• Biomolecules & Therapeutics
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• v.11 no.2
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• pp.112-118
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• 2003

1-Methylated $\beta$-carbolines (harmaline and harmalol) and antioxidants (N-acetylcysteine and ascorbate) reduced the loss of cell viability in differentiated PC 12 cells treated with 5 mM glutamate. $\beta$-Carbolines prevented the glutamate-induced decrease in mitochondrial membrane potential, cytochrome c release and caspase-3 activation in PC 12 cells. $\beta$-Carbolines reduced the formation of reactive oxygen species and depletion of glutathione due to glutamate in PC12 cells. $\beta$-Carbolines revealed a scavenging action on hydrogen peroxide and reduced the iron and EDTA-mediated degradation of 2-deoxy-D-ribose. The results suggest that I-methylated $\beta$-carbolines attenuate the cytotoxic effect of glutamate on PC12 cells by reducing the alteration of mitochondrial membrane permeability that seems to be mediated by oxidative stress.

• Animal Systematics, Evolution and Diversity
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• v.36 no.1
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• pp.7-9
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• 2020

In the present study, the first mitochondrial cytochrome c oxidase subunit I gene (COI) sequence of Sterkiella multicirrata Li et al., 2018 is presented. To begin with, this species has been also morphologically recorded from South Korea, and this study was performed using genomic DNA of the Korean population. The newly obtained COI sequences of S. multicirrata were identical. And the inter-specific variation between S. multicirrata and S. histriomuscorum was noted at 14.3%. These values correspond well with the results of previous studies. However, because there are very few available COI sequences of stichotrichian in GenBank, it is concluded that continuous accumulation of data is needed for further study.

• The Korean Journal of Physiology and Pharmacology
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• v.10 no.4
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• pp.207-212
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• 2006

Mitochondrial permeability transition has been shown to be involved in neuronal cell death. Mitochondrial monoamine oxidase (MAO)-B is considered to play a part in the progress of nigrostriatal cell death. The present study examined the effect of MAO inhibitors against the toxicity of 1-methyl-4-phenylpyridinium $(MPP^+)$ in relation to the mitochondrial permeability transition. Chlorgyline (a selective inhibitor of MAO-A), deprenyl (a selective inhibitor of MAO-B) and tranylcypromine (nonselective inhibitor of MAO) all prevented cell viability loss, cytochrome c release, caspase-3 activation, formation of reactive oxygen species and depletion of GSH in differentiated PC12 cells treated with $500\;{\mu}M$$MPP^+$. The MAO inhibitors at $10\;{\mu}M$ revealed a maximal inhibitory effect and beyond this concentration the inhibitory effect declined. On the basis of concentration, the inhibitory potency was tranylcypromine, deprenyl and chlorgyline order. The results suggest that chlorgyline, deprenyl and tranylcypromine attenuate the toxicity of $MPP^+$ against PC12 cells by suppressing the mitochondrial permeability transition that seems to be mediated by oxidative stress.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.1
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• pp.79-83
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• 2014

Thirty five juveniles belonging to the genus Collichthys were collected using a bag net at Gang-wha-do, in the eastern Yellow Sea, between July and September, 2012, and identified using combined genetic and morphological methods. We sequenced 316 base pairs of mitochondrial DNA cytochrome c oxidase subunit I of 35 individuals, of which 22 individuals were identified as Collichthys niveatus (12.9-47.6 mm in SL) and 13 as Collichthys lucidus (13.4-40.3 mm SL). Morphologically, the number of occipital crests, an important taxonomic character during the adult stage, could not distinguish the two species during the juvenile stage, but the shape of the first anal fin spine clearly distinguished the two species even among juveniles.

• Archives of Pharmacal Research
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• v.12 no.3
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• pp.196-200
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• 1989

The methanolic extract of the Rhizome of Curcuma zedoaria exhibited a significant prolongation of hexobarbital (HB)-induced hypnosis. Through liquid chromatography of an ether soluble fraction. monitoring by bioassay, three sequiterpenes, germacrone (A), curzerenone (B) and germacrone epoxide (C) were isolated as active consituents. A single treatment (100-200 mg/kg, i.p.) of each compound showed not only a significant prolongation of HB-induced sleeping time but also a significant inhibition of aminopyrine N-demethylase activity in mice, and further exhibited a typical type I binding spectra with oxidized rat hepatic cytochrome P-450 induced by phenobarbital. All of the compounds provoked a sleep episode at a subhypnotic dose of HB, implying that they possess CNS depressant properties.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.3
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• pp.411-416
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• 1997

To examine the free radical scavenging activities from buckwheat, Suwon No. 5 was extracted with various solvents. The seed of Suwon No. 5 had a high contents of carbohydrate, but the vegetable of that had a 31.4% of protein, 28.6% of ash and 25.7% carbohydrate as moisture free basis. Phenols contents of vegetable extracts had higher than those of seed extracts. Acetone extract of vegetable showed the greatest electron donating ability. Butanol and acetone extracts of vegetable showed high inhibitory effect on lipid peroxidation. Acetone extract of vegetable has also excellent activity in the superoxide radical scavenging activity by xanthine/xanthine oxidase-cytochrome c reduction system. The inhibitory effects of extracts on xanthine oxidase were measured. Acetone extract had the strongest inhibitory effect on xanthine oxidase and $IC_{50}$ was 2.2$\mu\textrm{g}$.

• Fisheries and Aquatic Sciences
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• v.19 no.2
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• pp.10.1-10.5
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• 2016

Setarches longimanus (Alcock 1894), in the family Scorpaenidae, was collected from Busan and the coastal waters of Jeju Island, Korea, in January and November 2014, respectively. Two specimens are characterized by XI, 10 dorsalfin rays; 19-21 pectoralfin rays; III, 5 anal fin rays; a second preopercular spine much shorter than the first and third; and an interorbital width 11.3 %-11.6 % of the standard length. We also analyzed 587 base pairs of the mitochondrial DNA cytochrome c oxidase subunit I sequences in order to confirm the taxonomic status of the specimen. As a result, the sequences of our specimen almost corresponded to those of Chinese S. longimanus (genetic distance, d = 0.005), but considerably differed from those of S. guentheri (d = 0.120-0.124). We propose the new Korean names "Ma-su-gam-peng-sok" for the genus Setarches, and "Bul-geun-ma-su-gam-peng" for the species S. longimanus.

• YAKHAK HOEJI
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• v.27 no.4
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• pp.295-301
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• 1983

Among the many protein modifications methylation is being investigated actively with regard to bacterial chemotaxis, gene regulation, muscle contraction, cytochrome c methylation, and the synthesis of the acyl transporter, carnitine. In this study the activities of protein methylase I, II, and III in pancreatic tissues of rat, mouse, and guinea pig were examined. Furthermore, the effect of cholinergic agents on the activity of protein methylases in pancreatic fragment of guinea pig was also examined in order to test the relationship between protein methylation and pancreatic secretion. The results are as follows. 1) The activities of protein methylases were generally high in pancreatic tissues of guinea pig and mouse but low in the tissue of rat. 2) The cholinergic stimulants, acetylcholine and carbachol at a concentration of $10^{-5}M$ decreased the activities of protein methylase I, II, and III compared with unstimulated control. 3) The inhibitory effect of the cholinergic stimulant on the activities of protein methylases was not blocked by atropine at a concentration of $10^{-5}M$.

• Archives of Pharmacal Research
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• v.29 no.5
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• pp.343-347
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• 2006

A new flavanone Persinol (1) and the new flavanone glucosides persinosides A (2) and B (3), along with known flavonoids (4 and 5) have been isolated from the ethyl acetate soluble fraction of the whole plants of Aerva persica. Their structures were elucidated on the basis of extensive analysis of nuclear magnetic resonance (1D & 2D-NMR) spectral data. All of them showed profound antioxidative activities by DPPH and cytochrome-c-reduction assays using the HL-60 cell culture system.

• Korean Journal of Clinical Laboratory Science
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• v.42 no.1
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• pp.46-54
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• 2010

Ceramide induces cell death in a variety of cell types however, the underlying molecular mechanisms related to renal epithelial cells remain unclear. The present study was undertaken to determine the role of extracellular signal-regulated protein kinase (ERK) in ceramide-induced cell death in renal epithelial cells. An established renal proximal tubular cell line of opossum kidney (OK) cells was used for this research. Ceramide induced apoptotic cell death in these cells. Western blot analysis showed that ceramide induced activation of ERK. The ERK activation and cell death induced by ceramide were prevented by the ERK inhibitor PD98059. Ceramide caused cytochrome C release from mitochondria into the cytosol as well as activation of caspase-3. Both effects were prevented by PD98059. The ceramide-induced cell death was also prevented by a caspase inhibitor. These results suggest that ceramide induces cell death through an ERK-dependent mitochondrial apoptotic pathway in OK cells.