• 한국동물학회지
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• 제29권3호
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• pp.171-180
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• 1986

단백질 분해효소, neuraminidase 및 EDTA가 아메바의 배양기표면 흡착, 세포표면의 미세구조 및 생화학적 조성에 미치는 영향을 concanavalin A(con A) cytochemistry 및 SDS PAGE에 의해 조사하였다. Con A cytochemistry에 의해 세포표면 바깥쪽의 filamentous(F)층과 안쪽의 amorphous(A)층이 쉽게 구분되었다. Neuraminidase로 처리한 아메바는 대조군에 비해 용기표면 흡착성과 펴짐이 증가하였으며 A층과 F층에 더 많은 con A결합부위가 노출되었다. Trypsin 및 proteinase K로 처리한 아메바는 각각 12시간, 48시간동안 용기표면에 부착하지 못하였으며, proteinase K의 처리는 A층의 con A결합부위 및 모든 glycoprotein을 제거시키는 효과를 낳았으며, trypsin은 세포막의 PAS염색물질에는 아무런 변화를 초래하지 않았으나 A층과 F층의 con A결합부위를 제거하였다. 이들 효소 및 EDTA처리에 의해 세포 표면의 mucopolysaccharide 일부가 분리되었다. 아메바를 monovalent con A로 처리하였을 때도 아메바는 용기표면에 부착하지 못하고 cytolysis되었다. 이상의 결과로 아메바의 용기표면 흡착에는 세포막의 glycoprotein과 A층의 mucopolysaccharide간의 상호작용에 의해서 이루어지는 것으로 보인다.

• Applied Microscopy
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• 제9권1호
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• pp.79-86
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• 1979

• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
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• pp.183.2-183
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• 2000

No Abstract, See Full Text

• 대한세포병리학회지
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• 제9권1호
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• pp.1-14
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• 1998

The cytologic distinction of carcinoma cells from reactive mesothelial cells can be difficult, especially in specimens containing abundant reactive mesotheilal cells and inflammatory cells with scant carcinoma cells. This study evaluates the usefulness of mucin and immunocytochemistry for discrimination between reactive mesotheilal cells and carcinoma cells, and sensitivity and specificity of these stains for the detection of metastatic carcinoma in serous effusions. Immunocytochemical panel including mucin cytochemistry with the periodic acid-Schiff(PAS) reaction after or without diastase digestion was undertaken on 127 serous effusion specimens with histologically confirmed diagnoses. The specimens including cell smears and cell blocks were stained with PAS and antibodies to carcinoembryonic antigen(CEA), epithelial membrane antigen(EMA), cytokeratln(CK), and vimentin. The sensitivities of these stains for metastatic carcinoma(127 cases) were 49%(46/94) in PAS, 48%(60/124) in CEA, 89%(97/109) in EMA, 88%(93/106) in CK, and 25%(20/81) in vimentin. The sensitivities of stains for reactive mesothelial cells(36 cases) were 19%(7/36) in EMA, 78%(28/36) in CK, and 75%(27/36) in vimentin. The PAS and CEA stains were not reacted with all cases of benign reactive serous effusions containing abundant reactive mesothelial cells. The specificities of stains for metastatic carcinoma(127 cases) were 100% in PAS, 100% in CEA, 81% in EMA, 22% in CK, and 25% in vimentin. The optimal combination of stains for use in a panel was PAS and CEA. Combined results from these two stains yielded an advanced sensitivity of 8% in PAS and 4% in CEA for metastatic carcinoma. EMA was also cosiderably useful for identification of carcinoma cells. CK and vimentin were not suitable for distinguishing between reactive mesothelial cells and carcinoma cells.

• Journal of Microbiology and Biotechnology
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• 제9권6호
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• pp.832-838
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• 1999

Two different types of lipases (lipase I and lipase II) secreted into culture medium by Rhizopus sp. L-I were purified using a hydrophobic chromatography and were partially characterized. Both enzymes were monomeric as revealed by SDS-PAGE and gel filtration. The molecular masses of the enzymes were identified as 45 kDa (lipase I) and 69 kDa (lipase II). The isoelectric points were estimated to be 3.6 and 5.2 for lipase I and lipase II, respectively. pH and temperature activity optima for lipase I were as 7.5 and $50^{\circ}C$, respectively, whereas the corresponding parameters for lipase II were 6.0 and $45^{\circ}C$. The amino terminal sequences of lipase I and lipase II, determined by Edman degradation, were found to be Leu-Val-Met-Ile-Gln-Arg and Leu-Val-Met-Lys-Gln-Arg, respectively. By western blotting analysis, the two lipases were found to have a common antigenic determinant. Immuno-electron cytochemistry conducted with polyclonal anti-lipase I antibody indicated the enzyme located in both the periplasm and the adjacent vesicles of fungal hyphae. Fortunately, the sites on the cell envelope where lipase was exported into the culture medium was also identified.

• 한국전자현미경학회:학술대회논문집
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• 한국현미경학회 2007년도 제38차 춘계학술대회 초록집
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• pp.75-78
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• 2007

The localization of cellulase were investigated in the capitate glandular trichomes of Pelargonium ${\times}$ fragransby a transmission electron microscopy. The secretory cells of capitate trichomes involved in biosynthesis and its secretion. Secretory material is transported to the space between the plasma membrane and cell wall, and subsequently accumulated in the secretory cavity. The splitting of secretory cell wall during the formation of secretory cavity is suggested that wall-forming enzymes, such as cellulase, may contribute to the wall separation process. Cellulase reaction product was localized in the secretory cell, the secretory cavity and in the subcuticular wall of glandular trichomes. Reaction products were present over fibrillar matrix in the secretory cavity associated with both the inner wall and the subcuticular wall.

• Applied Microscopy
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• 제12권1호
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• pp.11-21
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• 1982

A number of recent ultrastructural studies have shown marked differences between the two lining cell types in adult liver sinusoids, endothelial cells and Kupffer cells. In the present study, the ultrastructural features and electron microscopic cytochemistry of sinusoidal lining cells in the fetal liver were studied through fetal period to neonate in the rat. At fetal period, the sinusoid, which contains various blood component, in lined by the endothelial cells, the Kupffer cells and the fat storing cells that located in the space of Disse. As gestation proceeded, these eel's are arranged as adult liver sinusoids. The sinusoidal wall appears to be discontinuous with open fenestration between endothelial cells, but no basal lamina can observed. It seems to be morphologically and functionally distinct at the early gestation between the endothelial cells and the Kupffer cells, the latter showing marked phagocytized activity. The fat storing cells, which contain several fat droplets, are located in the space of Disse. Ultrastructural localization of the acid and alkaline phosphatase activity were noted on the sinusoidal lining cells.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.119.1-119
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• 2003

The glyoxysomal nature of microbodies was determined in Botryosphaeria dothidea hyphae based on morphology and in situ enzyme characteristics by transmission electron microscopy and cytochemistry. Bound by a single membrane, microbodies had a homogeneous matrix and varied in size ranging from 200 to 400 m in diameter. Microbodies had crystalline inclusion(s) which consisted of parallel arrays of fine tubules in their matrices. Microbodies and lipid globules were frequently placed in close association with each other, forming microbody-lipid globule complexes in hyphae. The cytochemical activities of catalase and malate synthase were localized in matrices of microbodies, showing intense electron-density of the organelle. In addition, the immunogold labeling detected the presence of catalase in multivesicular bodies and hyphal cell walls as well as in matrices and crystalline inclusions of microbodies, supporting the enzyme secretion through cell walls. Meanwhile, isocitrate Iyase was localized only in matrices of microbodies. These results suggest that microbodies, particularly complexed with lipid globules, in the fungal hyphae are functionally defined as glyoxysomes, where glyoxysomal enzymes are biochemically active for the glyoxylate cycle to be a metabolic pathway in gluconeogenesis. (Mycology and Fugus Diseases)

• Applied Microscopy
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• 제28권3호
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• pp.399-414
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• 1998

Glandular trichomes present on the leaf surface of Drosera capensis were examined using transmission electron microscopy. A large number of stalked glands exist on the adaxial surfaces of the leaf blade. The secretory head is composed of two layers of secretory cells, one layer of middle cells, and the inner tracheids. The secretory cells contain rough endoplasmic reticulum, mitochondria, plastids, Golgi apparatus, and vacuoles. The secretory cells show prominent cell wall ingrowth, and thick cuticle restricted on the subcuticular wall. Frequently, the cuticle has some pores, canal-like structures, showing electron -dense granules being penetrated through them. Ultrastructural localization using diaminobenzidine showed the electron-dense deposits in the vacuole. No peroxidase activity was seen in the cell wall and cytolasm. The activity of peroxidase (POX) isozymes in Drosera which isoelectric point (pI) is 3.6 and some anionic POX isozymes which pIs are laid between 3.6 and 4.6 were especially increased according to the development and the formation of glandular trichomes. Also, the activity of some POX isozymes which isoelectric points are laid between 4.6 and 5.1 were increased in the regions of leaves which has trichomes.

• 대한의생명과학회지
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• 제7권2호
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• pp.71-77
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• 2001

To evaluate the effect of occlusive skin on the activity of cutaneous oxygen free radical metabolizing enzymes in rats, the dorsal skin was covered with closed glass chamber shaped petri dish, 46 mm in diameter and 10 mm in height and sealed by an adhesive. Five day-occluded group showed more increased activity of xanthine oxidase (XO) than that of control, and the activity of five day-occluded group was higher than that of ten day-occluded group. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were significantly higher in ten day-occluded group than in control or five day-occluded group. All the more, five day-occluded group showed the decreasing tendency of SOD and GPx activities compared to those of control. On the other hand, the cerrous perhydroxide deposits were observed in the intercellular space of the stratum basale in five day-occluded group under the electronic microscope using a cytochemistry method. Futhermore, the degree of cerrous perhydroxide reaction was lower in ten day-occluded group than in five day-occluded group. In conclusion, the increased XO activity and the decreased SOD and GPx activities are likely to responsible far the accumulation of $H_2O_2$ in five day-occluded group.