• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7097-7103
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• 2014

Over-expression of de novo lipogenesis (DNL) genes is associated with the prognosis of various types of cancers. However, the effects of single nucleotide polymorphisms (SNPs) in these genes on recurrence and survival of non-small cell lung cancer (NSCLC) patients after surgery are still unknown. In this study, a total of 500 NSCLC patients who underwent surgery treatment were included. Eight SNPs in 3 genes (ACACA, FASN and ACLY) of the DNL pathway were examined using the Sequenom iPLEX genotyping system. Multivariate Cox proportional hazards regression and Kaplan-Meier curves were used to analyze the association of SNPs with patient survival and tumour recurrence. We found that two SNPs in the FASN gene were significantly associated with the recurrence of NSCLC. SNP rs4246444 had a significant association with lung cancer recurrence under additive model (hazard ratio [HR], 0.82; 95% confidence interval [95%CI], 0.67-1.00; p=0.05). Under the dominant model, rs4485435 exhibited a significant association with recurrence (HR, 0.75; 95%CI, 0.56-1.01; p=0.05). Additionally, SNP rs9912300 in ACLY gene was significantly associated with overall survival in lung cancer patients (HR, 1.41; 95%CI, 1.02-1.94, p=0.04) under the dominant model. Further cumulative effect analysis showed moderate dose-dependent effects of unfavorable SNPs on both survival and recurrence. Our data suggest that the SNPs in DNL genes may serve as independent prognostic markers for NSCLC patients after surgery.

• 한국환경농학회지
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• 제31권3호
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• pp.286-292
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• 2012

비타민A 강화벼와 낙동벼의 벼물바구미(Lissorhoptrus oryzophilus )에 대한 성충 전용살충제 Clothianidin 액상수화제의 살충제 감수성 시험을 실시한 결과, 비타민A 강화벼에서 72시간-$LC_{50}$은 유효농도 0.018mg/L (95% 신뢰한계는 0.013~0.023mg/L)이었으며, Bt벼의 모본으로 대조로 사용한 낙동벼의 72시간-$LC_{50}$은 유효농도 0.021mg/L(95% 신뢰한계는 0.016~0.026mg/L)이었다. 72시간-$LC_{50}$은 낙동벼에서 다소 높았지만, 해충저항성 Bt벼 72시간-$LC_{50}$이 낙동벼의 95% 신뢰한계 내에 포함되어, 두 품종의 $LC_{50}$값에 유의성이 없는 것으로 판단된다.

• 한국환경농학회:학술대회논문집
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• 한국환경농학회 2009년도 정기총회 및 국제심포지엄
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• pp.329-338
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• 2009

대기 중으로 휘산 되는 대부분의 암모니아는 농경지에 시용하는 가축분뇨, 퇴비 및 질소비료에서 유래하며, 휘산된 암모니아는 강하 (Deposition)를 통해서 자연생태계의 산성화를 초래하고 수계의 부영양화를 유발하는 원인물질로 작용한다. 본 연구에서는 돈분뇨 및 퇴비 시용방법에 따른 암모니아 휘산량을 정량적으로 평가하였다. 돈분퇴비 표층살포 (SA), 표층살포 후 즉시 경운(IRA), 표층살포 3일후 경운 (RA-3d) 처리구의 13일동안 암모니아 휘산량은 각각, 28.7, 8.7, 24.3 kg N/ha 로 IRA 처리구는 SA 처리구에 비해 약 70% 저감효과를 가져왔다. 돈분 액비 표층 살포 후 즉시 경운은 무경에 비해 봄과 가을 각각 26, 50% 의 암모니아 휘산량 저감 효과가 있었다. 그리고 수분 함량에 따른 암모니아 휘산량은 수분 함량이 높을수록 건조 토양에 비해 증가 하였다. 돈분 액비 시용 전후 담수 조건에 따른 암모니아 휘산량은 담수 상태에서 액비를 시용하는 것이 건토에 액비 살포 1일 후 담수 보다 약 4.7배의 암모니아 휘산량 증가가 있었다.

• 식물병연구
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• 제24권1호
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• pp.33-40
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• 2018

본 연구는 무기살균제, 식물추출물, 미생물 등 우리나라에 등록된 병해관리용 유기농자재 중 19종에 대해 배검은별무늬병균과 배붉은별무늬병균을 접종하고 배 잎 조직에서의 항균활성을 확인하고자 수행하였다. 배검은별무늬병균에 대해 황, 구리 등이 함유된 유기농자재 9종 중에서 대부분은 포자발아를 완전히 억제하였고, 일부 발아한 포자의 경우에도 부착기가 전혀 형성되지 않았으나 배붉은별무늬병균에 대해서는 석회황합제, 네오보르도, 흰가리스 등에 한하여 항균활성을 보였다. 식물추출물을 함유하는 유기농자재 중 흰가루자바는 배 검은 별무늬병균의 분생포자 발아를 완전하게 억제하였으며 배 붉은별무늬병균에 대해서도 71.6% 수준으로 비교적 높은 포자발아 억제효과를 보였다. 미생물 함유 제제로써 청고탄(Streptomyces griseus)은 배검은별무늬병균에 대해 88.8%의 포자발아억제율을 보이고 부착기도 형성하지 않으며 세포간에 자재집적이 확인되지 않았다. 탑시드(Paenibacillus polymyxa)는 배검은별무늬병균과 배 붉은별무늬병균에 대해 포자발아억제율이 각각 71.0%와 90.6% 수준이며 부착기형성도 이뤄지지 않았다. 향후 직접적인 접촉으로 인하여 발생되는 보호효과뿐만 아니라 미생물 대사산물과 천연화합물이 지니고 있는 유도저항성 효과도 고려하여 포장조건에서 누적적으로 살포하면서 종합적인 항균활성을 검토할 필요가 있다.

• 터널과지하공간
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• 제23권5호
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• pp.442-453
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• 2013

본 연구에서는 FLAC3D를 이용해 대용량 고온 열에너지저장소가 암반공동과 지상에 위치하는 경우를 각각 모델링하고 운영기간 5년 동안의 비정상상태해석을 수행하여 저장소 외벽을 통한 열손실을 비교 분석하였다. 두 저장모델의 운영 조건 및 입력물성은 모두 동일하나, 암반공동 열에너지저장소는 주변 암반의 전도 열전달에 의해서만 열손실이 발생하고, 지상 저장소는 대기의 대류 열전달에 의해서 열손실이 발생하는 것으로 가정하였다. 열에너지의 반복적인 주입과 토출에 따른 저장온도의 변화를 고려하여 수치해석모델을 작성하였으며, 단열재 두께에 따른 열손실 특성을 함께 검토하였다. 해석 결과, 지상식 저장시설은 운영 기간이 경과하더라도 일정한 열손실률을 보이는 반면 암반공동 저장시설의 열손실률은 운영 초기 단계에서 급격히 감소하여 일정한 값으로 수렴하는 경향을 보였다. 이러한 열손실의 감소는 시간 경과에 따라 주변 암반의 온도가 상승함으로써 저장소외벽에서의 열유속이 감소하기 때문으로 판단할 수 있다. 운영 후 5년 경과 시 암반공동 열에너지저장소의 누적열손실량은 지상저장소에 비해 약 72.7%로 나타났으며, 암반공동 저장시설의 열손실 특성은 주변 암반의 히팅 효과로 인해 지상식 저장시설에 비해 단열재 두께에 대한 민감도 및 의존도가 상대적으로 낮은 것으로 분석되었다.

• Weed & Turfgrass Science
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• 제7권2호
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• pp.148-157
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• 2018

본 연구는 외국산 피트를 포함해서 국내외 토양개량재 4종류가 크리핑 벤트그래스의 초기 활착 및 잔디밀도에 미치는 영향을 조사함으로 잔디밭 조성 시 실무에 활용할 수 있는 기초자료를 얻기 위해 수행하였다. 전체 20개 처리구는 식재층 모래에 유기질 토양개량재인 SABP (Berger peat), SAEP (Eco-peat), SAGS (G1-Soil) 및 SAPP (Premier peat)를 10-50% (v/v) 사이 혼합하여 준비하였다. 크리핑 벤트그래스의 초기 활착 및 밀도 변화는 토양개량재 종류에 따라 유의한 차이가 나타났다. 잔디밀도는 경시적인 변화가 나타나서 파종 2주 후 생육 초기 최저 36.7% (SABP 30)에서 최고 89.7% (SAGS 20)까지 처리구간 차이가 53% 정도 나타났다. 하지만 파종 6주 전후로 성숙기 밀도 수준에 도달해서 SABP 및 SAEP 일부 혼합구를 제외한 대부분 처리구에서 잔디밀도는 90% 전후로 비슷하였다. 실험 종료 시 최종 활착율은 최저 60.7% (SAEP 10)에서 최고 96.7% (SAPP 50)까지 처리구간 차이가 36.0%나 크게 나타났다. 잔디 활착율 및 밀도에 대한 최적 혼합율은 개량재 종류에 따라서 일정하지 않았다. SABP와 SAGS 개량재의 최적 혼합비율은 각각 10% 및 20%로 나타났지만, SAEP와 SAPP 개량재는 50%가 최적의 비율로 나타났다. 따라서 잔디밭 조성 전에 과학적인 분석을 통해 개별 토양개량재의 적정 혼합비율을 결정해서 실무에 적용하는 것이 필요하며, 또한 향후 주요 잔디 초종에서 외국산 및 국내산 토양개량재가 잔디생육에 미치는 종합적인 포장 적응성 실험을 통해 실무 응용에 활용하는 것이 바람직하다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권8호
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• pp.1118-1125
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• 2003

Research was carried out to clarify whether a suppression of dry forage intake during the early stages of feeding in ruminants is caused by feeding induced hypovolemia which is produced by the accelerated secretion of parotid saliva. Goats with a parotid fistula were fed roughly crushed alfalfa hay cubes, commercial ground concentrate feed and $NaHCO_3$ twice daily (10:00-12:00, 16:00-18:00). The animals were free access to drinking water all day prior to, during and after experiments. The animals were intraruminally infused every day prior to the morning feeding period with parotid saliva collected from the parotid fistula over a 24 h period. The present experiment consisted of two treatments, non-infusion (RNI) and intraruminal infusion of parotid saliva (RSF). In the RSF treatment, 4-5 kg of parotid saliva (280-290 mOsm/l) collected over a 24 h period was intraruminally infused 1 h prior to the commencement of the morning feeding. During feeding, eating and parotid saliva secretion rates were measured. Blood samples were also periodically collected from the jugular vein. During and after 2 h feeding, water intakes were measured, respectively. These measurements were used to define thirst levels. It is thought that rumen fill in the RSF treatment was higher than the RNI treatment. Plasma osmolality in the RSF treatment increased in the first half of the 2 h feeding period due to the intraruminal infusion of parotid saliva. Therefore, parotid saliva secretion rates in the RSF treatment were lower than the RNI treatment for 30 min period from 30 to 60 min after the commencement of feeding. On the other hand, plasma total protein concentration and hematocrit in the RSF treatment decreased by 3.2 and 3.3% prior to the commencement of feeding due to the intraruminal infusion of parotid saliva. In the first half of the 2 h feeding period, plasma total protein concentration and hematocrit in the RSF treatment showed a tendency to decrease compared to the RNI treatment. Thirst level in the RSF treatment during feeding was approximately 31.3% less than the RNI treatment. Upon the completion of the 2 h feeding period, cumulative feed intake in the RSF treatment was significantly larger (19.7%) than the RNI treatment. The results suggest that a suppression of dry forage intake during the early stages of feeding in goats is partly caused by feeding induced hypovolemia, which is produced by the accelerated secretion of parotid saliva.

• Asian-Australasian Journal of Animal Sciences
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• 제29권11호
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• pp.1616-1624
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• 2016

The aim of present experiment was to investigate the effect of protein reduction in commercial broiler chicken rations with incorporation of de-oiled rice bran (DORB) and supplementation of limiting amino acids (valine, isoleucine, and/or tryptophan) with ration formulation either on total amino acid (TAA) or standardized ileal digestible amino acids (SIDAA). The experimental design consisted of $T_1$, TAA control; $T_2$ and $T_3$, 0.75% and 1.5% protein reduction by 3% and 6% DORB incorporation, respectively by replacing soybean meal with supplemental limiting amino acids to meet TAA requirement; $T_4$, SIDAA control, $T_5$ and $T_6$, 0.75% and 1.5% protein reduction by DORB incorporation (3% and 6%) with supplemental limiting amino acids on SIDAA basis. A total of 360 dold fast growing broiler chicks (Vencobb-400) were divided into 36 homogenous groups of ten chicks each, and six dietary treatments described were allocated randomly with six replications. During 42 days trial, the feed intake was significantly (p<0.05) reduced by TAA factor compared to SIDAA factor and protein factor significantly (p<0.05) reduced the feed intake at 1.5% reduction compared to normal protein group. This was observed only during pre-starter phase but not thereafter. The cumulative body weight gain (BWG) was significantly (p<0.05) reduced in TAA formulations with protein step-down of 1.5% ($T_3$, 1,993 g) compared to control ($T_1$, 2,067 g), while under SIDAA formulations, BWG was not affected with protein reduction of 1.5% ($T_6$, 2,076 g) compared to $T_4$ (2,129 g). The feed conversion ratio (FCR) was significantly (p<0.05) reduced in both TAA and SIDAA formulations with 1.5% protein step-down ($T_3$, 1.741; $T_6$, 1.704) compared to respective controls ($T_1$, 1.696; $T_4$, 1.663). The SIDAA formulation revealed significantly (p<0.05) higher BWG (2,095 g) and better FCR (1.684) compared to TAA formulation (2,028 g; 1.721). Intake of crude protein and all limiting amino acids (SID basis) was higher in SIDAA group than TAA group with resultant higher nitrogen retention (4.438 vs 4.027 g/bird/d). The nitrogen excretion was minimized with 1.5% protein reduction (1.608 g/bird) compared to normal protein group (1.794 g/bird). The serum uric acid concentration was significantly reduced in $T_3$ (9.45 mg/dL) as compared to $T_4$ (10.75 mg/dL). All carcass parameters were significantly (p<0.05) higher in SIDAA formulation over TAA formulation and 1.5% protein reduction significantly reduced carcass, breast and thigh yields. In conclusion, the dietary protein can be reduced by 0.75% with TAA formulation and 1.5% with SIDAA formulation through DORB incorporation and supplementation of limiting amino acids and among formulations, SIDAA formulation was better than TAA formulation.

• Asian Pacific Journal of Cancer Prevention
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• 제13권3호
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• pp.901-907
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• 2012

Objective: Cumulative evidence suggests that MLH1, the key component in the mismatch pathway, plays an important role in human cancers. Two potential functional polymorphisms (-93G>A and I219V) of MLH1 have been implicated in cancer risk. The aim of this meta-analysis was to summarize the evidence for associations. Methods: Eligible studies were identified by searching the electronic literature PubMed, ScienceDirect and Embase databases for relevant reports and bibliographies. Studies were included if of case-control design investigating MLH1 polymorphisms (-93G>A and I219V) and cancer risk with sufficient raw data for analysis. Odds ratios (OR) and 95% confidence intervals (95% CI) were used to evaluate the strength of associations. Results: Our meta-analysis from 33 published case-control studies showed the variant A allele of -93G>A polymorphism to be associated with increased risk in all genetic models (AA vs. GG: OR = 1.22, 95% CI: 1.03-1.44), especially among non-Asians (AA vs. GG: OR = 1.28, 95% CI: 1.04-1.58). For the I219V polymorphism, however, there was no main effect associated with overall cancer risk in any genetic model. Conclusions: The meta-analysis suggested that the MLH1 -93G>A polymorphism may be a biomarker of cancer susceptibility. Large sample association studies and assessment of gene-to-gene as well as gene-to-environment interactions are required to confirm these findings.

• Asian-Australasian Journal of Animal Sciences
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• 제27권6호
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• pp.847-854
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• 2014

Different herbs and spices have been used as feed additives for various purposes in poultry production. This study was conducted to assess the effect of feed supplemented with black pepper (Piper nigrum), turmeric powder (Curcuma longa), coriander seeds (Coriandrum sativum) and their combinations on the performance of broilers. A total of 210 (Cobb) one-d-old chicks were divided into seven groups of 30 birds each. The treatments were: a control group received no supplement, 0.5% black pepper (T1), 0.5% turmeric powder (T2), 2% coriander seeds (T3), a mixture of 0.5% black pepper and 0.5% turmeric powder (T4), a mixture of 0.5% black pepper and 2% coriander seed (T5), and a mixture of 0.5% black pepper, 0.5% turmeric powder and 2% coriander seeds (T6). Higher significant values of body weight gain during the whole period of 5 weeks (p<0.001) were observed in broilers on T1, T3, T5, and T6 compared to control. Dietary supplements with T1, T2, T3, and T6 improved the cumulative G:F of broilers during the whole period of 5 weeks (p<0.001) compared with control. The dressing percentage and edible giblets were not influenced by dietary supplements, while higher values of relative weight of the liver (p<0.05) were obtained in T5 and T6 compared to control. The addition of feed supplements in T5 and T6 significantly increased serum total protein and decreased serum glucose, triglycerides and alkaline phosphatase concentrations compared with the control group (p<0.05). Broilers on T6 showed significant decrease in the serum glutamate pyruvate transaminase concentration (p<0.05) compared to control. The broilers having T5 and T6 supplemented feed had relatively greater antibody titre (p<0.001) at 35 d of age than control. It is concluded that dietary supplements with black pepper or coriander seeds or their combinations enhanced the performance and health status of broiler chickens.