• 생명과학회지
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• 제14권4호
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• pp.614-619
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• 2004

This study was undertaken to investigate the different responses of cultured plant cells to paraquat treatment and nucleus-DNA damage in relation to enzyme activity of superoxide dismutase (SOD). Furthermore, this study was also carried out to understand the antioxidative mechanism of plant cells to environmental stress. We selected two different species of plant cultured cells, Ipomoea batatas as high-SOD species and Lonicera japonica as low-SOD species. The total activity and specific activity of SOD in a chlorophyllous cell of I. batatas were 3,736 unit/gㆍfresh weight and 547 unit/mgㆍprotein, respectively, and those in L. japonica were 23 unit/gㆍfresh weight and 13 unit/mgㆍprotein, respectively SOD activity in chlorophyllous I. batatas cells reached its maximum level at 10 to 15 days after subculture, whereas that in L. japonica remained at a very low SOD level during the whole period of subculture. In comparison to L. japonica, I. batatas, a high-SOD species, showed high tolerance to paraquat 10 and 50 mg/l treatment in terms of cell viability and electrolyte leakage. Based on the result of comet assay, the nucleus-DNA damage of two species by paraquat 50 mg/l treatment was not significantly different. However, I. batatas cells repaired their damaged DNA more effectively than the cells of the low-SOD species, L. japonica.

• 한국잡초학회지
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• 제11권2호
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• pp.137-141
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• 1991

녹화된 cal1us의 제초제 paraquat와 diuron에 대한 반응을 요약하면 다음과 같다. LS 기본(基本) 배지(培地)에 sucrose 농도(濃度) 1.0%, NAA $10^{-5}$ M, BA $10^{-6}$ M을 첨가(添加)하여 paraquat를 처리(處理) 하였을때 농도(濃度)가 $10^{-6}$ M에 서 $10^{-4}$ M으로 증가(增加) 할수록 calli의 증식(增殖)이 억제(抑制)되고 엽록소(葉綠素) 함량(含量)이 감소(減少)되었다. $10^{-4}$ M에서는 완전(完全) 고사(枯死)하였으며 $CO_2$가 공급(供給)되는 조건(條件)에서 억제(抑制) 정도가 높았다. 제초제(除草劑) diuron 처리(處理) 시(時)에도 $10^{-6}$ M에서 $10^{-3}$M으로 농도(濃度)가 증가(增加) 할수록 callus 증식(增殖)이 억제(抑制)되었으며 sucrose농도(濃度) 2.0% 때 보다 1.0%에서 훨씬 높은 억제(抑制) 경향(傾向)을 보였다.