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Preventive Effects of Whole Grain Cereals on Sarcopenic Obesity in High-fat Diet-induced Obese Mice (고지방식이 동물모델에서 통곡물 시리얼의 근감소성 비만 예방 효과)

  • Kim, Mi-Bo;Lee, Sein;Kim, Changhee;Hwang, Jae-Kwan
    • Food Engineering Progress
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    • v.22 no.4
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    • pp.358-365
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    • 2018
  • Whole grain cereal (WGC)-rich diets provide macronutrients that are important for the regulation of energy metabolism. The current study evaluated whether WGCs had a preventive effect on sarcopenic obesity in high-fat diet (HFD)-induced obese mice. C57BL/6N mice were fed a normal diet (ND), ND+WGC, HFD, and HFD+WGC for 12 weeks. WGCs significantly reduced body weight gain, food efficiency ratio, fat mass, and adipocyte size in HFD-induced obese mice. WGCs attenuated HFD-induced nonalcoholic fatty liver disease by decreasing liver weight and hepatic fat accumulation. In addition, WGCs increased muscle strength and muscle mass in HFD-induced obese mice as well as in ND mice. Taken together, WGCs can be employed as functional food materials for the prevention of sarcopenic obesity by inhibiting fat accumulation and increasing muscle mass.

Influence of spent ginger yeast cultures on the production performance, egg quality, serum composition, and intestinal microbiota of laying hens

  • Liu, Junhan;Jin, Yuhong;Yang, Junhua
    • Animal Bioscience
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    • v.35 no.8
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    • pp.1205-1214
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    • 2022
  • Objective: Spent ginger is a byproduct of juice extraction from the rhizome of ginger (Zingiber officinale). Despite its nutritional value, it is difficult to preserve or further process and thus is often wasted. This study uses spent ginger as a substrate for fermentation and cultivates spent ginger yeast cultures (SGYCs) that are then added to the feed of laying hens. The effects of SGYCs on production performance, egg quality, serum composition, and intestinal microbiota of laying hens were investigated. Methods: Eighty 60-week-old Hy-Line Brown hens were separated into 5 experimental groups with 4 replicates per group (4 hens per cage, 4 cages per replicate). The control group was fed a basal diet while experimental groups were also given SGYCs at the levels of 5, 10, 20, and 40 g/kg for 6 weeks. Results: The addition of SGYCs significantly increased the laying rate and nutrient digestibility, decreased feed conversion ratio, and enhanced the color of egg yolks (p<0.05). No changes were observed in activity levels of alanine aminotransferase and aspartate aminotransferase in the serum (p>0.05), but the activities of superoxide dismutase, glutathione peroxidase, and peroxidase all significantly increased, and contents of malondialdehyde were significantly reduced (p<0.05). In addition, changes in the relative abundance of Firmicutes and Bacteroidetes might be the main factor contributing to the significant increase in the apparent digestibility of crude protein and crude fat in laying hens (p<0.05). Conclusion: The current evidence shows that dietary supplementation of SGYCs to the feed of laying hens can improve laying rates, enhance antioxidative defenses, and influence dominant intestinal bacteria.

Evaluating productive performance, meat quality and oxidation products of Italian White breed rabbits under free-range and cage rearing system

  • Tufarelli, Vincenzo;Tateo, Alessandra;Schiavitto, Michele;Mazzei, Domenico;Calzaretti, Giovanna;Laudadio, Vito
    • Animal Bioscience
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    • v.35 no.6
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    • pp.884-891
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    • 2022
  • Objective: Free-range systems have been increasingly available to the consumer due to increased demand for more sustainable meat-products. In the current study, the effect of free-range (FR) and cage system (CS) was explored on growth performance, meat quality and oxidation products in Italian White breed rabbits during the growing-fattening phase (5 to 13 weeks of age). Methods: Forty rabbits were randomly allotted to two treatment groups according to the rearing system, and each treatment group was replicated five times with four subjects in each replicate (20 rabbits per treatment-group). All rabbits fed the same diet as pelleted, and under FR system, no additional feeds were available to animals. Results: Rearing system had significant effect on rabbit growth performance, where CS group resulted in higher final body weight (p<0.045) and gain (p<0.029) and better feed efficiency (p<0.025) compared to FR rabbits. Most carcass traits were not affected by rearing system; however, a reduction of abdominal fat content (p<0.015) and meat lipids (p<0.034) was observed in FR rabbits. Rearing system had no effects on meat fatty acid profile, whereas meat from FR rabbits resulted less susceptible to lipid and protein oxidation compared to caged animals. Conclusion: In overall, FR system could be suggested as a substitute for conventional caged system because of FR system preserved rabbit meat from oxidation.

A preliminary evaluation on mixed probiotics as an antimicrobial spraying agent in growing pig barn

  • Shanmugam, Sureshkumar;Jae Hong, Park;In Ho, Kim
    • Journal of Animal Science and Technology
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    • v.64 no.6
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    • pp.1035-1045
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    • 2022
  • The purpose of this study is to examine whether spraying an anti-microbial agent into the slurry pit will reduce the noxious odor substances from piggery barns. For this, a total of 200 crossbred ([Landrace × Yorkshire] × Duroc) growing pigs with an initial average body weight (BW) of 23.58 ± 1.47 kg were selected and housed in two different rooms, i.e. control (CON) and treatment (TRT). Each room has 100 pigs (60 gilts and 40 borrows). For a period of 42 days, all pigs were fed with corn-soybean meal-based basal diet. Later the noxious odor substances were measured by the following methods. First, fecal samples were randomly collected and stored in sealed and unsealed containers, and sprayed with the non-anti-microbial agent (NAMA) (saline water) and multi-bacterial spraying (MBS) agent (200 :1, mixing ratio-fecal sample : probiotic), Second, the slurry pit of CON and TRT rooms were directly sprayed with NAMA and MBS, respectively. The fecal sample that was stored in sealed and un-sealed containers and sprayed with MBS significantly reduced NH3 and CO2 concentration at the end of day 7. However, at the end of day 42, the fecal sample showed a lower H2S, methyl mercaptans, acetic acid, and CO2 concentration compared to the unsealed container. Moreover, at the end of days 7, 14, 21, 28, 35, and 42 compared to the CON room and TRT room slurry pit emits lower concentrations of NH3, acetic acid, H2S, and methyl mercaptans, and CO2 into the atmosphere. Based on the current findings, we infer that spraying anti-microbial agents on pig dung would be one of the better approaches to suppress the odor emission from the barn in the future.

Collaborative Modeling of Medical Image Segmentation Based on Blockchain Network

  • Yang Luo;Jing Peng;Hong Su;Tao Wu;Xi Wu
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.3
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    • pp.958-979
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    • 2023
  • Due to laws, regulations, privacy, etc., between 70-90 percent of providers do not share medical data, forming a "data island". It is essential to collaborate across multiple institutions without sharing patient data. Most existing methods adopt distributed learning and centralized federal architecture to solve this problem, but there are problems of resource heterogeneity and data heterogeneity in the practical application process. This paper proposes a collaborative deep learning modelling method based on the blockchain network. The training process uses encryption parameters to replace the original remote source data transmission to protect privacy. Hyperledger Fabric blockchain is adopted to realize that the parties are not restricted by the third-party authoritative verification end. To a certain extent, the distrust and single point of failure caused by the centralized system are avoided. The aggregation algorithm uses the FedProx algorithm to solve the problem of device heterogeneity and data heterogeneity. The experiments show that the maximum improvement of segmentation accuracy in the collaborative training mode proposed in this paper is 11.179% compared to local training. In the sequential training mode, the average accuracy improvement is greater than 7%. In the parallel training mode, the average accuracy improvement is greater than 8%. The experimental results show that the model proposed in this paper can solve the current problem of centralized modelling of multicenter data. In particular, it provides ideas to solve privacy protection and break "data silos", and protects all data.

Exploring the Combined Use of LiDAR and Augmented Reality for Enhanced Vertical and Horizontal Measurements of Structural Frames (골조 수직, 수평 측정작업 시 LiDAR 및 AR 기술 적용방안 제시)

  • Park, Inae;Kim, Sangyong
    • Journal of the Korea Institute of Building Construction
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    • v.23 no.3
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    • pp.273-284
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    • 2023
  • This study is centered on the combined use of LiDAR(Light Detection and Ranging) and AR(Augmented Reality) technologies during vertical and horizontal frame measurements in construction projects. The intention is to enhance the quality control procedure, elevate accuracy, and curtail manual labor along with time expenditure. Present methods for accuracy inspection in frame construction often grapple with reliability concerns due to subjective interpretation and the scope for human error. This research recommends the application of LiDAR and AR technologies to counter these issues and augment the efficiency of the inspection process, along with facilitating the dissemination of results. The suggested technique involves the collection of 3D point cloud data of the frame utilizing LiDAR and leveraging this data for checks on construction accuracy. Furthermore, the inspection outcomes are fed into a BIM (Building Information Modeling) model, and the results are visualized via AR. Upon juxtaposing this methodology with the current approach, it is evident that it offers benefits in terms of objective inspection, speed, precise result sharing, and potential enhancements to the overall quality and productivity of construction projects.

Improvement of the amplification gain for a propulsion drives of an electric vehicle with sensor voltage and mechanical speed control

  • Negadi, Karim;Boudiaf, Mohamed;Araria, Rabah;Hadji, Lazreg
    • Smart Structures and Systems
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    • v.29 no.5
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    • pp.661-675
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    • 2022
  • In this paper, an electric vehicle drives with efficient control and low cost hardware using four quadrant DC converter with Permanent Magnet Direct Current (PMDC) motor fed by DC boost converter is presented. The main idea of this work is to improve the energy efficiency of the conversion chain of an electric vehicle by inserting a boost converter between the battery and the four quadrant-DC motor chopper assembly. Consequently, this method makes it possible to maintain the amplification gain of the 4 quadrant chopper constant regardless of the battery voltage drop and even in the presence of a fault in the battery. One of the most important control problems is control under heavy uncertainty conditions. The higher order sliding mode control technique is introduced for the adjustment of DC bus voltage and mechanical motor speed. To implement the proposed approach in the automotive field, experimental tests were carried out. The performances obtained show the usefulness of this system for a better energy management of an electric vehicle and an ideal control under different operating conditions and constraints, mostly at nominal operation, in the presence of a load torque, when reversing the direction of rotation of the motor speed and even in case of battery chamber failure. The whole system has been tested experimentally and its performance has been analyzed.

Effects of dietary trace mineral levels on physiological responses, reproductive performance, litter performance, blood profiles, and milk composition in gestating sows

  • Hong Jun Kim;Xing Hao Jin;Sun Woo Kang;Yoo Yong Kim
    • Animal Bioscience
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    • v.36 no.12
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    • pp.1860-1868
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    • 2023
  • Objective: This study was conducted to evaluate the effects of optimal trace mineral levels on the physiological responses, reproductive performance, litter performance, blood profiles and milk composition in gestating sows. Methods: A total of 59 multiparous sows (Yorkshire×Landrace) with similar body weight (BW), backfat thickness (BF), and parity were assigned to one of four treatments with 14 or 15 sows per treatment using a completely randomized design. The treatments were 100% (M1), 300% (M3), 600% (M6), and 900% (M9) of the National Research Council (NRC) Nutrient Requirements of Swine. During lactation period, all the sows were fed the same commercial lactation diet. Results: No significant differences were observed in the BW, BF, reproductive performance, milk composition, or growth performance of the piglets. On day 70 of gestation, the serum zinc concentration showed a quadratic response to M6 treatment (quadratic, p<0.05). Moreover, as the dietary mineral levels increased, the zinc concentration increased linearly at 110 days of gestation (linear, p<0.05). Furthermore, copper and iron concentrations in the serum of sows at 24 h postpartum decreased linearly when high levels of dietary minerals were provided (linear, p<0.05). In the serum of piglets, serum zinc concentrations decreased linearly (linear, p<0.05), and iron concentration showed a quadratic response (quadratic, p<0.05) with an increase in trace mineral premix levels in gestation diets. Conclusion: The current trace mineral requirements of NRC (2012) are suitable for gestating sows, and the addition of dietary mineral levels in the gestating diet did not show any improvements during the gestation and lactation periods.

Effect of Silencing subolesin and enolase impairs gene expression, engorgement and reproduction in Haemaphysalis longicornis (Acari: Ixodidae) ticks

  • Md. Samiul Haque;Mohammad Saiful Islam;Myung-Jo You
    • Journal of Veterinary Science
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    • v.25 no.3
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    • pp.43.1-43.13
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    • 2024
  • Importance: Haemaphysalis longicornis is an obligate blood-sucking ectoparasite that has gained attention due its role of transmitting medically and veterinary significant pathogens and it is the most common tick species in Republic of Korea. The preferred strategy for controlling ticks is a multi-antigenic vaccination. Testing the efficiency of a combination antigen is a promising method for creating a tick vaccine. Objective: The aim of the current research was to analyze the role of subolesin and enolase in feeding and reproduction of H. longicornis by gene silencing. Methods: In this study, we used RNA interference to silence salivary enolase and subolesin in H. longicornis. Unfed female ticks injected with double-stranded RNA targeting subolesin and enolase were attached and fed normally on the rabbit's ear. Real-time polymerase chain reaction was used to confirm the extent of knockdown. Results: Ticks in the subolesin or enolase dsRNA groups showed knockdown rates of 80% and 60% respectively. Ticks in the combination dsRNA (subolesin and enolase) group showed an 80% knockdown. Knockdown of subolesin and enolase resulted in significant depletion in feeding, blood engorgement weight, attachment rate, and egg laying. Silencing of both resulted in a significant (p < 0.05) reduction in tick engorgement, egg laying, egg hatching (15%), and reproduction. Conclusions and Relevance: Our results suggest that subolesin and enolase are an exciting target for future tick control strategies.

Extracts of abalone intestine regulates fat metabolism in 3T3-L1 adipocytes and high fat diet-induced zebrafish larvae

  • Laxmi Sen Thakuri;Chul Min Park;Jin Yeong Choi;Hyeon-A Kim;Han Kyu Lim;Jin Woo Park;Dong Wook Kim;Dong Young Rhyu
    • Fisheries and Aquatic Sciences
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    • v.27 no.2
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    • pp.122-135
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    • 2024
  • Obesity is a disease involving mechanisms of fat accumulation, low-grade inflammatory cytokine release, and mitochondrial dysfunction. The aim of the current study was to investigate the effects of abalone intestine extract on fat metabolism in 3T3-L1 adipocytes and high fat diet-induced zebrafish larvae. The total phenol content was highest in subcritical water extract at 210℃ (SW210) among hot water, ethanol, and subcritical water extracts of abalone intestine. In addition, SW210 of male abalone intestine (MASW210) most effectively controlled the lipid accumulation and expression of adipogenic or lipogenic regulators (PPAR-γ, C/EBPα, SREBP-1c, and FAS) in 3T3-L1 adipocytes. Likewise, in zebrafish larvae fed high fat, MASW210 significantly suppressed body weight, glucose levels, and lipid accumulation. The mRNA expression related to adipogenesis (PPAR-γ and C/EBPα), lipogenesis (SREBP-1c and FAS), inflammatory cytokines (TNF-α and IL-6), energy m/;.etabolism (AMPK, lepr, SIRT1, and adiponectin), and mitochondrial biogenesis (PGC-1α and CPT-1) were significantly regulated by treatment with MASW210. These results suggest that abalone intestine extract such as MASW210, are useful biomaterials for improving obesity and metabolic diseases.