• Journal of Veterinary Science
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• v.21 no.2
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• pp.20.1-20.13
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• 2020

Actinobacillus pleuropneumoniae (APP) causes a form of porcine pleuropneumonia that leads to significant economic losses in the swine industry worldwide. The apxIBD gene is responsible for the secretion of the ApxI and ApxII toxins and the pnp gene is responsible for the adaptation of bacteria to cold temperature and a virulence factor. The apxIBD and pnp genes were deleted successfully from APP serotype 1 and 5 by transconjugation and sucrose counter-selection. The APP1ΔapxIBDΔpnp and APP5ΔapxIBDΔpnp mutants lost hemolytic activity and could not secrete ApxI and ApxII toxins outside the bacteria because both mutants lost the ApxI- and ApxII-secreting proteins by deletion of the apxIBD gene. Besides, the growth of these mutants was defective at low temperatures resulting from the deletion of pnp. The APP1ΔapxIBDΔpnp and APP5ΔapxIBDΔpnp mutants were significantly attenuated compared with wild-type ones. However, mice vaccinated intraperitoneally with APP5ΔapxIBDΔpnp did not provide any protection when challenged with a 10-times 50% lethal dose of virulent homologous (APP5) and heterologous (APP1) bacterial strains, while mice vaccinated with APP1ΔapxIBDΔpnp offered 75% protection against a homologous challenge. The ΔapxIBDΔpnp mutants were significantly attenuated and gave different protection rate against homologous virulent wild-type APP challenging.

• Journal of the Korea institute for structural maintenance and inspection
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• v.10 no.2
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• pp.185-190
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• 2006

This study carried out an evaluation of an oil tank that was defective in structure. This paper is divided into two sections which are analysis for the working process and suggestion for improving on the structure of the oil tank. As stated above, it has three major factors as to an improvement, such as the life cycle of structure (especially physical life cycle), and the many factors related to the duration years of the oil tank and analysis result. Based on this study, the improvement of oil tank was established. For example, the ground investigation needs before design and construction of oil tank, and constructing the oil tank by retaining wall type tank is good for maintenance. Also, it is important that the system for drainage is related to maintenance.

• The Korean Journal of Air & Space Law and Policy
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• v.27 no.2
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• pp.67-106
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• 2012

Today air traffic at the airport is complicated including a significant increase in the volume of air transport, so aviation accidents are constantly occurring. Therefore, we should newly recognize importance of the Air Traffic Safety, the core values of the Air Traffic. The location of airport that is the basic infrastructure of the air traffic and the security of safety for facilities and equipments are more important than what you can. From this dimension, I analyze the step-by-step safety factors that are taken into account in the airport development projects from the construction or improvement of the airport within the current laws and institutions and give my opinion on the enhancement of safety in the design and construction of airport. The safety of air traffic, as well as airport, depends on location, development, design, construction, inspection and management of the airport including airport facilities because we have to carry out the national responsibility that prevents the risk of large social overhead capital for many and unspecified persons in modern society through legislation regarding intervention of specialists and locational criteria for aviation safety from the planning stage of airport development. In addition, well-defined installation standards of airports and air navigation facilities, the key points of the airport development phase, can ensure the safety of the airport and airport facilities. Of course, the installation standards of airport and air navigation facilities are based on the global standard due to the nature of air traffic. However, to prevent the chaos for the safety standards in design, construction, inspection of them and to ensure the aviation safety, the safety standards must be further subdivided in the course of domestic legislation. The criteria for installation of the Air Navigation facilities is regulated most specifically. However, to ensure the safety of the operation for Air Navigation Facilities, performance system proved suitable for the Safety of Air Navigation Facilities must change over from arbitrary restrictions to mandatory restrictions and be applied for foreign producers as well as domestic producers. Of course, negligence of pilots and defective aircraft maintenance lead to a large portion of the aviation accidents. However, I think that air traffic accidents can be reduced if the airport or airport facility is perfect enough to ensure the safety. Therefore, legal and institutional supplement to prioritize the aviation safety from the stage of airport development may be necessary.

• Journal of the Society of Naval Architects of Korea
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• v.55 no.1
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• pp.66-74
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• 2018

In order to evaluate the precision degree of the blocks on the dock, the shipyards recently started to use the point cloud approaches using the 3D scanners. However, they hesitate to use it due to the limited time, cost, and elaborative effects for the post-works. Although it is somewhat traditional instead, they have still used the electro-optical wave devices which have a characteristic of having less dense point set (usually 1 point per meter) around the contact section of two blocks. This paper tried to expand the usage of point sets. Our approach can estimate the rework time to weld between the Pre-Erected(PE) Block and Erected(ER) block as well as the precision of block construction. In detail, two algorithms were applied to increase the efficiency of estimation process. The first one is K-mean clustering algorithm which is used to separate only the related contact point set from others not related with welding sections. The second one is the Concave hull algorithm which also separates the inner point of the contact section used for the delayed outfitting and stiffeners section, and constructs the concave outline of contact section as the primary objects to estimate the rework time of welding. The main purpose of this paper is that the rework cost for welding is able to be obtained easily and precisely with the defective point set. The point set on the blocks' outline are challenging to get the approximated mathematical curves, owing to the lots of orthogonal parts and lack of number of point. To solve this problems we compared the Radial based function-Multi-Layer(RBF-ML) and Akima interpolation method. Collecting the proposed methods, the paper suggested the noble point matching method for minimizing the rework time of block-welding on the dock, differently the previous approach which had paid the attention of only the degree of accuracy.

• Geophysics and Geophysical Exploration
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• v.21 no.1
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• pp.8-14
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• 2018

For the purpose of maintenance and prevention of earth fill dams against damage from natural hazards, automatic monitoring through various measuring instruments and resistivity survey has been carried out. Reservoirs and embankments have the structural vulnerability on the agricultural usages since most of them were built more than thirty years ago. The main aim to use monitoring method is to verify the safety and integrity of the dam. Resistivity survey can detect potential weaknesses, such as defective zones, anomalous seepages or internal erosion processes. Permanent resistivity monitoring systems were installed at a reservoir, which daily measurements have been taken every 6 hour. Using monitoring data for one year, anomalous seepage and structural defects were clarified for dam safety. Annual water level fluctuations are around 10 m. During their operation, reservoir dams are subject to a never-ending hydraulic load from the reservoir, which over the years may cause changes in the properties of the inner parts of the dam construction. Detailed analysis of the monitoring results was performed and showed that resistivities at most locations have been very stable over the full monitoring period excluding the effects of water fluctuation and seasons. To investigate the detectability of weak zone using the DC resistivity monitoring, numerical modeling with a simplified model for the drainage at a reservoir dam was also performed. The results showed that the seepage zone near drainage in a reservoir dam could be detected by resistivity response change.

• Journal of Korean Tunnelling and Underground Space Association
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• v.24 no.5
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• pp.411-429
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• 2022

Instrumentations are essential in NATM tunnels, however measuring instruments are installed and applied without performance verification procedures due to insufficient research on methods, procedures, regulations, etc. to verify the reliability of the measuring instruments. In this study, domestic and foreign regulations relating to the verification and calibration of instruments were investigated and necessities for accreditation standards were proposed. In order to identify the causes of the defects, an external inspection was performed on rock bolt stressmeter and rod extensometer, which are measuring instruments with relatively complex structures. For verifying the performance of these instruments, verification devices were developed that can load step-by-step and the causes of defects were identified in measuring instruments of nine domestic manufacturers. Through the performance test, a number of measuring instruments were found to be defective. It was important to test the performance of the instruments in the state of a finished product and accordingly performance inspection methods and procedures were proposed. The results of this study are expected to help preparing related regulations for verifying instrument performance and selecting instruments in the field.

• Tunnel and Underground Space
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• v.16 no.2 s.61
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• pp.109-134
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• 2006

Rocks undergo weathering processes influenced by changing in pressure-temperature condition, atmosphere, underground water, and rainfall. The weathering processes change physical and chemical characteristics of the rocks. Once the rocks are weathered, the characteristics of them are changed and, because of the changing, several disadvantages such as rock slope failures and underground water spouts are can occur. Before we cut a large rock slope, therefore, we must analyze current weathering conditions of rocks and predict weathering processes in the future. Through the results of such analyses, we can judge reinforcement works. In order to comply with such requests, chemical weathering sensitivity analysis which was analyzed from chemical weathering velocities and other characteristics of rocks has been applied in several prior construction works in Korea. But, It is defective to use directly in engineering fields because it was developed for soils(not rocks), it has too mny factors must be considered and the relationships between the factors are not clear, and it is hard to explain the weathering processes in engineering time range. Besides above, because it has been used for isotropic rocks, this method is hard to apply to anisotropic rocks such as sedimentary rocks. Acceding to studies from morphologists (e.g. Oguchi et al., 1994; Sunamura, 1996; Norwick and Dexter, 2002), time dependent strength reduction influenced by weathering shows a negative exponential function form. Appling this relation, one can synthesize the factors which influence the weathering processes to the strength reduction, and get meaningful estimates in engineering viewpoint. We suggest this weathering sensitivity characterization method as a technique that can explain time dependent weathering sensitivity characteristics through strength changes and can directly applied the rock slope design.