• The Plant Pathology Journal
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• 제15권1호
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• pp.8-13
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• 1999

Hypersensitive cell death of plants during incompatible plant-pathogen interactions is one of the efficient defense mechanisms of plants against pathogen infections. For better understanding of the molecular mechanisms involved in the plant hypersensitive response (HR), TMV-induced biotic plant cell death and CuSO4-induced abiotic plant cell death were compared in terms of expression patterns of ten different defense-related genes as molecular markers. The genes include five pathogenesis-related protein genes, two plant secondary metabolite-associated genes, two oxidative stress-related genes and one wound-inducible gene isolated from tobacco. Northern blot analyses revealed that a same set of defense-related genes was induced during both biotic and abiotic cell death but with different time and magnitude. The expression of defense-related genes in tobacco plants was temporarily coincided with the time of cell death. However, when suspension cell cultures was used to monitor the expression of defense-related genes, different patterns of the gene expression were detected. This result implies that three are common and, in addition, also different branches of signaling pathways leading to the induced expression of defense-related genes in tobacco during the pathogen- and heavy metal-induced cell death.

• BMB Reports
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• 제47권9호
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• pp.488-493
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• 2014

Adaptor protein FADD forms the death inducing signaling complex (DISC) by recruiting the initiating caspases-8 and -10 through homotypic death effector domain (DED) interactions. Cellular FLICE-inhibitory protein (c-FLIP) is an inhibitor of death ligand-induced apoptosis downstream of death receptors, and FADD competes with procaspase-8/10 for recruitment for DISC. However, the mechanism of action of FADD and c-FLIP proteins remain poorly understood at the molecular level. In this study, we provide evidence indicating that the death effector domain (DED) of FADD interacts directly with the death effector domain of human c-FLIP. In addition, we use homology modeling to develop a molecular docking model of FADD and c-FLIP proteins. We also find that four structure-based mutants (E80A, L84A, K169A and Y171A) of c-FLIP DEDs disturb the interaction with FADD DED, and that these mutations lower the stability of the c-FLIP DED.

• Clinical and Experimental Reproductive Medicine
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• 제41권3호
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• pp.97-107
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• 2014

The placenta is a temporary fetomaternal organ capable of supporting fetal growth and development during pregnancy. In particular, abnormal development and dysfunction of the placenta due to cha nges in the proliferation, differentiation, cell death, and invasion of trophoblasts induce several gynecological diseases as well as abnormal fetal development. Autophagy is a catalytic process that maintains cellular structures by recycling building blocks derived from damaged microorganelles or proteins resulting from digestion in lysosomes. Additionally, autophagy is necessary to maintain homeostasis during cellular growth, development, and differentiation, and to protect cells from nutritional deficiencies or factors related to metabolism inhibition. Induced autophagy by various environmental factors has a dual role: it facilitates cellular survival in normal conditions, but the cascade of cellular death is accelerated by over-activated autophagy. Therefore, cellular death by autophagy has been known as programmed cell death type II. Autophagy causes or inhibits cellular death via the other mechanism, apoptosis, which is programmed cell death type I. Recently, it has been reported that autophagy increases in placenta-related obstetrical diseases such as preeclampsia and intrauterine growth retardation, although the mechanisms are still unclear. In particular, abnormal autophagic mechanisms prevent trophoblast invasion and inhibit trophoblast functions. Therefore, the objectives of this review are to examine the characteristics and functions of autophagy and to investigate the role of autophagy in the placenta and the trophoblast as a regulator of cell death.

• 성인간호학회지
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• 제22권3호
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• pp.329-341
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• 2010

Purpose: The purpose of this study was to understand the meaning of death experienced by medical and nursing students through end-of-life care practice. Methods: Data were collected by in-depth interviews with twelve (six nursing and six medical) students. Conventional qualitative content analysis was used to analyze the data. Results: This findings were analyzed in three areas: 'feeling from the word of death', 'color association of death', and 'relation between life and death'. Results were three major themes and sixteen categories from the analysis. Three major themes include 'reality of uncertain death', 'have to leave, and 'new perception about death'. Sixteen categories include 'being well', 'fear', 'unknown', 'boundless', 'being with', 'out of sight', 'new start', 'go back to', 'place going by itself', 'place to meet with', 'being transformed', 'a sense of futility', 'the same point', 'a different point', 'continuous line', and 'a crossroad'. Conclusion: The findings suggest a number of themes that nursing and medical students reported about the end of life experiences that could be explored as a way of improving end of life care.

• 성인간호학회지
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• 제26권5호
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• pp.584-591
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• 2014

Purpose: The aim of this study was to explore the factors contributing to death anxiety among elders through family support, ageism experience, loneliness and helplessness. Methods: The participants were 155 elders who lived in S city. The data were collected through a self-reported questionnaire in elders over age 65. In addition, their levels of death anxiety, family support, ageism experience, loneliness, helplessness and death anxiety were measured using a likert scale Data analysis using a t-test, one-way ANOVA, Pearson correlation and multiple regression analysis with the SPSS 20 program. Results: Significant differences were found in the levels of perceived death anxiety between the variables affecting death anxiety in elders. Pearson's Correlation were found family support (p<.001), ageism experience (p<.001), loneliness (p<.001) and helplessness (p<.001) with death anxiety. Conclusion: It is concluded that such variables should be considered for decreasing death anxiety by family support, ageism experience, loneliness and helplessness in elders.

• 의학교육논단
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• 제20권3호
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• pp.150-155
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• 2018

End-of-life care competencies have been perceived as important and essential, so it has been suggested that end-of-life care be studied in undergraduate medical education. However, end-of-life care curriculum has mostly focused on acquisition of knowledge and skills rather than attitudes. Therefore, we aimed to explore whether education about death affects medical students' attitudes towards care for dying patients and perception of death anxiety, meaning in life, and self-esteem. A total of 15 first- or second-year medical students were surveyed with questionnaires before and after completing a 6-week death education course. Paired data analysis showed that participants' attitudes towards caring for terminally ill patients and their caregivers improved significantly (t=-2.84, p=0.013) with an effect size of 0.73. In contrast, no significant changes were found in death anxiety, meaning in life, or self-esteem. All participants agreed that formal teaching about death and dying must be encouraged in medical schools. Our results suggest that death education may positively influence attitudes towards end-of-life care. Although replication with larger samples is necessary, this preliminary finding may support the importance of developmentally appropriate end-of-life care education in medical schools.

• 가정간호학회지
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• 제20권1호
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• pp.63-71
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• 2013

Purpose: This study was performed to identify nursing college students' attitudes towards death and was conducted as a pre-investigation to prepare them for coping with terminally ill patients in their clinical practice. Methods: The subjects consisted of 321 nursing students. Their attitudes towards death were measured by using the Collett-Lester Fear of Death Scale(CLFODS). The data obtained were analyzed by using the one-wayANOVA and Duncan's multiple test using the SAS 9.2 soft ware program. Results: The mean CLFODS score was 2.99. Among the 4 subscales, there were significant differences among the students with respect to grade, indirect experience of death, social isolation, daily life stress, and suicidal ideation in the past year. Conclusion: A negative attitude towards death was observed in the psychosocial characteristics, with indirect experience of death, social isolation, daily life stress, and suicidal ideation in the past year. It is necessary to educate students on how to care for dying patients before clinical practice. This education can be carried out by developing a death education program after considering the above mentioned psychosocial characteristics noted in this study.

• 동서간호학연구지
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• 제25권1호
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• pp.26-32
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• 2019

Purpose: The purpose of this methodological study was to examine the validity and reliability of a translated Korean version of Templer's death anxiety scale among older adults. Methods: The translated Korean scale was administered to 203 older people who aged over 65 years. Exploratory factor analysis was utilized to assess the factor structure of the scale. Internal consistency of the scale was determined using Cronbach's ${\alpha}$ and Guttman's split-half coefficient. Results: Factor loadings of the scale ranged from .43 to .86. The scale showed good internal consistencies of both total scale (13 items, ${\alpha}=.83$) and three sub-scales: meaning of death (6 items, ${\alpha}=.77$), death-related event (5 items, ${\alpha}=.72$), and time of death (2 items, ${\alpha}=.80$). The concurrent validity compare with Fear of Death Scale was significant. The Cronbach's alpha and Guttman's split-half coefficient were .83 and .80, respectively. Conclusion: The findings of this study demonstrate that the Korean version of Templer's death anxiety scale had satisfactory validity and reliability to measure death anxiety among Korean older people.

• BMB Reports
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• 제56권3호
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• pp.166-171
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• 2023

Monocytes are peripheral leukocytes that function in innate immunity. Excessive triglyceride (TG) accumulation causes monocyte death and thus can compromise innate immunity. However, the mechanisms by which TG mediates monocyte death remain unclear to date. Thus, this study aimed to elucidate the mechanisms by which TG induces monocyte death. Results showed that TG induced monocyte death by activating caspase-3/7 and promoting poly (ADP-ribose) polymerase (PARP) cleavage. In addition, TG induced DNA damage and activated the ataxia telangiectasia mutated (ATM)/checkpoint kinase 2 and ATM-and Rad3-related (ATR)/checkpoint kinase 1 pathways, leading to the cell death. Furthermore, TG-induced DNA damage and monocyte death were mediated by caspase-2 and -8, and caspase-8 acted as an upstream molecule of caspase-2. Taken together, these results suggest that TG-induced monocyte death is mediated via the caspase-8/caspase-2/DNA damage/executioner caspase/PARP pathways.

• 호스피스학술지
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• 제8권1호
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• pp.13-28
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• 2008

목적: 본 연구는 학령기 아동의 죽음인식에 대한 탐색적 연구를 통하여 그들의 죽음인식에 대한 주관적 구조와 유형을 탐색하여 학령기 아동에게 죽음인식을 이해하며 효과적인 죽음준비교육 프로그램을 개발 시행하기 위한 기초자료를 제공하고자 시도하였다. 방법: 학령기 아동의 죽음인식에 관한 주관적인 구조와 유형을 탐색할 수 있는 Q방법론을 사용하였다. Q-모집단은 학령기 아동 20명을 대상으로 중립적 면담과 개방형 질문지를 이용하고 문헌연구를 통하여 총 132개의 진술문을 수집하였으며. Q표본은 비 구조화된 방법을 통해 23개의 진술문(Q-표본)을 도출하였다, P표본은 학령기 아동 31명(8세-13세)이었으며 Q카드를 이용하여 Q-분류를 실시하였으며, 수집된 자료는 PC QUANL 프로그램을 사용하여 분석하였다. 결과: 학령기 아동의 죽음인식 유형은 5개의 유형으로 분류되었다. 제 1 유형은 기능적형으로 죽음의 구성요소인 비역행성, 보편성, 비기능성, 인과성에 대한 주관적 인식이 두드러진 유형이었다. 제 2 유형은 사후 세계형으로 기독교의 종교적 배경을 가진 아동들과 직계가족의 죽음을 경험을 했으며 사후세계에 대하여 강한 내세 중심의 죽음인식이 두드러진 유형이었다. 제 3 유형은 종교형으로 죽어서도 가족과 친구들을 지켜볼 수 있다는 믿음이 강하기 때문에 사후세계에 대한 긍정적인 믿음이 확실한 유형이었다. 제 4 유형은 공포형으로 죽음에 대한 공포가 타 유형에 비하여 강한 유형이었다. 제 5 유형은 현실형으로 좋은 죽음에 대한 인식에 강한 긍정적인 동의를 보이는 유형이었다. 결론: 본 연구결과가 주는 간호학적 의의는 다음과 같다. 간호실무면에서 학령기 아동의 죽음인식의 주관성을 이해하는데 있어서 기존의 문헌이나 연구에서 중점적으로 제시하고 있는 죽음의 구성요소에 대한 이해의 차원에서 좀 더 확대되어 학령기 아동이 인지하는 죽음의 정의, 사후세계, 좋은 죽음에 대한 인지수준을 이해하고 유형별 특성에 따라 학령기 아동들의 효과적인 죽음준비교육 프로그램을 개발하는데 유용한 자료로 사용하여, 청소년기에 나타나는 죽음 경시풍조 및 만연된 자살을 예방하고 올바른 삶을 살아갈 수 있도록 밑거름이 되는 역할을 담당 할 것이다. 간호이론 면에서 학령기 아동의 죽음인식의 주관적 구조와 유형별 특성을 규명하고 탐색함으로써 학령기 아동의 죽음인식 모델 구축과 나아가 생명존중의 유용한 자료로 활용될 수 있다. 간호연구 면에서 학령기 아동에게 유형별로 긍정적인 죽음인식과 더 나아가 생명 존중의 측면에서 심리사회적 간호를 제공하기 위한 도구의 개발과 간호중재 전략의 효과를 규명하는 연구에 기여할 수 있다.