• 생명과학회지
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• 제20권3호
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• pp.411-415
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• 2010

본 연구에서는 Anisakis 연구를 위하여 웹을 기반으로 하는 데이터베이스를 리녹스 Cent OS 시스템이 설치된 Xeon 3.2 GHz cpu의 인텔 서버플랫폼 ZSS130 (삼성) 서버에 구축하였다. 운영체제를 설치한 후에 common gate interface(cgi) 기반의 웹서버 (http://www.anisakis.org)를 구축하고 NCBI에서 제공하는 WebBLAST 프로그램을 설치하였다. Anisakis 연구를 위한 웹기반 데이터베이스를 다음과 같은 순서로 구축하였다. 우선 회충목에 속하는 각종 서열(염기서열/ 아미노산서열, EST 서열, 미토콘드리아 Genome 서열)들을 멀티파스타 형식으로 다운로드 하였다. 다음으로NCBI에서 제공하는 formatdb 프로그램을 통하여 BLAST 검색이 가능하도록 데이터베이스화 하였으며 모든 염기서열들과 EST 서열들을 TGICL 프로그램을 통하여 clustering 및 assembing을 하였다. 그리고 NLS (Nuclear Localization Signal) 예측을 위해 EST 서열들은 Genscan 프로그램과 Emboss sixpack 프로그램을 사용하여 아미노산으로 변환하였다. 또한 벡터 서열과 E. coli 서열, 그리고 반복 서열들을 서버에 구축하여 서열들의 오염을 확인할 수 있게 하였다. 본 웹데이터베이스 서버의 구축을 통해 고래회충 및 회충목의 염기서열과 일치하는 서열을 자체 BLAST를 통해 매우 빠른 속도로 추출 할 수 있었으며, cDNA나 genomic DNA 라이브러리를 구축할 때 라이브러리의 상태를 쉽게 확인 할 수 있게 되었다. 또한 Clustering Res. 인터페이스를 통해 SNPs 연구 수행 시 매우 쉽게 실험용 시발체를 제작할 수 있으며 기 구축된 cDNA library의 활용을 annotated EST를 통해 극대화 시킬 수 있어 고래회충 관련 분자생물학적 연구에 도움이 될 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• 제19권4호
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• pp.351-357
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• 2009

Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB-1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class III stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-l, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic differences between the antagonism of KB-1111 and KB-1122.

• 한국의류산업학회지
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• 제16권6호
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• pp.971-978
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• 2014

This study investigates the developmental conditions of functional clothes for the elderly. First, preceding studies and relevant web sites were analyzed along with a survey of commercial functional elderly clothes in Korea. A KIPRIS patent information database was used to study registered and disclosed patents and utility models in order to analyze patent application trends in relevant fields. Patents were searched by year of application from 1990 to 2013. Keywords used for searching included 'senior, aged, and elderly'. Among collected data, overlapping and irrelevant data were excluded to select 162 cases for analysis. Details of analysis are annual patent application trends of functional elderly clothes, topic analysis, shape and characteristics. An examination of commercially available functional clothing products for the elderly indicated that most of products were developed in the form of inner wear or protective clothing for seniors who have bodily discomfort. An annual patent application of functional elderly clothes showed slight fluctuations; however, there was an overall increase. For patent topics, technologies related to secretion and excretion were the most at 47 cases total (29%), followed by 23 cases (14%) on biometric information technology. However, the development of relevant technologies seems necessary in the future as therapeutic function and fiber technologies gradually stagnate.

• Journal of Acupuncture Research
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• 제19권3호
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• pp.138-155
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• 2002

Objective : The purpose of this study is to work on the problems and on the direction of the study regarding the evidence-based study of Acupuncture. Methods : We reviewed the PubMed electronic database. Eight articles of systematic review without meta-analysis and six articles without meta-analysis were selected. Results : In the study of eight articles of systematic review without meta-analysis, there were two articles on effectiveness of acupuncture and one on non-effectiveness of acupuncture. Four articles were about the changes according to the quality of randomized controlled trials. Also, one article was about the side-effect of acupuncture. In the study of six articles of systematic review with meta-analysis, there were two articles on Odds Ratio, two on Relative Risk, one on number-needed-to-treat, and two on 18 predefined methodological criteria. Conclusion : As the quality of RCTs was high, positive response decreased and as the quality of RCTs was low, positive response increased. For the study of evidence-based acupuncture, it is necessary that we conduct research on well-planned placebo acupuncture, large sample size, statistical method, method of searching and assessing literature and method of meta-analysis.

• 한국컴퓨터정보학회지
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• 제8권2호
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• pp.33-42
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• 2001

본 논문에서는 웹과 XML을 이용하여 학습자가 원하는 문항만을 검색하고 평가할 수 있는 문제은행 시스템을 구현하였다. 문항의 구성은 4지선다형, 5지선다형, 단답형으로 구성하였고 난이도 및 출제빈도를 고려하여 변별력을 가지도록 문제은행화 하였다. 문항검색은 데이터베이스에 저장된 정보를 단순히 검색하는 것이 아니라 저장된 정보를 XML문서로 변환한 후 XML 데이터에 대하여 Xpath를 이용하여 검색하였다 검색된 결과는 XSL을 이용하여 브라우저상에 나타나도록 하였다. 문항평가는 학습자가 원하는 과목. 단원에 대해서 난이도 및 출제빈도 그리고 문항유형별로 문항수를 입력하여 평가 문항을 생성하였다. 또한 실시간으로 학습자에게 학습결과를 제공하였고 학습자가 오답으로 응답한 문항에 대해서는 반복학습을 할 수 있도록 하였다.

• 보건행정학회지
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• 제27권1호
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• pp.18-29
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• 2017

Background: In Korea, the problem of physician workforce imbalances has been a debated issue for a long time. This study aimed to draw key lessons and policy implications to Korea by analyzing projection models of physician demand/supply among five countries. Methods: We adopted theoretical framework and analyzed detail indicators used in projection models of demand/supply comparatively among countries. A systematic literature search was conducted using PubMed and Google Scholar with key search terms and it was complimented with hand searching of grey literature in Korean or English. Results: As a results, Korea has been used a supply-based traditional approach without taking various variables or environmental factors influencing on demand/supply into consideration. The projection models of USA and Netherlands which considered the diversity of variables and political issues is the most closest integrated approach. Based on the consensus of stakeholder, the evolved integrated forecasting approach which best suits our nation is needed to minimize a wasteful debate related to physician demand/supply. Also it is necessary to establish the national level statistics indices and database about physician workforce. In addition, physician workforce planning will be discussed periodically. Conclusion: We expect that this study will pave the way to seek reasonable and developmental strategies of physician workforce planning.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.163-167
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• 2002

Recently, owing to performance elevation of telecommunication technology, increase of wireless internet's subscriber and diffusion of wireless device, Interest about LBS (Location Based Service) which take advantage of user's location information and can receive information in concerning with user's location is increasing rapidly. So, MOMS (Moving Object Management System) that manage user's location information is required compulsorily to provide location base service. LBS of childhood such as service to find a friend need only current location, but to provide high-quality service in connection with Data Mining, CRM, We must be able to manage location information of past. In this paper, we design distributed manage system to insert and search Moving Object in a large amount. It has been consisted of CLIM (Current Location Information Manager), PLIM (Past-Location Information Manager) and BLIM (Distributed Location Information Manager). CLIM and PLIM prove performance of searching data by using spatiotemporal-index. DLIM distribute an enormous amount of location data to various database. Thus it keeps load-balance, regulates overload and manage a huge number of location information efficiently.

• 한국멀티미디어학회논문지
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• 제2권1호
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• pp.9-21
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• 1999

이 논문에서는 시간지원 데이터의 시간적 특성을 고려해서 과거 세그먼트, 현재 세그먼트, 그리고 미래 세그먼트로 분리해서 저장하는 과거 현재 미래 저장 구조를 제안하였으며, 과거 현재 미래 저장 구조를 기반으로 하는 데이터 이동 기법 두 가지를 제안하였다. 이 논문에서 제안한 과거 현재-미래 저장 구조에서는 데이터 이동 프로세서가 데이터를 이동하기 위한 시기를 결정하는 방법을 제안하였다. 이 논문에서 제안한 데이터 이동 기법 두 가지 중에서, 시간단위에 의한 이동 기법에서는 각 세그먼트에 저장되는 개체 버전을 정의하였고 데이터의 유효성을 검사하고 이동하는 과정을 보였다. 그리고 LST-GET에 의한 이동 기법에서는LST와GET를 정의하였고 LST와GET를 기준으로 각 세그먼트에 저장되는 개체 버전을 정의하였다. 모의 실험을통해서 기존의 저장 구조와 과거-현재 미래 저장 구조에 대해서 사용자 질의에 대한 성능을 비교하였고, 두 가지 데이터 이동 기법의 성능을 분석하였다.

• Journal of Electrical Engineering and Technology
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• 제8권5호
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• pp.1188-1193
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• 2013

In content based image retrieval (CBIR) system, the images are represented based upon its feature such as color, texture, shape, and spatial relationship etc. In this paper, we propose a MRI Image Retrieval using wavelet transform with mahalanobis distance measurement. Wavelet transformation can also be easily extended to 2-D (image) or 3-D (volume) data by successively applying 1-D transformation on different dimensions. The proposed algorithm has tested using wavelet transform and performance analysis have done with HH and $H^*$ elimination methods. The retrieval image is the relevance between a query image and any database image, the relevance similarity is ranked according to the closest similar measures computed by the mahalanobis distance measurement. An adaptive similarity synthesis approach based on a linear combination of individual feature level similarities are analyzed and presented in this paper. The feature weights are calculated by considering both the precision and recall rate of the top retrieved relevant images as predicted by our enhanced technique. Hence, to produce effective results the weights are dynamically updated for robust searching process. The experimental results show that the proposed algorithm is easily identifies target object and reduces the influence of background in the image and thus improves the performance of MRI image retrieval.

• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.1990-1994
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• 2006

Fusarium proliferatum KGL0401 was previously isolated from Physalis alkekengi var. francheti plant roots and exhibited a high GA productivity. A gas chromatography-mass spectrometry (GC-MS) analysis of extracts of the culture fluid of F proliferatum KGL0401 also revealed the presence of $GA_1$, $GA_3$, $GA_4$, $GA_7$, $GA_{20}$, and $GA_{24}$. Therefore, the present study conducted a proteome analysis of waito-c rice treated with the culture fluid of the isolated F proliferatum KGL0401 to identify the protein expression triggered by the GA-containing culture fluid. The results revealed the overexpression of 180 protein spots in the sample treated with the culture fluid. Among them, 75 induced proteins were selected and analyzed by MALDI-TOF (matrix-assisted laser desorption-iorrization time-of-flight) mass spectrometry, followed by database searching, and 51 proteins were identified.