Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Extracellular Triacylglycerol Lipases Secreted by New Isolate of Filamentous Fungus

  • Lusta, Konstantin A. (Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Faculty of Life Resources, Catholic University of Taegu-Hyosung) ;
  • Woo, Sahng-Young (Faculty of Life Resources, Catholic University of Taegu-Hyosung) ;
  • Chung, Il-Kyung (Faculty of Life Resources, Catholic University of Taegu-Hyosung) ;
  • Sul, Ill-Whan (Institute of Agricultural Technology and Science, Kyungpook National University) ;
  • Park, Hee-Sung (Faculty of Life Resources, Catholic University of Taegu-Hyosung) ;
  • Shin, Dong-Ill (Faculty of Life Resources, Catholic University of Taegu-Hyosung)
  • Published : 1999.12.01
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Abstract

Two different types of lipases (lipase I and lipase II) secreted into culture medium by Rhizopus sp. L-I were purified using a hydrophobic chromatography and were partially characterized. Both enzymes were monomeric as revealed by SDS-PAGE and gel filtration. The molecular masses of the enzymes were identified as 45 kDa (lipase I) and 69 kDa (lipase II). The isoelectric points were estimated to be 3.6 and 5.2 for lipase I and lipase II, respectively. pH and temperature activity optima for lipase I were as 7.5 and $50^{\circ}C$, respectively, whereas the corresponding parameters for lipase II were 6.0 and $45^{\circ}C$. The amino terminal sequences of lipase I and lipase II, determined by Edman degradation, were found to be Leu-Val-Met-Ile-Gln-Arg and Leu-Val-Met-Lys-Gln-Arg, respectively. By western blotting analysis, the two lipases were found to have a common antigenic determinant. Immuno-electron cytochemistry conducted with polyclonal anti-lipase I antibody indicated the enzyme located in both the periplasm and the adjacent vesicles of fungal hyphae. Fortunately, the sites on the cell envelope where lipase was exported into the culture medium was also identified.

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