• Title/Summary/Keyword: cystine

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Affinity of Mercury to SH Compounds Compared by Using the Paper Chromatography (Paper Chromatogaphy 법(法)에 의한 SH화합물간(化合物間)의 수은(水銀)에 대(對)한 친화력(親和力) 비교(比較))

  • Kim, Young-Hee;Lee, Man-Jeang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.10 no.1
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    • pp.67-76
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    • 1981
  • By using the paper chromatograph technique affinity of amino acids to the mercury was compared with that of sodium thiosulfate, 2,3-dimercaptopropanol(BAL) and inosinic acid. Among the amino acids of testment three acids (Cysteine, Cystine and Methionine) which posses sulfide or disulfide radical exhibited a spot which combined more apparently with the mercury than other amino acids. However, in the mixed solutions of thiosulfate, BAL or inosinic acid with those amino acid and mercury, the mercury was moved into thiosulfate, BAL or inosinic acid spot. It was clear from the results that affinity of sodiume thiosulfate, BAL and inosinic acid to the mercury is higher than that of amino acids.

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The Taste Compounds of Fermented Oyster, Crassostrea gigas ( I ) -Changes of Free Amino Acids during the Fermentation of Oyster- (굴젓의 정미성분(呈味成分) ( I ) -굴젓숙성중(熟成中)의 유리(遊離)아미노산(酸)의 변화(變化)-)

  • Chung, Seung-Yong;Lee, Jong-Mee;Lee, Jong-Ho;Sung, Nak-Ju
    • Journal of Nutrition and Health
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    • v.10 no.4
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    • pp.97-103
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    • 1977
  • Changes of free amino acids as taste compounds during the fermentation of oyster were analyzed by amino acid autoanalyzer. In fresh oyster, taurine, glutamic acid and alanine were abundant amino acids and the amounts of taurine (731mg%, on moisture and salt free base), glutamic acid (365mg%) and alanine (345.4mg% ) were 63.8% of the total free amino acids. Cystine, isoleucine, phenylalanine, leucine and histidine were detected as less abundant free amino acids and the amount of those amino. acids ranged from 5. 5mg% (cystine) to 32.9mg% (histidine). The free amino acids analyzed in this experiment were not changed in composition hut changed in amounts during 124 days of fermentation. Aspartic acid and leucine were continually increased during 124 days of fermentation. Lysine, histidine, threonine, serine, glutamic acid, tyrosine and phenyalanine were increased unlit 68 days of fermentation and than decreased gradually. The increase of arginine, glycine, valine and isoleucine were fluctuated. Taurine were dramatically decreased during the 124 days of fermentation. It is believed that glutamic acid, alanine, lecuine, serine, Iysine and threonine play an important role as taste compounds in fermented oyster because those amino acids were most abundant in fermented oyster.

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Effects of Sulfur Nutritional Forms on Accumulation of Seed Storage Proteins in Soybean (Glycine max)

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    • Korean Journal of Plant Resources
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    • v.10 no.3
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    • pp.221-226
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    • 1997
  • Improvement of seed protein quality might be an essential issus in soybean and would give more profit directly to both farmers and users. This study was carried out to investigate the effects of reduced-S form(s) on seed storage protein components in soybean during seed filling stages. The reduced-S forms during seed fill were sodium thiosulfate, sodium sulfite, sodium sulfide, thioaceteat, $\beta$-mercaptoethanol, thiourea, thiamine-HCI, L-cysteine, L-cystine, and L-methionine. Seed storage protein concentration did not appear to be affected by any reduced-S forms. However, glycinin and $\beta$-conglycinin concentration seemed to be changed greatly by L-methionine. This resulted in the increase in the 11S/7S ratio(3.58). Among the $\beta$-conglycinin, $\beta$-subunit was not accumulated at all. $\alpha$-subunit concentration appeared to be decreased and $\alpha'$-subunit concentration was not altered in comparison with sulfate control. Also, $\beta$-conglycine concentration, especially $\beta$-subunit concentration, tended to be decreased with L-cystine treatment, resulting in an increase in the 11S/7S ratio(1.83). The glycinin concentration tended to be increased at the expense of the decrease in the $\beta$-conglycinin concentration. Therefore, it is suggested that enhancing soybean protein quality would be achieved by improving metabolic pathways of S assimilation in soybean plants during seed filling period under sulfate-sufficient condition.

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The Investigation on Color Change of Dis-azo Acid Dye in Wool Dyeing (양모섬유의 염색시 디스아조계 산성염료의 변색현상 규명)

  • 김미경;김태경;윤석한;임용진
    • Textile Coloration and Finishing
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    • v.15 no.2
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    • pp.86-92
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    • 2003
  • It is already known that the color of wool fabric dyed with disazo acid dyes could be changed in dyeing process and this is accelerated under alkaline condition. Focus was given to figuring out the mechanism of this color change, through the LC-MS analysis. In this study, no color change was seen in wool fabrics dyed with C. I. Acid Blue 113 under weak acidic, neutral and weak alkaline conditions for 1hour. However, the wool fabrics dyed under weak alkaline condition for a long time over 3 hours fumed reddish orange. When the wool fabrics dyed under weak acidic, neutral and weak alkaline conditions were treated with $0.5g/L\;Na_2C0_3$ solution, all of them turned reddish orange. On the other hand, the color of silk fabrics dyed with C. I. Acid Blue 113 were not changed after the same alkaline treatment. Wool contains cystine and cysteine, whereas silk does not. Due to the reversible reduction/oxidation process of cystine and cysteine in wool dyeing, the C. I. Acid Blue 113 of the dis-azo type is decomposed by reduction and consequently turned them into the reddish orange mono-azo types dye.

Studies on the Amino Acid Constituents of Gynostemma pentaphyllum Makino (돌외 (Gynostemma pentaphyllum Makino)의 아미노산(酸) 조성(組成)에 관(關)한 연구(硏究))

  • Kim, Sun-Hee;Park, Won-Ki
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.17 no.1
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    • pp.7-11
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    • 1988
  • This study was carried out in order to analyse the amino acid constituents of Gynostemma pentaphyllum Makino (G. P. M. ) produced in Korea and Japan. Total and free amino acids of those were analysed by HPLC. Total amino acid contents of G. P. M. produced in Korea were similar to that of produced in Japan. The Histidine content of G. P. M. produced in Korea was more rich than that of produced in Japan. The content of glutamic acid was the most rich among in kinds of amino acids in both hind of G. P. M. G. P. M. in Korea contained the least methionine and G. P. M. from Japan contained the least cystine.

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Food Components and Volatile Flavors in Rhodiola sachalinensis Roots (홍경천(Rhodiola sachazinensis) 뿌리의 식품학적 성분 및 휘발성 향기성분)

  • 이은정;임지순;박채규;전병선;김석창
    • Food Industry And Nutrition
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    • v.9 no.1
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    • pp.53-57
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    • 2004
  • 홍경천(Rhodiola sachalinensis)의 성분을 분석하여 식품학적 특성을 검토하고 식품소재로서의 기초자료로 활용할수 있도록 건조된 홍경천을 분쇄, 분말화한 후 일반성분 및 무기성분, 유리당, 구성아미노산, 유리지방산, 그리고 향기성분을 분석하였다. 홍경천 건조분말의 수분은 10.14%, 조단백 3.90%, 조지방 1.33%, 총당 41.17%, 환원당 11.40% 회분은 3.05%로 분석되었고, 무기성분은 Ca, K, Kg이 높은 함량으로 나타났다. 유리당으로는 glucose, fructose, sucrose 등으로 특히 glucose와 fructose가 높게 함유되어있었다. 주된 구성아미노산은 glutmic acid, cystine arginine, proline, aspartic acid, isoleucine, histidine 등의 함량 순으로 나타났으며, 특히 glutamic acid, cystine, arginine 함량이 각각 176.24, 168.60, 159.90 mg%로 높은 함량 수준을 보였다. 유리지방산은 linoleic acid가 32.46%로 함량이 가장 높았으며 그 다음으로 palmitic acid가 21.42%, lignoceric acid 14.83%, oleic acid 13.09% behenic acid가 5.80% 순으로 나타났다. 향기성분은 GC-MS로 분석한 결과 2-cinnamic aldehyde 함량이 가장 높았으며 geraniol, myrtenol, octanol 등의 함량이 높게 나타났다. 이러한 결과로 볼 때 홍경천 뿌리는 다양한 일반성분 조성과, 필수아미노산, 불포화지방산, 필수 무기질 등이 균형있게 함유된 식품학적 성분 가치가 충분한 소재임이 확인되었다. 따라서 향후 홍경천 뿌리에서 유효성분을 추출하여 생리활성 및 동물실험을 통해 이들 식품학적 성분들의 유용성을 탐색하는 작업이 필요하리라 사료된다.

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Studies on the Changes of Amino acid Contents on Pleurotus ostreatus (느타리버섯의 배지별 Amino acid 함량변화에 관한 연구)

  • Ryu, Jeong;Lee, Gong-Joon;Jung, Gi-Tai;Na, Jong-Seong
    • The Korean Journal of Mycology
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    • v.22 no.4
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    • pp.338-342
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    • 1994
  • Amino acids of Pleurotus ostreatus in various cultural media(rice straw, sawdust and cotton waste) were analyzed to recognize the compositional differences depending on pileus size and portions(pileus and stipe) Total amino acids of rice straw were a little less than that of sawdust and cotton waste. Seventeen amino acids were identified and quantified. Among the amino acids glutamic acid was higest and cystine was lowest. The amino acid contents depending on the pileus size was higest in $3{\sim}7\;cm$ of rice straw, under 3 cm of sawdust, over 7 cm of cotton waste, respectively. The content of them was found to be higher in pileus than stipe.

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동물(動物) 부위(部位)에 따르는 수용성유리(水溶性遊離) Amino산(酸)의 분포(分布)에 대(對)한 연구(硏究) 제1보(第1報) (면양(緬羊)의 근육(筋肉)에 대(對)해서)

  • Yun, Hyeong-Sik;Song, Tae-Hwa;Park, Wan-Gil
    • Applied Biological Chemistry
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    • v.6
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    • pp.29-31
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    • 1965
  • The distribution of water soluble free amino acids in various parts of sheep muscles were observed. amino acids of cystine, histidine, aspartic acid, arginine, hydroxy proline, threonine, glutamic acid alanine, proline, methionine, tryptophan, leucine, iso-leucine, glycine, creatine, and two or four different kinds of amino acid content in each tissues, M. longissimus. Overall, the distribution of content of amino acids are seemed to be different for each tissues, while for hydroxyproline the result was negative.

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Studies on the Media Development of Pleurotus ostreatus by Waste Cotton Stuff (느타리버섯 배지개발을 위한 폐면포 이용에 관한 연구)

  • Ryu, Jeong;Lee, Gong-Joon;Jung, Gi-Tai;Na, Jong-Seong;Hwang, Chang-Ju
    • The Korean Journal of Mycology
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    • v.24 no.3 s.78
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    • pp.176-179
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    • 1996
  • Media development of Pleurotus ostreatus were analyzed to recognize the compositional differences depending on rice straw and waste cotton stuff media. Yields of add the rice bran 20% to the waste cotton stuff were increment 11% as compared with rice straw media. Crude protein of products to the waste cotton stuff media on Pleurotus ostreatus was a much and crude ash was less than of rice straw media. Glutamic acid was most and cystine was lowest among the amino acids of Pleurotus ostreatus. Total amino acids and essential amino acids of waste cotton stuff were much than of rice straw media.

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Quantitative Analysis of Total Aimno Acids and Free Sugars in Lycii fructus (진도산(珍島産) 구기자(枸杞子)의 아미노산조성(組成)과 유리당(遊離糖)의 분석(分析))

  • Lee, Mung-Yul;Sheo, Hwa-Joong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.3
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    • pp.249-252
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    • 1986
  • This quantitative analysis was carried out in order to investigate the isolation and identification of total amino acids and free sugars in Lycii fructus by using amino acid autoanalyzer and HPLC. 17 kinds of amino acids were analyzed by amino acid autoanalyzer, that is, aspartic acid, proline, glyclne, analine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenyla lanine, histidine, lysine, arginine, threonine, serine and glutamic acid. Threonine (3,745mg/100g) was the richest among them and total amounts of the essential amino acid, which was 53.93% of total amino acids, was 10,773mg/100g. Free sugars isolated by HPLC wet·e glucose, fructose and saccharose.

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