequence of the degenerated strain comprised three DNA regions that originated from nine distinct scaffolds of the genomic sequence, suggesting that chromosome-level changes had occurred in the degenerated strain. Using the unique sequence, three sets of PCR primers were designed that targeted the full length, the 5' half, and the 3' half of the DNA. The primer sets P2-1 and P2-2 yielded 1.76 and 0.97 kbp PCR products, respectively, only in the case of the degenerated strain, whereas P2-3 generated a 0.8 kbp product in both the normal and the degenerated strains because its target region was intact in the normal strain as well. In the case of the P2-1 and P2-2 sets, the priming regions of the forward and reverse primers were located at distinct genomic scaffolds in the normal strain. These two primer sets specifically detected the degenerate strain of KNR2312 isolated from various mushrooms including 10 different strains of P. eryngii, four strains of P. ostreatus, and 11 other wild mushrooms.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.33 no.2
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• pp.46-53
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• 2005

Compare to the large number of curved quadrilateral degenerated-shell elements, there are only a very few curved triangular degenerated-shell elements. Based on the assumed natural strain sampling scheme previously developed for a quadratic degenerated-shell element for linear analysis, this paper devises geometric non-linear six-node degenerated-shell element. The element can be curved and is only equipped with the standard nodal d.o.f.'s. Careful consideration has been exercised to circumvent various locking phenomena that plague degenerated-shell element. Numerical examples are presented to illustrate efficiency.

• Structural Engineering and Mechanics
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• v.2 no.1
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• pp.17-34
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• 1994

This paper proposes a new four node degenerated shell element. In the formulation of the new element, the assumed covariant shear strains are used to avoid the shear locking problem, and the assumed covariant membrane strains are applied to alleviate the membrane locking problem and also to improve the membrane bending performance. The assumed covariant strains are obtained from the covariant strain field defined with respect to the element natural coordinate system. This formulation enables us to obtain a shell element, which does not produce spurious singular modes, avoids locking phenomena, and excels in calculation efficiency. Several examples in this paper indicate that, despite its simplicity, the achieved accuracy and convergence are satisfactory.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.14 no.4
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• pp.481-494
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• 2001

An enhanced degenerated shell finite element (FE), which has been developed for inelastic analysis of reinforced concrete structures is described in this paper. Generally, Reissner-Mindlin (RM) assumptions are adopted to develop the degenerated shell FE so that transverse shear deformation effects is considered. However, it is found that there are serious defects such as locking phenomena in RM degenerated shell FE since the stiffness matrix has been overestimated in some situations. As remedies of locking phenomena, reduced integration, incompatible mode and assumed strain method have been used. Especially, the assumed strain method has been successfully used in many FEs. But contrarily, there is a few investigation on the performance of the assumed strains in the inelastic analysis of concrete structures. Therefore, shell formulation is provided in this paper with emphasis on the terms related to the stiffness matrix based on assumed strain method and microscopic concrete material model. Finally, the performance of the present shell element is tested and demonstrated with several numerical examples. From the numerical tests, the present result shows a good agreement with experimental data or other numerical results.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.9 s.180
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• pp.2292-2301
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• 2000

We propose an accurate and efficient estimation method of transverse shear stresses for analysis and design of laminated composite structures by 4-node quadrilateral degenerated shell elements. To get proper distributions of transverse shear stresses in each layer, we use 3-dimensional equilibrium equations instead of constitutive equations with shear correction factors which vary diversely according to the shapes of shell sections. Three dimensional equilibrium equations are integrated through the thickness direction with complete polynomial membrane stress fields, which are recovered by REP (Recovery by Equilibrium in Patches) recovery method. The 4-node quadrilateral degenerated shell element used in this paper has drilling degrees of freedom and shear stresses derived from assumed strain fields that are set up at natural coordinate systems. The numerical results demonstrate that the proposed estimation method attains reasonable accuracy and efficiency compared with other methods and FE analysis using 4-node degenerated shell elements.

• Steel and Composite Structures
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• v.17 no.6
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• pp.867-890
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• 2014

This paper deals with the geometric nonlinear bending analysis of laminated composite stiffened plates subjected to uniform transverse loading. The eight-noded degenerated shell element and three-noded degenerated curved beam element with five degrees of freedom per node are adopted in the present analysis to model the plate and stiffeners respectively. The Green-Lagrange strain displacement relationship is adopted and the total Lagrangian approach is taken in the formulation. The convergence study of the present formulation is carried out first and the results are compared with the results published in the literature. The stiffener element is reformulated taking the torsional rigidity in an efficient manner. The effects of lamination angle, depth of stiffener and number of layers, on the bending response of the composite stiffened plates are considered and the results are discussed.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.20 no.3
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• pp.353-364
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• 2007

In this paper, a nonlinear finite element analysis program NUCAS, which has been developed for assessment of ultimate pressure capacity and failure mode for nuclear containment building is described. Degenerated shell element with assumed strain method and low-order solid element with enhanced assumed strain method is adapted to microscopic material and elasto-plastic material model, respectively. Finally, the performance of the developed program is tested and demonstrated with several examples. From the numerical tests, the present results show a good agreement with experimental data or other numerical results.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.13 no.2
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• pp.209-220
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• 2000

For non-linear analysis of stiffened shell structures, the total Lagrangian formulation is presented based upon the degenerated shell element. Geometrically correct formulation is developed by updating the direction of normal vectors and taking into account second order rotational terms in the incremental displacement field. Assumed strain concept is adopted in order to overcome shear locking phenomena and to eliminate spurious zero energy mode. The post-buckling behaviors of stiffened shell structures are traced by modeling the stiffener as a shell element and considering general transformation between the main structure and the stiffener at the connection node. Numerical examples to demonstrate the accuracy and the effectiveness of the proposed shell element are presented and compared with references' results.

• Structural Engineering and Mechanics
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• v.17 no.6
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• pp.751-766
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• 2004

This paper extends the use of the hierarchic degenerated shell element to geometric non-linear analysis of composite laminated skew plates by the p-version of the finite element method. For the geometric non-linear analysis, the total Lagrangian formulation is adopted with moderately large displacement and small strain being accounted for in the sense of von Karman hypothesis. The present model is based on equivalent-single layer laminate theory with the first order shear deformation including a shear correction factor of 5/6. The integrals of Legendre polynomials are used for shape functions with p-level varying from 1 to 10. A wide variety of linear and non-linear results obtained by the p-version finite element model are presented for the laminated skew plates as well as laminated square plates. A numerical analysis is made to illustrate the influence of the geometric non-linear effect on the transverse deflections and the stresses with respect to width/depth ratio (a/h), skew angle (${\beta}$), and stacking sequence of layers. The present results are in good agreement with the results in literatures.

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.2
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• pp.354-362
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• 1995

The finite element modeling is used to study the buckling and postbuckling behavior of composite laminates with an embedded delamination. Degenerated shell element and rigid beam element are utilized for the finite element modeling. In the nonlinear finite element formulation, the updated Lagrangian description method based on the second Piola-Kirchhoff stress tensor and the Green strain tensor is used. The buckling and postbuckling behavior of composite laminates with a delamination are investigated for various delamination sizes, stacking sequences, and boundary conditions. It is shown that the buckling load and postbuckling behavior of composite laminates depend on the buckling model which is determined by the delamination size, stacking sequence and boundary condition. Also, results show that introduction of couplings can reduce greatly the buckling load.