• Horticultural Science & Technology
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• v.34 no.6
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• pp.830-839
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• 2016

Germination of Hemerocallis seeds as influenced by cold stratification at $5^{\circ}C$ (CS) and maturity of seeds evaluated using X-ray imaging has not been well investigated. Seeds of H. lilioasphodelus, H. citrina, H. citrina 'April Flower', and H. minor collected from China and H. thunbergii collected from Korea were germinated at $20^{\circ}C$ without pre-temperature treatment, while H. hongdoensis, H. dumortieri, H. minor, and H. vespertina seeds were treated with CS. Harvesting 'Stella de Oro' capsules at 35-40 days after anthesis yielded mature seeds with well-developed embryo and cotyledons analyzed by X-ray images with a 92% germination in 17 days after sowing. Seeds of H. thunbergii and H. citrina germinated in less than 13 days without CS; two weeks of CS did not accelerate seed germination. Seeds of H. hongdoensis germinated in 24 days when seeds were stored at $25^{\circ}C$ without CS and in less than 27 days when cold stratified. Therefore, 'Stella de Oro' capsules should be harvested at 35-40 days after anthesis to harvest mature seeds. Cold stratification is not required to accelerate seed germination in the Hemerocallis taxa evaluated in this study.

• Korean Journal of Veterinary Research
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• v.33 no.2
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• pp.277-283
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• 1993

In the present study, observations were made on the life-history of Lymnaea viridis under laboratory conditions, involving incubation period of the eggs and their hatching rate, shell length of the newly hatched snails, sexual maturity, size of the snails when the snail produced the first egg-mass, the number of eggs in each egg-mass, egg-laying, ovipostion, growth rate of the snails, and longevity of the snail. At temperatures between $19.8^{\circ}C$ to $22.5^{\circ}C$, incubation period of the eggs occupied 10~12 days, and after beginning of hatching, all young snails emerged completely from the egg-mass within 5 days. The hatching rate was 88%. The average shell length of the newly hatched snails was about 0.064cm. The rate of growth was extraordinarily rapid under good laboratory conditions. When two snails were reared in one culture vessel($20{\times}15{\times}5cm$) with blue-green algae at about $22^{\circ}C$, snail growth was optimal, taking 37 days to reach 1.2cm in shell length. Sexual maturity reached in about 19 days. The size of the snails at sexual maturity was $0.78{\pm}0.05cm$ in length and $0.47{\pm}0.04cm$ in width. The first egg-masses produced were $0.59{\pm}0.22cm$ in length and $0.34{\pm}0.08cm$ in width, and contained 7~38 eggs. The eggs are usually laid in water. The egg-laying was affected by food and temperature. Snails fed with blue-green algae at about $22^{\circ}C$ produced larger egg-masses than the snails fed with fish food at about $26^{\circ}C$. Under conditions of continuous activity and growth, the maximum expectation of life appears to be 109~350(mean 230) days. And the shell length of snails at death were 1.39~1.64cm.

• International Journal of Industrial Entomology and Biomaterials
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• v.47 no.1
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• pp.51-62
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• 2023

The advantages of silkworms as functional foods are well known and various products are being developed. In general, silkworms sold in the market include silkworm powder (3 days of fifth instars) and SukJam (7 days or more of fifth instars), In other words, product classification is made according to the maturity of the fifth instar silkworms. In this study, we analyzed the gene expression changes in the fifth instar silkworms and attempted to validate the use of deregulated genes in maturity analysis. After rearing BaekokJam, transcriptome analysis was performed on days 1, 3, 5, and 8 days of the fifth instar, and differentially expressed genes showing differences at each period were selected. Of the 31,841 contigs analyzed, 4012 contigs were identified with a log2 fold change of two or more between 5 and 8 days of the fifth instar. RT-PCR was performed for 18 contigs, which showed increased or decreased expression, but in c127159, c97909, c96974, c119920, c42251, and c80216 showed clear differences. To identify SukJam, a combination of the contigs c127159 (180 bp), c97909 (143 bp), and c80216 (120 bp) was amplified. Taken together, these results suggest that the harvest time of silkworms can be determined using gene expression pattern analysis.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.1
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• pp.57-63
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• 1994

We use a variety of methods to determine the optimum time for havesting corn for silage. In addition, adequate dry matter for silage must be considered along with maturity stage. The objective of this study was to evaluate using the kernel milk line to determine when to harvest com for silage in 1992 on the Livestock Experimental F m , Keiymung Junior College at Keongsan, Keongsangbukdo. Four hybrids were field grown and com plants were harvested at various stages of kernel development so that kemel milk line movement could be analyzed whilc the corn was in the premature stages. As the plants approached maturity, the ears were collected from each of the hybrids and the position of the milk line wa5 noted. Then the whole plants were chopped and the content of DM was determined. The milk line was a readily identifiable feature of maturing com kemels. We focused on the five development \tage\. The fint was "soft dough". The second was "dent". The third wa, "75% milk". and the fourth wa5 "half milk". The half milk occurs when the milk line is positioned falf way down the kemel face. and the final stages win "no milk", milk disappearance as indicators of physiological maturity in maix. Milk free stage of the kemel occurred from I to 3 days prior to black layer having developed. The range for harvesting com for silage occurs a kemels mature from 75% milk to no milk. Position of milk line was easy to see. and can be used as a visible indicator to determine com matunty stage\ and whole plant dry matter. Whole plant dry matter increased with advancing maturity. averaged over hybrids it was 24.1, 25.6. 28.5. 34.6 and 39.0% at soft dough, dent, 75% milk. half milk and no milk. Milk line was more usehl indicator in monitoring corn maturity prior to physiological maturity.ing corn maturity prior to physiological maturity.

• Child Health Nursing Research
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• v.13 no.4
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• pp.436-443
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• 2007

Purpose: An exploratory study was done to examine the validity of the new Ballard scale with extended scoring system(eNBS) in estimating gestational age(AG) in full-term newborns. Method: The eNBS scoring system was extended to include all numbers of total score of NBS and GA to allow a 3-days variation in GA estimatio compared to the original scale which has a 2-week variation due to the application of a 5-score interval for the total NBS score and only even numbers for GA. GA by eNBS(GA-eNBS) was compared with GA by LMP(GA-LMP) and GA by standard NBS(GA-sNBS) in 133 full-term newborns. Difference between GA-LMP and GA-eNBS was analyzed for each GA. Results: Positive correlations were observed in GA-sNBS and GA-eNBS with GA-LMP. There was no difference between GA-LMP and GA-eNBS at 39GA and 40GA. At 37GA and 38GA, GA-eNBS overestimated GA-LMP up to 1 week, while underestimating up to 1 week at 41GA. Conclusions: The accuracy of eNBS was validated within 3 days of variation in GA estimation at 39-40GA. Overestimation by eNBS suggests the possible acceleration of fetal maturity in premature newborns, while underestimation, of the deceleration of fetal maturity in postterm newborns.

• Journal of Biosystems Engineering
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• v.30 no.1 s.108
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• pp.38-44
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• 2005

In this study, the acoustic response technique was applied to evaluate the maturity of melon nondestructively. The acoustic response signals through melon were obtained by microphone and signal conditioner with the lapse of days after fruit set. The acoustic parameters such as resonant frequencies and the spectrum energy ratio were analyzed. To investigate the relation between acoustic parameters and firmness of melon, the compression test was performed. Three resonant frequencies representing f1, f2 and f3 were 150 to 250 Hz, 300 to 400 Hz, and 450 to 550 Hz, respectively. The resonant frequencies were shifted to the lower frequencies and the magnitude of spectrum decreased as the maturity of melon increased. Some significant correlations were found between melon firmness and the spectrum energy ratio in some frequency ranges. It is possible to estimate the maturity of melon by acoustic response technique.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.3
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• pp.302-307
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• 1996

Seed coat anthocyanin can be purified by soaking 3 times in methanol solution supplemented with one percent of HCl. Anthocyanin content was very wide range in collected lines and average anthocyanin content of black seed coat lines was 15.07 permillage, but that of white mottled on brown seed coat lines was 0.31 permillage. In black seed coat lines green seed embryo type has more anthocyanin in amount compare to yellow seed embryo. Anthocyanin accumulation was promoted in late maturing lines compare to early maturing lines. Positive correlations were observed among 100 seed weight, days to flowering, days to growing and anthocyanin content, but negative correlation between days from flowering to maturity and anthocyanin content. Collected black seed coat lines were divided into two maturity groups. Group VI which has longer than group V in days to maturity accumulated more anthocyanin compare to group V. When the seeding date was May 15, highest anthocyanin content was observed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.4
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• pp.327-337
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• 1996

A field experiment was conducted at SNU Experimental Livestock Farm, Suweon in 1995 to determine effect of planting date on forage performance of wm hybrids of four different maturity groups. A split-plot design replicated three times was used, with com hybrids representing four maturity groups (115, 118, 121 and 125 days) being the main plots and planting dates (3124, 415, 415, 425, 515 and 5/15) the sub-plots. 1. Days to emergence and percent emergence from the March 24 planting were, on the average, 36 days and 58%, respectively, but those from the April 5 to May 15 planting averaged 12 days and 92%, respectively. 2. Plant and ear heights increased gradually as the dates of planting were delayed except the May 15 planting, however, percent ear was decreased as the dates of planting were delayed. There was a trend for the mean lodging percentage of the hybrids to be higher as the planting date was delayed. 3. The 115-and 118-day mediumearly maturing hybrids harvested on August 18 produced silages with a dry matter content between 27 and 30% at all planting dates except the May 15 planting, while the 121-and 125-day medium-late maturing hybrids produced silages with a dry matter wntent less than 27% regardless of any planting dates. 4. There were no significant differences in mean dry matter yield among the hybrids, but significant mean TDN yield differences were found. The 115-, 118- and 125-day hybrids had significantly higher mean TDN yield than the 121-day hybrid. There were significant differences in mean dry matter and TDN yields among the planting dates. The mean dry matter and TDN yields from the April 5, 15 and 25 plantings were significantly higher than those of other plantings, however, there were no significant differences in mean TDN yield among the April 5, April 15 and April 25 plantings. No significant planting date $\times$ maturity interactions were found for both the dry matter and TDN yields. 5. Mean stover NDF and ADF contents of the 115- and 118day hybrids were higher than those of the 121- and 125-day hybrids, but the reverse was true for mean stover IVDMD and RFV. Mean stover NDF an ADF contents increased with earlier plantings, but mean stover IVDMD and RFV increased when planting was delayed. Results of this experiment indicate that for corn planting in central and northern areas of Korea, early to mid-April may be the right time with the 115-to 118-day maturity hybrids when silage making before August 20 is taken into consideration.

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.6
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• pp.623-633
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• 2017

Food waste has been recognized as a organic sources for composting and many research was conducted to efficiently utilize or treat. This study was to evaluate a feasibility for producing food waste compost under co-composting with mixture of food and animal waste. The mixing ratio of food and animal waste was 35% as main material, which additionally mixed 30% of sawdust for co-composting. Total days of composting experiment were 84 days and each sub samples were collected at every 7 days from starting of composting. Results showed that inner temperature in composting was rapidly increased to $70{\pm}4^{\circ}C$ within 3~5 days depending on mixing animal waste of cattle, pig, and chicken base compared to sole food waste base. Expecially, the CN ratio in the mixture of food and pig water was the highest (16.2) among compost. After finishing composting experiment, maturity was evaluated with solvita and germination test. Maturity index (MI) of the mixture of food and animal waste was ranged between 6~7, but was 3 in sole food waste. Calculated germination index (GI) was at the range of about 100 irrespectively of mixing of food and animal waste. However, NaCl content and heavy metal as Cr, Cu, Ni, Pb, and Zn contents was increased in the mixture of food and animal waste. which was the highest in compost mixed the food and pig waste. Both MI and GI showed that manufactured fertilizer was suitable for fertilizer criteria while sole food waste was not adequate for composting due to composting periods. Overall, mixing the food and animal waste can be utilized for improving compost maturity, but more research should be conducted to make high quality of food waste compost with animal waste in agricultural fields.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.6
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• pp.760-767
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• 2016

Food waste (FW) has been recognized as a critical problem in Korea and many research was conducted to efficiently utilize or treat FW. Main purpose of this research was to evaluate a feasibility for producing fermented organic fertilizer with vegetable waste (VW). Three different organic materials (saw dust, coco peat, and waste mushroom media) were mixed with VW at the rate of 30, 40, 50% respectively. Total days of composting experiment were 35 days and each sub samples were collected at every 5 days from starting of composting. Result showed that inner temperature of composting was increased to $60{\pm}4^{\circ}C$ within 5~10 days depending on varied organic materials and mixing ratio. Among different treatment, the highest increase of inner temperature was observed when 30% of saw dust was mixed with VW. After finishing composting experiment, maturity of each compost was evaluated with solvita and germination test. Maturity index (MI) of each treatment was ranged between 5~7 indicating that manufactured fertilizer was curing or finished stage. Calculated germination index (GI) was at the range of 57.83~101.16 depending on organic materials and mixing ratio. Both MI and GI showed that manufactured fertilizer was met for fertilizer criteria while control (VW only) was not adequate for composting. Overall, VW can be utilized for making organic fertilizer mixing with saw dust, coco peat and more research should be conducted to make high quality of organic fertilizer with vegetable waste.