Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Deep Sea Water on Phase I, Phase II and Ornithine Decarboxylase.

Phase I, phase II 효소 및 ornithine decarboxylase에 미치는 해양심층수의 영향

  • Shon, Yun-Hee (Regional Innovation System, Intractable Diseases Research Center, Dongguk University) ;
  • Kim, Mee-Kyung (Regional Innovation System, Intractable Diseases Research Center, Dongguk University) ;
  • Jang, Jung-Sun (Regional Innovation System, Intractable Diseases Research Center, Dongguk University) ;
  • Jung, Eun-Jung (Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine, Dongguk University)
  • 손윤희 (동국대학교 지역연고산업진흥사업단, 난치병한양방치료연구센터) ;
  • 김미경 (동국대학교 지역연고산업진흥사업단, 난치병한양방치료연구센터) ;
  • 장정선 (동국대학교 지역연고산업진흥사업단, 난치병한양방치료연구센터) ;
  • 정은정 (동국대학교 의과대학 약리학교실) ;
  • 남경수 (동국대학교 의과대학 약리학교실)
  • Published : 2008.03.31
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Abstract

Deep sea water was tested for cancer chemopreventive activity by measuring the activities of ${\beta}-$ naphthoflavone $({\beta}-NF)-induced$ cytochrome P 450 1A2 (CYP 1A2), quinone reductase (QR) and glutathione-S-transferase (GST), glutathione (GSH) levels, and ornithine decarboxylase (ODC) activity. The in vitro incubation of rat liver microsome with deep sea water (a hardness range of $100{\sim}1,000$) showed a hardness-dependent inhibition of CYP 1A2 activity. QR and GST activities were induced about $1.1{\sim}1.2$ fold with the treatment of deep sea water in murine hepatoma Hepa 1clc7 cells. In addition GSH levels were increased $1.3{\sim}1.4$ fold in a hardness range of $100{\sim}1,000$. The deep sea water showed 20.3 and 35.0% inhibition of 12-O- tetradecanoylphorbol-13-a-cetate (TPA)-induced ODC activity at hardness 800 and 1,000, respectively. Therefore, deep sea water is worth further investigation with respect to cancer chemoprevention or therapy.

동해에서 취수한 해양심층수의 암예방 효능을 얄아보고자 암발생 억제물질의 생화학적 표식자(biochemical markers)인 CYP 1A2 활성, phase II 효소인 QR과 GST의 활성, GSH 함량 및 ODC 활성에 미치는 영향을 살펴보았다. 해양심층수는 암의 개시단계(initiation)를 유발시키는 것으로 알려진 CYP 1A2 활성을 경도의존적으로 저해시켰다. 해양심층수를 Hepa 1clc7 세포에 경도별$(100{\sim}1,000)$로 처리하였을 때 phase II 생체 해독효소인 QR과 GST의 활성은 최대 20%의 증가를 나타내었고, 외부의 독성물질이나 대사산물로부터 세포를 보호하는 역할을 하는 GSH의 함량은 $26{\sim}40%$의 증가를 나타내었다. 그리고 발암과정의 촉진/진행단계에 관여하는 ODC의 활성은 해양심층수의 경도 800과 1,000에서 20%와 35%의 저해율을 나타내었으며, 경도 1,000을 처리한 군에서는 양성대조군인 DFMO와 같은 저해율을 나타내었다. 그러므로 본 연구 결과에 의하면 동해 해양심층수는 발암과정과 관련된 개시 및 촉진/진행단계를 저해시켜 암예방 효능을 나타낼 것으로 사료된다.

Keywords

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