• Title/Summary/Keyword: culture-independent

Search Result 827, Processing Time 0.032 seconds

A Critical Study about the Correlation between the Body without Organ and Blurring Boundary Fashion - Focusing on Philosophical and Sociological Discourses about the Body and the Desire - (기관 없는 신체와 탈영토화 된 패션 사이의 상관관계에 대한 비판적 고찰 - 철학적, 사회학적 신체 담론과 욕망이론을 중심으로 -)

  • Yang, Hee-Young;Lee, Myung-Hee
    • The Research Journal of the Costume Culture
    • /
    • v.18 no.5
    • /
    • pp.824-841
    • /
    • 2010
  • In 21st century, subordinated relationship of fashion from the body has been disappearing, and fashion is understood as variable and creative field. This research aims at analysis about the relationship between the body and fashion depending on the theories about the authority and the desire. So, this utilizes between theoretical study and empirical analysis. For concentrated study, research period limits from 2000 to the present time 2010. Contemporary body and fashion have being changed into various forms and values, become complex and de-territory. Especially, body is symbol of ambivalence eroticism that gives point to sexual property, and the object of fetishism and machine having a desire. This study's purpose draw a parallel with between the limits of contemporary body that couldn't be rid of the capital and desire, and the liberty of fashion that escape from the body's influence has being changed independent and fluid space. This research's results are as follow as; contemporary de-territory fashion is expressed as 1) symbol of the object and physical material property, 2) self-transcendental instrument fashion, 3) independent spatial molding, 4) de-centering fashion.

Comparative Analysis of Bacterial Diversity in the Intestinal Tract of Earthworm (Eisenia fetida) using DGGE and Pyrosequencing (DGGE 방법과 Pyrosequencing 방법을 이용한 지렁이 장내미생물의 다양성 분석)

  • Kim, Eun-Sung;Hong, Sung-Wook;Chung, Kun-Sub
    • Microbiology and Biotechnology Letters
    • /
    • v.39 no.4
    • /
    • pp.374-381
    • /
    • 2011
  • The beneficial effects of Eisenia fetida on soil properties have been attributed to their interaction with soil microorganisms. The bacterial diversity of the intestinal tract of E. fetida was investigated by culture-dependent and culture-independent methods including denaturing gradient gel electrophoresis (DGGE) and pyrosequencing analyses. In a pure culture, Lysinibacillus fusiformis (51%), Bacillus cereus (30%), Enterobacter aerogenes (21%), and L. sphaericus (15%) were identified as the dominant microorganisms. In the DGGE analyses, B. cereus (15.1%), Enterobacter sp. (13.6%), an uncultured bacterium (13.1%), and B. stearothermophilus (7.8%) were identified as the dominant microorganisms. In the pyrosequencing analyses, Microbacterium soli (26%), B. cereus (10%), M. esteraromaticum (6%), and Frigoribacterium sp. (6%) were identified as the dominant microorganisms. The other strains identified were Aeromonas sp., Pseudomonas sp., Borrelia sp., Cellulosimicrobium sp., Klebsiella sp., and Leifsonia sp. The results illustrate that culture independent methods are better able to detect unculturable microorganisms and a wider range of species, as opposed to isolation by culture dependent methods.

A Library As a Publishing Platform - on collaborative relationship between libraries and publishers - (출판 플랫폼으로서의 도서관 - 독립출판을 통해 바라본 출판과 도서관의 상생 -)

  • Chung, Jun Min
    • Journal of Korean Library and Information Science Society
    • /
    • v.49 no.4
    • /
    • pp.21-51
    • /
    • 2018
  • In the midst of the crisis in the publishing industry, the library was brought up as a facilitator. However, the premise is that libraries cannot revive the publishing industry simply as a catalyst for promoting reading culture. Rather, the publishing industry presented an expanded and regenerative system of publishing contents as a model for the promotion of publishing by embracing the active production and demand of readers based on libraries. In that process, we designed an independent publishing platform in the form of library/publishing collaboration (cooperation) and proposed a distribution and reproduction model.

On the Study of Textual Classics and Artistic Creation - Taking Buddhist Art Dunhuang Grottoes as an Example

  • Liu Tingting
    • International Journal of Advanced Culture Technology
    • /
    • v.11 no.3
    • /
    • pp.205-210
    • /
    • 2023
  • Stone cave paintings are continuous interactions as independent mediums in places such as text, images and stone cave architecture. Unlike Buddha statues, the narrative of the text always fascinates and guides the viewer to the timeliness of the image, that is, the narrative. In particular, in Buddhist art, Buddha statues are never simple images, and murals are never simple paintings. Before the Tang Dynasty, most unknown artists were artisans, and many artists still worked on murals in temples and palaces, and independent paintings such as scrolls and sides became an important form of painting after the Tang Dynasty, changing the mechanism of painting creation. In this paper, the graphic creation process prioritizes dedication and service, but we can still feel the creativity of the painters strongly. The historical resources of how to paint these paintings, the clues to the copies, and the precursor to the foreground, encourage the painters to constantly try to resemble each other and discover problems...Therefore, in this paper, it was confirmed that reinvention and creativity are very important, and that Dunhuang Buddhist art is the basis for artists' creation and the source of vitality.

Research on Four Variables toward the Effective Integration of Culture in the EFL Class of Korea

  • Roh, Seung-Bin
    • English Language & Literature Teaching
    • /
    • v.11 no.2
    • /
    • pp.91-110
    • /
    • 2005
  • Many Korean EFL (English as a Foreign Language) students do not have sufficient opportunity to develop cultural knowledge and information in their classrooms. EFL teachers also tend to ignore the teaching of culture. Even though culture is taught, it simply tends to deliver "fact-only" information from the viewpoint of a "tourist level rather than cultural awareness by comparing native with target cultural references. Teaching target cultural knowledge and information should be delivered within the native cultural frame, and teaching of culture must be an integral part of teaching and learning English. The research methodology was quantitative. Quantitative data was gathered from 83 Korean EFL teachers and 286 EFL students by questionnaire. Findings indicated that three of these independent variables (cultural inequality, English-only instruction, and Unoism) were significantly and inversely related to integration of culture.

  • PDF

The Study about the Job Satisfaction and Independent Establishment of Physical Therapy of Working Physical Therapist in Gyeongnam Region (경남지역 물리치료사의 직무만족도 및 자립개설에 대한 조사연구)

  • Lee, Jun-cheol
    • The Journal of the Convergence on Culture Technology
    • /
    • v.4 no.4
    • /
    • pp.123-133
    • /
    • 2018
  • This study aimed to suggest basic data required to improve the awareness of physical therapy and examine physical therapists' awareness of the independent establishment of physical therapy clinics. To do so, 175 physical therapists in Gyeongnam areas were surveyed. Their general characteristics were statistically analyzed using SPSS 10.0, and their current workplace, working conditions and job satisfaction were analyzed using chi square distributions. The awareness of independent establishment was analyzed using the Excel program. The statistical significance (a) in this study was set at 0.05. Their interest in physical therapy was surveyed by gender, and 52.2% of males showed interest, higher than that of females. The satisfaction level of those who work in general hospitals with self-development was 32.8%, higher than that of those who work in clinics. To the question about independent establishment, 83.4% agreed to independent establishment, and 57.5% answered that the first thing to do before independent establishment is acquiring professional theoretical knowledge and clinical treatment skills. In terms of the areas of establishment, 45.2% answered physical therapy for the musculoskeletal system, and 39.7% answered that the awareness of doctors is the biggest obstacle to independent establishment. It will be necessary to ensure physical therapists acquire professional theoretical knowledge and clinical treatment skills and to improve awareness between physical therapists and doctors. In addition, it is considered that departments related to physical therapy need to be unified as a four-year course, and the independent establishment of physical therapy clinics is required to be legalized.

The characteristics of feminist fashion brands and female images (페미니스트 패션 브랜드의 특성과 여성 이미지)

  • Im, MinJung
    • The Research Journal of the Costume Culture
    • /
    • v.26 no.3
    • /
    • pp.471-484
    • /
    • 2018
  • This study selected fashion brands claiming to advocate feminism to analyze their characteristics and female images. For the study's data, online foreign feminist fashion brands were sifted from March 2017 to January 2018 and 28 clothing brands were selected. The study's results show that feminist fashion brands aim at the demassification and individualization of fashion products to be more inclusive of individuals' physical characteristics and diversity. Additionally, feminist brands entice consumption through communication and participation in online communities and through the value of social coexistence. The essential female image produced by feminist fashion brands deconstructs a socially idealized female image and expresses a sense of self-body positivity. In turn, the concept of self-body positivity is communicated through natural images of independent women with distinct identities based on differences in race, culture, and sexual orientation. Moreover, feminist fashion brands produce social images featuring independent women using active wear to engage in social activities. Casual wear is also used to reflect active women, while mannish looks and power suits express women's social status and professional abilities. Ultimately, these offer functionally active and rational images, combined with female images featuring long hair and makeup. Yet another type of female image seeks to create a new vision of women as diverse due to their various cultures, countries of origin, races, and individual tastes. These new images express women's physical differences, distinct identities, and diversity while simultaneously deconstructing pre-existing forms of clothing.

Analysis techniques for fermented foods microbiome (발효식품의 마이크로바이옴 분석 기술)

  • Cha, In-Tae;Seo, Myung-ji
    • Food Science and Industry
    • /
    • v.50 no.1
    • /
    • pp.2-10
    • /
    • 2017
  • Human have eaten various traditional fermented foods for a numbers of million years for health benefit as well as survival. The beneficial effects of fermented foods have been resulted from complex microbial communications within the fermented foods. Therefore, the holistic approaches for individual identification and complete microbial profiling involved in their communications have been of interest to food microbiology fields. Microbiome is the ecological community of microorganisms that literally share our environments including foods as well as human body. However, due to the limitation of culture-dependent methods such as simple isolations of just culturable microorganisms, the culture-independent methods have been consistently developed, resulting in new light on the diverse non-culturable and hitherto unknown microorganisms, and even microbial communities in the fermented foods. For the culture-independent approaches, the food microbiome has been deciphered by employing various molecular analysis tools such as fluorescence in situ hybridization, quantitative PCR, and denaturing gradient gel-electrophoresis. More recently, next-generation-sequencing (NGS) platform-based microbiome analysis has been of interest, because NGS is a powerful analytical tool capable of resolving the microbiome in respect to community structures, dynamics, and activities. In this overview, the development status of analysis tools for the fermented food microbiome is covered and research trend for NGS-based food microbiome analysis is also discussed.

Duplex dPCR System for Rapid Identification of Gram-Negative Pathogens in the Blood of Patients with Bloodstream Infection: A Culture-Independent Approach

  • Shin, Juyoun;Shin, Sun;Jung, Seung-Hyun;Park, Chulmin;Cho, Sung-Yeon;Lee, Dong-Gun;Chung, Yeun-Jun
    • Journal of Microbiology and Biotechnology
    • /
    • v.31 no.11
    • /
    • pp.1481-1489
    • /
    • 2021
  • Early and accurate detection of pathogens is important to improve clinical outcomes of bloodstream infections (BSI), especially in the case of drug-resistant pathogens. In this study, we aimed to develop a culture-independent digital PCR (dPCR) system for multiplex detection of major sepsis-causing gram-negative pathogens and antimicrobial resistance genes using plasma DNA from BSI patients. Our duplex dPCR system successfully detected nine targets (five bacteria-specific targets and four antimicrobial resistance genes) through five reactions within 3 hours. The minimum detection limit was 50 ag of bacterial DNA, suggesting that 1 CFU/ml of bacteria in the blood can be detected. To validate the clinical applicability, cell-free DNA samples from febrile patients were tested with our system and confirmed high consistency with conventional blood culture. This system can support early identification of some drug-resistant gram-negative pathogens, which can help improving treatment outcomes of BSI.