• Clinical and Experimental Reproductive Medicine
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• v.26 no.2
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• pp.137-148
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• 1999

Purpose of the present study was to find the optimal ovulation induction medicine for the maturation and development of immature oocytes and culture media for 2-cell embryos in the mouse model. ICR female mouse aged 6 to 8 weeks, were stimulated with 5 IU PMSG injection. At 47 to 50 hour post-PMSG injection, ovaries were dissected out and oocytes-cumulus complexes were punctured. The oocyte-cumulus complexes were cultured in media containing various ovulation induction medicine, CC, HMG and Metrodin for 18 hours. Female ICR mice were stimulated with 5 IU PMSG and 48 hours later were injected 5 IU of hCG, then female and male mice were mated. At 48 hour post-hCG injection, oviducts were dissected out and 2-cell embryos were flushed. The 2-cell embryos were cultured in various media, Ham's F-10 media of milli-Q water $(3^{\circ})$, Ham's F-10 media of HPLC (high performance liquid chromatography, Baxter) water, Medicult media, HTF (human tubal fluid) media for 96 hours. The results were as follows. 1. When the oocytes-cumulus complexes were cultured in $10^{-9}{\mu}g/ml{\sim}10^{-8}{\mu}g/ml$ of CC, those were suppressed in meiotic maturation $(28.2{\sim}33.7%)$. Whereas the oocytes-cumulus complexes were cultured in $10^{-7}{\mu}g/ml{\sim}10^{-4}{\mu}g/ml$, these were not effected in meiotic maturation $(54.5{\sim}72.7%)$. 2. When the oocytes-cumulus complexes were cultured in $10^{-4}{\mu}g/ml{\sim}10^{-1}{\mu}g/ml$ of Metrodin, those were suppressed in meiotic maturation $(35.7{\sim}41.5%)$. Meanwhile the oocytes-cumulus complexes were cultured in $10^{-7}{\mu}g/ml{\sim}10^{-5}{\mu}g/ml$, those were not effected in meiotic maturation $(54.2{\sim}70.3%)$. 3. When the oocytes-cumulus complexes were cultured in $10^{-5}{\mu}g/ml{\sim}10^{-4}{\mu}g/ml$ of HMG, those were suppressed in meiotic maturation $(48.2{\sim}50.4%)$. As being cultured in $10^{-7}{\mu}g/ml{\sim}10^{-6}{\mu}g/ml$, increased in meiotic maturation $(75.8{\sim}80.7%)$. 4. When the 2-cell embryos were cultured in Ham's F-10 media of milli-Q water $(3^{\circ})$, Ham's F-10 media of HPLC (high performance liquid chromatograpy, Baxter) water, Medicult media, HTF (human tubal fluid) media, developmental rates to blastocyst and hatching for 96 hour were 50.0%, 45.2%, 71.5% and 95.6%, respectively.

• 대한생식의학회:학술대회논문집
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• 1996.11a
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• pp.36-37
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• 1996

• 대한생식의학회:학술대회논문집
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• 1997.11a
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• pp.69-70
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• 1997

• 대한생식의학회:학술대회논문집
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• 2007.06a
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• pp.139-139
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• 2007

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2005.10a
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• pp.105-105
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• 2005

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2005.10a
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• pp.105-105
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• 2005

• Reproductive and Developmental Biology
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• v.35 no.4
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• pp.491-498
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• 2011

During mammalian fertilization, germ cell-specific hyaluronidases, such as sperm adhesion molecule 1 (SPAM1) and hyaluronoglucosaminidase 5 (Hyal5), are important for the dispersal of the cumulus mass. In this study, we demonstrated that bull Hyal5 is a single copy gene on chromosome 4 that is expressed specifically in the testis. In addition, we expressed recombinant bull SPAM1 and Hyal5 in human embryonic kidney 293T cells and showed that these enzymes possessed hyaluronidase activity. We also demonstrated that a polyclonal antibody against bull sperm hyaluronidase inhibits sperm-egg interactions in an in vitro fertilization (IVF) assay. Our results suggested that bull Hyal5 may have a critical role in bull fertilization.

• 대한생식의학회:학술대회논문집
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• 2001.11a
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• pp.88-88
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• 2001

• Atmosphere
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• v.21 no.4
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• pp.391-404
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• 2011

This study investigates the impact of cumulus parameterization scheme (CPS) with different horizontal grid sizes on the simulation of the local heavy rainfall case over the Korean Peninsula. The Weather Research and Forecasting (WRF)-based real-time forecast system of the Joint Center for High-impact Weather and Climate Research (JHWC) is used. Three CPSs are used for sensitivity experiments: the BMJ (Betts-Miller-Janjic), GD (Grell-Devenyi ensemble), and KF (Kain-Fritsch) CPSs. The heavy rainfall case selected in this study is characterized by low-level jet and low-level transport of warm and moist air. In 27-km simulations (DM1), simulated precipitation is overestimated in the experiment with BMJ scheme, and it is underestimated with GD scheme. The experiment with KF scheme shows well-developed precipitation cells in the southern and the central region of the Korean Peninsula, which are similar to the observations. All schemes show wet bias and cold bias in the lower troposphere. The simulated rainfall in 27-km horizontal resolution has influence on rainfall forecast in 9-km horizontal resolution, so the statements on 27-km horizontal resolution can be applied to 9-km horizontal resolution. In the sensitivity experiments of CPS for DM3 (3-km resolution), the experiment with BMJ scheme shows better heavy rainfall forecast than the other experiments. The experiments with CPS in 3-km horizontal resolution improve rainfall forecasts compared to the experiments without CPS, especially in rainfall distribution. The experiments with CPS show lower LCL(Lifted Condensation Level) than those without CPS at the maximum rainfall point, and weaker vertical velocity is simulated in the experiments with CPS compared to the experiments without CPS. It means that CPS suppresses convective instability and influences mainly convective rainfall. Consequently, heavy rainfall simulation with BMJ CPS is better than the other CPSs, and even in 3-km horizontal resolution, CPS should be applied to control convective instability. This conclusion can be generalized by conducting more experiments for a variety of cases over the Korean Peninsula.

• Journal of Korean Society for Atmospheric Environment
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• v.29 no.2
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• pp.135-151
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• 2013

This study has been carried out to analyze the sensitivity of ozone concentrations by employing different options of cumulus parameterization schemes (CPSs) and microphysics schemes in MM5 models. These sensitivity tests were applied to long-range transport case of higher ozone over Northeast Asia. Employed CPS schemes are Betts-Miller (BM), Grell (GR), Kain-Fritsch2 (KF2), Anthes-Kuo (AK), None scheme (grid scale physics only), and four microphysics used here are Simple ice, Reisner1, Reisner2, Schultz scheme in MM5. We chose two cases of high ozone long range transport case by employing both concentrations ozone level and backward trajectory model. The results showed that modeled ozone concentrations indicated about 10% differences among CPSs. Of the all options, GR and KF2 (for CPS), and Rersiner-1 and Resiner-2 (for microphysics) showed relatively good and stable variations against ensemble mean values. For both CPS and microphysics schemes, the difference of precipitation arising from different parameterization schemes was significant by itself, but the resultant ozone variations showed only marginal. But the cloud fraction differences arising from different parameterization schemes showed better correlation with ozone variations than precipitation differences, indicating that the photochemical ozone generation variations is more dominant by cloud fraction than wet removal process for high and long-ranged transported ozone cases over Northeast Asia.