• Title/Summary/Keyword: deep-sea

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Marine Prokaryotic Diversity of the Deep Sea Waters at the Depth of 1500 m Off the Coast of the Ulleung Island in the East Sea (Korea) (울릉도 연안 수심 1500 m에 서식하는 해양미생물군집의 분포)

  • Kim, Mi-Kyung;Khang, Yongho
    • Korean Journal of Microbiology
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    • v.48 no.4
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    • pp.328-331
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    • 2012
  • Microbial diversity in the 1500 m depth sea waters off the coast of Ulleung island of the East Sea, Korea, was investigated. Genomic DNAs were extracted directly from the marine microbes filtered through ultramembrane filters. Pyrosequencing of 16S rDNAs of these microbes resulted in 13,029 reads, of which uncultured bacteria consisted of 54.1%, alphaproteobacteria 23.4%, and gammaproteobacteria 22.3%. Other classes such as flavobacteria, actinobacteria, and epsilonproteobacteria were distributed within 0.2% of total reads. Among the cultivable bacteria, it was found that Rhodobacteraceae family of alphaproteobacteria, Alteromonadaceae, Halomonadaceae, and Piscirickettsiaceae families of gammaproteobacteria were mostly distributed in the deep-sea waters.

A Preliminary Study comparing the Growth of Phytoplankton according to the Ratio of Deep and Surface Seawater (해양심층수와 표층수의 혼합비율에 따른 식물플랑크톤의 증식 변화에 대한 기초연구)

  • Kim, Ah-Ree;Lee, Seung-Won;Jung, Dong-Ho;Moon, Deok-Soo;Kim, Hyeon-Ju
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.4
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    • pp.373-379
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    • 2010
  • The artificial upwelling of deep seawater increases primary production. This study conducted a lab-scale experiment to investigate the growth of phytoplankton with the mixing ratio of deep and surface seawater. The chlorophyll content in the sample of pure deep seawater was highest, regardless of the phytoplankton groups. Nutrients contained in the deep seawater positively influenced the growth of phytoplankton. The optimum mixture to apply in an artificial upwelling system was a 1:1 ratio of deep and surface seawater. An experiment considering other environmental conditions, such as luminance and specific gravity, should be performed.

Fractionation of Heavy Metals by Early Diagenesis in Deep-sea core Sediments from the Korea Deep-sea Environmental Study (KODES) area, NE Equatorial Pacific (한국심해환경연구(KODES) 지역 표층 퇴적물 중 속성작용에 의한 금속의 분화)

  • Park, Sung-Hyun;Jung, Hoi-Soo;Park, Chan-Young;Lee, Kyeong-Yong;Kim, Ki-Hyun
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.4 no.3
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    • pp.215-225
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    • 1999
  • To study the vertical variations of major elements, trace elements and rare earth elements(REEs) contents in deep-sea sediments, six cores from Korea Deep-sea Environmental Study area(KODES) were analyzed. Topmost sediment layers of KODES area are divided into two Units; brown-colored and peneliquid Unit I and pale brown-colored and relatively solidified Unit II. Contents of major elements, REEs, Cu, Sr and Rb in each Unit are almost same, while contents of Mn, Ni and Co in Unit I are two or three times higher than those in Unit II. R-mode factor analysis represents that surface sediments are composed of alumino-silicate phase (AI-Ti-K-Mg-Fe-Rb-Ce), apatite phase (Ca-P-Cu-Sr-Trivalent Rare Earth Elements) and Mn-oxide phase(Mn-Ni-Co). Factor scores in silicate and apatite phases in each Unit are nearly same, whereas those in Mn-oxide phase in Unit I is higher than those in Unit II. While NilCu ratio in Unit I is two times higher than that in Unit II. We interprete the geochemical fractionation of Ni and Cu as a result that Ni can be remobilized in oxygen-depleted micro-environment in Units I and II and then easily reprecipitated in Unit I, while most of Cu supplied together with organic material is decomposed mostly in Unit I and sorbed into apatite.

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Comparison of Vane-shear Strength Measured by Different Methods in Deep-sea Sediments from KODOS area, NE Equatorial Pacific (북동태평양 KODOS지역 심해 퇴적물의 베인 전단강도 측정 방법에 따른 결과 비교)

  • Chi, Sang-Bum;Jung, Hoi-Soo;Kim, Hyun-Sub;Moon, Jai-Woon
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.4 no.4
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    • pp.390-399
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    • 1999
  • Siliceous and calcareous deep-sea core sediments were collected by a multiple corer from the KODOS (Korea Deep Ocean Study) area, northeast equatorial Pacific, to compare vane shear strengths measured by two different apparatuses and in different places of on-board and on-land laboratories. The apparatuses were 1) a hand-held vane with four blades of $2.0{\times}2.0$ cm, and 2) a motorized shear vane system with four blades of $1.0{\times}0.88$ attached on a rotational viscometer. Depth profiles of shear strengths of core samples determined by the apparatuses do not show any consistent difference. Also, there is no consistent difference between shear strength values measured on-board and on-land laboratories after storing the core samples for three months in a cold room by a motorized shear vane system. However, there are considerable differences between depth profiles of shear strengths measured at four different points (holes) of a core sample. Moreover, significant differences among the profiles of different tube samples from a multiple corer within a sampling station were observed. Heterogeneity in physical properties of each depth and sediment column, possibly due to bioturbation and bottom current flows, is likely responsible for the differences in the geotechnical properties.

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Effect of Deep Sea Water on Phase I, Phase II and Ornithine Decarboxylase. (Phase I, phase II 효소 및 ornithine decarboxylase에 미치는 해양심층수의 영향)

  • Shon, Yun-Hee;Kim, Mee-Kyung;Jang, Jung-Sun;Jung, Eun-Jung;Nam, Kyung-Soo
    • Journal of Life Science
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    • v.18 no.3
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    • pp.381-386
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    • 2008
  • Deep sea water was tested for cancer chemopreventive activity by measuring the activities of ${\beta}-$ naphthoflavone $({\beta}-NF)-induced$ cytochrome P 450 1A2 (CYP 1A2), quinone reductase (QR) and glutathione-S-transferase (GST), glutathione (GSH) levels, and ornithine decarboxylase (ODC) activity. The in vitro incubation of rat liver microsome with deep sea water (a hardness range of $100{\sim}1,000$) showed a hardness-dependent inhibition of CYP 1A2 activity. QR and GST activities were induced about $1.1{\sim}1.2$ fold with the treatment of deep sea water in murine hepatoma Hepa 1clc7 cells. In addition GSH levels were increased $1.3{\sim}1.4$ fold in a hardness range of $100{\sim}1,000$. The deep sea water showed 20.3 and 35.0% inhibition of 12-O- tetradecanoylphorbol-13-a-cetate (TPA)-induced ODC activity at hardness 800 and 1,000, respectively. Therefore, deep sea water is worth further investigation with respect to cancer chemoprevention or therapy.

Studies on the Korean Deep Sea Fishing Industry Administration -Analysis of present situation and prospects- (한국의 해외 원양어업 경영에 관한 연구 - 현황 분석과 전망 중심으로-)

  • 김우성
    • The Journal of Fisheries Business Administration
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    • v.1 no.2
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    • pp.1-22
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    • 1970
  • Our fisheries protucts industry has developed rapidly during past 10 years ; production was about twice: fihing fleets increased twice in number and 3 times in tonnage ; export was 5 times. Govermment is trying to develop deep sea fisheries in order to surmount the depression of coast fisheries. At present more than 270 deep sea fishing boats are working with superior skill to other country at the South Pacific, the Atlantic, the Indian, and the North Pacific Ocean. Our deep sea fisheries is consisted of the tuna long line and the trawler. The tuna long line of them has 230 crafts in 1969 ana the deep sea trawler has 40 crafts, too. Comparing to 1962, the number of the deep sea fishing boats has been increased highly to 54 times, 7.71 times average per year increasing rate. The rate of the tuna long line to the trawler at the end of 1969 shows 85:15, More than half of them are 100~200 (equation omitted), if we classify them according to (equation omitted) or boat craft. 70% of them has less than 5 years ships age. The Korean Marine Industry Development Corporation has more than 1/3 fishing fleets, with 91 crafts, if We consider it according to corporation. Considering it according to the financial resources, dependence upon foreign loan is as high as 88%. Catches was 74, 450 M/T ($24, 663, 000)at the end of November in 1969 and it was increased to 113.5 times in catch amount and 118 times in value, comparing to those of 1962. Considering it according to the ocean, the order is arranged to the Pacific, the Atlantic, and the Indian Ocean. The average production amount of each craft is 250~400 S/T a year. The result of export took up 1/3 of total fisheries product export with $ 22, 398, 000 at the end of november in 1969. Employee cost of fishing coast is 8% higher than other fishing. The profit is highest in our fisheries. Most of the products except the trawler fishing are sofa at the fishing grounds to the processing company, and they lose much money. They buy most of bait from Japan, giving $8~10 for 1C/S(10kg). Fish price is $390~520 according to the kind of fish for S/T at the fishing grounds, and the rapid frozen fishes brought to Japan are about $ 800 for S/T. There is much difference. in price. Problems 1. Want of self capital. 2. To get the refrigeration boats enough. 3. International Fishing Regulation. 4. To get high price and to secure consuming grounds. 5. To get home-production of bait. 6. To exploit new fishing grounds. 7. To get larger boats. 8. To get mariner enough. When the problems mentioned above are solved, the Deep Sea Fisheries of oun courtry will be developed more largely.

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Deep Water Wave Model for the East Sea (東海에서의 파랑추산을 위한 심해파랑모형에 대한 연구)

  • Yoon, Jong-Tae
    • Journal of Ocean Engineering and Technology
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    • v.13 no.2 s.32
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    • pp.116-128
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    • 1999
  • A deep water wave prediction model applicable to the East Sea is presnted. This model incorporates rediative transter of energy specrum, atmospheric input form the wind, nonlinear interaction, and energy dissipation by white capping. The propagation scheme by Gadd shows satisfactory results and the characteristics of the nonlinear interaction is simulated well by discrete interaction approximatiion. The application of the model to the sea around the Korean Peninsula shows reasonable agreement with the observation.

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Screening and Characterization of Psychrotrophic, Lipolytic Bacteria from Deep-Sea Sediments

  • Zeng, Xiang;Xiao, Xiang;Wang, Peng;Wang, Rengping
    • Journal of Microbiology and Biotechnology
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    • v.14 no.5
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    • pp.952-958
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    • 2004
  • Of 23 psychrotrophic bacteria isolated from the west Pacific deep-sea sediments, 19 were assigned to the $\gamma$-Proteobacteria, 3 to the <$\beta$-Proteobacteria, and 1 to the Gram-positive bacteria, as determined by their 16S rDNA sequences. Ten psychrotrophs, affiliated to the Psychrobacter, Pseudoalteromonas, and Pseudomonas genera in the $\gamma$-Proteobacteria group, were screened for lipolytic bacteria. The majority of the lipolytic isolates had growth temperatures between 4-$30^\circ{C}$, and all of them were neutrophilic, aerobic, or facultatively anaerobic, and some were able to produce multiple kinds of ectohydrolytic enzymes. The deep-sea strains Psychrobacter sp. wp37 and Pseudoalteromonas sp. wp27 were chosen for further lipase production analysis. Both strains had the highest lipase production when grown at 10 to $20^\circ{C}$; their highest lipase production occurred at the late-exponential growth stage; and the majority of the enzymes were excreted to the outside of the cells. Lipases from both strains had the same optimal reaction temperature and pH (20-$30^\circ{C}$, pH 7-8) and could retain about 60% of their highest activity at $4^\circ{C}$. Furthermore, SDS-PAGE and an in-gel activity test showed that they had the same high molecular mass of about 85 kDa.

Effect of Deep Sea Water Seed Priming on the Growth of Rice (Oryza sativa L.) Seedlings

  • Yoon Byeong-Sung;Shrestha Surendra Lal;Kang Won-Hee
    • Korean Journal of Plant Resources
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    • v.19 no.6
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    • pp.687-691
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    • 2006
  • This experiment was conducted on rice (cv. 2005 Thaoi) seeds to study whether priming with deep sea water (DSW) results in enhancement of seed emergence and seedling growth and to identify the optimum concentration of Deep Sea Water (DSW) for priming. Two experiments were conducted subsequently. In experiment 1, four concentrations of the DSW (10%, 20%, 30% and 40%), and in experiment 2, five concentrations of DSW (10%, 15%, 20%, 25% and 30%) were prepared and seeds were primed for 24 hours duration at $25^{\circ}C$. Beside this, hydro priming with plain water was also included as a control. Experiments were laid out in Completely Randomized Design (CRD) with three replications. Result showed that 20% DSW seed priming treatment had improved the emergence, seedling height, number of roots and root length as compare to other with DSW or without DSW treatments. Beyond 20% DSW priming (i.e. 25%, 30% and 40%) were not suitable for priming the seed. On the basis of seedlings growth parameters; emergence, seedling height, root number and length, and shoot root ratio, 20% DSW priming was the best priming treatment.

Effect of Deep Sea Water on Seed Germination, Photoperiod and Temperature on the Growth and Flowering of Buckwheat Species

  • Briatia, Xoxiong;Hong, Soon-Kwan;Sung, In-Je;Chang, Kwang-Jin;Park, Byoung-Jae;Park, Cheol-Ho
    • Korean Journal of Plant Resources
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    • v.25 no.3
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    • pp.323-328
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    • 2012
  • This paper describes the potential use of deep sea water to stimulate seed germination in both common and Tartary buckwheat. Treatment of 10% deep sea water at $25^{\circ}C$ would slightly enhance germination of buckwheat seeds compared to non-DSW treatment and other temperature. In this study, the significant effects of photoperiod and temperature on seedling growth were also found in the HL treatment for the number of leaf, plant height, and plant fresh weight and LL treatment for root length and leaf size. Common buckwheat (Suwon No.1) showed higher rate (93%) of flowering plants in the HS and LL (93% of flowering rates) than those revealed in the HS and LS treatment, while the low percentage(67%) of plant flowering plants was shown in the LS treatment. All plants (100%) of a Korean landrace, Ahndong-jaerae showed flowers in the HS and LS treatment. HL and LL treatment status did not occur in the plant's flowering. Any Tartary buckwheat (KW45) plant did not yet flowered when it was 21 days-old.