• Asian-Australasian Journal of Animal Sciences
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• 제33권1호
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• pp.79-90
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• 2020

Objective: In the present study, an liquid chromatography/mass spectrometry (LC/MS) metabolomics approach was performed to investigate potential biomarkers of milk production in high- and low-milk-yield dairy cows and to establish correlations among rumen fluid metabolites. Methods: Sixteen lactating dairy cows with similar parity and days in milk were divided into high-yield (HY) and low-yield (LY) groups based on milk yield. On day 21, rumen fluid metabolites were quantified applying LC/MS. Results: The principal component analysis and orthogonal correction partial least squares discriminant analysis showed significantly separated clusters of the ruminal metabolite profiles of HY and LY groups. Compared with HY group, a total of 24 ruminal metabolites were significantly greater in LY group, such as 3-hydroxyanthranilic acid, carboxylic acids, carboxylic acid derivatives (L-isoleucine, L-valine, L-tyrosine, etc.), diazines (uracil, thymine, cytosine), and palmitic acid, while the concentrations of 30 metabolites were dramatically decreased in LY group compared to HY group, included gentisic acid, caprylic acid, and myristic acid. The metabolite enrichment analysis indicated that protein digestion and absorption, ABC transporters and unsaturated fatty acid biosynthesis were significantly different between the two groups. Correlation analysis between the ruminal microbiome and metabolites revealed that certain typical metabolites were exceedingly associated with definite ruminal bacteria; Firmicutes, Actinobacteria, and Synergistetes phyla were highly correlated with most metabolites. Conclusion: These findings revealed that the ruminal metabolite profiles were significantly different between HY and LY groups, and these results may provide novel insights to evaluate biomarkers for a better feed digestion and may reveal the potential mechanism underlying the difference in milk yield in dairy cows.

• 한국임상수의학회지
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• 제33권6호
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• pp.351-355
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• 2016

This study was conducted to investigate reasons for culling of Chungcheong dairy herds and to investigate the relationships between cow parity, interval from calving to culling, or season and culling reasons. Lactation data from 826 cows on 9 dairy farms from 2013 to 2015, including reasons for culling, dates of birth, last calving, and culling, were analyzed. The overall culling rate was 29.8% (246/826), with mammary problems (23.2%) being the most prevalent reason for culling, followed by infertility (14.2%) and downer cow syndrome (11.0%). The culling rate varied among farms (18.8-43.1%, p < 0.05), but was greater in parity $4{\leq}(40.7%)$ than parities 1, 2, or 3 (17.9-23.6%), whereas the rate was less frequent during mid lactation (21.1%) than in early (36.2%) or late (42.7%) lactation. Moreover, the culling rate was greater during summer (35.8%) than other seasons (20.3-23.2%). Mammary problems, downer cow syndrome, and metabolic disorders were the more common reasons for culling during early lactation, whereas infertility was predominant during late lactation. Our data show that the culling rate in Chungcheong dairy herds was associated with cow parity, interval from calving to culling, and season, with the main reasons for the removal of cows from dairy herds being mammary problems, infertility, and downer cow syndrome.

• 대한수의사회지
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• 제15권8호
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• pp.463-466
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• 1979

In treatment of empyema of frontal sinus in a dairy cow, modified trephing method was applied and aquired satisfactory drainage of pus and discharge from frontal sinus. Methods of trephing opening applied were as follows : 1. Site of trephing opening for

• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.544-545
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• 1989

• Asian-Australasian Journal of Animal Sciences
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• 제26권11호
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• pp.1569-1576
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• 2013

The study was conducted to evaluate the effect of vitamin E and zinc supplementation on energy metabolites, lipid peroxidation, and milk production in peripartum Sahiwal cows. For this, thirty-two pregnant dry Sahiwal cows were selected at sixty days prepartum and divided into four groups viz control, $T_1$, $T_2$, and $T_3$ of eight each. Group $T_1$ were supplemented with zinc at 60 ppm/d/cow, group $T_2$ were supplemented with vitamin E at 1,000 IU/d/cow and group $T_3$ were supplemented with combination of vitamin E at 1,000 IU/d/cow and zinc at 60 ppm/d/cow during d 60 prepartum to d 90 postpartum. Blood samples were collected on d -60, -45, -30, -15, -7, -3, 0, 3, 7, 15, 30, 45, 60, 90, and 120 with respect to day of parturition and analysed for glucose, non esterified fatty acid, and thiobarbituric acid reactive substance. Body condition score was maintained significantly better (p<0.05) in $T_3$ than in the control, $T_1$ and $T_2$ groups. Overall glucose level was higher (p<0.05) in $T_3$ than control, $T_1$, and $T_2$ groups. Levels of nonesterified fatty acid, and thiobarbituric acid reactive substance were lower (p<0.05) in $T_3$ than control, $T_1$, and $T_2$ groups. Milk yield was higher (p<0.05) in $T_3$ than control, $T_1$, and $T_2$ groups. In conclusion, the present study indicated that the supplementation of vitamin E and zinc in peripartum Sahiwal cows enhanced milk production by reducing negative energy balance.

• 한국동물위생학회지
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• 제23권1호
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• pp.61-69
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• 2000

Thirty-one dairy cow from two farm(more than 500,000 cells/ml of bulk milk) in Kyongbuk northern province were selected because of their high somatic cell(more than 500,000 cells/ml of milk In individual cow). Each cow received. staphylococcus aureus vaccine(Labac Staph) and immunostimulant(Ultracon) by intramuscular injection to be repeated every fifteen days for S times. The present study was investigated variation of somatic cell after administration of Labac Staph and Ultracon, and antibiotics resistance of isolated staphylococcus spp from milk in selected cow. The results obtained through the survey were summarized as follows ; 1. Ten dairy cow was injected in A farm. Chronic mastitic two cow after 2rd injection was weeded out the herd. Decrease rate of somatic cell after 1st, 2nd, ,3rd, 4th and 5th administration were 41.4%, 35.6%, 56.4%, 65.4% and 36.7%, respectively. Twenty-one cow was injected in B farm. Chronic mastitic five cow after ,3rd injection was weeded out the herd. Decrease rate of somatic cell after 1st, 2nd, 3rd, 4th and 5th administration were 36.9%, 59.9%, 24.5%, 62.6% and 78.4%, respectively. 2. In A farm, isolated staphylococcus spp were identified as S hyicus 2 strains(11.8%), coagulase negative staphylococcus 15 stains(89.2%) and S epidermidis 6strain(35.3%). In B farm, isolated staphylococcus spp were identified as S aureus 19 strains(55.98%) and coagulase negative staphylococcus 15 strains (44.2%). 3. In A fm, antibiotics resistant rate of isolated staphylococcus spp was high at ampicillin, penicillin and kanamycin, and middle at neomycin, streptomycin and erythromycin. in B farm, antibiotics resistant rate was moderate at ampicillin, penicillin, gentamicin, ka-namycin, neomycin, streptomycin, erythromycin and tetracycline, and coagulase negative staphylococcus spp was moderate at streptomycin.

• 대한수의학회지
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• 제27권1호
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• pp.61-68
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• 1987

This study was conducted to determine the microorganisms inhabitating in cow genitalia and their antimicrobial drug susceptibility. During the period between July, 1985 and February, 1986, a total of 111 cow genitalia, 58 from Korean native and 53 from dairy cow, were sampled at three abattoirs. Gross pathological examination and bacterial isolation and identification were performed from the genital samples. In addition antimicrobial drug susceptibility test for the microorganisms isolated, some synergistic activity among drugs were examined on the major organism isolated from the cases of endometritis and pyometra. The results are summerized as follows: 1. Among the bacteria isolated from the genitalia, Staphylococcus spp., C. pyogenes, E. coli, Proteus spp., Streptococcus spp., Bacillus spp. were most frequently isolated whereas the genera of Pasteurella, Pseudomonas, Klebsiella and Yersina were detected far less frequently. 2. In Korean native cow the genera of Straphylococcus and Steptococcus were more isolated than dairy cow while in dairy cow the genera of Corynebacterium, Proteus, Escherichia were more of ten isolated than Korean native cow. 3. From cow genital organs showing lesions of endometritis and prometra, C. pyogenes was most frequently isolated, the isolation rate being 60 percent, and follow by Staphylococcus spp., Proteus spp., E. coli and Pasteurella spp. in the order. 4. Antimicrobial drug susceptibility test conducted on the major organisms isolated showed that all the isolates were susceptible to gentamicin, cephalosporin and sulfisoxazole, but resistant to tetracycline and penicillin. 5. Twenty-nine isolates of C. pyogenes were submitted to the synergistic activity test of cephalosporin, kanamycin and streptomycin with penicillin. Synergists were demonstrated in 90 percent, 31 percent and 27 percent of isolates examined by the combined use of penicillin and cephalosporin, penicillin and kanamycin, penicillin and streptomycin, respectively. About 10 percent of the isolates were found to be indifferent by the synergism test.

• Journal of Biosystems Engineering
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• 제37권2호
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• pp.122-129
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• 2012

Purpose: Recent mechatronics technology is the most appropriate high technology in agricultural applications to save repetitious labor. Method: Cow's body parameters were measured by several traditional measurers. Image processing technology was used to measure automatically their parameters to reduce labor and time. The parameters were measured form a small model cow which is easily measured, instead to a real cow. The image processing system designed and built for this project was composed of a PC, grabber card, and two cameras, which are located on the side and the top of the model cow. Tests of verification had measured 10 dairy cows. Result: Nine parameters of the model cow's body were measured, and the difference between the real data and the data by image processing was less than 16.7%. Based on the results of the research, the parameters of a real cow had measured of chest depth, withers height, Pelvic arch height, body length, slope body length, chest width, hip width, thurl width, and pin bone width were compared with image processing data. Conclusions: In the Demonstration test, Result had obtained similar data of cow model experiments, and the most of errors were shown less than 5% relatively good result.

• 대한환경공학회지
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• 제37권1호
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• pp.52-59
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• 2015

최근 낙농업은 지구온난화 등 환경에 미치는 영향 문제에 직면하고 있다. 특히 젖소로부터 발생하는 장내발효 메탄가스와 젖소를 사육하는데 필요한 사료작물의 재배에 의한 여러 환경영향이 이슈가 되고 있다. 유럽에서는 Product Environmental Footprint (PEF)라는 통합적 라벨링제도를 통해 공산품뿐만 아니라 낙농제품 등과 같은 각종 농업생산물에 대한 환경영향 산정 방법론을 연구하고 있는 실정이다. 이 연구에서는 전과정평가(Life cycle assessment, LCA)를 이용하여 국내 낙농우(젖소)의 전과정에 대해 전과정 단계별로 환경영향을 산정하고 주요이슈를 규명하였다. 시스템 경계는 젖소를 위한 사료작물 재배, 사료가공, 전소 관리와 가축분뇨처리(cradle-to-gate)이다. 기능단위는 1 kg의 우유(Fat Protein Corrected Milk, FPCM(유지 및 단백질 보정유)) 생산이다. 국내 낙농우(젖소) 시스템의 환경영향은 사육단계, 사료작물 재배단계가 분뇨처리단계 및 사료생산 단계보다 더 많은 환경영향범주에서 주요 전과정 단계로 규명되었다. 이에 농장 내 우유 생산과정 환경영향을 저감하기 위해서는 사육 시 장내발효 메탄가스 발생 억제기술 개발, 농장 기기 장비의 에너지효율 개선, 작물 재배 시 발생하는 침출수 관리 및 사료작물 재배 시 비료의 유실방지 기술 개발 등이 주요 환경이슈를 개선하기 위한 방안으로 판단된다.

• 한국유기농업학회지
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• 제24권4호
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• pp.957-967
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• 2016

The present study was conducted to investigate effect of dietary protected amino acid on milk yield and composition in dairy cow using meta-analysis. Total 21 research papers were employed in analysis, and mixed model was used for the analysis of effects. Effect of protected methionine (PM) and combination of protected methionine and lysine (PML) were investigated under two different levels of dietary crude protein (CP, <18% and >18%). For performance of dairy cow, milk yield, milk composition including milk fat and protein content and yield and 4% FCM (fat corrected milk) production were used for analysis. In case of milk yield, a trend of increment was found at PM supplementation at low CP (P=0.055). However, the effect of PM at high CP was detected as not significant (P>0.05). In case of milk protein, inclusion of PM at low CP showed significant decrement (P<0.05). However, there was no significant effect of MP on milk protein at high CP (P>0.05). Supplementation of MP at high CP level showed significant increment of milk fat (P<0.05). MP supplementation represented significant increment of 4% FCM production (P<0.05) regardless of dietary CP levels. Effects of PML on milk yield and composition at both of low and high dietary CP were not significant in this study. However, it seem to be that there was a possible positive effect of MPL application at high dietary CP on performance of dairy cow.