• Title/Summary/Keyword: dCAPS

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A Gene-based dCAPS Marker for Selecting old-gold-crimson (ogc) Fruit Color Mutation in Tomato (토마토 과색 돌연변이 유전자(old-gold-crimson) 선발을 위한 dCAPS 분자표지 개발)

  • Park, Young-Hoon;Lee, Yong-Jae;Kang, Jum-Soon;Choi, Young-Whan;Son, Beung-Gu
    • Journal of Life Science
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    • v.19 no.1
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    • pp.152-155
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    • 2009
  • The old-gold-crimson ($og^c$) fruit color mutation produces deep red tomato fruit with high lycopene content. age is a null mutation allele of lycopene-${\beta}$-cyclase (Crt-b) gene (B locus) that converts lycopene to ${\beta}$-carotene in the cartenoid biosynthesis pathway in tomato. Breeding of high lycopene tomato cultivars can be accelerated by marker-assisted selection (MAS) for introgression of $og^c$ allele by using a gene-based DNA marker. In order to develop a marker, single nucleotide deletion of adenine(A) with. in a poly-A repeat that has been known to be responsible for frame-shift mutation of $og^c$ was confirmed by resequencing mutant allele and wild-type allele at B locus of several tomato lines. For allele discrimination and detection of $og^c$, derived CAPS (dCAPS) approach was used by designing a primer that artificially introduced restriction enzyme recognition site of Hin fI in PCR products from $og^c$ allele. This dCAPS marker is co-dominant gene-based PCR marker that can be efficiently used for MAS breeding program aiming the development of high lycopene tomato.

Strength Prediction of Concrete Pile Caps Using 3-D Strut-Tie Models (3차원 스트럿-타이 모델을 이용한 파일캡의 강도예측)

  • 박정웅;윤영묵
    • Proceedings of the Korea Concrete Institute Conference
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    • 2003.11a
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    • pp.380-383
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    • 2003
  • Deep pile caps usually contain no transverse shear reinforcement and only small percentages of longitudinal reinforcement. The current design procedures including ACI 318-02 for the pile caps do not provide engineers with a clear understanding of the physical behavior of deep pile caps. In this study, the failure strengths of nine pile cap specimens tested to failure were evaluated using 3-dimensional strut-tie models. The analysis results obtained from the present study were compared with those obtained from several design methods, and the validity of the present method implementing 3-dimensional strut-tie models was examined.

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Development of molecular marker for species authentication of Dendranthema indicum (L.) Des Moul. and D. boreale (Makino) Ling ex Kitam. (감국(Dendranthema indicum (L.) Des Moul.) 및 산국(D. boreale (Makino) Ling ex Kitam.)의 종판별 분자마커 개발)

  • Byeon, Jihui
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.66-66
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    • 2018
  • 국화과(Compositae) 다년생 초본인 산국속(Dendranthema)은 국내 약 13여종이 자생하는 것으로 알려져 있으며, 이 중 감국(D. indicum (L.) Des Moul.)과 산국(D. boreale (Makino) Ling ex Kitam.), 구절초(D. zawadskii var. latilobum (Maxim.) Kitam.)가 주로 차 또는 한약재 등의 원료로 이용되고 있다. 차로 이용되는 꽃은 산국이 감국에 비해 상대적으로 작아서 구분이 가능하지만 시중에는 건조된 형태로 가공 유통되므로 육안으로 구분이 쉽지 않고, 산국 유래 제품들은 국내에서 감국 또는 국화로 혼용해서 표기되어 유통되고 있어 그 기원을 명확히 정립할 필요가 있다. 이에 본 연구는 감국과 산국의 분자유전학적 판별을 위해 DNA 바코드 후보 유전자를 활용하여 염기서열분석으로 확보된 SNP 및 InDel 정보를 바탕으로 CAPS 마커를 개발하고자 수행되었다. 감국과 산국 모두 trnL-trnF intergenic spacer 구간에서 약 1kb의 PCR 산물이 확인되었고, 이들 염기서열에서 분석한 2 SNP 및 3 InDel을 대상으로 CAPS 마커 개발을 위한 제한효소 사이트를 탐색하였다. Gap을 포함한 774bp (감국/산국=A/G) 위치의 SNP에서 BstUI(GC^GC)처리로 CAPS 마커로 전환 가능함이 확인되었고, 이에 감국과 산국의 PCR 산물에 제한효소를 처리한 결과, 제한효소 인식 사이트가 존재하는 산국에서 두 개의 DNA 단편이 확인되었다. 위 결과는 다양한 형태로 가공 유통되는 감국과 산국의 판별을 위한 마커로 활용될 수 있으며, 본 연구에 활용된 기술은 추후 건강기능식품 개발을 위한 원료표준화 확립 연구에 유용할 것으로 판단된다.

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Validity Test for Molecular Markers Associated with Resistance to Phytophthora Root Rot in Chili Pepper (Capsicum annuum L.) (고추의 역병 저항성과 연관된 분자표지의 효용성 검정)

  • Lee, Won-Phil;Lee, Jun-Dae;Han, Jung-Heon;Kang, Byoung-Cheorl;Yoon, Jae-Bok
    • Horticultural Science & Technology
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    • v.30 no.1
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    • pp.64-72
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    • 2012
  • Phytophthora root rot has been causing a serious yield loss in pepper production. Since 2004, the year in which commercial cultivars resistant to the disease were firstly commercialized, it has been necessary to introduce the resistance into domestic pepper cultivars for dried red pepper. Therefore, developing molecular markers linked to the resistance is required for an accurate selection of resistant plants and increasing breeding efficiency. Until now, several markers associated with the major dominant gene resistant to Phytophthora root rot have been reported but they have some serious limitations for their usage. In this study, we aimed to develop molecular markers linked to the major dominant gene that can be used for almost of all genetic resources resistant to Phytophthora root rot. Two segregating $F_2$ populations derived from a 'Subicho' ${\times}$ 'CM334' combination and a commercial cultivar 'Dokyacheongcheong' were used to develop molecular markers associated with the resistance. After screening 1,024 AFLP primer combinations with bulked segregant analysis, three AFLP (AFLP1, AFLP2, and AFLP3) markers were identified and converted into three CAPS markers (M1-CAPS, M2-CAPS, and M3-CAPS), respectively. Among them, M3-CAPS marker was further studied in ten resistants, fourteen susceptibles, five hybrids and 53 commercial cultivars. As a result, M3-CAPS marker was more fitted to identify Phytophthora resistance than previously reported P5-SNAP and Phyto5.2-SCAR markers. The result indicated that the M3-CAPS marker will be useful for resistance breeding to Phytophthora root rot in chili pepper.

Refined 3-Dimensional Strut-Tie Models for Analysis and Design of Reinforced Concrete Pile Caps (철근콘크리트 파일캡의 해석 및 설계를 위한 개선 3차원 스트럿-타이 모델)

  • Kim, Byung Hun;Chae, Hyun Soo;Yun, Young Mook
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.1
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    • pp.115-130
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    • 2013
  • The sectional methods of current design codes have been broadly used for the design of various kinds of reinforced concrete pile caps. Lately, the strut-tie model approach of current design codes also became one of the attracting methods for pile caps. However, since the sectional methods and the strut-tie model approach of current design codes have been established by considering the behaviors of structural concrete without D-regions and two-dimensional concrete structures with D-regions, respectively, it is inappropriate to apply the methods to the pile caps dominated by 3-dimensional structural behavior with disturbed stress regions. In this study, the refined 3-dimensional strut-tie models, which consider the strength characteristics of 3-dimensional concrete struts and nodal zones and the load-carrying capacity of concrete ties in tension regions, are proposed for the rational analysis and design of pile caps. To examine the validity of the proposed models and to verify the necessity of appropriate constituent elements for describing 3-dimensional structural behavior and load-transfer mechanism of pile caps, the ultimate strength of 78 reinforced concrete pile caps tested to failure was examined by the proposed models along with the sectional and strut-tie model methods of current design codes.

A Study on the Pattern Making Theory for Steeve Cap Part of Sleeve Pattern Appropriate for the Characteristics of Arm Form (팔 형태특성에 적합한 소매패턴의 소매산부분 제도이론)

  • Cho, Kyeong-Hee;Machiko, Miyoshi
    • Journal of the Korean Society of Clothing and Textiles
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    • v.32 no.4
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    • pp.641-650
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    • 2008
  • In this study, seven models with distinctly different body types were researched and explanatory variables were reviewed. Review was done by using body measurements and the measurements of the appropriate bodice patterns in 1D, 2D, and 3D measurement methods in order to review the pattern making system and the equations for calculating the dimensions of the sleeve caps, which are readily available. Data on human bodies, which can be the criteria for each body part required for the new system for making sleeve cap part, were selected considering conditions such as items with a significantly high contribution rate from the results of regression analysis and the easiness of measurement. As a result of research, an explanatory variable required for the system for making sleeve caps with high general use was extracted. All items with the exception of the waist circumference and upper arm circumference were about the measurement of the form of human bodies, which were newly set in this study. As a result of this study, the equations for calculating the dimensions of each part of the sleeve caps showed distinct differences in comparison to the conventional system of construction.

Real-Time Automatic Target Detection in CCD image (CCD 영상에서의 실시간 자동 표적 탐지 알고리즘)

  • 유정재;선선구;박현욱
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.41 no.6
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    • pp.99-108
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    • 2004
  • In this paper, a new fast detection and clutter rejection method is proposed for CCD-image-based Automatic Target Detection System. For defence application, fast computation is a critical point, thus we concentrated on the ability to detect various targets with simple computation. In training stage, 1D template set is generated by regional vertical projection and K-means clustering, and binary tree structure is adopted to reduce the number of template matching in test stage. We also use adaptive skip-width by Correlation-based Adaptive Predictive Search(CAPS) to further improve the detecting speed. In clutter rejection stage, we obtain Fourier Descriptor coefficients from boundary information, which are useful to rejected clutters.

Development of Fiber-end-cap Fabrication Equipment (대구경 광섬유 엔드캡 제작장비 개발)

  • Lee, Sung Hun;Hwang, Soon Hwi;Kim, Tae Kyun;Yang, Whan Seok;Yoon, Yeong Gap;Kim, Seon Ju
    • Korean Journal of Optics and Photonics
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    • v.32 no.2
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    • pp.49-54
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    • 2021
  • In this paper, we design and construct the equipment to manufacture large-diameter optical fiber end caps, which are the core parts of high-power fiber lasers, and we fabricate large-diameter optical fiber end caps using the home-made equipment. This equipment consists of a CO2 laser as a fusion-splice heat source, a precision stage assembly for transferring the position of a large-diameter optical fiber and an end cap, and a vision system used for alignment when the fusion splice is interlocked with the stage assembly. The output of the laser source is interlocked with the stage assembly to control the output, and the equipment is manufactured to align the polarization axis of the large-diameter polarization-maintaining optical fiber with the vision system. Optical fiber end caps were manufactured by laser fusion splicing of a large-diameter polarization-maintaining optical fiber with a clad diameter of 400 ㎛ and an end cap of 10×5×2 ㎣ (W×D×H) using home-made equipment. Signal-light insertion loss, polarization extinction ratio, and beam quality M2 of the fabricated large-diameter optical fiber end caps were measured to be 0.6%, 16.7 dB, and 1.21, respectively.

The Domestic Trend of Studies on 3D Printing Technology Applied to the Fashion Industry

  • Choi, Jeongwook;Park, Sanghee
    • Journal of Fashion Business
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    • v.24 no.6
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    • pp.80-88
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    • 2020
  • This study, by analyzing previous studies, aimed to understand how 3D printing technology is applied and utilized in the fashion industry and to contribute to encouraging further studies on 3D printing technology in the fashion sector and suggesting proper ways for designing such studies. Firstly, 47 papers were selected from all literature concerning 3D printing technology published in 15 journals of fashion and design since 2013. Afterwards, these papers were analyzed with regard to the frequency, topics or sectors, and purposes or types of studies shown by outcomes. Results were as follows: First, the number of papers on 3D printing technology published in the journals was counted according to the year, which showed that this number increased rapidly after 2015 for about 3 years. Especially in the year 2016, this increase was quite notable. Although a little decrease in this number was found afterwards, a steady increase was highly expected. Out of the 8 journals, Journal of The Korean Society of Fashion Design had the maximum papers. Regarding areas of studies, works on designing and development of products were most common. Finally, regarding the purpose of studies, those suggesting or presenting apparel were predominant. Product items included clothes, shoes, and caps. Studies on caps mostly covered designing the products. Studies on clothes aimed at designing, characteristics of construction, and case study. However, there were very few works on 3D printing technology as an alternative material or composition of clothing.

The change in C8 and C9 volatile compounds according to the drying conditions of Pleurotus citrinopileauts and P. djamor (노랑느타리와 분홍느타리의 건조조건에 따른 C8과 C9 향기성분의 변화)

  • Minji Oh;Minseek Kim;Ji-Hoon Im;Youn-Lee Oh
    • Journal of Mushroom
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    • v.21 no.4
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    • pp.222-227
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    • 2023
  • Mushrooms have a unique taste and aroma, so in the processing of mushroom products with other ingredients, a separate pre-processing step is often taken to eliminate the mushroom aroma. In this study, we analyzed the changes in the concentration of volatile compounds according to drying conditions to promote the activation of processing using the fruiting bodies of yellow oyster mushrooms(Pleurotus citrinopileatus) and pink oyster mushrooms(P. djamor). The caps and stipes of yellow oyster and pink oyster mushrooms were separated and freeze-dried at -70℃ for 120 hours. Subsequently, they were hot air-dried at temperatures of 40, 50, 60, and 70℃ for 24, 24, 16, and 12 hours, respectively. The dried samples were pulverized and quantitatively analyzed by SPME-GC-MS. In the case of yellow oyster mushrooms, the concentration of t-2-nonenal in caps and stipes during freeze-drying was 164.43 ㎍/g d.w. and 174.80 ㎍/g d.w., respectively, whereas during hot air-drying, it significantly decreased to 0.35~3.41 ㎍/g d.w. and 0.98~59.88 ㎍/g d.w. In a similar manner, for pink oyster mushrooms, the concentration of 1-octen-3-ol during freeze-drying in caps and stipes was 31.05 ㎍/g d.w. and 176.17 ㎍/g d.w., respectively, whereas during hot air-drying, it significantly decreased to 1.59~9.66 ㎍/g d.w. and 1.96~15.77 ㎍/g d.w. Furthermore, most volatile compounds showed a tendency to decrease in concentration as the temperature during hot air-drying increased.