• Journal of the Korean Society for Precision Engineering
• /
• v.12 no.4
• /
• pp.88-99
• /
• 1995

The demand for automatic manufacturing systems is increasing. Flexible Manufacturing System(FMS) is usually considered as a soluting for the shop floor automation. One of the difficulties in FMS is the communications problem. Since various machineries with different communications protocoles are included, applying a unified scheme is almost impossible. Therefore, a systematic approach is a key point to solve the communication problem. A cell is difined as an automation unit where closely related for a job reside together. A cell is a messenger between upper level computers and lower level machine equipment. In this research, the fonctions of the cell are defined to have more capabilities than conventional cells since a cell can be often a total manufacturing system is a small to medium sized factory. The cell conducts communications with different machines through the communications schemes established here. A set of software system has been developed according to the defined communication. The software has been tested for a simulation and real experiments for proof.

• Korean journal of applied entomology
• /
• v.32 no.4
• /
• pp.420-425
• /
• 1993

The programme of silkrnoth chorion protem synthetIc changes is autonomous i. e.,it is imple I mented WIth normal kinetics by follicles cultured in isolation in a defined tIssue culture medium. On the basis of protem synthetic profiles, 8 stages of chorwgenesis are defined. Detail a analysis of the proteins of chorion of Bombyx mandarina, reveals more than 17 components by eletrophoretic mobility and synthetic kinetics. We have defined three abbreviated synthetlc s stages, based on the pattern of protein synthesized at early, middle, and late synthetic stages

• Clinical and Experimental Reproductive Medicine
• /
• v.12 no.2
• /
• pp.65-69
• /
• 1985

1) Rabbit follicular oocytes from preovulatory follicles were cultured for 12 hr in vitro and fertilized in vivo by transferring the oocytes to the first foster-mother. 2) Two youngs were bron from transferred embryos from the first foster-mother to the second foster-mother. This demonstrates that in vitro cultured follicular oocytes are normal and they can develop into normal young born when transferred to the foster-mother. 3) A simple chemically defined culture medium, salt sol. with glutamine (2mM), which was developed by Bae and Foote(1975) proves fully good enough for rabbit follicular oocyte culture. We call this B-F medium. 4) Twelve hours culture in vitro of the rabbit follicular oocyte may be a proper culture time for further development.

• Journal of Information Processing Systems
• /
• v.11 no.2
• /
• pp.280-294
• /
• 2015

This paper presents the infectious watermarking model (IWM) for the protection of video contents that are based on biological virus modeling by the infectious route and procedure. Our infectious watermarking is designed as a new paradigm protection for video contents, regarding the hidden watermark for video protection as an infectious virus, video content as host, and codec as contagion medium. We used pathogen, mutant, and contagion as the infectious watermark and defined the techniques of infectious watermark generation and authentication, kernel-based infectious watermarking, and content-based infectious watermarking. We experimented with our watermarking model by using existing watermarking methods as kernel-based infectious watermarking and content-based infectious watermarking medium, and verified the practical applications of our model based on these experiments.

• Korean Journal of Animal Reproduction
• /
• v.19 no.2
• /
• pp.129-134
• /
• 1995

This study was carried out to investigate on the survival rate and in vitro developmental rate of bisected porcine embryos and immature oocytes by manipulator and micropipette. Bisected embryos and immature oocytes cultured for 1∼5 days in TCM 199 medium with 20% FCS. Survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The survival rate of bisected porcine embryos and oocytes were 26.1%, 22.7%, respectively. The survival rate of bisected embryos and oocytes was significantly lower than that of non-bisection embryos(62.5%). 2. The survival rate of bisected porcine embryos in cultured for 12, 24, 48, 72 hrs with 20% FCS＋TCM-199 medium were 26.9%, 19.2%, 19.2% and 11.5%, respectively. 3. The in-vitro developmental rate with and without zona pellucida of bisected porcine embryos by micromanipulator were 30.8%, 25.0%, respectively.

• Microbiology and Biotechnology Letters
• /
• v.24 no.3
• /
• pp.327-330
• /
• 1996

An amino acid antimetabolite named YS-460 was isolated from the culture filtrate of a newly isolated Actinomycetes identified as Streptomyces sp. Fermentation was carried out in the synthetic medium at 30$\circ$C for 5 days. Purification was done by ion exchange resin, active carbon, silica gel column chromatography and obtained 38 mg of pure active substance per liter of the broth. YS-460, an amino acid like substance, has the molecular formula of C$_{7}$H$_{11}$NO$_{3}$- Its structure determined to be furanomycin by spectral analysis. It is active against some bacteria on a chemically defined medium and reversed competitively by L-isoleucine and non-competitively by L-leucine and L-valine.

• Journal of the Korean Society for Precision Engineering
• /
• v.22 no.3 s.168
• /
• pp.137-145
• /
• 2005

The present research deals with structural analysis and optimal design of the heating drum of a hot-rolling press for medium density fiberboard. Stress concentration in the journal of the heating drum is analyzed by the submodel technique of the finite element method. The fatigue life under operating conditions is analyzed and evaluated by the stress-life theory. Shape optimal design problems, to minimize the maximum stress occurring in the journal, are formulated and shape parameters of the corner fillets of the journal are defined as the design variables. The problems are solved by the numerical optimization method and optimal shapes are found. The optimal designs are shown to be reliable in terms of the maximum stress and the fatigue life.

• Journal of the Korean Society for Precision Engineering
• /
• v.25 no.9
• /
• pp.59-70
• /
• 2008

Polynomial is used to optimize crown bowl shape of a marine medium speed diesel engine piston. The primary goal of this paper is that it's for an original design through a thermal stress and highest temperature minimum. Piston is modeled using solid element with 6 design variables defined the positional coordinate value. Global optimum of design variables are found and evaluated as developed and integrated with the optimum algorithm combining genetic algorithm(GA) and tabu search(TS). Iteration for optimization is performed based on the result of finite element analysis. After optimization, thermal stress and highest temperature reduced 0.68% and 1.42% more than initial geometry.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 1998.06b
• /
• pp.270-275
• /
• 1998

It was assumed that the voice of cattle is generated with the virtual white noise through the digital filter called the linear prediction filter, and filter parameters (prediction coefficients) were estimated by the maximum entropy method (MEM) , using the sound signal of the animal . The feature planes were defined by the pairs of two parameters selected appropriately from these parameters. The cattle voices were divided into three levels, that is the high, medium and low levels according to their total power equivalent to the variances of the sound signal . It was found that the straight lines could be used for recognizing tow cow and one calf for high level voices. For high and medium level voices, however, it was difficult or impossible to recognize individual cattle on the parameters planes.

• Journal of Applied Biological Chemistry
• /
• v.57 no.1
• /
• pp.61-63
• /
• 2014

Aged black garlic (ABG) was extracted with 20% ethanol and water (crude extracts) and fractionated into three categories (>10, 3-10, and <3 kDa). The effect of crude extract supplements on anti-My-10 hybridoma cell growth and IgG1 antibody production was investigated in suspension culture with a chemically defined protein-free medium. We observed that supplementation of ABG to the cell culture medium stimulated anti-My-10 hybridoma cell growth and production of IgG1 antibody, particularly with fractionated ABG of low molecular weight. The stimulation depended upon the concentration and the size of the fractionated ABG. We also found that the growth-promoting activity was not correlated with high antibody production. These results suggest that fractionated ABG is a novel and promising alternative as an animal cell culture supplement.