• 한국식품영양과학회지
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• 제46권6호
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• pp.659-670
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• 2017

산화스트레스와 염증은 간 손상의 진행과정에 중요한 인자로 작용한다. 굴가수분해물의 항산화 및 항염증 활성은 지질대사, 혈압 및 혈당, 면역기능의 조절과 같은 다양한 기능에 관여한다. 그러나 급성 간 손상 모델에서 굴가수분해물의 효과를 확인한 연구 결과는 아직 확인된 바 없다. 본 연구는 LPS/D-GalN에 의해 유도된 급성 간 손상 생쥐 모델에서 굴가수분해물의 효과를 확인하기 위해 수행되었다. 실험군은 대조군(생리식염수), LPS/D-GalN 간 손상군, LPS/D-GalN과 굴가수분해물(100 mg/kg, 200 mg/kg, 400 mg/kg)의 병합투여군 및 LPS/D-GalN과 silymarin(25 mg/kg) 병합투여군으로 나누었다. 급성 간 손상 모델은 $1{\mu}g/kg$의 LPS와 400 mg/kg의 D-GalN으로 유도되었다. 먼저 시료의 항산화 및 항염증 활성을 분석한 결과 굴가수분해물은 농도 의존적으로 높은 DPPH 및 ABTS 라디칼 소거 활성을 보였으며, 인간 정상 간세포주(Chang)에서 과산화수소에 의한 세포 내 활성산소의 생성을 유의적으로 감소시켰다. 또한, 굴가수분해물은 농도 의존적으로 높은 COX-2 및 5-LOX 억제능을 보였으며, LPS에 의해 활성화된 생쥐 대식세포주 RAW264.7에서 발현되는 $TNF-{\alpha}$, IL-6 및 $IL-1{\beta}$의 염증성 사이토카인의 mRNA 발현률을 감소시켰다. 굴가수분해물 투여는 LPS/D-GalN에 의한 혈청 ALT 및 AST 증가를 유의적으로 감소시켰으며, 간 조직의 출혈 및 간세포의 자멸사를 감소시켰다. 또한, 간 균질의 $TNF-{\alpha}$, $IL-1{\beta}$ 및 IL-6 함량을 감소시켰으며, 감소한 catalase의 활성을 유의적으로 증가시켰다. 이상의 결과로부터 굴가수분해물은 간 보호 효과를 가지는 것으로 판단되며, 급성 간 손상의 예방 및 치료에 도움이 될 수 있는 시료로 활용될 수 있을 것으로 기대된다.

• Journal of Nutrition and Health
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• 제33권1호
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• pp.23-32
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• 2000

The purpose of this study was to investigate the effcts of n-3, n-6 fatty arid and d-limonene on histopathological and biochemical changes in experimental rat hepatocarcinogenesis. To attain the above objectives, weanling Sprague-Dawley female rats were intraperitoneally injected twice with a dose of diethylnitrosamine(DEN, 50mg/kg body weight) and after 1 week 0.05% phenobarbital was provided with water. Sardine oil rich in n-3 fatty acids and corn oil rich in n-6 fatty acids were fed at 15% by weight and 5% d-limonene was added to the diet in each group. Ten weeks or 20 weeks after DEN treatment, rats were sacrifirced. The formation of glutathione S-transferase placental form positive(GST-P$\^$+/) foci was significantly decreased by the treatment of either sardine oil or d-limonene HMG-CoA reductase activity was not affected by dietary oils and d-limonene. Protein kinase C (PKC) activity was decreased by either sardine oil or d-limonene. Particularly d-limonene decreased the membrane PKC activity. Membrane Cholesterol/Phospholipid(Chol/PL) ratio was significantly decreased by d-limonene in sardine oil group. The data showed that GST-P$\^$+/ foci number was positively correlated with membrane PKC activity and serum cholesterol and negatively correlated with liver cholesterol level. These results suggest informations about the correlation between histopathological and biochemical changes such as cholesterol metabolism and PKC activity in experimental hepatocarcinogenesis and thereby can elucidate the possible mechanism related to the cancer inhibition.(Korean J Nutrition 33(1) : 23-32, 2000)

• 한국표면공학회지
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• 제39권2호
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• pp.70-75
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• 2006

As technology advances, there is a demand for development of hard, solid lubricant coating. (Ti,Cr)N-$MoS_2$, films were deposited on SKD 11 tool steel substrate by co-deposition of $MoS_2$, with (Ti,Cr)N using a D.C. magnetron sputtering process. The influence of the $N_2/Ar$ gas ratio, the deposition temperature, the amount of $MoS_2$ in the films, and the bias voltage on the mechanical and the structural properties of the films were investigated. Wear tests were performed on the films deposited in various conditions.

• Asian-Australasian Journal of Animal Sciences
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• 제22권5호
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• pp.667-673
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• 2009

Ninety six intact male sheep (12 months old with mean live weight of about 35 kg) were used to assess the effects of restricted feeding on intake, digestion, nitrogen balance and metabolizable energy (ME). The animals were selected from two known Iranian small and large body size breeds: 48 Sangsari (S) and 48 Afshari (A), and were divided into two equal groups: restricted (R) and a control (C). Each group had 48 sheep (24 each breed). The experiment had a duration of 15 and 75 days adaptation and treatment periods, respectively. The animals were individually placed in metabolism cages and fed a diet based on pelleted concentrate mixture consisting of alfalfa, barley grain, cottonseed meal and barley straw. The animals in group C were fed ad libitum, while animals in group R were fed at maintenance level and maintained a relatively constant live weight. During the experiment, the average daily weight gain (ADG) of S and A animals in R group was 0.34 and -0.25 g/d (0.02 and -0.02 $g/kg^{0.75}/d$), respectively. While that of S and A animals in C group was 174.4 and 194.4 g/d (10.16 and 11.48 $g/kg^{0.75}/d$), respectively. Nitrogen (N) was determined by both measured and regression methods. Animals of R group stayed at about zero N balance (0.01 and -0.00 g $N/kg^{0.75}/d$ for S and A animals, respectively). The N retention of animals of both S and A breeds in C group were similar (0.45 and 0.46 g $N/kg^{0.75}/d$, respectively). Digestible organic matter intake (DOMI) and ME requirement for maintenance (MEm) were measured by both constant weight technique and regression method by regressing N balance on DOMI and ME intake on ADG. The measured DOMI during constant weight was 24.61 and 24.27 g $DOMI/kg^{0.75}/d$ and the calculated DOMI from regression equation was 24.24 and 24.22 g $DOMI/kg^{0.75}/d$, for S and A animals, respectively. The measured MEm was 402 and 401 kJ $ME/kg^{0.75}/d$ and the calculated MEm from regression analysis was 398 and 400 kJ $ME/kg^{0.75}/d$ for S and A breeds, respectively. There were no significant differences between both measured and regression techniques. There was no significant difference between S and A breeds for DOMI, N retention, MEm, digestibility and metabolizability values. Digestibility values for OM, GE and CP and metabolizability were significantly (p<0.05) higher in restricted feeding sheep compared with that of sheep fed ad libitum.

• Journal of Photoscience
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• 제9권2호
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• pp.314-316
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• 2002

In halobacterial membrane, pharaonis phoborhodopsin (or pharaonis sensory rhdopsin II, psRII) forms a complex with its transducer pHtrII. Flash-photolyis of D75N mutant did not yield M-intermediate but an O-like intermediate is observed. We examined the interaction between D75N of ppR and t-Htr (truncated pHtrII). These formed a complex in the presence of n-dodecyl-$\beta$-D-maltoside, and the association accelerated the decay of the 0 of D75N from 15 to 56 s$\^$-1/. From the decay time constants under varying ratios of D75N and t-Htr, n, the molar ratio of D75N/t-Htr in the complex, and K$\_$D/, the dissociation constant, were estimated. The value of n was unity and K$\_$D/ was estimated to 146 nM. This K$\_$D/ value can be considered as the association between the photo-intermediate and t-Htr, which is deduced by the method of estimation. Previously we (Photochem. Photobiol. 74, 489-494 (2001)) reported K$\_$D/ of 15 $\mu$M for the interaction between the wild-type and t-Htr by means of the change of M-decay rates. Therefore, this value should be the K$\_$D/ value for the interaction between M of the wild-type and t-Htr.

• 한국항해항만학회지
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• 제27권4호
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• pp.359-366
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• 2003

이 논문은 대한민국 해양사고 예보 시스템 (K-MACFOS)을 개발하기 위한 해양사고 수량화 D/B (N-D/B) 구성과 분석에 관하여 기술하였다. K-MACFOS의 주목표는 일기예보와 같이 해양사고의 예측건수와 위험수준을 방송하기 위한 것이다. 해양사고 데이터는 1990년부터 2000년까지 1년간 위도 33oN∼35oN와 경도 124oE∼127oE의 대한민국 서남해안 일대에서 발생한 총 724건을 수집하였고, 14가지 수량화변환 척도를 이용하여 양적 데이터로 변환하였다. 컬러 콘도-맵 가시화를 이용한 통계분석을 통하여 N-D/B의 유효성과 연구대상 해역의 사고특징을 검토하였다. 또한, 올바른 N-D/B 분석과 정확한 해양사고 건수 예측을 위한 최적 적용기간 선정 방법을 제안하였다.

• 한국전자파학회논문지
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• 제13권1호
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• pp.94-100
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• 2002

본 논문에서는 N개의 평행 결합선로를 이용한 3 dB 결합기를 해석하였으며, 유도된 식에 의하여 최소의 결합선로 수와 크기로 설계하였다. 기존의 Spectral Domain상에서 N단 평행 결합선로의 4N-port의 산란행렬로부터 port reduction 방법을 사용하여 4-port 산란 행렬을 유도하였으며, 유도된 결과의 타당성을 검증하기 위하여 실제 제작하고 측정하였다. 제작된 결합기는 loose coupling의 평행 결합 선로를 이용하기 때문에 Lange Coupler 와 같은 높은 임피던스와 Tight coupling을 구현할 필요가 없으며, wire bonding포 용이하다. 최소의 단수로 구현하기 위해 높은 유전율과 두꺼운 기판을 사용하여 2단으로 3 dB 구현이 가능함을 제시하였다. 제작결과 3.6 GHz에서 5.5 GHz로 대략 42 %(0.5 dB unbalance) 정도의 광대역 특성을 가지고 위상차도 1$^{\circ}$내외의 결합기를 구현할 수 있었고 격리도 특성 또한 대역 내에서 15 dB 내외의 특성을 보였다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.173.2-173.2
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• 2003

Vanilloid receptor I (VR1) is a nonselective cation ion channel placed in the plasma membrane of peripheral sensory neurons that is potential target for analgesia A series of N-4-substituted-benzyl-N'-tert-butylbenzyl thioureas were prepared for the study of their agonistic/antagonistic activities to the vanilloid receptor in rat DRG. Their structure-activity relationship in reveals that not only the two oxygens and amide hydrogen of sulfonamido group but also the optimal size of methyl in methanesulfonamido group play an integral role for the antagonistic activity on vanilloid receptor.

• 대한수학회보
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• 제23권2호
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• pp.103-111
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• 1986

In [2] and [3], the Shinwon polynomial S$_{n}$(x) of order n is defined and studied in some details. In this paper we will define the general Shinwon polynomial S$_{n}$(a,x) and the Dickson polynomial D$_{n}$(a,x) of the second kind of order n which is a slightly changed form of the Dickson polynomial g$_{n}$(a,x), and show that D$_{n}$(a,x) is closely related to S$_{n}$(a,x).EX> n/(a,x).

• 대한수학회지
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• 제33권2호
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• pp.423-438
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• 1996

Let L be a linear functional on the vector space of polynomials in x. Let $\omega(x)$ be a polynomial in x of degree d, for some positive integer d. We consider two sets of polynomials, ${R_n (x)}_{n \geq 0}, {S_n(x)}_{n \geq 0}$, such that $R_n(x)$ is a polynomial in x of degree n and $S_n(x)$ is a polynomial in $\omega(x)$ of degree n. (So $S_n(x)$ is a polynomial in x of degree dn.)