• 한국수정란이식학회지
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• 제1권1호
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• pp.35-49
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• 1986

Recipients are an integral part of embryo transfer and they are expensive to maintain as a good recipient. Recipient management is one of the most important components in a successful embryo transfer program. Management includes selection and subsequent care of the animals. A good recipient is basically on "open" cows or heffers whose reproductive tract is capable of receiving one or two embryos and incubating it to term. Potential recipients should be always be healthy and cycling normally ranging from 18 to 23 days. A thorough veterinary examination is recommended for candidate of recipients and cattle for questionable health should be eliminated from the recipient herd. Age and size of recipients are particularly important considerations when heifers are used, because of most embryos available for transfer are from large dams and sires. Body condition can influence a recipient's production, reproduction and health. Obese and underconditioned cattle should be avoided for use. Transfer of fresh embryos especially requires precise synchronization of donors and recipients. For estrus synchronization, PGF$_2$$\alpha$ is injected twice 10 to 12 days apart and short4erm progestagen treatment is applied to potential recipient cattle by coil into vagina (PRID) or ear implant (Synchro-Mate-B). The highest pregnancy results are achieved in recipients at exact synchrony with donors or 12 to 24 hr earlier than donors. Estrus detection is a major factor in breeding efficiency. High accuracy can be achieved by use of heat mount detection alds or by obserbing cattle for 30-minute peroids 3 times daily. Assay progesterone in milk can be used to discrIminate between pregnant and nonprenant recipients. Rectal palpation on day 35 to 70 after is an accurate and safe method of pregnancy diagnosis. Embryonic mortality in recipients may be associated with factors such as high environmental temperature and nutritional or lactational stress in early lactation period. Achievement of short calving interval requires concentrated management activity during the first 90 days following calving. Acceptable candidate for a recipient should be routinely vaccinated for infectious diseases. Proper nutritional programs according to NRC requirements and body condition scoring system for recipient cattles are vital to the ultimate success of an embryo transfer program.r program.

• 미생물학회지
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• 제43권1호
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• pp.1-6
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• 2007

Epstein-Barr virus (EBV), cytomegalovirus (CMV), hepatitis B virus (HBV), parvovirus B19 (B19)등 4종의 바이러스는 인체에 감염을 일으키는 병원체로서 DNA를 유전물질로 함유한다. 각 바이러스 유전자의 염기서열을 분석하여 EBV CMV, HBV의 pol 유전자와 B19의 ns 유전자에 특이적으로 결합할 수 있는 primer를 설계 제작하고 단일 시험으로 4종의 바이러스를 동시에 검출할 수 있는 다중 중합효소 연쇄반응(Multiplex PCR)법을 확립하였다. Primer 염기서열, PCR 반응조성물의 농도, PCR 반응시간 및 온도조건을 최적화하여 민감도를 증대시킴으로써, 단일 시험으로 5-10 분자수의 유전물질까지 검출이 가능하였다. 또한 4종의 바이러스 사이에 교차반응이 일어나지 않았으며 생체시료를 이용한 시험에서도 특이성과 민감도가 유지됨을 확인하였다. 그러므로 본 연구에서 확립한 다중 중합효소 연쇄반응은 세포배양액 또는 생체 시료에 감염된 4종 DNA 바이러스진단에 효율적으로 이용할 수 있을 것이라고 판단된다.

• 한국해양환경ㆍ에너지학회지
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• 제3권3호
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• pp.50-57
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• 2000

낙동강 하구해역에서의 물질순환과 영양염 flux의 특성을 파악하기 위해 단순박스 모델을 이용하여 담수수지, 염분수지, DIN과 DIP의 물질수지를 산정하였다. 하구와 인근해역의 염분으로 산정한 수지에 의해, 모델영역으로 유입되는 담수의 양은 0.94×10/sup 9/m³/month 였고, 해수에 의한 교환량은 2.7×10/sup 9/m³/month였으며, 담수의 체류시간은 2.03 day으로 나타났다. 영양염의 부하량은 DIN과 DIP가 각각 3.7×10³ton/month, 69.4ton/month로 나타났다. 유입 DIN의 2.6%와 유입 DIP의 8.6%가 식물플랑크톤에 의해 섭취되고 있고, 장림하수처리장에서의 영양염 부하량은 모델영역으로 유입되는 질소부하량의 16%, 인부하량의 10.2%를 차지하고 있다.

• 대한안전경영과학회지
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• 제12권1호
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• pp.51-57
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• 2010

Cycling that transform human energy into mechanical energy is one of the man-machine systems out of sports fields. Benchmarking means "improving ourselves by learning from others', therefore benchmarking toward dominant cyclist is necessary on field. The goals of this study were to provide important factors on multi-disciplines (kinematics, physiology, power, psychology) for a tailored-training program that is suitable to individual characteristics. Two cyclists participated in this study and gave consent to the experimental procedure. One was dominant cyclist (years: 21 yrs, height: 177 cm, mass: 70 kg), and the other was non-dominant cyclist (years: 21, height: 176, mass: 70). Kinematic data were recorded using six infrared cameras (240Hz) and QTM (software). Physiological data (VO2max, AT) were acquired according to graded exercising test with cycle ergometer and power with Wingate test used by Bar-Or et. al (1977) and to evaluate muscle function with Cybex. Psychological data were collected with competitive state anxiety inventory (CSAI-2) that was devised by Martens et. al (1990) and athletes' self-management questionnaire (ASMQ) of Huh (2003). It appears that the dominant's CV of ankle joint angle was higher than non-dominant's CV and dominant's pedaling pattern was consistent in biomechanics domain, which the dominant's values for all factors ware higher than non-dominant's values in physical, and physiological domain, and their values between cognitive anxiety and somatic anxiety were contrary to each other in psychology. Further research on multi-disciplines may lead to the development of tailored-optimal training programs applicable with key factors to enhance athletic performance by means of research including athlete, coach and parents.

• Corrosion Science and Technology
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• 제14권4호
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• pp.177-183
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• 2015

Prestressed Concrete steel Cylinder Pipe (PCCP) is extensively used as seawater pipes for cooling in nuclear power plants. The internal surface of PCCP is exposed to seawater, while the external surface is in direct contact with underground soil. Therefore, materials and strategies that would reduce the corrosion of its cylindrical steel body and external steel wiring need to be employed. To prevent against the failure of PCCP, operators provided a cathodic protection to the pre-stressing wires. The efficiency of cathodic protection is governed by the anodic performance of the system. A mixed metal oxide (MMO) electrode was developed to meet criteria of low over potential and high corrosion resistance. Increasing coating cycles improved the performance of the anode, but cycling should be minimized due to high materials cost. In this work, the effects of $RuCl_3$ concentration on the electrochemical properties and lifespan of MMO anode were evaluated. With increasing concentration of $RuCl_3$, the oxygen evolution potential lowered and polarization resistance were also reduced but demonstrated an increase in passive current density and oxygen evolution current density. To improve the electrochemical properties of the MMO anode, $RuCl_3$ concentration was increased. As a result, the number of required coating cycles were reduced substantially and the MMO anode achieved an excellent lifespan of over 80 years. Thus, we concluded that the relationship between $RuCl_3$ concentration and coating cycles can be summarized as follows: No. of coating cycle = 0.48*[$RuCl_3$ concentration, $M]^{-0.97}$.

• 콘크리트학회논문집
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• 제28권4호
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• pp.395-405
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• 2016

이 연구에서는 태양열 에너지 저장용도로 사용하기 위한 섬유보강 모르타르의 열적 및 역학적 특성을 파악하였다. 다양한 시멘트 복합재료의 배합이 섬유보강 모르타르의 열적 및 역학적 특성에 미치는 영향을 파악하기 위한 실험연구를 수행하였다. 섬유보강 모르타르의 역학적 특성으로서 열싸이클 전과 후의 압축강도 및 인장강도를 측정하였다. 또한, 섬유보강 모르타르의 열적 특성으로서 열전도율과 비열을 측정하였다. OPC와 그라파이트를 포함한 배합의 잔류압축강도가 가장 크게 나타난다. 알루미나 시멘트를 혼합한 배합의 비열이 크게 나타나며, 이는 알루미나시멘트가 열저장 시스템의 효율적인 축열과 방열에 유리함을 의미한다. 또한, 그라파이트의 첨가는 섬유보강 복합재료의 비열을 증가시킨다. 실험연구결과는 콘크리트를 $450^{\circ}C$ 이상의 열저장 매체로 활용하기 위한 프로토타입 시스템 설계에 실제적인 기초자료로 활용될 수 있을 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권4호
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• pp.1395-1399
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• 2012

Background: Histone deacetylase (HDAC) inhibitors have been reported to induce cell growth arrest, apoptosis and differentiation of tumor cells. The present study aimed to examine the effects of trichostatin A (TSA), one such inhibitor, on the cell cycle, apoptosis and invasiveness of osteosarcoma cells. Methods: MG-63 cells were treated with TSA at various concentrations. Then, cell growth and apoptosis were determined by 3-(4, 5-dimethyl-2-thiazolyl)-2H-tetrazolium bromide (MTT) and TUNEL assays, respectively; cell cycling was assessed by flow cytometry; invasion assays were performed with the transwell Boyden Chamber system. Results: MTT assays revealed that TSA significantly inhibited the growth of MG-63 cells in a concentration and time dependent manner. TSA treated cells demonstrated morphological changes indicative of apoptosis and TUNEL assays revealed increased apoptosis of MG-63 cells after TSA treatment. Flow cytometry showed that TSA arrested the cell cycle in G1/G2 phase and annexin V positive apoptotic cells increased markedly. In addition, the invasiveness of MG-63 cells was inhibited by TSA in a concentration dependent manner. Conclusion: Our findings demonstrate that TSA inhibits the proliferation, induces apoptosis and inhibits invasiveness of osteosarcoma cells in vitro. HDAC inhibitors may thus have promise to become new therapeutic agents against osteosarcoma.

• 대한화학회지
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• 제50권3호
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• pp.243-246
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• 2006

박막은 Pt/Ti/SiO2/Si 기판 위에 구연산 졸을 이용하여 spin coating에 의해 제작하였다. 기판위에 코팅된 구연산 졸을 380oC에서 15분간 건조시킨 후 750oC에서 10분간 열처리하여 박막을 얻었다. 얻어진 박막은 X-선 회절분석 결과 R3m의 결정구조를 가짐을 알수 있었고, 전기화학적 특성의 측정결과 1차 방전용량은 0.35Ah/cm2-m로 측정되었다.

• 한국세라믹학회지
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• 제34권1호
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• pp.88-94
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• 1997

플라즈마 용사법(plasma spray method)으로 제작된 상용 가스 터빈 연소기의 finned segment의 열차폐용 코팅계, ZrO2-8wt%Y2O3 top coat/Ni-26Cr-5Al-0.5Y bond coat/Hastelloy X superalloy 기판에서 NiCrAlY bond coat의 산화 거동과 열피로 파괴에 대하여 조사하였다. 생성된 bond coat의 주산화물은 NiO, CrO2, Al2O3였다. ZrO2/bond coat계면에서 생성된 산화물의 분포는 고온에서의 사용 전에 이 계면 아래에 얇은 층의 Al2O3가없는 곳에서는 NiO 산화층 및에 Cr2O3와 Al2O3가 혼합된 형태를 나타내었다. 열피로에 의해 박리된 시편의 파면을 관찰한 결과, 파괴는 주로 ZrO2/산화층 계면보다 세라믹층내로 약간 치우쳐서 일어나지만, 산화층 내에서도 약간 일어남을 알 수 있었다.

• JSTS:Journal of Semiconductor Technology and Science
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• 제16권6호
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• pp.760-770
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• 2016

A 12-bit 750 kS/s Dual-Sampling Successive Approximation Register Analog-to-Digital Converter (SAR ADC) technique with reduced Capacitive DAC (CDAC) is presented in this paper. By adopting the Adaptive Power Control (APC) technique for the two-stage latched type comparator and using bootstrap switch, power consumption can be reduced and overall system efficiency can be optimized. Bootstrapped switches also are used to enhance the sampling linearity at a high input frequency. The proposed SAR ADC reduces the average switching energy compared with conventional SAR ADC by adopting reduced the Most Significant Bit (MSB) cycling step with Dual-Sampling of the analog signal. This technique holds the signal at both comparator input asymmetrically in sample mode. Therefore, the MSB can be calculated without consuming any switching energy. The prototype SAR ADC was implemented in $0.18-{\mu}m$ CMOS technology and occupies $0.728mm^2$. The measurement results show the proposed ADC achieves an Effective Number-of-Bits (ENOB) of 10.73 at a sampling frequency of 750 kS/s and clock frequency of 25 MHz. It consumes only 0.13 mW from a 5.0-V supply and achieves the INL and DNL of +2.78/-2.45 LSB and +0.36/-0.73 LSB respectively, SINAD of 66.35 dB, and a Figures-of-Merit (FoM) of a 102 fJ/conversion-step.