• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.202-202
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• 2017

Global climate change resulted in unwarranted change in global temperature and caused heat and cold stress, which are consider major threat to agriculture productivity around the world. The use of plant growth-promoting microbes is an eco-friendly strategy to counteract such stresses and confer tolerance to the plants. In current study, previously isolated endophytic fungi Preussia sp. BSL-10 has been found to produce phytohormones such as IAA and GA and as such, endophyte Preussia sp. BSL-10 found to induced tolerance against heat and cold stress. The results showed that under both heat and cold stress the plant growth parameter such as shoot, root length, shoot fresh weight and root fresh weight is higher in Preussia sp. BSL-10 treated plants as compare to free Preussia sp. BSL-10 control plants. In addition, the stress-sensitive endogenous ABA levels were significantly increased in Preussia sp. BSL-10 host plant. The current result suggest that the phytohormone-producing endophyte Preussia sp. BSL-10 can increase plant resistance toheat and cold stress, in turn improving agricultural productivity.

• Journal of Microbiology and Biotechnology
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• 제20권12호
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• pp.1744-1749
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• 2010

The role of endophytic fungi in plant growth and development is well documented. However, endophytic fungi with growth promotion capacity have never been isolated from weeds previously. In the current study, we isolated 8 fungal endophytes from the roots of Monochoria vaginalis, a serious weed of rice paddy in Korea. These isolates were screened on Waito-C, in order to identify plant growth promoting metabolites. Two fungal isolates (M5.A & M1.5) significantly promoted the plant height and shoot length of Waito-C during preliminary screening experiments. The culture filtrates (CFs) of M5.A and M1.5 also promoted the shoot length of Echinocloa crusgalli. Gibberellins (GAs) analysis of the CFs of M5.A and M1.5 showed that these endophytic fungi secrete higher quantities of GAs as compared with wild-type G. fujikuroi KCCM12329. The CF of M5.A contained bioactive GAs ($GA_3$, 2.8 ng/ml; $GA_4$, 2.6 ng/ml, and $GA_7$, 6.68 ng/ml) in conjunction with physiologically inactive $GA_9$ (1.61 ng/ml) and $GA_{24}$ (0.18 ng/ml). The CF of M1.5 contained physiologically active GAs ($GA_3$, 1.64 ng/ml; $GA_4$, 1.37 ng/ml and $GA_7$, 6.29 ng/ml) in conjunction with physiologically inactive $GA_9$ (3.44 ng/ml), $GA_{12}$ (0.3 ng/ml), and $GA_{24}$ (0.59 ng/ml). M5.A and M1.5 were identified as new strains of Penicillium sp. and Aspergillus sp., respectively, based on their 18S rDNA sequence homology and phylogenetic analysis.

• 농업과학연구
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• 제37권2호
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• pp.171-175
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• 2010

This study was carried out to find the effect on growing media and proper time for water supply in rice seedling stages. Plant height of rice seedling for SIPAN-BRL among current several growing medias was higher in Chuchungbyeo than Ilpumbyeo, and that of root length was higher in check among used growing medias. Fresh and dry weight of shoot and root of two rice cultivar. Water time to absorption on each treatment of growing media concentration took similarly in SIPAN-BNH and check were similar. But 500 minutes did in 100ppm and did 370 and 470minute in 300 and 500ppm, respectively. Wilting time of rice seedling was appeared highly at 300ppm of SIPAN-BRL in Chuchungbyeo and 100ppm of SIPAN-BRL in Ilpumbyeo, while it prolonged at 300ppm in Chuchungbyeo and 500ppm in Ilpumbyeo, respectively. Water content at wilting stage was appeared highly at SIPAN-BRL 500ppm in both Chuchungbyo and Ilpumbyeo, while SIPAN-BNL was high in 300ppm of Chuchung-byeo and Ilpumbyeo, respectively. Plant and root length of SIPAN-BNL treated with 300ppm in 10day-rice seedling were highly appeared, while dry weight of shoot was high at 300ppm SIPAN-BNL.

• 한국육종학회지
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• 제40권1호
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• pp.15-21
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• 2008

It was previously pointed out that mutation is the ultimate source of variation. Adequate variation is needed for plant breeding if there is a limitation in natural genetic resources. When the ionizing radiation has been known to cause chromosomal and genomic alternations, it is widely used for inducing mutagenesis. The electron beam as an ionizing radiation is the principal physical mutagens that induces mutation and effectively used in plant breeding. Since dose-response relationships of electron beam in plant species are rarely known, we investigated the seed germination rate and early seedling growth of irradiated seeds of creeping bentgrass (Agrostis palustris Huds., cv Penn-A1) with various electron beam irradiating conditions (1, 1.3, 2 MeV at both 0.03 mA and 0.06 mA with dose of 100 Gy (Gray) and 0.03, 1, 1.3, 2 MeV at 0.03 mA with dose of 200 Gy, respectively) using electron accelerator at Korea Atomic Energy Research Institute. The growth parameters in terms of shoot length, primary root length, and secondary root length showed similar response between 0.06 / 1 (mA / MeV) at 100 Gy and 0.03 / 0.3 (mA / MeV) at 200 Gy. Bentgrass seed germination was mainly affected by the intensity of irradiated dose (Gray). Germination rate was lowered as the irradiated dose increased. On the other hand, early seedling growth was mainly governed not by the dose of radiation but by voltage.

• 환경생물
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• 제21권4호
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• pp.400-404
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• 2003

This study was to evaluate the effects of gamma irradiation on the germination, nutrient concentrations and growth of spinach and radish. Both the spinach and radish seeds exhibited relatively higher germination rates in response to the low doses of gamma irradiation compared to the non -irradiated control. Leaf DW of the radish did not respond to gamma irradiation but that of the spinach increased significantly in response to a gamma radiation of 4 Gy (P< 0.05). Leaf growth parameters of the spinach including the leaf area and SLA (leaf area/leaf dry weight) also demonstrated increased responses to gamma irradiation. R/S (root dry weight/shoot dry weight), root DW and root length of the spinach exhibited a positive response to gamma irradiation while those of the radish did not. In contrast, SRL (root length/root dry weight) significantly decreased with gamma irradiation at 8 Gy for the spinach, but not for the radish. The tissue nitrogen concentrations of the spinach showed an increased response to gamma irradiation while that of the radish did not. Furthermore, higher concentrations of phosphorus, potassium, calcium and magnesium were found in the irradiated spinach, but not in the irradiated radish. It seems that the non-specific physiological and/or biochemical activities of spinach might be accelerated by gamma irradiation, possibly accounting for the stimulation of nutrient uptake from the root media and early biomass accumulation in the current study.

• 한국유기농업학회지
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• 제25권1호
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• pp.125-134
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• 2017

본 연구는 친환경 유기과원의 부란병 방제를 위한 유황토분의 효과를 검증코자 2년간 수행되었다. 제조된 유황토분의 사과 부란병 치유효과를 검증하기 위해 유기과원에는 석회유황합제의 처리와 비교하였으며, 관행과원에서는 석회유황합제, 유황토분 및 네오아소진을 처리하였다. 유기과원의 유황토분의 부란병 치유율은 대조구에 비해 뚜렷하게 유의성이 인정되었으나, 2013년 봉현 과원을 제외하고는 석회유황합제와는 큰 차이가 없었다. 유기과원의 과실품질조사 결과, 과피의 붉은 색은 대조구에 비해 석회유황합제 처리구에서 다소 높게 나타냈으며, 과실의 경도에서는 유황토분 처리구가 대조구에 비해 유의적으로 높게 나타났다. 관행과원에서의 유황토분, 석회유황합제 및 네오아소진 처리는 대조구에 비해 통계적 유의적으로 높게 조사되었다. 유황토분의 부란병 치유율은 유기사과과원 및 관행사과과원에서 87.5~97.5%인 것으로 조사되었다. 관행과원에서의 유황토분 처리는 신초생육에 있어 네오아소진에 비해 통계적 유의성이 있게 증가하였다. 과실품질의 경우 유황토분처리는 과실의 붉은 색의 증가를 보였으나, 다른 과실품질인 무게, 경도, 당도 및 적정산도에서 통계적 유의성을 나타내지 않았다.

• Current Research on Agriculture and Life Sciences
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• 제5권
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• pp.1-11
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• 1987

벼상자육묘(箱子育苗)에서 살균제(殺菌齊)와 생장조절제처리(生長調節劑處理)가 묘생육(苗生育) 및 생리장해(生理障害)에 미치는 영향(影響)을 구명(究明)코자 삼강(三綱)벼와 낙동(洛東)벼를 공시(供試)하여 시험(試驗)하였던 결과(結果)를 요약(要約)하면 다음과 같다. 살균제처리(殺菌齊處理)에서 삼강(三綱)벼는 Metalaxyl과 SF8002 처리구(處理區)가, 낙동(洛東)벼는 SF8002와 Metalaxyl처리구(處理區)가 초장(草長)과 3엽(葉) 및 4엽(葉)이 뚜렷한 신장교과(伸長效果)를 보였다. 건물중(乾物重)은 Metalaxyl구(區)가 가장 무거웠고 엽수(葉數)와 묘충실도(苗充實度)는 Dachigaren청리구(廳理區)가 양호(良好)하였으며, 살균제처리구(殺菌齊處理區)가 무처리(無處理)에 비(比)해 입고병발생(立枯病發生)이 적었다. 생장조절제처리구(生長調節劑處理區)에서 삼강(三綱)벼에서는 BA처리구(處理區)가, 낙동(洛東)벼에서는 GA，처리구(處理區)가 초장(草長)이 가장 길었으며, ABA(處理區)가 초장(草長)이 가장 작았고, ABA와 CCC처리구(處理區)가 신근발근력(新根發根力)이 왕성(旺盛)했다. 살균제(殺菌齊)+생장조절제처리구(生長調節劑處理區)에서 살균제(殺菌齊)+GA, 구(區)와 Metalaxyl+생장조절제처리구(生長調節劑處理區)가 초장(草長)이 가장 길었으며, Isoprothiolane+생장조절제처리구(生長調節劑處理區)가 초장(草長)이 가장 짧았다. Dachigaren+IAA 처리구(處理區)가 건물중(乾物重)과 묘충실도(苗充實度)가 양호(良好)하여 묘소질(苗素質)이 좋았으며, 살균제(殺菌齊)+생장조절제처리구(生長調節劑處理區)가 무처리(無處理)보다 입고병발생(立枯病發生)이 적었다. 또한 삼강(三綱)벼에서는 Metalaxyl+IAA 처리구(處理區)가 낙동(洛東)벼에서는 Dachigaren+IAA 처리구(處理區)가 지상부재생력(地上部再生力)이 양호(良好)했다.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.202-207
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• 2010

Plant growth promoting fungi (PGPF) are well known for the production of useful secondary metabolites. However, limited information is available on the gibberellin (GA) production capacity of PGPF of endophytic origin. In the current study, 15 fungal endophytes were isolated from the roots of Crown daisy, and then screened on Waito-c rice, in order to identify plant growth promoting fungi. The fungal isolate MH7 significantly increased the shoot length (12.1 cm) of Waito-c in comparison with control treatment (7.9 cm). In a separate experiment, the culture filtrate (CF) of MH7 significantly promoted the growth attributes of Crown daisy. The MH7 CF was analyzed for gibberellins and it contained all physiologically active gibberellins ($GA_1$, 1.37 ng/ml; $GA_3$, 5.88 ng/ml; $GA_4$, 8.62 ng/ml; and $GA_7$, 2.05 ng/ml) in conjunction with physiologically inactive $GA_9$ (0.83 ng/ml), $GA_{12}$ (0.44 ng/ml), $GA_{15}$ (0.74 ng/ml), $GA_{19}$ (1.16 ng/ml), and $GA_{20}$ (0.98 ng/ml). The CF of MH7 produced higher amounts of $GA_3$, $GA_4$, $GA_7$, $GA_9$, and $GA_{12}$ than wild-type Fusarium fujikuroi, which was used as a control for GA production. The fungal isolate MH7 was later identified as a new strain of Penicillium on the basis of its morphological characteristics and phylogenetic analysis of the 188 rDNA sequence.

• Current Research on Agriculture and Life Sciences
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• 제15권
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• pp.101-107
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• 1997

미생물 제재인 Bioenzyme은 호기성 조건하에서 총 세균수는 $4.0{\times}10^6{\sim}9.6{\times}10^6CFU/g$으로 존재하였고 혐기적 조건하에서는 $1.5{\times}10^6{\sim}1.27{\times}10^7CFU/g$으로 존재하였으며 2종의 우점종이 확인 되었다. PN-1은 생체 균수가 $5.1{\times}10^6CFU/g$으로 분포율이 약 75% 였으며 PN-2균주가 $1.0{\times}10^6CFU/g$으로 약 15%내외를 차지하였으며, 우점종으로 확인된 균주의 동정결과 PN-1은 Bacillus licheniformis로 확인되었으며, PN-2는 Bacillus thuringiensis로 확인되었다. 미생물 제재인 Bioenzyme을 처리하였을 경우 상추의 수량에 미치는 영향은 생체 중의 경우 1, 2차 시험시 공히 5% 수준의 유의차가 인정되었으며, 엽수의 경우 유의차가 인정되지 않았다. 초장 및 엽폭의 경우 관행의 N,P,K구와 N,P,K+bioenzyme구사이에 1차시험에서는 유의차가 인정되었으나 2차시험에서는 유의차가 인정되지 않았다.

• 한국토양비료학회지
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• 제30권4호
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• pp.377-383
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• 1997

제약폐기은행잎의 유기물자원화 및 식물생장억제제 개발을 위한 기초자료로 활용코자 은행잎 제약폐기물중에 함유된 식물생육 억제물질을 분리 동정하고 이들물질을 처리하여 무와 벼의 발아 및 초기생육을 검토한 결과는 다음과 같다. 1) 폐기은행잎 조추출물 10% 용액은 무 종자 발아율을 저하시키고 유묘의 생육을 억제하였으나, 1% 농도에서는 뿌리 길이가 약간 짧아졌고, 0.1% 농도는 무처리와 비슷하였다. 벼 발아율은 조추출물 10% 농도에서 31%이상 억제되었다. 2) 지베렐린 $A_3(3.3{\times}10^{-6}M)$와 조추출액(10%)을 벼 유묘와 함께 처리하면 지베렐린 $A_3(3.3{\times}10^{-6}M)$ 단독처리보다 제2엽초장이 짧았다. 3) 조추출액의 산도를 달리한 3종의 액액분리물은 (EtOAc $(pH9)A_1$, EtOAc $(pH3)A_2$, BuOH $A_3$) 모두 1% 농도에서 무 종자의 발아를 완전히 억제하였다. 4) 조추출액을 액액분리, column chromatography, HPLC에 의한 분리정제를 통하여 활성성분을 분리하고, GC/MS, NMR 분석 결과 phenol류인 catechol로 판명되었으며, 폐기은행잎 29kg으로부터 분리한 catechol량은 0.76g이었다.