• Title/Summary/Keyword: damping-off

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Biological Control of Pythium Damping-off of Bush Okra Using Rhizosphere Strains of Pseudomonas fluorescens

  • Abdelzaher, Hani M.A.;Imam, M.M.;Shoulkamy, M.A.;Gherbawy, Y.M.A.
    • Mycobiology
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    • v.32 no.3
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    • pp.139-147
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    • 2004
  • A severe damping-off disease of bush okra caused by Pythium aphanidermatum, was diagnosed in plastic houses in Der Attia village, 15 km southwest of El-Minia city, Egypt, during the winter of 2001. Bush okra seedlings showed low emergence with bare patches inside the plastic houses. Seedlings that escaped pre-emergence damping-off showed poor growth, stunting and eventually collapsed. Examination of the infected tissues confirmed only Pythium aphanidermatum, showing its typical intercalary antheridia, and lobulate zoosporangia. P. aphanidermatum was shown to be pathogenic on bush okra under pot and field experiments. Bacteria making inhibition zones against the damping-off fungus P. aphanidermatum were selected. Agar discs from rhizosphere soil of bush okra containing colonies were transferred onto agar plate culture of P. aphanidermatum. After 2 days of incubation, colonies producing clear zones of non-Pythium growth were readily detected. The two bacteria with the largest inhibition zones were identified as Pseudomonas fluorescens. Bush okra emergence(%) in both pot and plastic houses experiments indicated that disease control could be obtained by applying P. fluorescens to the soil or coating the bacteria to the bush okra seeds before sowing. In the plastic houses, application of the bacteria onto Pythium-infested soil and sowing bush okra seeds dressed with bacteria gave 100% emergence. In addition, This was the first reported disease of bush okra by this oomycete in Egypt.

Control of Ginseng Damping-Off Disease Using Chitinolytic Bacterial Mixtures (키틴분해미생물을 이용한 인삼 잘록병 방제)

  • Kim, Young Cheol;Chung, Hyun Chae;Bae, Yeoung Seuk;Park, Seur Kee
    • Research in Plant Disease
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    • v.24 no.4
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    • pp.353-358
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    • 2018
  • An effective bioformulation of mixtures of chitin-degrading bacteria has been used successfully to control plant diseases and nematodes. In this study, the bioformulation approach was assessed to control damping-off disease of ginseng. In pot experiments with soils infested with dapming-off pathogens of ginseng, root-drenchings of Chrobacterium sp. C-61, Lysobacterium enzymogenes C-3, and mixture of two bacterial strains grown in chitin minimal medium were signficantly increased emergence of seeds and reduced damping-off disease incidence of seedlings. Efficacy of the bioformulated product depended on the dose and timing of application. In two-year-old ginseng field, the high control efficacies were achieved by soil drenching of two times with an undiluted product or three times with a 10-fold diluted product. In a To-jik nursery (self soil nursery), biocontrol efficacy of the undiluted product against damping-off disease were similar to that of a seed dressing with fungicide, Tolclofos-methyl WP. These results suggest that the bioformulated product containing Chromobacterium sp. C-61 and L. enzymogenes C-3 could be an effective approach to control of ginseng damping-off disease.

STUDY ON RIDE QUALITY OF A HEAVY-DUTY OFF-ROAD VEHICLE WITH A NONLINEAR HYDROPNEUMATIC SPRING

  • SUN T.;YU F.
    • International Journal of Automotive Technology
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    • v.6 no.5
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    • pp.483-489
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    • 2005
  • Based on a two-degree of freedom vehicle model, this paper investigates ride comfort for a heavy off-road vehicle mounted a nonlinear hydropneumatic spring, which is influenced by nonlinear stiffness and damping characteristics of the hydropneumatic spring. Especially, the damping force is derived by applying H. Blasius formula in modeling process according to the real physical structure of the hydropneumatic spring, and the established model of nonlinear stiffness characteristics have been validated by experiments. Furthermore, the effects of parameter variations of the hydropneumatic spring, such as initial charge pressure and damping coefficient, on body acceleration, suspension deflection and dynamic tire deflection are also investigated.

ASYMPTOTIC STABILIZATION FOR A DISPERSIVE-DISSIPATIVE EQUATION WITH TIME-DEPENDENT DAMPING TERMS

  • Yi, Su-Cheol
    • Journal of the Chungcheong Mathematical Society
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    • v.33 no.4
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    • pp.445-468
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    • 2020
  • A long-time behavior of global solutions for a dispersive-dissipative equation with time-dependent damping terms is investigated under null Dirichlet boundary condition. By virtue of an appropriate new Lyapunov function and the Lojasiewicz-Simon inequality, we show that any global bounded solution converges to a steady state and get the rate of convergence as well, when damping coefficients are integrally positive and positive-negative, respectively. Moreover, under the assumptions on on-off or sign-changing damping, we derive an asymptotic stability of solutions.

The Output Characteristics Analysis by Cut-off Frequency Set-up of the LCR Filter on NPC Multi-Level Inverter with Trap-Filter (트랩필터를 갖는 NPC멀티레벨 인버터의 LCR필터 차단주파수 설정에 따른 출력특성 분석)

  • Kim, Soo-Hong;Kim, Yoon-Ho
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.56 no.5
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    • pp.892-897
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    • 2007
  • This paper presents the output filter design and the output characteristic analysis by cut-off frequency set up of the LCR filter on NPC multi-level inverter with trap-filter. The single-phase NPC three-level inverter operates at low switching frequency. The proposed LC trap filter is comprised of a conventional LCR output filter, by using LC trap filter the need for high damping resistor and low LC cut-off frequency is eliminated. Also. low damping resistor is increased the output filter system. The multilevel inverter system used NPC type inverter in proper system for high power application and controller is used DSP(TMS320C31). The effectiveness of proposed system confirmed the validity through SPICE simulation and experimental results.

Control of Crisphead Lettuce Damping-off and Bottom Rot by Seed Coating with Alginate and Pseudomonas aeruginosa LY-11

  • Heo, Kwang-Ryool;Lee, Kwang-Youll;Lee, Sang-Hyun;Jung, Soon-Je;Lee, Seon-Woo;Moon, Byung-Ju
    • The Plant Pathology Journal
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    • v.24 no.1
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    • pp.67-73
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    • 2008
  • Seedling damping-off and bottom rot caused by Rhizoctonia solani are yield limiting diseases of crisphead lettuce. To provide biocontrol measure in the management of the diseases, biocontrol strain Pseudomonas aeruginosa LY-11 was isolated from lettuce rhizosphere and introduced into crisphead lettuce rhizosphere by the seed coating delivery method. Alginate was used as a coating material to generate beads containing $10^6-10^{6.5}$ colony-forming units (CFUs) of viable bacterial cells of LY-11. When seeds germinated from the alginate beads containing the strain LY-11, the bacteria established mostly in plant rhizosphere to maintain at least $10^4$ CFU per gram of plant tissues. Crisphead lettuce seedlings germinated from the entrapped seeds were less affected from damping-off and bottom rot with disease control values of 70.4% and 85.4% respectively. Although P. aeruginosa LY-11 colonized plant rhizosphere and not phyllosphere, the result indicated that bottom rot caused by the foliar inoculation of R. solani was effectively reduced by the rhizobacteria. All data suggested that immobilized rhizobacterial application in seeds by alginate coating could control damping-off and induce induced systemic resistance of crisphead lettuce to reduce bottom rot.

Fungicide Screening for Control of Summer Spinach Damping-off Caused by Rhizoctonia solani (Rhizoctonia solani에 의한 여름 시금치 잘록병의 방제를 위한 살균제 선발)

  • Kim, Byung-Sup;Yun, Yue-Sun;Yun, Choel-Soo;Zhang, Xuan-Zhe;Yeoung, Young-Rog;Hong, Sae-Jin
    • The Korean Journal of Pesticide Science
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    • v.9 no.1
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    • pp.35-40
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    • 2005
  • Damping-off of summer spinach caused by Rhizoctonia solani AG-4 has become a very important disease. For the control of summer spinach damping-off, antifungal activity of thirteen fungicides (pencycuron, trifloxystrobin, pyraclostrobin, azoxystrobin, kresoxim-methyl, validamycin, fluazinam, Benlate-T, flutolanil, cyazofamid, hexaconazole, tebuconazole, prochloraz) were evaluated in vitro and in vivo. Pencycuron, pyraclostrobin, validamycin, fluazinam, Benlate-T, hexaconazole, tebuconazole, and flutolanil significantly suppressed the mycelial growth of the pathogenic fungus. However, trifloxystrobin, azoxystrobia kresoxim-methyl, cyazofamid, and prochloraz did not represent good inhibition on the growth of R. solani. When applied by soil drenching (2,000 mg/L), pencycuron, pyraclostrobin, validamycin, fluazinam, Benlate-T, and flutolanil provided spinach survival ratios of 97.8%, 84.4%, 93.3%, 95.6%, 91.1%, and 86.7%, respectively. Also when treated in seed at 2,000 ing/L, pencycuron and pyraclostrobin displayed survival ratios of more than 85.1%.

Biocontrol of Ginseng Damping-off by Bacillus velezensis CC112 (Bacillus velezensis CC112 균주의 인삼 잘록병에 대한 생물적 방제)

  • Lee, Sang Yeob;Song, Jaekyeong;Park, Kyeong Hun;Weon, Hang Yeon;Kim, Jeong Jun;Han, Ji Hee
    • The Korean Journal of Mycology
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    • v.44 no.3
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    • pp.176-183
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    • 2016
  • Bacillus velezensis CC112 inhibited the mycelial growth of several plant pathogens, including Rhizoctonia solani, causing damping-off on ginseng. The control efficacies of B. velezensis CC112 against R. solani by seed dipping in LB and BSM broth diluted 10 times, soil dipping, and soil drenching with LB broth diluted 10 times were 65.8%, 67.1%, and 64.2%, respectively. Treatment of soil drenching with the 100 times diluted prototype of B. velezensis CC112 against R. solani and Pythium sp. by soil revealed control efficacies of 77.3% and 65.7%, respectively. These results indicate that B. velezensis CC112 is a prospective biofungicide for the biological control of ginseng damping off.

First Report of Fusarium oxysporum Causing Damping-off on Paprika in Korea (Fusarium oxysporum에 의한 파프리카 잘록병)

  • Park, Mi-Jeong;Back, Chang-Gi;Seo, Yunhee;Park, Jong-Han
    • Research in Plant Disease
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    • v.25 no.2
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    • pp.94-97
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    • 2019
  • In February 2019, a damping-off disease occurred at the seedling stage of paprika in a commercial nursery located in Cheorwon, Korea. A species of Fusarium was isolated from the diseased plant and it was identified as Fusarium oxysporum based on morphological characteristics and nucleotide sequence data of translation elongation factor $1-{\alpha}$ and the largest subunit of RNA polymerase. The isolate obtained was revealed to be pathogenic to the host plant through pathogenicity tests, and the reisolation of the pathogen confirmed Koch's postulates. This is the first report of damping-off caused by Fusarium oxysporum on paprika in Korea.