• The Korean Journal of Physiology and Pharmacology
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• v.1 no.2
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• pp.209-219
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• 1997

Previous studies have demonstrated that oxidative stress involving generation of reactive oxygen species (ROS) is responsible for the cytotoxic action of $TNF{\alpha}$. Protective effect of small heat shock proteins (small HSP) against diverse oxidative stress conditions has been suggeted. Although overexpression of small hsp was shown to provide an enhanced survival of $TNF{\alpha}$-sensitive cells when challenged with $TNF{\alpha}$, neither the nature of $TNF{\alpha}$-induced cytotoxicity nor the protective mechanism of small HSP has not been completely understood. In this study, we have attempted to determine whether $TNF{\alpha}$ induces oxidative DNA damage in $TNF{\alpha}$-sensitive L929 cells. We chose to measure the level of 8-hydroxy-2'-deoxyguanosine (8 ohdG), which has been increasingly recognized as one of the most sensitive markers of oxidative DNA damage. Our results clearly demonstrated that the level of 8 ohdG increased in L929 cells in a $TNF{\alpha}$ dose-dependent manner. Subsequently, we asked whether small HSP has a protective effect on $TNF{\alpha}$-induced oxidative DNA damage. To accomplish this goal, we have stably transfected L929 cells with mouse small hsp cDNA (hsp25) since these cells are devoid of endogenous small hsps. We found that $TNF{\alpha}$-induced 8 ohdG was decreased in cells overexpressing exogenous small hsp. We also found that the cell killing activity of $TNF{\alpha}$ was decreased in these cells as measured by clonogenic survival. Taken together, results from the current study show that cytotoxic mechanism of $TNF{\alpha}$ involves oxidative damage of DNA and that overexpression of the small hsp reduces this oxidative damage. We suggest that the reduction of oxidative DNA damage is one of the most important protective mechanisms of small HSP against $TNF{\alpha}$.

• BMB Reports
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• v.34 no.6
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• pp.489-495
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• 2001

DNA damage by UV and environmental agents are the major cause of genomic instability that needs to be repaired, otherwise it give rise to cancer. Accordingly, mammalian cells operate several DNA repair pathways that are not only responsible for identifying various types of DNA damage but also involved in removing DNA damage. In mammals, nucleotide excision repair (NER) machinery is responsible for most, if not all, of the bulky adducts caused by UV and chemical agents. Although most of the proteins involved in NER pathway have been identified, only recently have we begun to gain some insight into the mechanism by which proteins recognize damaged DNA. Binding of Xeroderma pigmentosum group C protein (XPC)-hHR23B complex to damaged DNA is the initial damage recognition step in NER, which leads to the recruitment of XPA and RPA to form a damage recognition complex. Formation of damage recognition complex not only stabilizes low affinity binding of XPA to the damaged DNA, but also induces structural distortion, both of which are likely necessary for the recruitment of TFIIH and two structure-specific endonucleases for dual incision.

• Structural Engineering and Mechanics
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• v.33 no.6
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• pp.657-674
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• 2009

In this paper a 3-D continuum damage mechanics formulation for composite laminates and its implementation into a finite element model that is based on the layer-wise laminate plate theory are described. In the damage formulation, each composite ply is treated as a homogeneous orthotropic material exhibiting orthotropic damage in the form of distributed microscopic cracks that are normal to the three principal material directions. The progressive damage of different angle ply composite laminates under quasi-static loading that exhibit the free edge effects are investigated. The effects of various numerical modeling parameters on the progressive damage response are investigated. It will be shown that the dominant damage mechanism in the lay-ups of [+30/-30]s and [+45/-45]s is matrix cracking. However, the lay-up of [+15/-15] may be delaminated in the vicinity of the edges and at $+{\theta}/-{\theta}$ layers interfaces.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.09c
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• pp.86-95
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• 2010

Ground movements during tunnelling have the potential for major impact on nearby buildings, utilities and streets. The impacts on buildings are assessed by linking the magnitude of ground loss at the source of ground loss around tunnel to the lateral and vertical displacements on the ground surface, and then to the lateral strain and angular distortion, and resulting damage in the building. To prevent or mitigate the impacts on nearby buildings, it is important to understand the whole mechanism from tunnelling to building damage. This paper discusses tunneling-induced ground movements and their impacts on nearby buildings, including the importance of the soil-structure interactions. In addition, a building damage criterion, which is based on the state of strain, is presented and discussed in detail and the overall damage assessment procedure is provided for the estimation of tunnelling-induced building damage considering the effect of soil-structure interaction.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.13 no.4
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• pp.106-112
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• 2004

In particle or short-fiber reinforced composites, cracking or debonding of the reinforcements cause a significant damage mode because the damaged reinforcements lose load carrying capacity. The average stress in the inhomogeneity represents its load carrying capacity, and the difference between the average stresses of the intact and broken inhomogeneities indicates the loss of load carrying capacity due to cracking damage. The composite in damage process contains intact and broken reinforcements in a matrix. An incremental constitutive relation of discontinuously-reinforced composites including the progressive cracking damage of the reinforcements have been developed based on the Eshelby's equivalent inclusion method and Mori-Tanaka's mean field concept. Influence of the cracking damage on the stress-strain response of the composites is demonstrated.

• Wind and Structures
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• v.13 no.2
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• pp.191-206
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• 2010

Windborne debris is a major cause of structural damage during severe windstorms and hurricanes owing to its direct impact on building envelopes as well as to the 'chain reaction' failure mechanism it induces by interacting with wind pressure damage. Estimation of debris risk is an important component in evaluating wind damage risk to residential developments. A debris risk model developed by the authors enables one to analytically aggregate damage threats to a building from different types of debris originating from neighboring buildings. This model is extended herein to a general debris risk analysis methodology that is then incorporated into a vulnerability model accounting for the temporal evolution of the interaction between pressure damage and debris damage during storm passage. The current paper (Part I) introduces the debris risk analysis methodology, establishing the mathematical modeling framework. Stochastic models are proposed to estimate the probability distributions of debris trajectory parameters used in the method. It is shown that model statistics can be estimated from available information from wind-tunnel experiments and post-damage surveys. The incorporation of the methodology into vulnerability modeling is described in Part II.

• Journal of Ocean Engineering and Technology
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• v.12 no.3 s.29
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• pp.49-59
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• 1998

The damage mechanism of $Cr_2O_3$ plasma coated soda-lime glass and uncoated glass by steel ball particle impact was analyzed in this study. And the shape variation of the cracks was investigated by stereo-microscope according to the impact velocity and steel ball diameter. In order to improve the damage reduction effect by $Cr_2O_3$ coating layer, crack size was measured and surface erosion state was observed for both of two kinds of specimen after impact experiment. And the results were compared with each other. The 4-point bending test was performed according to ASTM D790 testing method to evaluate the effect of coating layer for bending strength variation. As a result, it was found that the crack size of $Cr_2O_3$ coated specimen was smaller than that of uncoated one, because of the impact absorption by interior pores in the coating layer and the load dispersion by the structural characteristic of the coating layer. For the specimens subjected to the steel ball impact, the bending strength of coated specimen was higher than that of uncoated specimen.

• Current Optics and Photonics
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• v.3 no.6
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• pp.502-509
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• 2019

Based on the typical pixel structure and parameters of a polysilicon uncooled bolometer, the absorption rate of a polysilicon microbridge infrared detector for 10.6 ㎛ laser energy was calculated through the optical admittance method, and the thermal coupling model of a polysilicon microbridge component irradiated by far infrared laser was established based on theoretical formulas. Then a numerical simulation study was carried out by means of finite element analysis for the actual working environment. It was found that the maximum temperature and maximum stress of the microbridge component are approximately exponentially changing with the laser power of the irradiation respectively and that they increase monotonically. The highest temperature zone of the model is gradually spread by the two corners of the bridge surface that are not connected to the bridge legs, and the maximum stress acts on both sides of the junction of the microbridge legs and the substrate. The mechanism of laser-induced hard damage to polysilicon detectors is the melting damage caused by high temperature. This paper lays the foundation for the subsequent study of the interference mechanism of the laser on working state polysilicon detectors.

• Journal of Advanced Marine Engineering and Technology
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• v.28 no.2
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• pp.332-337
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• 2004

The use of gas absorption refrigeration machine has considerably increased because of the shortage of the electric power in the summer and the regulation of freon refrigerant. Gas absorption refrigeration machine consists of a condenser, a heat exchanger. supplying pipes, a radiator etc, This system is likely to be corroded by acid. dissolved oxygen and gases, Cavitation erosion-corrosion by flow velocity of cooling water may happen in absorption refrigeration machine. In these cases. erosion and corrosion occur simultaneously. Then, it makes a serious damage with synergy effect. Therefore, this paper was studied on the cavitation damage of cupronickel(70/30) tube for gas absorption refrigeration machine, In the $30^{\circ}C$ tap water, linear polarization test and anodic polarization test were carried out for copper(C1220T-OL) and cupronickel(70/30) tube. Also, cavitation erosion-corrosion behavior of cupronickel (70/30) tube was considered, The main results are as following: (1) In the linear test, the corrosion current density of cupronickel(70/30) is higher than that of copper. (2) The erosion-corrosion rate of cupronickel(70/30) displayed later tendency than that of copper by vibratory cavitation in cooling water. (3) In cooling water, the progress mechanism of erosion-corrosion rate of copper and cupronickel(70/30) follows a pattern of incubation, acceleration, attenuation and a steady state period.

• The Korean Journal of Food & Health Convergence
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• v.5 no.6
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• pp.1-4
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• 2019

Wernicke korsakoff syndrome is caused by thiamine deficiency in the body. Thiamine not available in the body, is a substance to be taken from outside with foods. There are some conditions that reduce the metabolism of thiamine taken from the body and cause a vital risk. The most important factor is alcoholism. Wernicke Korsakoff syndrome produces both neurological and vestibular symptoms. At the same time, the damage of these symptoms to the patient psychology cannot be ignored. The aim of this study is to investigate the damage and mechanism of the syndrome in the vestibular system. In this study, we investigated vestibular symptoms of Wernicke Korsakoff syndrome due to thiamine deficiency, differences of vestibular system according to individuals and mechanism of damage caused by syndrome in vestibular system. Thiamine deficiency is caused by Wernicke Karsokoff syndrome with some external factors. This syndrome shows the most important effects of alcoholism. It causes neurological, vestibular and psychological symptoms. In this context, we can say that thiamine deficiency is a disease that causes damage in the vestibular system due to nystagmus formation and imbalance. The most important detail in the treatment stage is the detailed evaluation of symptoms associated with each other.